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Journal of veterinary internal medicine2022; 36(6); 2213-2223; doi: 10.1111/jvim.16562

Fecal microbiota of horses with colitis and its association with laminitis and survival during hospitalization.

Abstract: The association of microbiota with clinical outcomes and the taxa associated with colitis in horses remains generally unknown. Objective: Describe the fecal microbiota of horses with colitis and investigate the association of the fecal microbiota with the development of laminitis and survival. Methods: Thirty-six healthy and 55 colitis horses subdivided into laminitis (n = 15) and non-laminitis (n = 39, 1 horse with chronic laminitis was removed from this comparison) and survivors (n = 27) and nonsurvivors (n = 28). Methods: Unmatched case-control study. The Illumina MiSeq platform targeting the V4 region of the 16S ribosomal RNA gene was used to assess the microbiota. Results: The community membership (Jaccard index) and structure (Yue and Clayton index) were different (analysis of molecular variance [AMOVA]; P < .001) between healthy and colitis horses. The linear discriminant analysis effect size (LEfSe; linear discriminant analysis [LDA] >3; P < .05) and random forest analyses found Enterobacteriaceae, Lactobacillus, Streptococcus, and Enterococcus enriched in colitis horses, whereas Treponema, Faecalibacterium, Ruminococcaceae, and Lachnospiraceae were enriched in healthy horses. The community membership and structure of colitis horses with or without laminitis was (AMOVA; P > .05). Enterobacteriaceae, Streptococcus, and Lactobacillus were enriched in horses with laminitis (LDA > 3; P < .05). The community membership (AMOVA; P = .008) of surviving and nonsurviving horses was different. Nonsurviving horses had an enrichment of Enterobacteriaceae, Pseudomonas, Streptococcus, and Enterococcus (LDA >3; P < .05). Conclusions: Differences in the microbiota of horses with colitis that survive or do not survive are minor and, similarly, the microbiota differences in horses with colitis that do or do not develop laminitis are minor.
© 2022 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2022-10-21 PubMed ID: 36271677PubMed Central: PMC9708523DOI: 10.1111/jvim.16562Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study aims to understand the relationship between the fecal microbiota of horses suffering from colitis and the occurrence of laminitis and survival rate. It uncovers minor differences in the microbiota of horses with colitis that either survive or succumb to the illness, and in those that develop laminitis or not.

Study Design and Methodology

  • The researchers studied the microbiota in 36 healthy horses and 55 horses with colitis, further subdividing the colitis set into 15 horses with laminitis and 39 without. They excluded a horse suffering from chronic laminitis from the comparison.
  • This work was an unmatched case-control study, where the researchers compared different groups exhibiting different outcomes (colitis with or without laminitis, survival or non-survival), with a healthy control group.
  • The technology used was an Illumina MiSeq platform, targeting the V4 region of the 16S ribosomal RNA gene for the assessment of microbiota. This technology enables a comprehensive analysis of microbial communities within the gut.

Primary Findings

  • The study found a significant difference in the community membership (composition of bacterial species) and structure (relative abundance of bacterial taxa) between healthy and colitis-affected horses.
  • Specific families of bacteria – Enterobacteriaceae, Lactobacillus, Streptococcus, and Enterococcus – were more frequently found in horses with colitis. Conversely, Treponema, Faecalibacterium, Ruminococcaceae, and Lachnospiraceae were abundant in healthy horses.
  • The community membership and structure of horses with laminitis did not significantly differ from those without.
  • A variation in the microbial community was found between surviving and nonsurviving horses. More specifically, the nonsurvivors had a higher abundance of Enterobacteriaceae, Pseudomonas, Streptococcus, and Enterococcus.

Conclusion

  • The researchers observed minimal differences in the microbiota of horses with colitis based on whether they survived the condition or not and whether they developed laminitis or not.
  • The study is of importance for vets and researchers as it implicates certain bacterial taxa in horses’ health conditions, suggesting potential targets for future therapeutic interventions.

Cite This Article

APA
Ayoub C, Arroyo LG, MacNicol JL, Renaud D, Weese JS, Gomez DE. (2022). Fecal microbiota of horses with colitis and its association with laminitis and survival during hospitalization. J Vet Intern Med, 36(6), 2213-2223. https://doi.org/10.1111/jvim.16562

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 36
Issue: 6
Pages: 2213-2223

Researcher Affiliations

Ayoub, Cosette
  • Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Arroyo, Luis G
  • Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
MacNicol, Jennifer L
  • Department of Animal Biosciences, Ontario Agricultural College, University of Guelph, Guelph, Ontario, Canada.
Renaud, David
  • Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Weese, J Scott
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Gomez, Diego E
  • Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.

MeSH Terms

  • Horses
  • Animals
  • Case-Control Studies
  • Feces / microbiology
  • Microbiota / genetics
  • Colitis / veterinary
  • Streptococcus
  • Hospitalization
  • Horse Diseases / microbiology

Grant Funding

  • 055097 / Equine Guelph

Conflict of Interest Statement

Authors declare no conflict of interest.

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