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Home / Equine Research Bank / J Anat / Histological features of the dorsal cortex of the third meta...
Journal of anatomy1992; 181 ( Pt 3)(Pt 3); 455-469;

Histological features of the dorsal cortex of the third metacarpal bone mid-diaphysis during postnatal growth in thoroughbred horses.

Abstract: The dorsal cortex of the equine third metacarpal mid-diaphyseal bone was characterised during growth by the histological and microradiographic examination of specimens from 30 horses ranging in age from 2 months to 8 y. Bone from horses aged less than 6 months was characterised by rapid periosteal apposition of circumferential trabeculae of woven bone that were next connected by radial trabeculae to the parent cortex. Deposition of lamellar bone on the inner trabecular surfaces resulted in rows of primary osteons. Replacement of primary bone occurred only after 4 months of age and preferentially in the woven interstitial bone separating rows of primary osteons formed in the postnatal periosteal cortex. Resorption cavities and incompletely filled secondary osteons characterised bone of 1 and 2-y-old horses. Bone from horses older than 3 y contained several generations of secondary osteons, fewer resorption spaces and incompletely filled osteons, and had a greater portion of circumferentially oriented collagen fibres than bone from younger horses. Bone from horses older than 5 y had large resorption cavities characterised by irregular boundaries. We propose that the process of periosteal bone tissue apposition observed in growing foals be called 'saltatory primary osteonal bone formation' and that this process results in faster cortical expansion and larger total surface area for bone deposition than circumferential lamellar, simple primary osteonal, and plexiform mechanisms of periosteal bone formation. We speculate that bone from 1 and 2-y-old horses would be more susceptible to fatigue microdamage resulting from compressive loads because of high porosity, few completed secondary osteons and low proportion of circumferentially oriented collagen fibres.
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Publication Date: 1992-12-01 PubMed ID: 1304584PubMed Central: PMC1259699
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the histological changes in the dorsal cortex of the third metacarpal bone in thoroughbred horses during growth stages from 2 months to 8 years. The results indicate variations in bone development suggesting different levels of susceptibility to micro-damage due to factors such as porosity and collagen fiber orientation.

Research Method

  • The researchers conducted a histological and microradiographic examination of specimens sourced from 30 horses aging between 2 months and 8 years.
  • The focus was on the dorsal cortex of the third metacarpal mid-diaphyseal bone.

Findings

  • For horses less than 6 months old, there was rapid periosteal apposition of circumferential trabeculae of woven bone immediately connected by radial trabeculae to the parent cortex. This resulted in rows of primary osteons due to the deposition of lamellar bone on inner trabecular surfaces.
  • Primary bone replacement started only after the horse was 4 months old, mainly in the woven interstitial bone separating rows of primary osteons formed in the postnatal periosteal cortex.
  • In 1 and 2-year-old horses, the bone exhibit resorption cavities and incompletely filled secondary osteons.
  • Bone from horses older than 3 years contained multiple generations of secondary osteons, a lower number of resorption spaces and incompletely filled osteons compared to younger horses. Additionally, the collagen fibres were more circumferentially oriented.
  • In horses older than 5 years, large resorption cavities with irregular boundaries were observed.

Propositions and Speculations

  • The researchers proposed a new term for the observed process of periosteal bone tissue apposition in growing foals: ‘saltatory primary osteonal bone formation.’ They suggest this process results in rapid cortical expansion and provides a larger total surface area for bone deposition compared to other mechanisms of periosteal bone formation.
  • The study speculates that bones in 1 and 2-year-old horses would be more susceptible to fatigue microdamage as a result of compressive loads. This is attributed to greater porosity, a lower number of completed secondary osteons, and a lower proportion of circumferentially oriented collagen fibres.

Cite This Article

APA
Stover SM, Pool RR, Martin RB, Morgan JP. (1992). Histological features of the dorsal cortex of the third metacarpal bone mid-diaphysis during postnatal growth in thoroughbred horses. J Anat, 181 ( Pt 3)(Pt 3), 455-469.

Publication

Journal of anatomy
ISSN: 0021-8782
NlmUniqueID: 0137162
Country: England
Language: English
Volume: 181 ( Pt 3)
Issue: Pt 3
Pages: 455-469

Researcher Affiliations

Stover, S M
  • Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, University of California, Davis 95616.
Pool, R R
    Martin, R B
      Morgan, J P

        MeSH Terms

        • Aging / physiology
        • Animals
        • Bone Remodeling
        • Bone Resorption / pathology
        • Collagen
        • Female
        • Horses / anatomy & histology
        • Male
        • Metacarpus / anatomy & histology
        • Metacarpus / growth & development
        • Microradiography

        References

        This article includes 18 references
        1. SMITH JW, WALMSLEY R. Factors affecting the elasticity of bone.. J Anat 1959 Oct;93(Pt 4):503-23.
          pubmed: 13832048
        2. ENLOW DH. A study of the post-natal growth and remodeling of bone.. Am J Anat 1962 Mar;110:79-101.
          pubmed: 13890322doi: 10.1002/aja.1001100202google scholar: lookup
        3. Turner AS, Mills EJ, Gabel AA. In vivo measurement of bone strain in the horse.. Am J Vet Res 1975 Nov;36(11):1573-9.
          pubmed: 1190599
        4. Arsenault AL. Vascular canals in bovine cortical bone studied by corrosion casting.. Calcif Tissue Int 1990 Nov;47(5):320-5.
          pubmed: 2257526doi: 10.1007/BF02555916google scholar: lookup
        5. Nunamaker DM, Butterweck DM, Provost MT. Fatigue fractures in thoroughbred racehorses: relationships with age, peak bone strain, and training.. J Orthop Res 1990 Jul;8(4):604-11.
          pubmed: 2355300doi: 10.1002/jor.1100080417google scholar: lookup
        6. Evans FG, Vincentelli R. Relations of the compressive properties of human cortical bone to histological structure and calcification.. J Biomech 1974 Jan;7(1):1-10.
          pubmed: 4820647doi: 10.1016/0021-9290(74)90064-5google scholar: lookup
        7. Lipson SF, Katz JL. The relationship between elastic properties and microstructure of bovine cortical bone.. J Biomech 1984;17(4):231-40.
          pubmed: 6736060doi: 10.1016/0021-9290(84)90134-9google scholar: lookup
        8. Martin RB, Burr DB. A hypothetical mechanism for the stimulation of osteonal remodelling by fatigue damage.. J Biomech 1982;15(3):137-9.
          pubmed: 7096366doi: 10.1016/s0021-9290(82)80001-8google scholar: lookup
        9. EVANS FG. Relations between the microscopic structure and tensile strength of human bone.. Acta Anat (Basel) 1958;35(4):285-301.
          pubmed: 13626400doi: 10.1159/000141417google scholar: lookup
        10. FROST HM. In vivo osteocyte death.. J Bone Joint Surg Am 1960 Jan;42-A:138-43.
          pubmed: 13849861
        11. ENLOW DH. Functions of the Haversian system.. Am J Anat 1962 May;110:269-305.
          pubmed: 13890323doi: 10.1002/aja.1001100305google scholar: lookup
        12. Smith JW. Collagen fibre patterns in mammalian bone.. J Anat 1960 Jul;94(Pt 3):329-44.
          pubmed: 17105115
        13. Carter DR, Hayes WC. Compact bone fatigue damage: a microscopic examination.. Clin Orthop Relat Res 1977;(127):265-74.
          pubmed: 912990
        14. Carter DR, Hayes WC. Fatigue life of compact bone--I. Effects of stress amplitude, temperature and density.. J Biomech 1976;9(1):27-34.
          pubmed: 1249078doi: 10.1016/0021-9290(76)90136-6google scholar: lookup
        15. Burr DB, Martin RB, Schaffler MB, Radin EL. Bone remodeling in response to in vivo fatigue microdamage.. J Biomech 1985;18(3):189-200.
          pubmed: 3997903doi: 10.1016/0021-9290(85)90204-0google scholar: lookup
        16. Portigliatti Barbos M, Bianco P, Ascenzi A, Boyde A. Collagen orientation in compact bone: II. Distribution of lamellae in the whole of the human femoral shaft with reference to its mechanical properties.. Metab Bone Dis Relat Res 1984;5(6):309-15.
          pubmed: 6493042doi: 10.1016/0221-8747(84)90018-3google scholar: lookup
        17. CURREY JD. Differences in the tensile strength of bone of different histological types.. J Anat 1959 Jan;93(1):87-95.
          pubmed: 13620620
        18. Carter DR, Spengler DM. Mechanical properties and composition of cortical bone.. Clin Orthop Relat Res 1978 Sep;(135):192-217.
          pubmed: 361320

        Citations

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        1. Doube M. Closing cones create conical lamellae in secondary osteonal bone. R Soc Open Sci 2022 Aug;9(8):220712.
          doi: 10.1098/rsos.220712pubmed: 35958092google scholar: lookup
        2. Tomassini RL, Pesquero MD, Garrone MC, Marin-Monfort MD, Cerda IA, Prado JL, Montalvo CI, Fernández-Jalvo Y, Alberdi MT. First osteohistological and histotaphonomic approach of Equus occidentalis Leidy, 1865 (Mammalia, Equidae) from the late Pleistocene of Rancho La Brea (California, USA). PLoS One 2021;16(12):e0261915.
          doi: 10.1371/journal.pone.0261915pubmed: 34962948google scholar: lookup
        3. Taguchi T, Lopez MJ. An overview of de novo bone generation in animal models. J Orthop Res 2021 Jan;39(1):7-21.
          doi: 10.1002/jor.24852pubmed: 32910496google scholar: lookup
        4. Dzierzęcka M, Jaworski M, Purzyc H, Barszcz K. Regional Differences of Densitometric and Geometric Parameters of the Third Metacarpal Bone in Coldblood Horses - pQCT Study. J Vet Res 2017 Mar;61(1):111-120.
          doi: 10.1515/jvetres-2017-0014pubmed: 29978062google scholar: lookup
        5. Nacarino-Meneses C, Köhler M. Limb bone histology records birth in mammals. PLoS One 2018;13(6):e0198511.
          doi: 10.1371/journal.pone.0198511pubmed: 29924818google scholar: lookup
        6. Nacarino-Meneses C, Jordana X, Köhler M. Histological variability in the limb bones of the Asiatic wild ass and its significance for life history inferences. PeerJ 2016;4:e2580.
          doi: 10.7717/peerj.2580pubmed: 27761353google scholar: lookup
        7. Barrera JW, Le Cabec A, Barak MM. The orthotropic elastic properties of fibrolamellar bone tissue in juvenile white-tailed deer femora. J Anat 2016 Oct;229(4):568-76.
          doi: 10.1111/joa.12500pubmed: 27231028google scholar: lookup
        8. Kohara Y, Soeta S, Izu Y, Amasaki H. Accumulation of type VI collagen in the primary osteon of the rat femur during postnatal development. J Anat 2015 May;226(5):478-88.
          doi: 10.1111/joa.12296pubmed: 25943007google scholar: lookup
        9. Martinez-Maza C, Alberdi MT, Nieto-Diaz M, Prado JL. Life-history traits of the Miocene Hipparion concudense (Spain) inferred from bone histological structure. PLoS One 2014;9(8):e103708.
          doi: 10.1371/journal.pone.0103708pubmed: 25098950google scholar: lookup
        10. Kierdorf U, Flohr S, Gomez S, Landete-Castillejos T, Kierdorf H. The structure of pedicle and hard antler bone in the European roe deer (Capreolus capreolus): a light microscope and backscattered electron imaging study. J Anat 2013 Oct;223(4):364-84.
          doi: 10.1111/joa.12091pubmed: 23961846google scholar: lookup
        11. Wojda SJ, McGee-Lawrence ME, Gridley RA, Auger J, Black HL, Donahue SW. Yellow-bellied marmots (Marmota flaviventris) preserve bone strength and microstructure during hibernation. Bone 2012 Jan;50(1):182-8.
          doi: 10.1016/j.bone.2011.10.013pubmed: 22037004google scholar: lookup
        12. McGee ME, Miller DL, Auger J, Black HL, Donahue SW. Black bear femoral geometry and cortical porosity are not adversely affected by ageing despite annual periods of disuse (hibernation). J Anat 2007 Feb;210(2):160-9.
          doi: 10.1111/j.1469-7580.2006.00681.xpubmed: 17261138google scholar: lookup
        13. Firth EC. The response of bone, articular cartilage and tendon to exercise in the horse. J Anat 2006 Apr;208(4):513-26.
          doi: 10.1111/j.1469-7580.2006.00547.xpubmed: 16637875google scholar: lookup
        14. Tanck E, Hannink G, Ruimerman R, Buma P, Burger EH, Huiskes R. Cortical bone development under the growth plate is regulated by mechanical load transfer. J Anat 2006 Jan;208(1):73-9.
          doi: 10.1111/j.1469-7580.2006.00503.xpubmed: 16420380google scholar: lookup
        15. Skedros JG, Hunt KJ. Does the degree of laminarity correlate with site-specific differences in collagen fibre orientation in primary bone? An evaluation in the turkey ulna diaphysis. J Anat 2004 Aug;205(2):121-34.
          doi: 10.1111/j.0021-8782.2004.00318.xpubmed: 15291795google scholar: lookup
        16. de Margerie E. Laminar bone as an adaptation to torsional loads in flapping flight. J Anat 2002 Dec;201(6):521-6.
          doi: 10.1046/j.1469-7580.2002.00118.xpubmed: 12489764google scholar: lookup
        17. Gabrie A, Detilleux J, Jolly S, Reginster J-Y, Collin B, Dessy-Doizé C. Morphometric study of the equine navicular bone: age-related changes and influence of exercise. Vet Res Commun 1999 Jan;23(1):15-40.
          doi: 10.1023/a:1006102921304pubmed: 10905816google scholar: lookup
        18. Irandoust S, Whitton C, Henak C, Muir P. Tuning and validation of a virtual mechanical testing pipeline for condylar stress fracture risk assessment in Thoroughbred racehorses. R Soc Open Sci 2025 May;12(5):241935.
          doi: 10.1098/rsos.241935pubmed: 40370600google scholar: lookup
        19. Izu Y, Ishikawa H, Soeta S. Developmental process and homeostasis of whale long bones lacking medullary cavity using the radius of Antarctic minke whales, Balaenoptera bonaerensis. J Vet Med Sci 2025 Apr 1;87(4):336-348.
          doi: 10.1292/jvms.24-0430pubmed: 39938892google scholar: lookup
        20. Costa da Silva RG, Sun TC, Mishra AP, Boyde A, Doube M, Riggs CM. Intracortical remodelling increases in highly loaded bone after exercise cessation. J Anat 2024 Mar;244(3):424-437.
          doi: 10.1111/joa.13969pubmed: 37953410google scholar: lookup
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