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Veterinary immunology and immunopathology2011; 144(1-2); 147-154; doi: 10.1016/j.vetimm.2011.06.033

Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue.

Abstract: Bone marrow and adipose tissue are the two main sources of mesenchymal stem cell (MSC). The aim of this work was to analyse the immunophenotype of 7 surface markers and the expression of a panel of 13 genes coding for cell surface markers in equine bone marrow and adipose tissue-derived MSCs obtained from 9 horses at third passage. The tri-lineage differentiation was confirmed by specific staining. Equine MSCs from both sources were positive for the MSC markers CD29 and CD90, while were negative for CD44, CD73, CD105, CD45 and CD34. The gene expression of these molecules was also evaluated by reverse transcriptase real-time quantitative PCR along with the expression of 5 other MSC markers. Both populations of cells expressed CD13, CD29, CD44, CD49d, CD73, CD90, CD105, CD106, CD146 and CD166 transcripts. Significant differences in gene expression levels between BM- and AT-MSCs were observed for CD44, CD90, CD29 and CD34. Both cell types were negative for CD45 and CD31. The surface antigens tested revealed a similar phenotypic profile between horse and human MSCs, although specific differences in some surface antigens were noticed.
Copyright © 2011 Elsevier B.V. All rights reserved.
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Publication Date: 2011-07-02 PubMed ID: 21782255DOI: 10.1016/j.vetimm.2011.06.033Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study analyzes the characteristics of mesenchymal stem cells (MSCs) derived from horse bone marrow and adipose tissues. The research includes an examination of surface markers on the cells and the gene expression profiles associated with these markers.

Analysis of MSC Surface Markers

  • The researchers carried out a detailed examination of seven surface markers on MSCs. These surface markers serve as a sort of biological identification for cells and help scientists distinguish MSCs from other types of cells.
  • They employed MSCs derived from the bone marrow and adipose tissues of nine horses for this experiment, with cells at their third growth cycle or passage used for the analysis.
  • The surface markers the researchers examined were CD29, CD90, CD44, CD73, CD105, CD45, and CD34. The MSCs from both sources tested positive for CD29 and CD90, indicating they were indeed MSCs. However, they were negative for the rest of the markers. This could possibly indicate a difference in species, as some markers that are typically present on human MSCs were absent in horse MSCs.

Gene Expression in MSCs

  • Apart from the surface marker analysis, the researchers also investigated gene expression for 13 genes coding for these surface markers. In this context, gene expression refers to how much a particular gene is active, leading to the production of the protein it codes for.
  • They utilized a technique known as reverse transcriptase real-time quantitative PCR to evaluate this gene expression. This technique allows them to measure the amount of specific RNA (the product of gene expression) present in the cells.
  • The research revealed that the MSCs expressed transcripts for several markers including CD13, CD29, CD44, CD49d, CD73, CD90, CD105, CD106, CD146, and CD166.
  • The study noted identifiable differences in gene expression levels between the MSCs derived from bone marrow and those from adipose tissue, especially for CD44, CD90, CD29, and CD34.
  • In both types of cells, the researchers found no markers for CD45 and CD31, suggesting these are not characteristic of equine MSCs or they were not expressed at this particular stage of cell growth.

Relationship between Horse and Human MSCs

  • Although the study’s focus was on equine MSCs, it also made comparisons with human MSCs, observing similarities in their phenotype profiles. That is, the basic biological characteristics of the cells, as revealed by their surface markers, were much alike.
  • However, the researchers noted specific differences in certain surface antigens. More research would be needed to specify and understand these differences fully.

In conclusion, the study provides detailed insights into the biological characteristics of equine MSCs, paving the way for further research into their potential applications, such as in the field of regenerative medicine.

Cite This Article

APA
Ranera B, Lyahyai J, Romero A, Vázquez FJ, Remacha AR, Bernal ML, Zaragoza P, Rodellar C, Martín-Burriel I. (2011). Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue. Vet Immunol Immunopathol, 144(1-2), 147-154. https://doi.org/10.1016/j.vetimm.2011.06.033

Publication

Veterinary immunology and immunopathology
ISSN: 1873-2534
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 144
Issue: 1-2
Pages: 147-154

Researcher Affiliations

Ranera, Beatriz
  • Laboratorio de Genética Bioquímica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza, 50013, Zaragoza, Spain.
Lyahyai, Jaber
    Romero, Antonio
      Vázquez, Francisco José
        Remacha, Ana Rosa
          Bernal, María Luisa
            Zaragoza, Pilar
              Rodellar, Clementina
                Martín-Burriel, Inmaculada

                  MeSH Terms

                  • Adipose Tissue / cytology
                  • Adipose Tissue / immunology
                  • Animals
                  • Antigens, Surface / immunology
                  • Bone Marrow Cells / immunology
                  • Flow Cytometry / veterinary
                  • Gene Expression Profiling / veterinary
                  • Horses / genetics
                  • Horses / immunology
                  • Immunophenotyping / veterinary
                  • Integrin beta1 / immunology
                  • Mesenchymal Stem Cells / immunology
                  • Real-Time Polymerase Chain Reaction / veterinary
                  • Thy-1 Antigens / immunology

                  Citations

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