Genetic analysis of Streptococcus equi subsp. equi isolated from horses imported into Japan.
Abstract: Strangles is a commonly diagnosed and important infectious disease of equids worldwide, caused by Streptococcus equi subsp. equi. We determined the SeM genotypes of S. equi isolated from imported horses at the Japanese border within the past 8 years, which allowed us to classify 12 strains isolated from these horses from each exporter into four allelic groups. These alleles were different from the alleles of past isolates found in Japan. Furthermore, four strains classified into the same allele were isolated from horses from one exporter over several years. In this study, S. equi isolates from different exporters had different SeM alleles. Attention to the hygiene status of farms will be necessary to prevent the incursion of strangles.
Publication Date: 2019-04-24 PubMed ID: 31019139PubMed Central: PMC6612491DOI: 10.1292/jvms.18-0656Google Scholar: Lookup
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- Journal Article
Summary
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The research article focuses on the genetic analysis of a bacteria named Streptococcus equi subsp. equi, which causes an infectious disease known as strangles in horses, specifically those imported into Japan.
Objective of the Research
- The primary objective of the study is to investigate the genotypes of Streptococcus equi subsp. equi (the bacteria causing strangles) from horses imported into Japan over an eight-year period.
Research Methodology
- The researchers have mined the SeM genotypes of S. equi isolated from those imported horses. The genotyping allowed them to classify 12 strains from multiple horses and exporters into four distinct allelic groups.
- This genotyping and comparison were performed to identify any genetic differences in the bacteria isolated from different exporters.
Findings of the Research
- The identified alleles or genetic variations in the bacteria were distinct from past isolates found in Japan, showing that the imported horses carried different strains of the bacteria.
- The research also revealed that the same allele was present in four strains isolated from horses from a single exporter over several years, indicating a specific pattern or consistency in the genetic configuration of the bacteria from this particular exporter.
Implications and Conclusions
- From the findings, it could be gathered that the S. equi isolates from different exporters had different SeM alleles. This variation in the bacterial strains across exporters indicates that the disease’s spread might be controlled better through appropriate preventive measures.
- The study concludes that increased attention to the hygiene status of horse farms could be necessary to prevent the occurrence and spread of strangles, especially considering the isolated bacteria’s genetic composition from imported horses.
Cite This Article
APA
Kasuya K, Tanaka N, Oshima F, Fujisawa N, Saito M, Tagami K, Niwa H, Sasai K.
(2019).
Genetic analysis of Streptococcus equi subsp. equi isolated from horses imported into Japan.
J Vet Med Sci, 81(6), 924-927.
https://doi.org/10.1292/jvms.18-0656 Publication
Researcher Affiliations
- Moji Branch Shinmoji Quarantine Facility, Animal Quarantine Service, MAFF, 3-1-2 Shinmojikita, Moji, Kitakyushu, Fukuoka 800-0113, Japan.
- Moji Branch Shinmoji Quarantine Facility, Animal Quarantine Service, MAFF, 3-1-2 Shinmojikita, Moji, Kitakyushu, Fukuoka 800-0113, Japan.
- Moji Branch Shinmoji Quarantine Facility, Animal Quarantine Service, MAFF, 3-1-2 Shinmojikita, Moji, Kitakyushu, Fukuoka 800-0113, Japan.
- Moji Branch Shinmoji Quarantine Facility, Animal Quarantine Service, MAFF, 3-1-2 Shinmojikita, Moji, Kitakyushu, Fukuoka 800-0113, Japan.
- Moji Branch Kagoshima Airport Sub-branch, Animal Quarantine Service, MAFF, 1590-5 Kareigawa, Hayatocho, Kirishima, Kagoshima, 899-5113, Japan.
- Moji Branch Shinmoji Quarantine Facility, Animal Quarantine Service, MAFF, 3-1-2 Shinmojikita, Moji, Kitakyushu, Fukuoka 800-0113, Japan.
- Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Laboratory of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-oraikita, Izumisano, Osaka 598-8531, Japan.
MeSH Terms
- Animals
- Bacterial Proteins / genetics
- Horse Diseases / epidemiology
- Horse Diseases / microbiology
- Horses
- Japan / epidemiology
- Lymphadenitis / microbiology
- Lymphadenitis / veterinary
- Streptococcal Infections / microbiology
- Streptococcal Infections / veterinary
- Streptococcus equi / genetics
- Streptococcus equi / isolation & purification
References
This article includes 18 references
- Anzai T, Kuwamoto Y, Wada R, Sugita S, Kakuda T, Takai S, Higuchi T, Timoney JF. Variation in the N-terminal region of an M-like protein of Streptococcus equi and evaluation of its potential as a tool in epidemiologic studies.. Am J Vet Res 2005 Dec;66(12):2167-71.
- Anzai T, Nakanishi A, Wada R, Higuchi T, Hagiwara S, Takazawa M, Oobayashi K, Inoue T. Isolation of Streptococcus equi subsp. equi from thoroughbred horses in a racehorse-breeding area of Japan.. J Vet Med Sci 1997 Nov;59(11):1031-3.
- Anzai T, Timoney JF, Kuwamoto Y, Fujita Y, Wada R, Inoue T. In vivo pathogenicity and resistance to phagocytosis of Streptococcus equi strains with different levels of capsule expression.. Vet Microbiol 1999 Jul 1;67(4):277-86.
- Harrington DJ, Sutcliffe IC, Chanter N. The molecular basis of Streptococcus equi infection and disease.. Microbes Infect 2002 Apr;4(4):501-10.
- Hobo S, Muranaka M, Niwa H, Uchiyama T, Yoda M, Suzuki Y, Maeda M. Application of serodiagnostic test specific to strangles in strangles outbreak and genetic analysis of Streptococcus equi isolates. Nippon Juishikai Zasshi 63: 696–701(in Japanese).
- Ivens PA, Matthews D, Webb K, Newton JR, Steward K, Waller AS, Robinson C, Slater JD. Molecular characterisation of 'strangles' outbreaks in the UK: the use of M-protein typing of Streptococcus equi ssp. equi.. Equine Vet J 2011 May;43(3):359-64.
- Katayama M, Miyama M, Furuya S, Kuwamoto Y, Hobo S, Anzai T. Epidemiological analysis of a herd infected with strangles by imported carrier horse. Nippon Juishikai Zasshi 56: 139–143(in Japanese).
- Kelly C, Bugg M, Robinson C, Mitchell Z, Davis-Poynter N, Newton JR, Jolley KA, Maiden MC, Waller AS. Sequence variation of the SeM gene of Streptococcus equi allows discrimination of the source of strangles outbreaks.. J Clin Microbiol 2006 Feb;44(2):480-6.
- Libardoni F, Vielmo A, Farias L, Matter LB, Pötter L, Spilki FR, de Vargas AC. Diversity of seM in Streptococcus equi subsp. equi isolated from strangles outbreaks.. Vet Microbiol 2013 Mar 23;162(2-4):663-669.
- Lindahl S, Söderlund R, Frosth S, Pringle J, Båverud V, Aspán A. Tracing outbreaks of Streptococcus equi infection (strangles) in horses using sequence variation in the seM gene and pulsed-field gel electrophoresis.. Vet Microbiol 2011 Nov 21;153(1-2):144-9.
- Parkinson NJ, Robin C, Newton JR, Slater J, Waller AS. Molecular epidemiology of strangles outbreaks in the UK during 2010.. Vet Rec 2011 Jun 25;168(25):666.
- Prescott JF, Srivastava SK, deGannes R, Barnum DA. A mild form of strangles caused by an atypical Streptococcus equi.. J Am Vet Med Assoc 1982 Feb 1;180(3):293-9.
- Spanier JG, Timoney JF. Bacteriophages of Streptococcus equi.. J Gen Virol 1977 May;35(2):369-75.
- Sweeney CR, Timoney JF, Newton JR, Hines MT. Streptococcus equi infections in horses: guidelines for treatment, control, and prevention of strangles.. J Vet Intern Med 2005 Jan-Feb;19(1):123-34.
- Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.. Nucleic Acids Res 1994 Nov 11;22(22):4673-80.
- Timoney JF. The pathogenic equine streptococci.. Vet Res 2004 Jul-Aug;35(4):397-409.
- Timoney JF, Timoney PJ, Strickland KL. Lysogeny and the immunologically reactive proteins of Streptococcus equi.. Vet Rec 1984 Aug 18;115(7):148-9.
- Yasuda M., Kamikaga T., Maekawa T., Saitoh H., Nakagawa Y., Sugiyama H., Watanabe T., Kobayashi M.1993. J. Hokkaido. Vet. Med. Assoc. 37: 110–112(in Japanese).
Citations
This article has been cited 1 times.- Rotinsulu DA, Ewers C, Kerner K, Amrozi A, Soejoedono RD, Semmler T, Bauerfeind R. Molecular Features and Antimicrobial Susceptibilities of Streptococcus equi ssp. equi Isolates from Strangles Cases in Indonesia.. Vet Sci 2023 Jan 10;10(1).
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