Analyze Diet
Animals : an open access journal from MDPI2026; 16(3); 482; doi: 10.3390/ani16030482

Identifying by Radiograph Grade 4 Aplasia of the Caudal Lamina Ventralis in the Equine Sixth Cervical Vertebra and Three Coinciding Morphological Variations.

Abstract: To date, radiographically identifying the bony landmarks relevant to diagnosing the complete C6 aplasia of the caudal lamina ventralis (C6 aCLV4) has not been described. Furthermore, a gross study has identified C6 aCLV4 as the main correlation between transposition of the CLV from C6 to C7, where coinciding neck pain was commonly reported. This study aimed to identify C6 aCLV4 in radiographs, where the outcome might benefit equine practitioners in isolating neck pain. Initially, the relevant bony landmarks were radiographically determined from a normal C6 by applying a lateral 30° dorsal-ventral oblique view. From here, the caudal border of the transverse process (TP) projecting from the vertebral body became the defining point of reference due to the image clearly demarcating the adjacent CLV. In C6 aCLV4 cases, the CLV is completely aplastic caudal to the TP. Twenty mixed-breed horses (13 males/7 females) aged 3-22 years radiographically demonstrated a C6 aCLV4, which was confirmed by their corresponding gross morphology. During this process three anomalous variations became apparent-in the longus colli muscle, C7 foramen transversarium, and vertebral artery. Therefore, this study demonstrates effective techniques for identifying C6 aCLV4 in horses, where the results might assist veterinarians in diagnosing neck pain while providing anatomical clarity.
Publication Date: 2026-02-04 PubMed ID: 41681463PubMed Central: PMC12897093DOI: 10.3390/ani16030482Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
  • Journal Article

Cite This Article

APA
DeClue A, Workman K, May-Davis S. (2026). Identifying by Radiograph Grade 4 Aplasia of the Caudal Lamina Ventralis in the Equine Sixth Cervical Vertebra and Three Coinciding Morphological Variations. Animals (Basel), 16(3), 482. https://doi.org/10.3390/ani16030482

Publication

ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 16
Issue: 3
PII: 482

Researcher Affiliations

DeClue, Audrey
  • DeClue Equine LLC, 6955 NW 100th Street, Ocala, FL 34482, USA.
Workman, Kate
  • Rexos Incorporated (5O1c3b-Nonprofit Research Facility), 1087 Lakebay Rd., Vass, Carthage, NC 28394, USA.
  • Denali Equine PLLC, 1087 Lakebay Rd., Vass, NC 208394, USA.
May-Davis, Sharon
  • Faculty of Science, Agriculture, Business and Law, University of New England, Armidale, NSW 2350, Australia.

Conflict of Interest Statement

Author Audrey DeClue is employed by the company “DeClue Equine LLC”. Author Kate Workman is employed by the company “Denali Equine PLLC”. The authors declare no conflicts of interest.

References

This article includes 55 references
  1. Steel J.H. Outlines of Equine Anatomy. Longmans and Co.; London, UK: 1876. p. 46.
  2. Arnold P., Amson E., Fischer M.S. Differential Scaling Patterns of Vertebrae and the Evolution of the Neck Length in Mammals. Evolution. 2017;71:1587–1599. doi: 10.1111/evo.13232.
    doi: 10.1111/evo.13232pubmed: 28323340google scholar: lookup
  3. May-Davis S., Hunter R., White R. Morphology of the Ventral Process of the Sixth Cervical Vertebra in Extinct and Extant Equus: Functional Implications. Animals. 2023;13:1672. doi: 10.3390/ani13101672.
    doi: 10.3390/ani13101672pmc: PMC10215204pubmed: 37238101google scholar: lookup
  4. Sisson S. A Textbook of Veterinary Anatomy. W.B. Saunders; Philadelphia, PA, USA: 1910. p. 31.
  5. Sisson S., Grossman J.D. The Anatomy of the Domestic Animals. 4th ed. W.B. Saunders; Philadelphia, PA, USA: 1953. pp. 28–30. Preface.
  6. Stubbs G. Anatomy of the Horse. J. Purser; London, UK: 1766.
  7. Liautard A.F.A. Vade Mecum of Equine Anatomy. American Veterinary College; New York, NY, USA: 1879. p. 120.
  8. Ballou W.R. A Compend of Equine Anatomy and Physiology (1890) P. Blakiston’s Son and Co; Philadelphia, PA, USA: 1907. p. 12.
  9. Schmaltz R. Atlas der Anatomie des Pferdes. Verlagsbuchhandlung von Richard Schoetz; Berlin, Germany: 1924. Tafel 17a.
  10. Cave A.J.E. The morphology of the mammalian cervical pleuropophysis. J. Zool. 1975;177:377–393. doi: 10.1111/j.1469-7998.1975.tb02240.x.
  11. Arnold P. Evolution of the Mammalian Neck and Development, Morpho-functional, and Paleontological Perspectives. J. Mamm. Evol. 2021;28:173–183. doi: 10.1007/s10914-020-09506-9.
  12. Popesko P. Atlas of Topographical Anatomy of the Domestic Animals. 4th ed. Volume 4. W.B. Saunders; Philadelphia, PA, USA: 1985. p. 185.
  13. Butler L.A., Colles C.M., Dyson S.J., Kold S.E., Poulos P.W. Clinical Radiology of the Horse. 4th ed. Wiley Blackwell Pub; Hoboken, NJ, USA: 2017.
  14. International Committee on Veterinary Gross Anatomical Nomenclature . In: Nomina Anatomica Veterinaria. 6th ed. Gasse H., Van den Broeck W., Simeons P., editors. World Association of Veterinary Anatomists; Hanover, Germany: 2017. p. 17.
  15. Getty R. Equine Osteology. In: Sisson S., Grossman J.D., editors. The Anatomy of the Domestic Animals. 5th ed. W.B. Saunders; Philadelphia, PA, USA: 1975. pp. 258–259.
  16. DeRouen A., Spriet M., Aleman M. Prevalence of Anatomical Variation of the Sixth Cervical Vertebra and Association with Vertebral Canal Stenosis and Articular Process Osteoarthritis in the Horse. Vet. Radiol. Ultrasound. 2016;57:253–258. doi: 10.1111/vru.12350.
    doi: 10.1111/vru.12350pubmed: 26915973google scholar: lookup
  17. Beccati F., Pepe M., Santinelli I., Gialletti R., Di Meo A., Romero J.M. Radiographic Findings hand Anatomical Variations of the Caudal Cervical Area in Horses with Neck Pain and Ataxia: Case–Control Study on 116 Horses. Vet. Rec. 2020;187:e79. doi: 10.1136/vr.105756.
    doi: 10.1136/vr.105756pubmed: 33033105google scholar: lookup
  18. Lindgren C.M., Wright L., Kristofferson M., Puchalski S.M. Computed tomography and myelography of the equine cervical spine: 180 cases (2013–2018) Equine Vet. Educ. 2020;33:475–483. doi: 10.1111/eve.13350.
    doi: 10.1111/eve.13350google scholar: lookup
  19. Ros K., Doveren A., Dreessen C., Pellman R., Beccati F., Zimmerman E., Distl O. Radiological Methods for the Imaging of Congenital Malformations of C6-T1, the First and Second Sternal Ribs and Development of a Classification System, Demonstrated in Warmblood Horses. Animals. 2023;13:3732. doi: 10.3390/ani13233732.
    doi: 10.3390/ani13233732pmc: PMC10705149pubmed: 38067084google scholar: lookup
  20. Henderson C.S., Story M.R., Nout-Lomas Y.S. Neck pain but not neurologic disease occurs more frequently in horses with transposition of the lamina ventralis from C6 to C7. J. Am. Vet. Med Assoc. 2024;29:1215–1221. doi: 10.2460/javma.24.04.0230.
    doi: 10.2460/javma.24.04.0230pubmed: 38810659google scholar: lookup
  21. Dyson S., Phillips K., Zheng S., Aleman M. Congenital variants of the ventral laminae of the sixth and seventh cervical vertebrae are not associated with clinical signs or other radiological abnormalities of the cervicothoracic region in Warmblood horses. Equine Vet. J. 2024;57:419–430. doi: 10.1111/evj.14127.
    doi: 10.1111/evj.14127pmc: PMC11807929pubmed: 38938125google scholar: lookup
  22. Veraa S., de Graaf K., Wijnberg I.D., Back W., Vernooij H., Nielen M., Belt A.J. Caudal Cervical Vertebral Morphological Variation is not Associated with Clinical Signs in Warmblood Horses. Equine Vet. J. 2019;52:210–224. doi: 10.1111/evj.13140.
    doi: 10.1111/evj.13140pmc: PMC7027909pubmed: 31211852google scholar: lookup
  23. Lofgren E.A., Voigt M.A., Brady C.M. Information-Seeking Behavior of the Horse Competition Industry: A Demographic Study. J. Equine Vet. Sci. 2016;37:58–62. doi: 10.1016/j.jevs.2015.10.005.
  24. Pickering P., Hockenhull J. Optimising the Efficacy of Equine Welfare Communications: Do Equine Stakeholders Differ in Their Information-Seeking Behavior and Communication Preferences? Animals. 2020;10:21. doi: 10.3390/ani10010021.
    doi: 10.3390/ani10010021pmc: PMC7022754pubmed: 31861909google scholar: lookup
  25. Fenner K., Hyde M., Crean A., McGreevy P. Identifying Sources of Potential Bias When Using Online Survey Data to Explore Horse Training, Management, and Behavior: A Systematic Review. Vet. Sci. 2020;7:140. doi: 10.3390/vetsci7030140.
    doi: 10.3390/vetsci7030140pmc: PMC7558402pubmed: 32971754google scholar: lookup
  26. Herbst A.C., Hartmann K.S., Brown D.O., Malinowski K. 2022 Equine community needs assessment survey. J. Equine Vet. Sci. 2024;142:105193. doi: 10.1016/j.jevs.2024.105193.
    doi: 10.1016/j.jevs.2024.105193pubmed: 39276953google scholar: lookup
  27. Varmazyar R., NikAhd H., Pinto A.L., González-Valiente C.L. Accuracy and quality of animal health information on social media: A case study on TikTok. Bibl. An. Investig. 2023;19:101–115.
  28. Moran R.E., Knesl O. How can the veterinary profession tackle social media misinformation? J. Am. Vet. Med. Assoc. 2025;263:802–808. doi: 10.2460/javma.24.10.0665.
    doi: 10.2460/javma.24.10.0665pubmed: 40009998google scholar: lookup
  29. Chauveau A. In: The Comparative Anatomy of the Domesticated Animals. 2nd ed. Fleming G., editor. D. Appleton and Company; New York, NY, USA: 1908. pp. 53–80.
  30. Gorton B. Abnormal Cervical Vertebra of Horse. J. Anat. 1923;57:380–381.
    pmc: PMC1263014pubmed: 17103989
  31. Gee C., Small A., Shorter K., Brown W.Y. A Radiographic Technique for Assessment of Morphologic Variations of the Equine Caudal Cervical Spine. Animals. 2020;10:667. doi: 10.3390/ani10040667.
    doi: 10.3390/ani10040667pmc: PMC7222808pubmed: 32290538google scholar: lookup
  32. Santinelli I., Beccati F., Arcelli R. Anatomical Variation of the Spinous and Transverse Processes in the Caudal Cervical Vertebrae and the First Thoracic Vertebra in Horses. Equine Vet. J. 2016;48:45–49. doi: 10.1111/evj.12397.
    doi: 10.1111/evj.12397pubmed: 25471336google scholar: lookup
  33. Crijns C.P., Broeckx B.J.G. Evaluation of cervical radiographs in Dutch Warmblood horses, using novel radiographic grading system for the cervical articular process joints. Equine Vet. Educ. 2021;33:593–601. doi: 10.1111/eve.13375.
    doi: 10.1111/eve.13375google scholar: lookup
  34. McNerney T., Cital S.N., Palmer D. From clicks to clinics: How credentialed veterinary technicians are changing veterinary medicine on social media. J. Am. Vet. Med. Assoc. 2025;263:S61–S63. doi: 10.2460/javma.25.03.0215.
    doi: 10.2460/javma.25.03.0215pubmed: 40639412google scholar: lookup
  35. May-Davis S. Congenital Malformations of the 1st Sternal Rib. J. Equine Vet. Sci. 2017;49:92–100. doi: 10.1016/j.jevs.2016.09.013.
  36. May-Davis S., Dzingle D., Saber E., Blades Eckelbarger P. Characterization of the Caudal Ventral Tubercle in the Sixth Cervical Vertebra in Modern Equus ferus caballus. Animals. 2023;13:2384. doi: 10.3390/ani13142384.
    doi: 10.3390/ani13142384pmc: PMC10376820pubmed: 37508161google scholar: lookup
  37. May-Davis S., Blades Eckelbarger P., Dzingle D., Saber E. Characterization and Association of the Missing Ventral Tubercle(s) from the Sixth Cervical Vertebra and Transpositions on the Ventral Surface of the Seventh Cervical Vertebra in Modern Equus ferus caballus. Animals. 2024;14:1830. doi: 10.3390/ani14121830.
    doi: 10.3390/ani14121830pmc: PMC11200614pubmed: 38929448google scholar: lookup
  38. Bradley O. The Topical Anatomy of the Head and Neck of the Horse. 2nd ed. Green and Sons; Edinburgh, UK: 1947. p. 26.
  39. Vasconcellos L.A.S. Morphological irregularity of tracheal rings in horses associated or not with cervical malformation: Initial study. Pubvet. 2024;18:e1588. doi: 10.31533/pubvet.v18n05e1588.
  40. May-Davis S., Walker C. Variations and Implications of the Gross Morphology in the Longus colli Muscle in Thoroughbred and Thoroughbred Derivative Horses Presenting with a Congenital Malformation of the Sixth and Seventh Cervical Vertebrae. J. Equine Vet. Sci. 2015;35:560–568. doi: 10.1016/j.jevs.2015.03.002.
  41. Sisson S. Equine Myology. In: Sisson S., Grossman J.D., editors. The Anatomy of the Domestic Animals. 5th ed. W.B. Saunders; Philadelphia, PA, USA: 1975. pp. 392–393.
  42. Rombach N., Stubbs N., Clayton H. Gross anatomy of the deep perivertebral musculature in horses. Am. J. Vet. Res. 2014;75:433–440. doi: 10.2460/ajvr.75.5.433.
    doi: 10.2460/ajvr.75.5.433pubmed: 24762014google scholar: lookup
  43. Gerlach K., Schad P., Offhaus J., Brehm W., Pelli A. Vorkommen von unterschiedlichen Ausprägungen der Processus Transversi im Bereich der letzten beiden Halswirbel des Pferdes. Pferdeheilkunde. 2021;37:605–610. doi: 10.21836/PEM20210606.
    doi: 10.21836/PEM20210606google scholar: lookup
  44. Tucker R., Anderson J., Schmidt S.M., Stavisky J. Disorders of the cervical vertebral column part 2: Update on current surgical techniques. Equine Vet. Educ. 2025;38:46–57. doi: 10.1111/eve.14192.
    doi: 10.1111/eve.14192google scholar: lookup
  45. Kowalczyk K.A., Majewski A. Analysis of surgical errors associated with anatomical variations clinically relevant in general surgery. Review of the literature. Transl. Res. Anat. 2021;23:100107. doi: 10.1016/j.tria.2020.100107.
  46. May-Davis S. The Occurrence of a Congenital Malformation in the Sixth and Seventh Cervical Vertebrae Predominantly Observed in Thoroughbred Horses. J. Equine Vet. Sci. 2014;18:1313–1317. doi: 10.1016/j.jevs.2014.09.012.
  47. Zimmerman E., Ros K.B., Pfarrer C., Distl O. Historic Horse Family Displaying Malformations of the Cervicothoracic Junction and Their Connection to Modern German Warmblood Horses. Animals. 2023;13:3415. doi: 10.3390/ani13213415.
    doi: 10.3390/ani13213415pmc: PMC10650596pubmed: 37958170google scholar: lookup
  48. Hendricks B.L. International Encyclopaedia of Horse Breeds. University of Oklahoma; Norman, OK, USA: 1995.
  49. Rousseau E. Horses of the World. Princeton University Press; Princeton, NJ, USA: 2014.
  50. Internet Search: Pedigree Online—All Breeds Database. [(accessed on 10 November 2025)]. Available online: https://www.allbreedspedigree.com/
  51. Denoix J.-M., Pailloux J.-P. Physical Therapy and Massage for the Horse. 2nd ed. CRC Press; London, UK: 2011.
  52. Astrup J., Gyntelberg F. Tension-type headache and low back pain reconsidered. Front. Neurol. 2022;13:912348. doi: 10.3389/fneur.2022.912348.
    doi: 10.3389/fneur.2022.912348pmc: PMC9372361pubmed: 35968274google scholar: lookup
  53. Kajimoto B.H.J., Addeo R.L.D., Campos G.C., Narazaki D.K., Correia L.S., Araújo M.P. Anatomical study of the vertebral artery path in human lower cervical spine. Acta Ortop. Bras. 2007;15:84–86. doi: 10.1590/S1413-78522007000200005.
  54. Tudose R.C., Rusu M.C., Hostiuc S. The Vertebral Artery: A Systematic Review and a Meta-Analysis of the Current Literature. Diagnostics. 2023;13:2036. doi: 10.3390/diagnostics13122036.
  55. Korim F., Kuricová M., Lipták T., Vilhanová Z., Eberlová L. Anomaly of the sixth cervical vertebra accompanied by atypical course of the vertebral artery in a dog. Vet. Rec. Case Rep. 2022;10:e305. doi: 10.1002/vrc2.305.
    doi: 10.1002/vrc2.305google scholar: lookup

Citations

This article has been cited 0 times.