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Home / Equine Research Bank / Microb Drug Resist / Evolution of multidrug-resistant Staphylococcus aureus infec...
Microbial drug resistance (Larchmont, N.Y.)2011; 17(3); 471-478; doi: 10.1089/mdr.2010.0188

Evolution of multidrug-resistant Staphylococcus aureus infections in horses and colonized personnel in an equine clinic between 2005 and 2010.

Abstract: A total of 70 Staphylococcus aureus isolates from postoperative infections in hospitalized horses were isolated between January 2005 and January 2011. Among them, 12 isolates were methicillin-susceptible S. aureus (MSSA), 18 were borderline-oxacillin-resistant S. aureus (BORSA), and 40 were methicillin-resistant S. aureus (MRSA). During the same period, the equine clinic personnel were screened for nasal carriage of BORSA and MRSA. Genotyping revealed that BORSA ST1(MLST)-t2863(spa) isolates were responsible for most equine infections and were the main isolates found in colonized members of the personnel between 2005 and 2007, and that in 2007, MRSA ST398-t011-IVa(SCCmec) emerged in infection sites and personnel, replacing BORSA. Besides decreased susceptibility to oxacillin, all MRSA and BORSA of these two major clonal lineages displayed resistance to gentamicin and kanamycin conferred by the aac(6')-Ie-aph(2')-Ia gene and to trimethoprim conferred by dfr(K) in MRSA and dfr(A) in BORSA. All MRSA had additional resistance to tetracycline conferred by tet(M), whereas BORSA generally also display resistance to streptomycin conferred by str. The number of hospital-acquired MRSA infections in horses could be limited after the introduction of basic hygiene measures and personnel decolonization. Two MRSA carriers could not be decolonized using mupirocin, and a year after decolonization, additional members were recolonized with MRSA. Hygiene measures should, therefore, be maintained to limit the transmission of S. aureus between personnel and horses.
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Publication Date: 2011-08-31 PubMed ID: 21875361DOI: 10.1089/mdr.2010.0188Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examined the evolution of multi-drug resistant Staphylococcus aureus (S. aureus) infections in horses at an equine clinic from 2005-2010. 70 S. aureus strains were analyzed from post-surgery infections in the horses, 40 of those were methicillin-resistant (MRSA), 18 were borderline-oxacillin-resistant (BORSA), and remaining 12 were methicillin-susceptible (MSSA). Staff at the clinic were also tested for S. aureus carriage. The study found that a shift in infection and nasal carriage in both horses and staff from BORSA to MRSA, particularly after 2007. Hygiene measures and staff decolonization effectively limited hospital-acquired MRSA infections.

Method and Results

  • From January 2005 to 2011, the researchers isolated 70 S. aureus from horses who had undergone surgery. They categorized these isolates into three groups — methicillin-susceptible S. aureus (MSSA), borderline-oxacillin-resistant S. aureus (BORSA), and methicillin-resistant S. aureus (MRSA).
  • The researchers also tested the nasal passages of the equine clinic staff for carriage of MRSA and BORSA.
  • Using genotyping, they found that BORSA ST1(MLST)-t2863(spa) isolates were the most responsible for infections in horses and were also present in a significant number of staff members between 2005 and 2007.
  • From 2007, the MRSA ST398-t011-IVa(SCCmec) strain emerged in both the horses and clinic staff, displacing the BORSA strains.

Additional findings

  • All BORSA and MRSA isolates registered a reduced susceptibility to oxacillin and, in addition, were found to be resistant to other antibiotics such as kanamycin and gentamicin. The resistance was attributed to the aac(6′)-Ie-aph(2′)-Ia gene.
  • MRSA strains displayed resistance to trimethoprim due to the dfr(K) gene, whereas in BORSA, this resistance was linked to the dfr(A) gene.
  • Moreover, all MRSA isolates also showed resistance to tetracycline due to the tet(M) gene. BORSA isolates typically showed resistance to streptomycin as well.

Management of the infection

  • Introduction of basic hygiene measures and decolonization of personnel greatly reduced the number of hospital-acquired MRSA infections in horses.
  • Two personnel remained MRSA-positive despite decolonization efforts with mupirocin. These staff members and a few others were recolonized with MRSA after a year.
  • The researchers concluded hygiene measures should be continued in order to minimize the transmission of MRSA and BORSA between staff and horses.

Cite This Article

APA
Sieber S, Gerber V, Jandova V, Rossano A, Evison JM, Perreten V. (2011). Evolution of multidrug-resistant Staphylococcus aureus infections in horses and colonized personnel in an equine clinic between 2005 and 2010. Microb Drug Resist, 17(3), 471-478. https://doi.org/10.1089/mdr.2010.0188

Publication

Microbial drug resistance (Larchmont, N.Y.)
ISSN: 1931-8448
NlmUniqueID: 9508567
Country: United States
Language: English
Volume: 17
Issue: 3
Pages: 471-478

Researcher Affiliations

Sieber, Sandra
  • Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland.
Gerber, Vinzenz
    Jandova, Vendula
      Rossano, Alexandra
        Evison, John Marc
          Perreten, Vincent

            MeSH Terms

            • Animals
            • Bacterial Typing Techniques / methods
            • Bacterial Typing Techniques / veterinary
            • Cross Infection / microbiology
            • Cross Infection / veterinary
            • Drug Resistance, Multiple, Bacterial / genetics
            • Genotype
            • Horse Diseases / microbiology
            • Horses
            • Hospitals, Animal
            • Humans
            • Methicillin-Resistant Staphylococcus aureus / genetics
            • Methicillin-Resistant Staphylococcus aureus / isolation & purification
            • Microbial Sensitivity Tests / methods
            • Microbial Sensitivity Tests / veterinary
            • Oxacillin / pharmacology
            • Staphylococcal Infections / microbiology
            • Staphylococcal Infections / veterinary
            • Staphylococcus aureus / genetics

            Citations

            This article has been cited 33 times.
            1. Jahnen J, Cuny C, Witte W, Ehricht R, Monecke S, Hanke D, Ahrens T, Leal M, Costa SS, Couto I, Schwarz S, Feßler AT. Characterization of Staphylococcus aureus CC1 and CC1660 of Human and Equine Origin. Antibiotics (Basel) 2025 Oct 27;14(11).
              doi: 10.3390/antibiotics14111082pubmed: 41301578google scholar: lookup
            2. Allano M, Arsenault J, Archambault M, Fairbrother JH, Sauvé F. Prevalence and Risk Factors of Staphylococcus aureus Nasal Colonization in Horses Admitted to a Veterinary Teaching Hospital. J Vet Intern Med 2025 May-Jun;39(3):e70027.
              doi: 10.1111/jvim.70027pubmed: 40135807google scholar: lookup
            3. Caddey B, Fisher S, Barkema HW, Nobrega DB. Companions in antimicrobial resistance: examining transmission of common antimicrobial-resistant organisms between people and their dogs, cats, and horses. Clin Microbiol Rev 2025 Mar 13;38(1):e0014622.
              doi: 10.1128/cmr.00146-22pubmed: 39853095google scholar: lookup
            4. Engku Abd Rahman ENS, Irekeola AA, Yamin D, Elmi AH, Chan YY. Charting the global footprint of borderline oxacillin-resistant Staphylococcus aureus (BORSA): the first systematic review and meta-analysis. PeerJ 2024;12:e18604.
              doi: 10.7717/peerj.18604pubmed: 39703916google scholar: lookup
            5. Abdank L, Loncaric I, Braun SD, Müller E, Monecke S, Ehricht R, Krametter-Frötscher R. Characterizing Methicillin-Resistant Staphylococcus spp. and Extended-Spectrum Cephalosporin-Resistant Escherichia coli in Cattle. Animals (Basel) 2024 Nov 25;14(23).
              doi: 10.3390/ani14233383pubmed: 39682349google scholar: lookup
            6. Fernandez JE, Egli A, Overesch G, Perreten V. Time-calibrated phylogenetic and chromosomal mobilome analyses of Staphylococcus aureus CC398 reveal geographical and host-related evolution. Nat Commun 2024 Jul 1;15(1):5526.
              doi: 10.1038/s41467-024-49644-9pubmed: 38951499google scholar: lookup
            7. Agrawal S, Mangera Z, Murray RL, Howle F, Evison M. Successes and Challenges of Implementing Tobacco Dependency Treatment in Health Care Institutions in England. Curr Oncol 2022 May 20;29(5):3738-3747.
              doi: 10.3390/curroncol29050299pubmed: 35621689google scholar: lookup
            8. Nielsen SS, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar C, Herskin M, Michel V, Miranda Chueca MÁ, Padalino B, Pasquali P, Roberts HC, Spoolder H, Ståhl K, Velarde A, Viltrop A, Winckler C, Baldinelli F, Broglia A, Kohnle L, Alvarez J. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): antimicrobial-resistant Staphylococcus aureus in cattle and horses. EFSA J 2022 May;20(5):e07312.
              doi: 10.2903/j.efsa.2022.7312pubmed: 35582361google scholar: lookup
            9. Kaiser-Thom S, Gerber V, Collaud A, Hurni J, Perreten V. Prevalence and WGS-based characteristics of Staphylococcus aureus in the nasal mucosa and pastern of horses with equine pastern dermatitis. BMC Vet Res 2022 Feb 24;18(1):79.
              doi: 10.1186/s12917-021-03053-ypubmed: 35209904google scholar: lookup
            10. Sawhney SS, Ransom EM, Wallace MA, Reich PJ, Dantas G, Burnham CD. Comparative Genomics of Borderline Oxacillin-Resistant Staphylococcus aureus Detected during a Pseudo-outbreak of Methicillin-Resistant S. aureus in a Neonatal Intensive Care Unit. mBio 2022 Feb 22;13(1):e0319621.
              doi: 10.1128/mbio.03196-21pubmed: 35038924google scholar: lookup
            11. Roudaud M, Allano M, Fairbrother JH, Sauvé F. A retrospective study on methicillin-resistant Staphylococcus spp. isolated from horses admitted to a Canadian veterinary teaching hospital between 2008 and 2018. Can Vet J 2020 Nov;61(11):1197-1202.
              pubmed: 33149358
            12. Schmidt JS, Kuster SP, Nigg A, Dazio V, Brilhante M, Rohrbach H, Bernasconi OJ, Büdel T, Campos-Madueno EI, Gobeli Brawand S, Schuller S, Endimiani A, Perreten V, Willi B. Poor infection prevention and control standards are associated with environmental contamination with carbapenemase-producing Enterobacterales and other multidrug-resistant bacteria in Swiss companion animal clinics. Antimicrob Resist Infect Control 2020 Jun 23;9(1):93.
              doi: 10.1186/s13756-020-00742-5pubmed: 32576281google scholar: lookup
            13. Schnepf A, Bienert-Zeit A, Ertugrul H, Wagels R, Werner N, Hartmann M, Feige K, Kreienbrock L. Antimicrobial Usage in Horses: The Use of Electronic Data, Data Curation, and First Results. Front Vet Sci 2020;7:216.
              doi: 10.3389/fvets.2020.00216pubmed: 32411737google scholar: lookup
            14. Potier JFN, Durham AE. Antimicrobial susceptibility of bacterial isolates from ambulatory practice and from a referral hospital. J Vet Intern Med 2020 Jan;34(1):300-306.
              doi: 10.1111/jvim.15685pubmed: 31849110google scholar: lookup
            15. Kittl S, Brodard I, Heim D, Andina-Pfister P, Overesch G. Methicillin-Resistant Staphylococcus aureus Strains in Swiss Pigs and Their Relation to Isolates from Farmers and Veterinarians. Appl Environ Microbiol 2020 Feb 18;86(5).
              doi: 10.1128/AEM.01865-19pubmed: 31836575google scholar: lookup
            16. Scholtzek AD, Hanke D, Walther B, Eichhorn I, Stöckle SD, Klein KS, Gehlen H, Lübke-Becker A, Schwarz S, Feßler AT. Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility. Toxins (Basel) 2019 Sep 13;11(9).
              doi: 10.3390/toxins11090535pubmed: 31540335google scholar: lookup
            17. Schwarz S, Feßler AT, Loncaric I, Wu C, Kadlec K, Wang Y, Shen J. Antimicrobial Resistance among Staphylococci of Animal Origin. Microbiol Spectr 2018 Jul;6(4).
              doi: 10.1128/microbiolspec.ARBA-0010-2017pubmed: 29992898google scholar: lookup
            18. de Jong NWM, Vrieling M, Garcia BL, Koop G, Brettmann M, Aerts PC, Ruyken M, van Strijp JAG, Holmes M, Harrison EM, Geisbrecht BV, Rooijakkers SHM. Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains. J Biol Chem 2018 Mar 23;293(12):4468-4477.
              doi: 10.1074/jbc.RA117.000599pubmed: 29414776google scholar: lookup
            19. Adams R, Smith J, Locke S, Phillips E, Erol E, Carter C, Odoi A. An epidemiologic study of antimicrobial resistance of Staphylococcus species isolated from equine samples submitted to a diagnostic laboratory. BMC Vet Res 2018 Feb 5;14(1):42.
              doi: 10.1186/s12917-018-1367-6pubmed: 29402294google scholar: lookup
            20. Bortolami A, Williams NJ, McGowan CM, Kelly PG, Archer DC, Corrò M, Pinchbeck G, Saunders CJ, Timofte D. Environmental surveillance identifies multiple introductions of MRSA CC398 in an Equine Veterinary Hospital in the UK, 2011-2016. Sci Rep 2017 Jul 14;7(1):5499.
              doi: 10.1038/s41598-017-05559-8pubmed: 28710350google scholar: lookup
            21. Boyle AG, Rankin SC, Duffee LA, Morris D. Prevalence of Methicillin-Resistant Staphylococcus aureus from Equine Nasopharyngeal and Guttural Pouch Wash Samples. J Vet Intern Med 2017 Sep;31(5):1551-1555.
              doi: 10.1111/jvim.14783pubmed: 28661019google scholar: lookup
            22. Cuny C, Abdelbary MMH, Köck R, Layer F, Scheidemann W, Werner G, Witte W. Methicillin-resistant Staphylococcus aureus from infections in horses in Germany are frequent colonizers of veterinarians but rare among MRSA from infections in humans. One Health 2016 Dec;2:11-17.
              doi: 10.1016/j.onehlt.2015.11.004pubmed: 28616471google scholar: lookup
            23. Guérin F, Fines-Guyon M, Meignen P, Delente G, Fondrinier C, Bourdon N, Cattoir V, Léon A. Nationwide molecular epidemiology of methicillin-resistant Staphylococcus aureus responsible for horse infections in France. BMC Microbiol 2017 May 3;17(1):104.
              doi: 10.1186/s12866-016-0924-zpubmed: 28468636google scholar: lookup
            24. Islam MZ, Espinosa-Gongora C, Damborg P, Sieber RN, Munk R, Husted L, Moodley A, Skov R, Larsen J, Guardabassi L. Horses in Denmark Are a Reservoir of Diverse Clones of Methicillin-Resistant and -Susceptible Staphylococcus aureus. Front Microbiol 2017;8:543.
              doi: 10.3389/fmicb.2017.00543pubmed: 28421046google scholar: lookup
            25. Koop G, Vrieling M, Storisteanu DM, Lok LS, Monie T, van Wigcheren G, Raisen C, Ba X, Gleadall N, Hadjirin N, Timmerman AJ, Wagenaar JA, Klunder HM, Fitzgerald JR, Zadoks R, Paterson GK, Torres C, Waller AS, Loeffler A, Loncaric I, Hoet AE, Bergström K, De Martino L, Pomba C, de Lencastre H, Ben Slama K, Gharsa H, Richardson EJ, Chilvers ER, de Haas C, van Kessel K, van Strijp JA, Harrison EM, Holmes MA. Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus. Sci Rep 2017 Jan 20;7:40660.
              doi: 10.1038/srep40660pubmed: 28106142google scholar: lookup
            26. Cuny C, Wieler LH, Witte W. Livestock-Associated MRSA: The Impact on Humans. Antibiotics (Basel) 2015 Nov 6;4(4):521-43.
              doi: 10.3390/antibiotics4040521pubmed: 27025639google scholar: lookup
            27. Steinman A, Masarwa S, Tirosh-Levy S, Gleser D, Kelmer G, Adler A, Carmeli Y, Schwaber MJ. Methicillin-Resistant Staphylococcus aureus spa Type t002 Outbreak in Horses and Staff at a Veterinary Teaching Hospital after Its Presumed Introduction by a Veterinarian. J Clin Microbiol 2015 Sep;53(9):2827-31.
              doi: 10.1128/JCM.00090-15pubmed: 26085620google scholar: lookup
            28. van Balen J, Mowery J, Piraino-Sandoval M, Nava-Hoet RC, Kohn C, Hoet AE. Molecular epidemiology of environmental MRSA at an equine teaching hospital: introduction, circulation and maintenance. Vet Res 2014 Mar 19;45(1):31.
              doi: 10.1186/1297-9716-45-31pubmed: 24641543google scholar: lookup
            29. Abdelbary MM, Wittenberg A, Cuny C, Layer F, Kurt K, Wieler LH, Walther B, Skov R, Larsen J, Hasman H, Fitzgerald JR, Smith TC, Wagenaar JA, Pantosti A, Hallin M, Struelens MJ, Edwards G, Böse R, Nübel U, Witte W. Phylogenetic analysis of Staphylococcus aureus CC398 reveals a sub-lineage epidemiologically associated with infections in horses. PLoS One 2014;9(2):e88083.
              doi: 10.1371/journal.pone.0088083pubmed: 24505386google scholar: lookup
            30. Mallardo K, Nizza S, Fiorito F, Pagnini U, De Martino L. A comparative evaluation of methicillin-resistant staphylococci isolated from harness racing-horses, breeding mares and riding-horses in Italy. Asian Pac J Trop Biomed 2013 Mar;3(3):169-73.
              doi: 10.1016/S2221-1691(13)60044-1pubmed: 23620832google scholar: lookup
            31. Crombé F, Argudín MA, Vanderhaeghen W, Hermans K, Haesebrouck F, Butaye P. Transmission Dynamics of Methicillin-Resistant Staphylococcus aureus in Pigs. Front Microbiol 2013;4:57.
              doi: 10.3389/fmicb.2013.00057pubmed: 23518663google scholar: lookup
            32. Van den Eede A, Martens A, Feryn I, Vanderhaeghen W, Lipinska U, Gasthuys F, Butaye P, Haesebrouck F, Hermans K. Low MRSA prevalence in horses at farm level. BMC Vet Res 2012 Nov 7;8:213.
              doi: 10.1186/1746-6148-8-213pubmed: 23134703google scholar: lookup
            33. Sobral D, Schwarz S, Bergonier D, Brisabois A, Feßler AT, Gilbert FB, Kadlec K, Lebeau B, Loisy-Hamon F, Treilles M, Pourcel C, Vergnaud G. High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources. PLoS One 2012;7(5):e33967.
              doi: 10.1371/journal.pone.0033967pubmed: 22567085google scholar: lookup
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