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Home / Equine Research Bank / Clin Vaccine Immunol / Experimental infection of neonatal foals with Rhodococcus eq...
Clinical and vaccine immunology : CVI2007; 14(6); 669-677; doi: 10.1128/CVI.00042-07

Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.

Abstract: Rhodococcus equi is a facultative intracellular pathogen that causes pneumonia in young foals but does not induce disease in immunocompetent adult horses. Clearance of R. equi depends mainly on gamma interferon (IFN-gamma) production by T lymphocytes, whereas the predominance of interleukin 4 (IL-4) is detrimental. Young foals, like neonates of many other species, are generally deficient in the ability to produce IFN-gamma. The objective of this study was to compare the cytokine profiles, as well as cell-mediated and antibody responses, of young foals to those of adult horses following intrabronchial challenge with R. equi. The lymphoproliferative responses of bronchial lymph node (BLN) cells to concanavalin A were significantly higher in foals than in adult horses. In contrast, adult horses had significantly higher lymphoproliferative responses to R. equi antigens than did foals. Infected foals had significantly lower IL-4 mRNA expression but significantly higher IFN-gamma expression and IFN-gamma/IL-4 ratio in R. equi-stimulated BLN lymphocytes than did infected adults. Infection with R. equi in foals resulted in a significant increase in the percentage of T lymphocytes and CD4(+) T lymphocytes in bronchoalveolar lavage fluid in association with a significant decrease in the percentage of these cell populations in BLNs. Infection of foals also resulted in a marked increase in serum immunoglobulin Ga (IgGa) and IgGb levels, resulting in concentrations in serum that were significantly higher than those of adult horses. This study demonstrates that the immune response to R. equi in foals is not biased toward IL-4 and is characterized by the predominant induction of IFN-gamma.
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Publication Date: 2007-04-04 PubMed ID: 17409222PubMed Central: PMC1951072DOI: 10.1128/CVI.00042-07Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research experimentally infects young foals with Rhodococcus equi, an intracellular pathogen, to study the immune response comparing to adult horses. The results showed that young foals, despite being generally deficient in producing interferon-gamma (IFN-gamma), displayed an adult-like IFN-gamma induction and lower interleukin 4 mRNA expression, driving a shift in the immune response.

Objective and Methodology

  • The main aim of this study was to scrutinize the differences in immune response, specifically the cytokine profiles cell-mediated, and antibody responses between young colts and adult horses when exposed to Rhodococcus equi.
  • The experiment involved intrabronchial injections of Rhodococcus equi in young foals to simulate a natural infection scenario.
  • Following the experimental infection, researchers examined the bronchial lymph node (BLN) cells’ responses, comparing them with those of adult horses.

Findings

  • The results indicated that the lymphoproliferative responses of BLN cells to concanavalin A in infected foals were significantly higher than in adult horses.
  • However, when exposed to Rhodococcus equi antigens, adult horses exhibited a stronger lymphoproliferative response.
  • Interestingly, infected foals demonstrated lower interleukin-4 (IL-4) mRNA expression, contrary to the conventional understanding that young mammals predominantly express IL-4.
  • Simultaneously, infected foals showed elevated expression of IFN-gamma, demonstrating an adult-like IFN-gamma induction response, resulting in a higher INF-gamma/IL-4 ratio compared to the infected adults.

Implications

  • This study reveals important nuances in the immunity of young foals when faced with Rhodococcus equi.
  • While young foals are generally deficient in producing IFN-gamma, the infection with Rhodococcus equi triggers an adult-like IFN-gamma induction.
  • Contrary to previous assumptions, the immune response in foals is not predominantly inclined towards IL-4 expression, providing new insight into foals immunity towards Rhodococcus equi.
  • The research can have significant implications for developing targeted treatments for Rhodococcus equi pneumonia in young foals, tailoring therapies according to their unique immune response.

Cite This Article

APA
Jacks S, Giguère S, Crawford PC, Castleman WL. (2007). Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction. Clin Vaccine Immunol, 14(6), 669-677. https://doi.org/10.1128/CVI.00042-07

Publication

Clinical and vaccine immunology : CVI
ISSN: 1556-6811
NlmUniqueID: 101252125
Country: United States
Language: English
Volume: 14
Issue: 6
Pages: 669-677

Researcher Affiliations

Jacks, Stephanie
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, P.O. Box 100136, 2015 S.W. 16th Ave., Gainesville, FL 32610, USA.
Giguère, Steeve
    Crawford, P Cynda
      Castleman, William L

        MeSH Terms

        • Actinomycetales Infections / immunology
        • Actinomycetales Infections / veterinary
        • Animals
        • Animals, Newborn
        • Bronchi / cytology
        • Bronchi / drug effects
        • Bronchoalveolar Lavage Fluid / cytology
        • Cell Proliferation / drug effects
        • Cells, Cultured
        • Concanavalin A / pharmacology
        • Horse Diseases / immunology
        • Horses
        • Immunoglobulin G / blood
        • Interferon-gamma / biosynthesis
        • Interferon-gamma / immunology
        • Interleukin-4 / immunology
        • Lymph Nodes / cytology
        • Lymph Nodes / drug effects
        • Lymphocyte Subsets / cytology
        • Mitogens / pharmacology
        • RNA, Messenger / metabolism
        • Rhodococcus equi / immunology
        • Rhodococcus equi / pathogenicity
        • T-Lymphocytes / cytology

        References

        This article includes 50 references
        1. Adkins B. Development of neonatal Th1/Th2 function. Int. Rev. Immunol. 19:157-171.
          pubmed: 10763707
        2. Adkins B. Neonatal T cell function. J. Pediatr. Gastroenterol. Nutr. 40(Suppl. 1):S5-S7.
          pubmed: 15805855
        3. Ahmed SA, Gogal RM Jr, Walsh JE. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J. Immunol. Methods 170:211-224.
          pubmed: 8157999
        4. Ainsworth DM, Grunig G, Matychak MB, Young J, Wagner B, Erb HN, Antczak DF. Recurrent airway obstruction (RAO) in horses is characterized by IFN-gamma and IL-8 production in bronchoalveolar lavage cells. Vet. Immunol. Immunopathol. 96:83-91.
          pubmed: 14522137
        5. Arlotti M, Zoboli G, Moscatelli GL, Magnani G, Maserati R, Borghi V, Andreoni M, Libanore M, Bonazzi L, Piscina A, Ciammarughi R. Rhodococcus equi infection in HIV-positive subjects: a retrospective analysis of 24 cases. Scand. J. Infect. Dis. 28:463-467.
          pubmed: 8953675
        6. Balson GA, Smith GD, Yager JA. Immunophenotypic analysis of foal bronchoalveolar lavage lymphocytes. Vet. Microbiol. 56:237-246.
          pubmed: 9226838
        7. Barrios C, Brandt C, Berney M, Lambert PH, Siegrist CA. Partial correction of the TH2/TH1 imbalance in neonatal murine responses to vaccine antigens through selective adjuvant effects. Eur. J. Immunol. 26:2666-2670.
          pubmed: 8921953
        8. Breathnach CC, Sturgill-Wright T, Stiltner JL, Adams AA, Lunn DP, Horohov DW. Foals are interferon gamma-deficient at birth. Vet. Immunol. Immunopathol. 112:199-209.
          pubmed: 16621024
        9. Cauchard J, Taouji S, Sevin C, Duquesne F, Bernabe M, Laugier C, Ballet JJ. Immunogenicity of synthetic Rhodococcus equi virulence-associated protein peptides in neonate foals. Int. J. Med. Microbiol. 296:389-396.
          pubmed: 16782401
        10. Chirino-Trejo JM, Prescott JF, Yager JA. Protection of foals against experimental Rhodococcus equi pneumonia by oral immunization. Can. J. Vet. Res. 51:444-447.
          pmc: PMC1255362pubmed: 3453264
        11. Cunha CW, McGuire TC, Kappmeyer LS, Hines SA, Lopez AM, Dellagostin OA, Knowles DP. Development of specific immunoglobulin Ga (IgGa) and IgGb antibodies correlates with control of parasitemia in Babesia equi infection. Clin. Vaccine Immunol. 13:297-300.
          pmc: PMC1391941pubmed: 16467341
        12. Donisi A, Suardi MG, Casari S, Longo M, Cadeo GP, Carosi G. Rhodococcus equi infection in HIV-infected patients. AIDS 10:359-362.
          pubmed: 8728038
        13. Flaminio MJ, Rush BR, Davis EG, Hennessy K, Shuman W, Wilkerson MJ. Characterization of peripheral blood and pulmonary leukocyte function in healthy foals. Vet. Immunol. Immunopathol. 73:267-285.
          pubmed: 10713340
        14. Flaminio MJ, Rush BR, Shuman W. Peripheral blood lymphocyte subpopulations and immunoglobulin concentrations in healthy foals and foals with Rhodococcus equi pneumonia. J. Vet. Intern. Med. 13:206-212.
          pubmed: 10357110
        15. Forsthuber T, Yip HC, Lehmann PV. Induction of TH1 and TH2 immunity in neonatal mice. Science 271:1728-1730.
          pubmed: 8596934
        16. Garton NJ, Gilleron M, Brando T, Dan HH, Giguère S, Puzo G, Prescott JF, Sutcliffe IC. A novel lipoarabinomannan from the equine pathogen Rhodococcus equi. Structure and effect on macrophage cytokine production. J. Biol. Chem. 277:31722-31733.
          pubmed: 12072437
        17. Giguère S, Gaskin JM, Miller C, Bowman JL. Evaluation of a commercially available hyperimmune plasma product for prevention of naturally acquired pneumonia caused by Rhodococcus equi in foals. J. Am. Vet. Med. Assoc. 220:59-63.
          pubmed: 12680449
        18. Giguère S, Hondalus MK, Yager JA, Darrah P, Mosser DM, Prescott JF. Role of the 85-kilobase plasmid and plasmid-encoded virulence-associated protein A in intracellular survival and virulence of Rhodococcus equi. Infect. Immun. 67:3548-3557.
          pmc: PMC116543pubmed: 10377138
        19. Giguère S, Wilkie BN, Prescott JF. Modulation of cytokine response of pneumonic foals by virulent Rhodococcus equi. Infect. Immun. 67:5041-5047.
          pmc: PMC96851pubmed: 10496876
        20. Goodman LB, Wagner B, Flaminio MJ, Sussman KH, Metzger SM, Holland R, Osterrieder N. Comparison of the efficacy of inactivated combination and modified-live virus vaccines against challenge infection with neuropathogenic equine herpesvirus type 1 (EHV-1). Vaccine 24:3636-3645.
          pubmed: 16513225
        21. Harvey RL, Sunstrum JC. Rhodococcus equi infection in patients with and without human immunodeficiency virus infection. Rev. Infect. Dis. 13:139-145.
          pubmed: 2017613
        22. Hines MT, Paasch KM, Alperin DC, Palmer GH, Westhoff NC, Hines SA. Immunity to Rhodococcus equi: antigen-specific recall responses in the lungs of adult horses. Vet. Immunol. Immunopathol. 79:101-114.
          pubmed: 11356253
        23. Hines SA, Stone DM, Hines MT, Alperin DC, Knowles DP, Norton LK, Hamilton MJ, Davis WC, McGuire TC. Clearance of virulent but not avirulent Rhodococcus equi from the lungs of adult horses is associated with intracytoplasmic gamma interferon production by CD4+ and CD8+ T lymphocytes. Clin. Diagn. Lab. Immunol. 10:210-215.
          pmc: PMC150533pubmed: 12626444
        24. Hooper-McGrevy KE, Giguère S, Wilkie BN, Prescott JF. Evaluation of equine immunoglobulin specific for Rhodococcus equi virulence-associated proteins A and C for use in protecting foals against Rhodococcus equi-induced pneumonia. Am. J. Vet. Res. 62:1307-1313.
          pubmed: 11497456
        25. Hooper-McGrevy KE, Wilkie BN, Prescott JF. Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins. Clin. Diagn. Lab. Immunol. 10:345-351.
          pmc: PMC154967pubmed: 12738629
        26. Hooper-McGrevy KE, Wilkie BN, Prescott JF. Virulence-associated protein-specific serum immunoglobulin G-isotype expression in young foals protected against Rhodococcus equi pneumonia by oral immunization with virulent R. equi. Vaccine 23:5760-5767.
          pubmed: 16112256
        27. Horowitz ML, Cohen ND, Takai S, Becu T, Chaffin MK, Chu KK, Magdesian KG, Martens RJ. Application of Sartwell's model (lognormal distribution of incubation periods) to age at onset and age at death of foals with Rhodococcus equi pneumonia as evidence of perinatal infection. J. Vet. Intern. Med. 15:171-175.
          pubmed: 11380023
        28. Johnson JA, Prescott JF, Markham RJ. The pathology of experimental Corynebacterium equi infection in foals following intrabronchial challenge. Vet. Pathol. 20:440-449.
          pubmed: 6623848
        29. Kanaly ST, Hines SA, Palmer GH. Cytokine modulation alters pulmonary clearance of Rhodococcus equi and development of granulomatous pneumonia. Infect. Immun. 63:3037-3041.
          pmc: PMC173413pubmed: 7622227
        30. Kanaly ST, Hines SA, Palmer GH. Transfer of a CD4+ Th1 cell line to nude mice effects clearance of Rhodococcus equi from the lung. Infect. Immun. 64:1126-1132.
          pmc: PMC173893pubmed: 8606068
        31. Lopez AM, Hines MT, Palmer GH, Alperin DC, Hines SA. Identification of pulmonary T-lymphocyte and serum antibody isotype responses associated with protection against Rhodococcus equi. Clin. Diagn. Lab. Immunol. 9:1270-1276.
          pmc: PMC130094pubmed: 12414760
        32. Marchant A, Goetghebuer T, Ota MO, Wolfe I, Ceesay SJ, De Groote D, Corrah T, Bennett S, Wheeler J, Huygen K, Aaby P, McAdam KP, Newport MJ. Newborns develop a Th1-type immune response to Mycobacterium bovis bacillus Calmette-Guerin vaccination. J. Immunol. 163:2249-2255.
          pubmed: 10438968
        33. Martens RJ, Martens JG, Fiske RA. Rhodococcus equi foal pneumonia: pathogenesis and immunoprophylaxis. Proc. Am. Assoc. Equine Pract. 35:189-213.
        34. Martinez X, Brandt C, Saddallah F, Tougne C, Barrios C, Wild F, Dougan G, Lambert PH, Siegrist CA. DNA immunization circumvents deficient induction of T helper type 1 and cytotoxic T lymphocyte responses in neonates and during early life. Proc. Natl. Acad. Sci. USA 94:8726-8731.
          pmc: PMC23100pubmed: 9238045
        35. Ota MO, Vekemans J, Schlegel-Haueter SE, Fielding K, Sanneh M, Kidd M, Newport MJ, Aaby P, Whittle H, Lambert PH, McAdam KP, Siegrist CA, Marchant A. Influence of Mycobacterium bovis bacillus Calmette-Guerin on antibody and cytokine responses to human neonatal vaccination. J. Immunol. 168:919-925.
          pubmed: 11777990
        36. Patton KM, McGuire TC, Fraser DG, Hines SA. Rhodococcus equi-infected macrophages are recognized and killed by CD8+ T lymphocytes in a major histocompatibility complex class I-unrestricted fashion. Infect. Immun. 72:7073-7083.
          pmc: PMC529141pubmed: 15557631
        37. Pongracz J, Parnell S, Anderson G, Jaffrezou JP, Jenkinson E. Con A activates an Akt/PKB dependent survival mechanism to modulate TCR induced cell death in double positive thymocytes. Mol. Immunol. 39:1013-1023.
          pubmed: 12749908
        38. Sarzotti M, Robbins DS, Hoffman PM. Induction of protective CTL responses in newborn mice by a murine retrovirus. Science 271:1726-1728.
          pubmed: 8596933
        39. Sheoran AS, Timoney JF, Holmes MA, Karzenski SS, Crisman MV. Immunoglobulin isotypes in sera and nasal mucosal secretions and their neonatal transfer and distribution in horses. Am. J. Vet. Res. 61:1099-1105.
          pubmed: 10976743
        40. Takai S, Anzai T, Yamaguchi K, Kakizaki S, Takahagi J, Sato Y, Takehara F, Tamada Y, Matsukura S, Tani A, Kato M, Seno N, Sasaki Y, Tsubaki S, Kamada M. Prevalence of virulence plasmids in environmental isolates of Rhodococcus equi from horse-breeding farms in Hokkaido. J. Equine Sci. 5:21-25.
        41. Takai S, Fukunaga N, Ochiai S, Sakai T, Sasaki Y, Tsubaki S. Isolation of virulent and intermediately virulent Rhodococcus equi from soil and sand on parks and yards in Japan. J. Vet. Med. Sci. 58:669-672.
          pubmed: 8844605
        42. Takai S, Hines SA, Sekizaki T, Nicholson VM, Alperin DA, Osaki M, Takamatsu D, Nakamura M, Suzuki K, Ogino N, Kakuda T, Dan H, Prescott JF. DNA Sequence and comparison of virulence plasmids from Rhodococcus equi ATCC 33701 and 103. Infect. Immun. 68:6840-6847.
          pmc: PMC97788pubmed: 11083803
        43. Takai S, Nakata I, Fukii N, Kimura Y, Sasaki Y, Kakuda T, Tsubaki S, Kondo T, Sugiura T. Isotype-specific antibody responses to Rhodococcus equi in foals on a horse-breeding farm with a persistent incidence of R. equi infection. J. Equine Sci. 13:63-70.
        44. Vekemans J, Amedei A, Ota MO, D'Elios MM, Goetghebuer T, Ismaili J, Newport MJ, Del Prete G, Goldman M, McAdam KP, Marchant A. Neonatal bacillus Calmette-Guerin vaccination induces adult-like IFN-gamma production by CD4+ T lymphocytes. Eur. J. Immunol. 31:1531-1535.
          pubmed: 11465110
        45. Wada R, Kamada M, Anzai T, Nakanishi A, Kanemaru T, Takai S, Tsubaki S. Pathogenicity and virulence of Rhodococcus equi in foals following intratracheal challenge. Vet. Microbiol. 56:301-312.
          pubmed: 9226844
        46. Wagner B. Immunoglobulins and immunoglobulin genes of the horse. Dev. Comp. Immunol. 30:155-164.
          pubmed: 16046236
        47. Wagner B, Miller DC, Lear TL, Antczak DF. The complete map of the Ig heavy chain constant gene region reveals evidence for seven IgG isotypes and for IgD in the horse. J. Immunol. 173:3230-3242.
          pubmed: 15322185
        48. Wagner B, Miller WH, Morgan EE, Hillegas JM, Erb HN, Leibold W, Antczak DF. IgE and IgG antibodies in skin allergy of the horse. Vet. Res. 37:813-825.
          pubmed: 16973120
        49. Witonsky S, Gogal RM Jr, Buechner-Maxwell V, Ahmed SA. Immunologic analysis of blood samples obtained from horses and stored for twenty-four hours. Am. J. Vet. Res. 64:1003-1009.
          pubmed: 12926593
        50. Zink MC, Johnson JA. Cellular constituents of clinically normal foal bronchoalveolar lavage fluid during postnatal maturation. Am. J. Vet. Res. 45:893-897.
          pubmed: 6732021

        Citations

        This article has been cited 15 times.
        1. Berghaus LJ, Venner M, Helbig H, Hildebrandt D, Hart K. The potential value of cytokine, cortisol and vitamin D profiles in foals from birth to weaning for respiratory disease prediction on a farm endemic for Rhodococcus equi pneumonia. Equine Vet J 2026 Mar;58(2):359-371.
          doi: 10.1111/evj.70093pubmed: 40923138google scholar: lookup
        2. Mizuguchi Y, Tsuzuki N, Ebana MD, Suzuki Y, Kakuda T. IgG Subtype Response against Virulence-Associated Protein A in Foals Naturally Infected with Rhodococcus equi. Vet Sci 2024 Sep 9;11(9).
          doi: 10.3390/vetsci11090422pubmed: 39330801google scholar: lookup
        3. da Silveira BP, Cohen ND, Lawhon SD, Watson RO, Bordin AI. Protective immune response against Rhodococcus equi: An innate immunity-focused review. Equine Vet J 2025 May;57(3):563-586.
          doi: 10.1111/evj.14214pubmed: 39258739google scholar: lookup
        4. Anna M, Łukasz M, Adam O, Chełmońska-Soyta A. Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life. Sci Rep 2022 Sep 22;12(1):15795.
          doi: 10.1038/s41598-022-17532-1pubmed: 36138050google scholar: lookup
        5. Rose RS, Richardson KH, Latvanen EJ, Hanson CA, Resmini M, Sanders IA. Microbial Degradation of Plastic in Aqueous Solutions Demonstrated by CO(2) Evolution and Quantification. Int J Mol Sci 2020 Feb 11;21(4).
          doi: 10.3390/ijms21041176pubmed: 32053975google scholar: lookup
        6. Tallmadge RL, Miller SC, Parry SA, Felippe MJB. Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate. PLoS One 2017;12(5):e0177831.
          doi: 10.1371/journal.pone.0177831pubmed: 28520789google scholar: lookup
        7. Rocha JN, Cohen ND, Bordin AI, Brake CN, Giguère S, Coleman MC, Alaniz RC, Lawhon SD, Mwangi W, Pillai SD. Oral Administration of Electron-Beam Inactivated Rhodococcus equi Failed to Protect Foals against Intrabronchial Infection with Live, Virulent R. equi. PLoS One 2016;11(2):e0148111.
          doi: 10.1371/journal.pone.0148111pubmed: 26828865google scholar: lookup
        8. Hamza E, Mirkovitch J, Steinbach F, Marti E. Regulatory T cells in early life: comparative study of CD4+CD25high T cells from foals and adult horses. PLoS One 2015;10(3):e0120661.
          doi: 10.1371/journal.pone.0120661pubmed: 25790481google scholar: lookup
        9. Vendrig JC, Coffeng LE, Fink-Gremmels J. Effects of Separate and Concomitant TLR-2 and TLR-4 Activation in Peripheral Blood Mononuclear Cells of Newborn and Adult Horses. PLoS One 2013;8(6):e66897.
          doi: 10.1371/journal.pone.0066897pubmed: 23840549google scholar: lookup
        10. Kachroo P, Ivanov I, Seabury AG, Liu M, Chowdhary BP, Cohen ND. Age-related changes following in vitro stimulation with Rhodococcus equi of peripheral blood leukocytes from neonatal foals. PLoS One 2013;8(5):e62879.
          doi: 10.1371/journal.pone.0062879pubmed: 23690962google scholar: lookup
        11. Harris SP, Hines MT, Mealey RH, Alperin DC, Hines SA. Early development of cytotoxic T lymphocytes in neonatal foals following oral inoculation with Rhodococcus equi. Vet Immunol Immunopathol 2011 Jun 15;141(3-4):312-6.
          doi: 10.1016/j.vetimm.2011.03.015pubmed: 21481947google scholar: lookup
        12. Ryan C, Giguère S. Equine neonates have attenuated humoral and cell-mediated immune responses to a killed adjuvanted vaccine compared to adult horses. Clin Vaccine Immunol 2010 Dec;17(12):1896-902.
          doi: 10.1128/CVI.00328-10pubmed: 20943883google scholar: lookup
        13. Wagner B, Burton A, Ainsworth D. Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory TR1 cell activation and a delay of the Th2 cell response in equine neonates and foals. Vet Res 2010 Jul-Aug;41(4):47.
          doi: 10.1051/vetres/2010019pubmed: 20374696google scholar: lookup
        14. Muscatello G, Gilkerson JR, Browning GF. Detection of virulent Rhodococcus equi in exhaled air samples from naturally infected foals. J Clin Microbiol 2009 Mar;47(3):734-7.
          doi: 10.1128/JCM.01395-08pubmed: 19144811google scholar: lookup
        15. Flaminio MJ, Nydam DV, Marquis H, Matychak MB, Giguère S. Foal monocyte-derived dendritic cells become activated upon Rhodococcus equi infection. Clin Vaccine Immunol 2009 Feb;16(2):176-83.
          doi: 10.1128/CVI.00336-08pubmed: 19109450google scholar: lookup
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