FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Veterinary research communications2011; 35(6); 355-365; doi: 10.1007/s11259-011-9480-z

Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells.

Abstract: Adipose tissue-derived stem cells (ADSCs) represent a promising subpopulation of adult stem cells for tissue engineering applications in veterinary medicine. In this study we focused on the morphological and molecular biological properties of the ADSCs. The expression of stem cell markers Oct4, Nanog and the surface markers CD90 and CD105 were detected using RT-PCR. ADSCs showed a proliferative potential and were capable of adipogenic and osteogenic differentiation. Expression of Alkaline phosphatase (AP), phosphoprotein (SPP1), Runx2 and osteocalcin (OC) mRNA were positive in osteogenic lineages and peroxisome proliferator activated receptor (Pparγ2) mRNA was positive in adipogenic lineages. ADSCs show stem cell and surface marker profiles and differentiation characteristics that are similar to but distinct from other adult stem cells, such as bone marrow-derived mesenchymal stem cells (BM-MSCs). The availability of an easily accessible and reproducible cell source may greatly facilitate the development of stem cell based tissue engineering and therapies for regenerative equine medicine.
Get Full Text
Publication Date: 2011-05-26 PubMed ID: 21614641DOI: 10.1007/s11259-011-9480-zGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study delves into the properties of Adipose tissue-derived stem cells (ADSCs) in horses, focusing on their potential for tissue engineering applications in veterinary medicine. It examines the cells’ morphological features, molecular biological properties, and their differentiation characteristics, drawing comparisons with other adult stem cells.

Research study focus and overview

  • The research aims to better understand and characterize equine adipose tissue-derived mesenchymal stem cells (ADSCs), an especially promising type of adult stem cells for tissue engineering in veterinary medicine.
  • Emphasis is put on studying the morphological and molecular biological properties of ADSCs, including their proliferation potential and differentiation into types of cells that form adipose (fat) and bone tissues.

Key Findings

  • The research discovered that ADSCs express stem cell markers like Oct4 and Nanog, and surface markers like CD90 and CD105. This is significant as these markers are typically used to identify and distinguish stem cells.
  • ADSCs have a significant proliferative potential, which means they can create multiple cell generations over time. This is a critical characteristic for stem cells to be effective in regenerative therapies and tissue engineering.
  • The ADSCs also demonstrated their capability to undergo adipogenic and osteogenic differentiation; which means they can specialize into adipose (fat) or bone cells. This ability confirms these cells’ versatility and potential efficacy in tissue regeneration.
  • The researchers also detected the expression of various types of mRNA in cells undergoing differentiation. These included Alkaline phosphatase (AP), phosphoprotein (SPP1), Runx2 and osteocalcin (OC) in osteogenic lineages; and peroxisome proliferator activated receptor (Pparγ2) in adipogenic lineages.
  • It was found that ADSCs have stem cell and surface marker profiles, and differentiation characteristics that are similar but still distinct from other adult stem cells, like bone marrow-derived mesenchymal stem cells (BM-MSCs).

Implications and Conclusions

  • This study provides important insights into the properties of equine ADSCs, which could greatly benefit the development of stem cell-based tissue engineering and regenerative therapies in veterinary medicine.
  • Furthermore, acknowledging that adipose tissue is an easily accessible and reproducible cell source, the use of ADSCs could simplify and facilitate various treatment processes.
  • The findings also highlight the pivotal role that molecular biology techniques, such as RT-PCR, play in identifying and characterizing stem cells and their properties.

Cite This Article

APA
Raabe O, Shell K, Würtz A, Reich CM, Wenisch S, Arnhold S. (2011). Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells. Vet Res Commun, 35(6), 355-365. https://doi.org/10.1007/s11259-011-9480-z

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 35
Issue: 6
Pages: 355-365

Researcher Affiliations

Raabe, Oksana
  • Institute of Veterinary -Anatomy, -Histology and -Embryology, Justus-Liebig University of Giessen, Frankfurterstrasse, Germany, oksana.raabe@vetmed.uni-giessen.de
Shell, Katja
    Würtz, Antonia
      Reich, Christine Maria
        Wenisch, Sabine
          Arnhold, Stefan

            MeSH Terms

            • Adipose Tissue / cytology
            • Adipose Tissue / metabolism
            • Adult Stem Cells / cytology
            • Adult Stem Cells / metabolism
            • Alkaline Phosphatase / metabolism
            • Animals
            • Antigens, CD / metabolism
            • Biomarkers / metabolism
            • Cell Differentiation
            • Cells, Cultured
            • Core Binding Factor Alpha 1 Subunit / metabolism
            • Cytokines / metabolism
            • DNA-Binding Proteins / metabolism
            • Horses / physiology
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / metabolism
            • Osteocalcin / metabolism
            • PPAR gamma / metabolism
            • RNA, Messenger / genetics
            • RNA, Messenger / metabolism
            • Reverse Transcriptase Polymerase Chain Reaction
            • Tissue Engineering / methods
            • Tissue Engineering / veterinary

            References

            This article includes 33 references
            1. de Mattos Carvalho A, Alves AL, Golim MA, Moroz A, Hussni CA, de Oliveira PG, Deffune E. Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.. Vet Immunol Immunopathol 2009 Dec 15;132(2-4):303-6.
              pubmed: 19647331doi: 10.1016/j.vetimm.2009.06.014google scholar: lookup
            2. Colleoni S, Bottani E, Tessaro I, Mari G, Merlo B, Romagnoli N, Spadari A, Galli C, Lazzari G. Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor.. Vet Res Commun 2009 Dec;33(8):811-21.
              pubmed: 19472068doi: 10.1007/s11259-009-9229-0google scholar: lookup
            3. Latchman DS. Transcription factors: an overview.. Int J Biochem Cell Biol 1997 Dec;29(12):1305-12.
              pubmed: 9570129doi: 10.1016/s1357-2725(97)00085-xgoogle scholar: lookup
            4. Martinello T, Bronzini I, Maccatrozzo L, Iacopetti I, Sampaolesi M, Mascarello F, Patruno M. Cryopreservation does not affect the stem characteristics of multipotent cells isolated from equine peripheral blood.. Tissue Eng Part C Methods 2010 Aug;16(4):771-81.
              pubmed: 19839741doi: 10.1089/ten.TEC.2009.0512google scholar: lookup
            5. Raabe O, Reich C, Wenisch S, Hild A, Burg-Roderfeld M, Siebert HC, Arnhold S. Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells.. Histochem Cell Biol 2010 Dec;134(6):545-54.
              pubmed: 21076963doi: 10.1007/s00418-010-0760-4google scholar: lookup
            6. Tontonoz P, Hu E, Graves RA, Budavari AI, Spiegelman BM. mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer.. Genes Dev 1994 May 15;8(10):1224-34.
              pubmed: 7926726doi: 10.1101/gad.8.10.1224google scholar: lookup
            7. Vidal MA, Kilroy GE, Lopez MJ, Johnson JR, Moore RM, Gimble JM. Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells.. Vet Surg 2007 Oct;36(7):613-22.
              pubmed: 17894587doi: 10.1111/j.1532-950X.2007.00313.xgoogle scholar: lookup
            8. De Schauwer C, Meyer E, Van de Walle GR, Van Soom A. Markers of stemness in equine mesenchymal stem cells: a plea for uniformity.. Theriogenology 2011 May;75(8):1431-43.
              pubmed: 21196039doi: 10.1016/j.theriogenology.2010.11.008google scholar: lookup
            9. Cremonesi F, Violini S, Lange Consiglio A, Ramelli P, Ranzenigo G, Mariani P. Isolation, in vitro culture and characterization of foal umbilical cord stem cells at birth.. Vet Res Commun 2008 Sep;32 Suppl 1:S139-42.
              pubmed: 18688745doi: 10.1007/s11259-008-9116-0google scholar: lookup
            10. Arrigoni E, Lopa S, de Girolamo L, Stanco D, Brini AT. Isolation, characterization and osteogenic differentiation of adipose-derived stem cells: from small to large animal models.. Cell Tissue Res 2009 Dec;338(3):401-11.
              pubmed: 19882172doi: 10.1007/s00441-009-0883-xgoogle scholar: lookup
            11. Hattori H, Sato M, Masuoka K, Ishihara M, Kikuchi T, Matsui T, Takase B, Ishizuka T, Kikuchi M, Fujikawa K, Ishihara M. Osteogenic potential of human adipose tissue-derived stromal cells as an alternative stem cell source.. Cells Tissues Organs 2004;178(1):2-12.
              pubmed: 15550755doi: 10.1159/000081088google scholar: lookup
            12. Violini S, Ramelli P, Pisani LF, Gorni C, Mariani P. Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12.. BMC Cell Biol 2009 Apr 22;10:29.
              pubmed: 19383177doi: 10.1186/1471-2121-10-29google scholar: lookup
            13. Neupane M, Chang CC, Kiupel M, Yuzbasiyan-Gurkan V. Isolation and characterization of canine adipose-derived mesenchymal stem cells.. Tissue Eng Part A 2008 Jun;14(6):1007-15.
              pubmed: 19230125doi: 10.1089/ten.tea.2007.0207google scholar: lookup
            14. Mambelli LI, Santos EJ, Frazão PJ, Chaparro MB, Kerkis A, Zoppa AL, Kerkis I. Characterization of equine adipose tissue-derived progenitor cells before and after cryopreservation.. Tissue Eng Part C Methods 2009 Mar;15(1):87-94.
              pubmed: 19196122doi: 10.1089/ten.tec.2008.0186google scholar: lookup
            15. Mimeault M, Batra SK. Recent progress on tissue-resident adult stem cell biology and their therapeutic implications.. Stem Cell Rev 2008 Spring;4(1):27-49.
              pubmed: 18288619doi: 10.1007/s12015-008-9008-2google scholar: lookup
            16. Carrade DD, Owens SD, Galuppo LD, Vidal MA, Ferraro GL, Librach F, Buerchler S, Friedman MS, Walker NJ, Borjesson DL. Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.. Cytotherapy 2011 Apr;13(4):419-30.
              pubmed: 21105841doi: 10.3109/14653249.2010.536213google scholar: lookup
            17. Reed SA, Johnson SE. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.. J Cell Physiol 2008 May;215(2):329-36.
              pubmed: 17929245doi: 10.1002/jcp.21312google scholar: lookup
            18. Martinello T, Bronzini I, Maccatrozzo L, Mollo A, Sampaolesi M, Mascarello F, Decaminada M, Patruno M. Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation.. Res Vet Sci 2011 Aug;91(1):18-24.
              pubmed: 20732703doi: 10.1016/j.rvsc.2010.07.024google scholar: lookup
            19. Ribitsch I, Burk J, Delling U, Geißler C, Gittel C, Jülke H, Brehm W. Basic science and clinical application of stem cells in veterinary medicine.. Adv Biochem Eng Biotechnol 2010;123:219-63.
              pubmed: 20309674doi: 10.1007/10_2010_66google scholar: lookup
            20. Hoynowski SM, Fry MM, Gardner BM, Leming MT, Tucker JR, Black L, Sand T, Mitchell KE. Characterization and differentiation of equine umbilical cord-derived matrix cells.. Biochem Biophys Res Commun 2007 Oct 19;362(2):347-53.
              pubmed: 17719011doi: 10.1016/j.bbrc.2007.07.182google scholar: lookup
            21. Nixon AJ, Dahlgren LA, Haupt JL, Yeager AE, Ward DL. Effect of adipose-derived nucleated cell fractions on tendon repair in horses with collagenase-induced tendinitis.. Am J Vet Res 2008 Jul;69(7):928-37.
              pubmed: 18593247doi: 10.2460/ajvr.69.7.928google scholar: lookup
            22. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose tissue: implications for cell-based therapies.. Tissue Eng 2001 Apr;7(2):211-28.
              pubmed: 11304456doi: 10.1089/107632701300062859google scholar: lookup
            23. Lee RH, Kim B, Choi I, Kim H, Choi HS, Suh K, Bae YC, Jung JS. Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue.. Cell Physiol Biochem 2004;14(4-6):311-24.
              pubmed: 15319535doi: 10.1159/000080341google scholar: lookup
            24. Rebelatto CK, Aguiar AM, Moretão MP, Senegaglia AC, Hansen P, Barchiki F, Oliveira J, Martins J, Kuligovski C, Mansur F, Christofis A, Amaral VF, Brofman PS, Goldenberg S, Nakao LS, Correa A. Dissimilar differentiation of mesenchymal stem cells from bone marrow, umbilical cord blood, and adipose tissue.. Exp Biol Med (Maywood) 2008 Jul;233(7):901-13.
              pubmed: 18445775doi: 10.3181/0712-RM-356google scholar: lookup
            25. Richardson LE, Dudhia J, Clegg PD, Smith R. Stem cells in veterinary medicine--attempts at regenerating equine tendon after injury.. Trends Biotechnol 2007 Sep;25(9):409-16.
              pubmed: 17692415doi: 10.1016/j.tibtech.2007.07.009google scholar: lookup
            26. Del Bue M, Riccò S, Ramoni R, Conti V, Gnudi G, Grolli S. Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo.. Vet Res Commun 2008 Sep;32 Suppl 1:S51-5.
              pubmed: 18683070doi: 10.1007/s11259-008-9093-3google scholar: lookup
            27. Kisiday JD, Kopesky PW, Evans CH, Grodzinsky AJ, McIlwraith CW, Frisbie DD. Evaluation of adult equine bone marrow- and adipose-derived progenitor cell chondrogenesis in hydrogel cultures.. J Orthop Res 2008 Mar;26(3):322-31.
              pubmed: 17960654doi: 10.1002/jor.20508google scholar: lookup
            28. Dragoo JL, Lieberman JR, Lee RS, Deugarte DA, Lee Y, Zuk PA, Hedrick MH, Benhaim P. Tissue-engineered bone from BMP-2-transduced stem cells derived from human fat.. Plast Reconstr Surg 2005 May;115(6):1665-73.
              pubmed: 15861072doi: 10.1097/01.prs.0000161459.90856.abgoogle scholar: lookup
            29. Lin Y, Liu L, Li Z, Qiao J, Wu L, Tang W, Zheng X, Chen X, Yan Z, Tian W. Pluripotency potential of human adipose-derived stem cells marked with exogenous green fluorescent protein.. Mol Cell Biochem 2006 Oct;291(1-2):1-10.
              pubmed: 16718363doi: 10.1007/s11010-006-9188-5google scholar: lookup
            30. Arnhold SJ, Goletz I, Klein H, Stumpf G, Beluche LA, Rohde C, Addicks K, Litzke LF. Isolation and characterization of bone marrow-derived equine mesenchymal stem cells.. Am J Vet Res 2007 Oct;68(10):1095-105.
              pubmed: 17916017doi: 10.2460/ajvr.68.10.1095google scholar: lookup
            31. Vieira NM, Brandalise V, Zucconi E, Secco M, Strauss BE, Zatz M. Isolation, characterization, and differentiation potential of canine adipose-derived stem cells.. Cell Transplant 2010;19(3):279-89.
              pubmed: 19995482doi: 10.3727/096368909X481764google scholar: lookup
            32. da Silva Meirelles L, Chagastelles PC, Nardi NB. Mesenchymal stem cells reside in virtually all post-natal organs and tissues.. J Cell Sci 2006 Jun 1;119(Pt 11):2204-13.
              pubmed: 16684817doi: 10.1242/jcs.02932google scholar: lookup
            33. Torres FC, Rodrigues CJ, Stocchero IN, Ferreira MC. Stem cells from the fat tissue of rabbits: an easy-to-find experimental source.. Aesthetic Plast Surg 2007 Sep-Oct;31(5):574-8.
              pubmed: 17576503doi: 10.1007/s00266-007-0001-ygoogle scholar: lookup

            Citations

            This article has been cited 15 times.
            1. Petrova V, Yonkova P, Simeonova G, Vachkova E. Horse serum potentiates cellular viability and improves indomethacin-induced adipogenesis in equine subcutaneous adipose-derived stem cells (ASCs). Int J Vet Sci Med 2023;11(1):94-105.
              doi: 10.1080/23144599.2023.2248805pubmed: 37655053google scholar: lookup
            2. Elashry MI, Baulig N, Wagner AS, Klymiuk MC, Kruppke B, Hanke T, Wenisch S, Arnhold S. Combined macromolecule biomaterials together with fluid shear stress promote the osteogenic differentiation capacity of equine adipose-derived mesenchymal stem cells. Stem Cell Res Ther 2021 Feb 12;12(1):116.
              doi: 10.1186/s13287-021-02146-7pubmed: 33579348google scholar: lookup
            3. Arnhold S, Elashry MI, Klymiuk MC, Geburek F. Investigation of stemness and multipotency of equine adipose-derived mesenchymal stem cells (ASCs) from different fat sources in comparison with lipoma. Stem Cell Res Ther 2019 Oct 22;10(1):309.
              doi: 10.1186/s13287-019-1429-0pubmed: 31640774google scholar: lookup
            4. Metcalf GL, McClure SR, Hostetter JM, Martinez RF, Wang C. Evaluation of adipose-derived stromal vascular fraction from the lateral tailhead, inguinal region, and mesentery of horses. Can J Vet Res 2016 Oct;80(4):294-301.
              pubmed: 27733784
            5. Ock SA, Baregundi Subbarao R, Lee YM, Lee JH, Jeon RH, Lee SL, Park JK, Hwang SC, Rho GJ. Comparison of Immunomodulation Properties of Porcine Mesenchymal Stromal/Stem Cells Derived from the Bone Marrow, Adipose Tissue, and Dermal Skin Tissue. Stem Cells Int 2016;2016:9581350.
              doi: 10.1155/2016/9581350pubmed: 26798368google scholar: lookup
            6. Arnhold S, Wenisch S. Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine. Am J Stem Cells 2015;4(1):1-12.
              pubmed: 25973326
            7. Tessier L, Bienzle D, Williams LB, Koch TG. Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells. PLoS One 2015;10(4):e0122954.
              doi: 10.1371/journal.pone.0122954pubmed: 25902064google scholar: lookup
            8. Gittel C, Brehm W, Burk J, Juelke H, Staszyk C, Ribitsch I. Isolation of equine multipotent mesenchymal stromal cells by enzymatic tissue digestion or explant technique: comparison of cellular properties. BMC Vet Res 2013 Oct 29;9:221.
              doi: 10.1186/1746-6148-9-221pubmed: 24168625google scholar: lookup
            9. Raabe O, Shell K, Goessl A, Crispens C, Delhasse Y, Eva A, Scheiner-Bobis G, Wenisch S, Arnhold S. Effect of extracorporeal shock wave on proliferation and differentiation of equine adipose tissue-derived mesenchymal stem cells in vitro. Am J Stem Cells 2013;2(1):62-73.
              pubmed: 23671817
            10. Ranera B, Remacha AR, Álvarez-Arguedas S, Romero A, Vázquez FJ, Zaragoza P, Martín-Burriel I, Rodellar C. Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue. BMC Vet Res 2012 Aug 22;8:142.
              doi: 10.1186/1746-6148-8-142pubmed: 22913590google scholar: lookup
            11. Reich CM, Raabe O, Wenisch S, Bridger PS, Kramer M, Arnhold S. Isolation, culture and chondrogenic differentiation of canine adipose tissue- and bone marrow-derived mesenchymal stem cells--a comparative study. Vet Res Commun 2012 Jun;36(2):139-48.
              doi: 10.1007/s11259-012-9523-0pubmed: 22392598google scholar: lookup
            12. Klymiuk MC, Balz N, Elashry MI, Wenisch S, Arnhold S. Effect of storage conditions on the quality of equine and canine mesenchymal stem cell derived nanoparticles including extracellular vesicles for research and therapy. Discov Nano 2024 May 3;19(1):80.
              doi: 10.1186/s11671-024-04026-4pubmed: 38700790google scholar: lookup
            13. Ivanova Z, Petrova V, Grigorova N, Vachkova E. Identification of the Reference Genes for Relative qRT-PCR Assay in Two Experimental Models of Rabbit and Horse Subcutaneous ASCs. Int J Mol Sci 2024 Feb 14;25(4).
              doi: 10.3390/ijms25042292pubmed: 38396967google scholar: lookup
            14. Ferreira-Baptista C, Ferreira R, Fernandes MH, Gomes PS, Colaço B. Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells' Biological Profile and Osteogenic Potential in Companion Animals. Vet Sci 2023 Nov 24;10(12).
              doi: 10.3390/vetsci10120673pubmed: 38133224google scholar: lookup
            15. Li H, Xiong S, Masieri FF, Monika S, Lethaus B, Savkovic V. Mesenchymal Stem Cells Isolated from Equine Hair Follicles Using a Method of Air-Liquid Interface. Stem Cell Rev Rep 2023 Nov;19(8):2943-2956.
              doi: 10.1007/s12015-023-10619-wpubmed: 37733199google scholar: lookup
            Subscribe to our newsletter
            Join 99,727 horse owners like you who receive our email updates on horse health and nutrition.