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Home / Equine Research Bank / PLoS One / Histological comparison of the lamellar tissue of Iberian or...
PloS one2023; 18(6); e0286536; doi: 10.1371/journal.pone.0286536

Histological comparison of the lamellar tissue of Iberian origin breed horses created in semi-feral conditions or in an intensive system.

Abstract: Although the external conformation of wild horse hooves has been proposed as an ideal model for domesticated modern horses, histological signs of laminitis have been reported among them. With the hypothesis that the lamellar tissue of horses of Iberian origin raised in semi-feral is healthier than those raised in an intensive management system (stall confinement and high-calorie diet intake), the objective was to compare their lamellar tissues. Lamellar tissue samples were taken from the forelimb hoof of eight domesticated Mangalarga Marchador (MM) horses and from six semi-feral Marajoara (MJ) and Puruca (MP) horses. Primary epidermal lamella (PEL) and secondary epidermal lamella (SEL) were measured (length and width) in several regions, and their shapes were morphologically classified into different types. Breed groups were compared using analysis of variance, followed by Tukey or Dunn tests (P<0.05). Early signs of laminitis such as abnormal keratinization of the abaxial primary dermal lamella, tapered tips of the axial PELs, pointed tips of the SEL, nuclei condensation, and abnormal spatial orientation of the secondary epidermal basal cells (SELBC), were seen only in MM horses. MP horses had a greater interface of epidermis/dermis contact than MJ horses and more rounded nuclei in the round SELBC than MM horses. In agreement with the study hypothesis, semi-feral MJ and MP horses' lamellae were classified as healthier than MM horses, which showed early signs of endocrinopathic laminitis.
Copyright: © 2023 Malacarne et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Publication Date: 2023-06-01 PubMed ID: 37262053PubMed Central: PMC10234555DOI: 10.1371/journal.pone.0286536Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the health of lamellar tissue in the hooves of horses of Iberian origin raised in wild conditions compared to those in an intensive system. It finds that semi-feral horses have healthier lamellae than those raised intensively, with the latter showing signs of laminitis.

Horses and Management Systems

  • The researchers compared horses of Iberian origin bred in two different environments: semi-feral conditions and an intensive management system.
  • Horses in the intensive management system were kept in stalls and provided with high-calorie diets, factors that are significantly different from conditions experienced by the semi-feral horses.

Methodology

  • Eight domesticated Mangalarga Marchador (MM) horses and six semi-feral Marajoara (MJ) and Puruca (MP) horses were studied.
  • Lamellar tissue samples, which are parts of the horse hoof, were taken from each of the horse’s forelimbs.
  • The primary and secondary epidermal lamellas (PEL and SEL) were measured in multiple regions and then morphologically classified into various types.

Results

  • Results revealed early signs of laminitis – a disease that causes inflammation in the tissues inside the hoof – only in the MM horses raised under the intensive system. Signs include unusual keratinization of primary dermal lamella, abnormal shapes of PEL and SEL tips, nuclei condensation, and irregular spatial orientation of secondary epidermal basal cells.
  • The semi-feral MP horses had greater interface contact between the epidermis and dermis compared to the MJ horses. Their round secondary epidermal basal cells had more rounded nuclei compared to the MM horses.

Conclusions

  • In line with their hypothesis, the researchers concluded that semi-feral MJ and MP horses have healthier lamellae or hoof tissue compared to MM horses raised in intensive management systems.
  • MM horses exhibited early signs of endocrinopathic laminitis, suggesting that their hoof health might be compromised due to their breeding environment and diet.

Cite This Article

APA
Malacarne BD, Martins RR, Paz CFR, Alves JVA, Dias LA, Cavalcante MA, Santos AM, Silva AGM, Leise BS, Carvalho AM, Faleiros RR. (2023). Histological comparison of the lamellar tissue of Iberian origin breed horses created in semi-feral conditions or in an intensive system. PLoS One, 18(6), e0286536. https://doi.org/10.1371/journal.pone.0286536

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 18
Issue: 6
Pages: e0286536

Researcher Affiliations

Malacarne, Bruno Dondoni
  • Marion DuPont Equine Medical Center, Virginia Tech, Leesburg, Virginia, United States of America.
  • Equinova Research Group, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Martins, Rodrigo Ribeiro
  • Equinova Research Group, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Centro Universitário de Mineiros-UNIFIMES, Mineiros, Goiás, Brazil.
Paz, Cahuê Francisco Rosa
  • Equinova Research Group, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Alves, João Victor Almeida
  • Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Dias, Lucas Antunes
  • Equinova Research Group, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Cavalcante, Marina Alcantara
  • Equinova Research Group, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Santos, Alison Miranda
  • Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará, Brazil.
Silva, André Guimarães Maciel
  • Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará, Brazil.
Leise, Britta Sigrid
  • Veterinary Clinical Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America.
Carvalho, Armando Mattos
  • Equinova Research Group, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Faleiros, Rafael Resende
  • Equinova Research Group, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

MeSH Terms

  • Horses
  • Animals
  • Foot Diseases / veterinary
  • Foot Diseases / pathology
  • Horse Diseases / pathology
  • Epidermis / pathology
  • Epidermal Cells
  • Dermatitis / pathology
  • Inflammation / pathology

Conflict of Interest Statement

The authors have declared that no competing interests exist.

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