Physiological reports2020; 8(10); e14442; doi: 10.14814/phy2.14442

High-intensity training in normobaric hypoxia enhances exercise performance and aerobic capacity in Thoroughbred horses: A randomized crossover study.

Abstract: We examined the effects of high-intensity training in normobaric hypoxia on aerobic capacity and exercise performance in horses and the individual response to normoxic and hypoxic training. Eight untrained horses were studied in a randomized, crossover design after training in hypoxia (HYP; 15.0% inspired O ) or normoxia (NOR; 20.9% inspired O ) 3 days/week for 4 weeks separated by a 4-month washout period. Before and after each training period, incremental treadmill exercise tests were performed in normoxia. Each training session consisted of 1 min cantering at 7 m/s and 2 min galloping at the speed determined to elicit maximal oxygen consumption ( O max) in normoxia. Hypoxia increased significantly more than NOR in run time to exhaustion (HYP, +28.4%; NOR, +10.4%, p = .001), O max (HYP, +12.1%; NOR, +2.6%, p = .042), cardiac output ( ; HYP, +11.3%; NOR, -1.7%, p = .019), and stroke volume (SV) at exhaustion (HYP, +5.4%; NOR, -5.5%, p = .035) after training. No significant correlations were observed between NOR and HYP for individual changes after training in run time (p = .21), O max (p = .99), (p = .19), and SV (p = .46) at exhaustion. Arterial O saturation during exercise in HYP was positively correlated with the changes in run time (r = .85, p = .0073), (r = .72, p = .043) and SV (r = .77, p = .026) of HYP after training, whereas there were no correlations between these parameters in NOR. These results suggest that high-intensity training in normobaric hypoxia improved exercise performance and aerobic capacity of horses to a greater extent than the same training protocol in normoxia, and the severity of hypoxemia during hypoxic exercise might be too stressful for poor responders to hypoxic training.
Publication Date: 2020-05-23 PubMed ID: 32441408PubMed Central: PMC7243200DOI: 10.14814/phy2.14442Google Scholar: Lookup
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  • Journal Article
  • Randomized Controlled Trial
  • Veterinary
  • Research Support
  • Non-U.S. Gov't

Summary

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This study investigates whether high-intensity training under hypoxic conditions (reduced oxygen) can improve aerobic capacity and performance in horses. The findings indicate that such training gives superior results compared to normal oxygen conditions, though the reduced oxygen may pose challenges for animals not adapted to hypoxic training.

Research Design and Methods

The research conducted involved eight untrained horses participating in a controlled, randomized crossover experimental design. Several parameters were considered:

  • The hypoxic group: Where horses were trained under hypoxic conditions (15.0% Oxygen)
  • The normoxic group: Where horses were trained under normoxic conditions (normal atmospheric oxygen at 20.9%)
  • Both training procedures were carried out three days a week for a four-week period

Before and after each training session, incremental treadmill exercises were performed in a normoxic environment to test their impact. Each training session consisted of one minute of cantering at 7 m/s and two minutes of galloping at maximum oxygen consumption speed.

Key Findings

Here, various physiological parameters were observed to measure improvement in aerobic capacity and performance of the horses. The values are significantly higher under hypoxia than normoxia:

  • Run time to exhaustion: +28.4% in hypoxia vs. +10.4% in normoxia
  • Maximum oxygen consumption: +12.1% in hypoxia vs. +2.6% in normoxia
  • Cardiac output: +11.3% in hypoxia vs. -1.7% in normoxia
  • Stroke volume at exhaustion: +5.4% in hypoxia vs. -5.5% in normoxia

Implications of the Study

From the results, the study concludes that high-intensity training in hypoxia leads to a greater enhancement in the exercise performance and aerobic capacity of the horses when compared to normoxic training.

Another crucial observation was found concerning arterial oxygen saturation during exercise in hypoxia. There was a positive correlation between this parameter and changes in run time, cardiac output, and stroke volume after hypoxic training, indicating that how an individual horse responds to hypoxia could affect the benefits it receives from such training. However, there was no observed correlation between these parameters in normoxia.

Lastly, they point out that the severity of hypoxia-induced hypoxemia (limited oxygen supply in the body) during exercise could potentially be too stressful for horses that are poor responders to hypoxic training.

Cite This Article

APA
Mukai K, Ohmura H, Matsui A, Aida H, Takahashi T, Jones JH. (2020). High-intensity training in normobaric hypoxia enhances exercise performance and aerobic capacity in Thoroughbred horses: A randomized crossover study. Physiol Rep, 8(10), e14442. https://doi.org/10.14814/phy2.14442

Publication

ISSN: 2051-817X
NlmUniqueID: 101607800
Country: United States
Language: English
Volume: 8
Issue: 10
Pages: e14442
PII: e14442

Researcher Affiliations

Mukai, Kazutaka
  • Sports Science Division, Equine Research Institute, Japan Racing Association, Utsunomiya, Tochigi, Japan.
Ohmura, Hajime
  • Sports Science Division, Equine Research Institute, Japan Racing Association, Utsunomiya, Tochigi, Japan.
Matsui, Akira
  • Sports Science Division, Equine Research Institute, Japan Racing Association, Utsunomiya, Tochigi, Japan.
Aida, Hiroko
  • Sports Science Division, Equine Research Institute, Japan Racing Association, Utsunomiya, Tochigi, Japan.
Takahashi, Toshiyuki
  • Sports Science Division, Equine Research Institute, Japan Racing Association, Utsunomiya, Tochigi, Japan.
Jones, James H
  • Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, USA.

MeSH Terms

  • Adaptation, Physiological / physiology
  • Animals
  • Cross-Over Studies
  • Exercise Tolerance / physiology
  • Female
  • Horses
  • Hypoxia / physiopathology
  • Male
  • Oxygen Consumption / physiology
  • Physical Conditioning, Animal
  • Running / physiology

Conflict of Interest Statement

This study was funded by the Japan Racing Association. KM, HO, AM, HA, and TT are employees of the Japan Racing Association.

References

This article includes 34 references

Citations

This article has been cited 4 times.
  1. Gutknecht AP, Gonzalez-Figueres M, Brioche T, Maurelli O, Perrey S, Favier FB. Maximizing anaerobic performance with repeated-sprint training in hypoxia: In search of an optimal altitude based on pulse oxygen saturation monitoring.. Front Physiol 2022;13:1010086.
    doi: 10.3389/fphys.2022.1010086pubmed: 36311239google scholar: lookup
  2. Mukai K, Kitaoka Y, Takahashi Y, Takahashi T, Takahashi K, Ohmura H. Moderate-intensity training in hypoxia improves exercise performance and glycolytic capacity of skeletal muscle in horses.. Physiol Rep 2021 Dec;9(23):e15145.
    doi: 10.14814/phy2.15145pubmed: 34889527google scholar: lookup
  3. van Doorslaer de Ten Ryen S, Francaux M, Deldicque L. Regulation of satellite cells by exercise in hypoxic conditions: a narrative review.. Eur J Appl Physiol 2021 Jun;121(6):1531-1542.
    doi: 10.1007/s00421-021-04641-4pubmed: 33745023google scholar: lookup
  4. Mukai K, Ohmura H, Takahashi Y, Kitaoka Y, Takahashi T. Four weeks of high-intensity training in moderate, but not mild hypoxia improves performance and running economy more than normoxic training in horses.. Physiol Rep 2021 Feb;9(4):e14760.
    doi: 10.14814/phy2.14760pubmed: 33611843google scholar: lookup