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Home / Equine Research Bank / BMC Cell Biol / Horse bone marrow mesenchymal stem cells express embryo stem...
BMC cell biology2009; 10; 29; doi: 10.1186/1471-2121-10-29

Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12.

Abstract: Mesenchymal stem cells (MSCs) have been recently investigated for their potential use in regenerative medicine. MSCs, in particular, have great potential, as in various reports they have shown pluripotency for differentiating into many different cell types. However, the ability of MSCs to differentiate into tendon cells in vitro has not been fully investigated. Results: In this study, we show that equine bone marrow mesenchymal stem cells (BM-MSCs), defined by their expression of markers such as Oct4, Sox-2 and Nanog, have the capability to differentiate in tenocytes. These differentiated cells express tendon-related markers including tenomodulin and decorin. Moreover we show that the same BM-MSCs can differentiate in osteocytes, as confirmed by alkaline phosphatase and von Kossa staining. Conclusions: As MSCs represent an attractive tool for tendon tissue repair strategies, our data suggest that bone marrow should be considered the preferred MSC source for therapeutic approaches.
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Publication Date: 2009-04-22 PubMed ID: 19383177PubMed Central: PMC2678092DOI: 10.1186/1471-2121-10-29Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the potential of a certain type of horse stem cells in regenerating tissues, particularly tendons.

Introduction and Aim

  • The research delves into the potential of Mesenchymal stem cells (MSCs) – a specific kind of stem cells in regenerative medicine. Stem cells are cells with the potential to develop into many different types of cells in the body.
  • The researchers specifically aim to investigate the ability of these MSCs to differentiate into tendon cells when cultured in a lab.

Results and Findings

  • The study shows that certain markers specifically Oct4, Sox-2, and Nanog, are expressed by the equine bone marrow mesenchymal stem cells (BM-MSCs), these indicate their stemness or the ability to change into different types of cells.
  • The researchers claim that these BM-MSCs can differentiate into tenocytes or tendon cells. These differentiated cells were observed to express tenomodulin and decorin which are specific to tendon-related cells.
  • The study also reveals that the same BM-MSCs have the ability to differentiate into osteocytes or bone cells. Alkaline phosphatase and von Kossa staining, common techniques to validate bone cells, were used to confirm this differentiation.

Conclusions

  • The researchers conclude that MSCs have a promising significance in tendon tissue repair strategies. They suggest that the bone marrow, based on their findings, should be considered as a primary source of MSCs for such therapeutic approaches.

Cite This Article

APA
Violini S, Ramelli P, Pisani LF, Gorni C, Mariani P. (2009). Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12. BMC Cell Biol, 10, 29. https://doi.org/10.1186/1471-2121-10-29

Publication

BMC cell biology
ISSN: 1471-2121
NlmUniqueID: 100966972
Country: England
Language: English
Volume: 10
Pages: 29

Researcher Affiliations

Violini, Stefania
  • Livestock Genomics Unit, Parco Tecnologico Padano, CERSA, Via Einstein, Loc Cascina Codazza, , Lodi 26900, Italy. stefania.violini@tecnoparco.org
Ramelli, Paola
    Pisani, Laura F
      Gorni, Chiara
        Mariani, Paola

          MeSH Terms

          • Animals
          • Biomarkers / metabolism
          • Bone Marrow Cells / cytology
          • Bone Morphogenetic Proteins / metabolism
          • Bone Morphogenetic Proteins / pharmacology
          • Cell Differentiation
          • Decorin
          • Embryonic Stem Cells / metabolism
          • Extracellular Matrix Proteins / metabolism
          • Gene Expression Regulation
          • Homeodomain Proteins / metabolism
          • Horses
          • Membrane Proteins / metabolism
          • Mesenchymal Stem Cells / cytology
          • Octamer Transcription Factor-3 / metabolism
          • Proteoglycans / metabolism
          • SOXB1 Transcription Factors / metabolism
          • Tendons / cytology

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