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Animals : an open access journal from MDPI2020; 10(5); doi: 10.3390/ani10050812

Evolution of In Vitro Antimicrobial Susceptibility of Equine Clinical Isolates in France between 2016 and 2019.

Abstract: The present study described the evolution of antimicrobial resistance in equine pathogens isolated from 2016 to 2019. A collection of 7806 bacterial isolates were analysed for their in vitro antimicrobial susceptibility using the disk diffusion method. The most frequently isolated pathogens were group C Streptococci (27.0%), Escherichia coli (18.0%), Staphylococcus aureus (6.2%), Pseudomonas aeruginosa (3.4%), Klebsiella pneumoniae (2.3%) and Enterobacter spp. (2.1%). The majority of these pathogens were isolated from the genital tract (45.1%, n = 3522). With the implementation of two French national plans (named ECOANTIBIO 1 and 2) in 2012-2016 and 2017-2021, respectively, and a reduction in animal exposure to veterinary antibiotics, our study showed decreases in the resistance of group C Streptococci, Klebsiella pneumoniae and Escherichia coli against five classes, four classes and one class of antimicrobials tested, respectively. However, Staphylococcus aureus, Escherichia coli and Enterobacter spp. presented an increased resistance against all the tested classes, excepted for two fifths of E. coli. Moreover, the percentages of multi-drug resistant strains of Staphylococcus aureus and Enterobacter spp. also increased from 24.5% to 37.4% and from 26.3% to 51.7%, respectively. The data reported here are relevant to equine practitioners and will help to improve knowledge related to antimicrobial resistance in common equine pathogens.
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Publication Date: 2020-05-07 PubMed ID: 32392891PubMed Central: PMC7278474DOI: 10.3390/ani10050812Google Scholar: Lookup
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Summary

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The research deals with the assessment of the evolution of antimicrobial resistance in common bacterial pathogens found in horses from 2016 to 2019 in France.

Research Methodology

  • The scientists analyzed an extensive collection of 7806 bacterial isolates for their in vitro antimicrobial susceptibility. The examination was conducted using a standard method known as disk diffusion.
  • The most frequently isolated pathogens included group C Streptococci (27.0%), Escherichia coli (18.0%), Staphylococcus aureus (6.2%), Pseudomonas aeruginosa (3.4%), Klebsiella pneumoniae (2.3%), and Enterobacter spp. (2.1%). Most of these bacteria were isolated from horse genital tracts.
  • The data collection and analysis period coincided with the implementation of two French national plans, ECOANTIBIO 1 (2012 – 2016) and ECOANTIBIO 2 (2017 – 2021), aimed at reducing animal exposure to veterinary antibiotics.

Key Findings

  • Following the implementation of the ECOANTIBIO plans, the researchers observed decreased resistance in several key equine pathogens. Resistance in group C Streptococci, Klebsiella pneumoniae, and Escherichia coli declined against five, four, and one classes of antimicrobials tested, respectively.
  • On the other hand, other bacteria like Staphylococcus aureus, Escherichia coli, and Enterobacter spp. showed an increase in resistance against most of the tested classes, except for a fifth of E.coli isolates.
  • Strikingly, the percentages of multi-drug resistant strains of Staphylococcus aureus and Enterobacter spp. exhibited significant growth, jumping from 24.5% to 37.4% and from 26.3% to 51.7%, respectively.

Significance of the Study

  • The study presents valuable data on the evolution of antimicrobial resistance in common horse pathogens. It follows the implementation of national plans aimed at reducing veterinary antibiotic use, providing insight into their effectiveness.
  • Despite observed successes, the research underscores the increasingly resistant nature of some bacteria to the spectrum of tested antimicrobials.
  • The information from the study is crucial for equine practitioners. It can inform better treatment strategies and encourage further research into antimicrobial resistance in common equine pathogens.

Cite This Article

APA
Léon A, Castagnet S, Maillard K, Paillot R, Giard JC. (2020). Evolution of In Vitro Antimicrobial Susceptibility of Equine Clinical Isolates in France between 2016 and 2019. Animals (Basel), 10(5). https://doi.org/10.3390/ani10050812

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 10
Issue: 5

Researcher Affiliations

Léon, Albertine
  • LABÉO Frank Duncombe, 14053 CAEN, France.
  • Normandie Univ, UNICAEN, U2RM, 14033 Caen, France.
Castagnet, Sophie
  • LABÉO Frank Duncombe, 14053 CAEN, France.
Maillard, Karine
  • LABÉO Frank Duncombe, 14053 CAEN, France.
Paillot, Romain
  • LABÉO Frank Duncombe, 14053 CAEN, France.
  • Normandie Univ, UNICAEN, Biotargen, 14033 Caen, France.
Giard, Jean-Christophe
  • Normandie Univ, UNICAEN, U2RM, 14033 Caen, France.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 15 times.
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