Evaluation of adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis.
Abstract: The purpose of this study was the assessment of clinical, biochemical, and histologic effects of intraarticular administered adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis. Osteoarthritis was induced arthroscopically in the middle carpal joint of all horses, the contralateral joint being sham-operated. All horses received treatment on Day 14. Eight horses received placebo treatment and eight horses received adipose-derived stromal vascular fraction in their osteoarthritis-affected joint. The final eight horses were treated the in osteoarthritis-affected joint with bone marrow-derived mesenchymal stem cells. Evaluations included clinical, radiographic, synovial fluid analysis, gross, histologic, histochemical, and biochemical evaluations. No adverse treatment-related events were observed. The model induced a significant change in all but two parameters, no significant treatment effects were demonstrated, with the exception of improvement in synovial fluid effusion PGE2 levels with bone marrow-derived mesenchymal stem cells when compared to placebo. A greater improvement was seen with bone marrow-derived mesenchymal stem cells when compared to adipose-derived stromal vascular fraction and placebo treatment. Overall, the findings of this study were not significant enough to recommend the use of stem cells for the treatment of osteoarthritis represented in this model.
Publication Date: 2009-06-23 PubMed ID: 19544397DOI: 10.1002/jor.20933Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigated the effectiveness of two types of stem cells, adipose-derived stromal vascular fraction and bone marrow-derived mesenchymal stem cells, in treating osteoarthritis. Despite observing some improvements, the results weren’t significant enough to advise their use in treating osteoarthritis.
Research Methodology
- The aim of the study was to test the effects of injecting two types of stem cells – adipose-derived stromal vascular fraction (AD-SVF) and bone marrow-derived mesenchymal stem cells (BM-MSCs) – directly into the joints of horses suffering from osteoarthritis.
- The researchers artificially induced osteoarthritis in the middle carpal joint of all horses involved in the study.
- The contralateral joint, the joint on the opposite side, of each horse was sham-operated, meaning it was prepared and treated as if it was going to be operated on, but the actual surgical procedure was not carried out.
- Treatment began two weeks into the study. The horses were divided into three groups – one group was given a placebo, one was treated with AD-SVF, and the last with BM-MSCs.
Research Findings
- All treatment procedures were accomplished without causing any adverse reactions or side effects.
- The induced osteoarthritis resulted in significant changes to nearly all measured parameters, suggesting the induction procedure was effective.
- No significant treatment effects were observed overall, except for a decrease in the level of synovial fluid effusion PGE2 with the use of BM-MSCs, compared to the placebo treatment.
- BM-MSCs were also seen to be more effective at reducing synovial fluid effusion PGE2 levels than AD-SVF.
- However, the improvements recorded were not marked enough to conclusively suggest the use of stem cell therapy for osteoarthritis treatment.
Conclusions
- While the study observed some minimal positive effects of BM-MSCs, particularly in comparison to both the placebo and AD-SVF treatments, the overall findings do not strongly advocate for the use of stem cells in treating osteoarthritis in this model.
Cite This Article
APA
Frisbie DD, Kisiday JD, Kawcak CE, Werpy NM, McIlwraith CW.
(2009).
Evaluation of adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis.
J Orthop Res, 27(12), 1675-1680.
https://doi.org/10.1002/jor.20933 Publication
Researcher Affiliations
- Equine Orthopaedic Research Center, Department of Clinical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, Colorado 80523, USA. dfrisbie@colostate.edu
MeSH Terms
- Adipocytes / cytology
- Animals
- Arthritis, Experimental
- Arthroscopy
- Cells, Cultured
- Hematopoietic Stem Cell Transplantation / methods
- Horse Diseases / therapy
- Horses
- Mesenchymal Stem Cell Transplantation / methods
- Mesenchymal Stem Cells / cytology
- Osteoarthritis / diagnostic imaging
- Osteoarthritis / pathology
- Osteoarthritis / therapy
- Physical Conditioning, Animal
- Radiography
- Stifle / pathology
- Stifle / physiopathology
- Stifle / surgery
- Stromal Cells / cytology
- Synovial Fluid / cytology
- Synovial Fluid / metabolism
- Synovial Fluid / physiology
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