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Home / Equine Research Bank / J Biol Eng / Improved expansion of equine cord blood derived mesenchymal ...
Journal of biological engineering2019; 13; 25; doi: 10.1186/s13036-019-0153-8

Improved expansion of equine cord blood derived mesenchymal stromal cells by using microcarriers in stirred suspension bioreactors.

Abstract: Equine mesenchymal stromal cells (MSCs) are increasingly investigated for their clinical therapeutic utility. Such cell-based treatments can require cell numbers in the millions or billions, with conventional expansion methods using static T-flasks typically inefficient in achieving these cell numbers. Equine cord blood-derived MSCs (eCB-MSCs), are promising cell candidates owing to their capacity for chondrogenic differentiation and immunomodulation. Expansion of eCB-MSCs in stirred suspension bioreactors with microcarriers as an attachment surface has the potential to generate clinically relevant numbers of cells while decreasing cost, time and labour requirements and increasing reproducibility and yield when compared to static expansion. As eCB-MSCs have not yet been expanded in stirred suspension bioreactors, a robust protocol was required to expand these cells using this method. This study outlines the development of an expansion bioprocess, detailing the inoculation phase, expansion phase, and harvesting phase, followed by phenotypic and trilineage differentiation characterization of two eCB-MSC donors. The process achieved maximum cell densities up to 75,000 cells/cm corresponding to 40 million cells in a 100 mL bioreactor, with a harvesting efficiency of up to 80%, corresponding to a yield of 32 million cells from a 100 mL bioreactor. When compared to cells grown in static T-flasks, bioreactor-expanded eCB-MSC cultures did not change in surface marker expression or trilineage differentiation capacity. This indicates that the bioreactor expansion process yields large quantities of eCB-MSCs with similar characteristics to conventionally grown eCB-MSCs.
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Publication Date: 2019-03-21 PubMed ID: 30949237PubMed Central: PMC6429778DOI: 10.1186/s13036-019-0153-8Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores a more efficient way of expanding equine cord blood-derived mesenchymal stromal cells (eCB-MSCs) by utilizing stirred suspension bioreactors with microcarriers, as opposed to conventional methods. The optimum efficiency and yield achieved with this method suggest it’s a viable approach, providing clinically relevant quantities of cells with similar properties to those expanded traditionally.

Objective of the Research

  • The goal of this research was to devise a robust protocol for the expansion of eCB-MSCs using stirred suspension bioreactors with microcarriers. Traditional methods involving the use of static T-flasks for cell expansion tend to be inefficient, making it hard to produce the high quantities of cells necessary for cell-based therapies.

Methodology Employed

  • The researchers developed a bioprocess for cell expansion, covering the inoculation phase (introduction of the cells), the expansion phase (growth of the cells), and the harvesting phase (collection of the cells).
  • This bioprocess was used on two eCB-MSC donors, with the cell densities, harvesting efficiency, and the characteristics of the expanded cells being carefully monitored.

Results and Findings of the Study

  • The expansion process achieved a maximum cell density of 75,000 cells/cm, equivalent to 40 million cells in a 100 mL bioreactor.
  • The harvesting process saw an efficiency of up to 80%, leading to a yield of 32 million cells from a 100 mL bioreactor.
  • The characteristics of the cells expanded in the bioreactor, as it relates to surface marker expression or trilineage differentiation capacity, showed no differences in comparison to cells grown in conventional static T-flasks.

Ideal Implications and Uses of the Research

  • This efficient bioprocess for eCB-MSC production could, in the future, hold a significant role in therapeutic interventions that require large-volume production of MSCs.
  • Since the expanded cells retained their key characteristics, the bioprocess doesn’t appear to compromise the therapeutic potential of the cells.
  • The use of this method could reduce the cost, time, and labor involved in cell expansion, while also improving reproducibility and yield, therefore increasing the feasibility of MSC-derived therapies.

Cite This Article

APA
Roberts EL, Dang T, Lepage SIM, Alizadeh AH, Walsh T, Koch TG, Kallos MS. (2019). Improved expansion of equine cord blood derived mesenchymal stromal cells by using microcarriers in stirred suspension bioreactors. J Biol Eng, 13, 25. https://doi.org/10.1186/s13036-019-0153-8

Publication

Journal of biological engineering
ISSN: 1754-1611
NlmUniqueID: 101306640
Country: England
Language: English
Volume: 13
Pages: 25
PII: 25

Researcher Affiliations

Roberts, Erin L
  • 1Pharmaceutical Production Research Facility, Schulich School of Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.
  • 2Biomedical Engineering Graduate Program, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.
Dang, Tiffany
  • 1Pharmaceutical Production Research Facility, Schulich School of Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.
  • 2Biomedical Engineering Graduate Program, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.
Lepage, Sarah I M
  • 3Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Gordon St, Guelph, ON N1G 2W1 Canada.
Alizadeh, Amir Hamed
  • 3Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Gordon St, Guelph, ON N1G 2W1 Canada.
Walsh, Tylor
  • 1Pharmaceutical Production Research Facility, Schulich School of Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.
  • 2Biomedical Engineering Graduate Program, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.
Koch, Thomas G
  • 3Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Gordon St, Guelph, ON N1G 2W1 Canada.
Kallos, Michael S
  • 1Pharmaceutical Production Research Facility, Schulich School of Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.
  • 2Biomedical Engineering Graduate Program, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.
  • 4Department of Chemical and Petroleum Engineering, Schulich School of Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4 Canada.

Conflict of Interest Statement

Not applicable.Not applicable.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

This article includes 56 references
  1. Equine Canada. 2010 Canadian Horse Industry Profile Study; 2010. http://equinecanada.ca/industry/index.php?option=com_content&view=section&id=103&Itemid=559&lang=en.
  2. Koch TG, Heerkens T, Thomsen PD, Betts DH. Isolation of mesenchymal stem cells from equine umbilical cord blood.. BMC Biotechnol 2007 May 30;7:26.
    doi: 10.1186/1472-6750-7-26pmc: PMC1904213pubmed: 17537254google scholar: lookup
  3. Godwin EE, Young NJ, Dudhia J, Beamish IC, Smith RK. Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon.. Equine Vet J 2012 Jan;44(1):25-32.
    doi: 10.1111/j.2042-3306.2011.00363.xpubmed: 21615465google scholar: lookup
  4. Dyson SJ. Medical management of superficial digital flexor tendonitis: a comparative study in 219 horses (1992-2000).. Equine Vet J 2004 Jul;36(5):415-9.
    doi: 10.2746/0425164044868422pubmed: 15253082google scholar: lookup
  5. Smith RK. Mesenchymal stem cell therapy for equine tendinopathy.. Disabil Rehabil 2008;30(20-22):1752-8.
    doi: 10.1080/09638280701788241pubmed: 18608378google scholar: lookup
  6. Carrade DD, Lame MW, Kent MS, Clark KC, Walker NJ, Borjesson DL. Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().. Cell Med 2012;4(1):1-11.
    doi: 10.3727/215517912X647217.Comparativepmc: PMC3495591pubmed: 23152950google scholar: lookup
  7. Cunha B, Aguiar T, Carvalho SB, Silva MM, Gomes RA, Carrondo MJT, Gomes-Alves P, Peixoto C, Serra M, Alves PM. Bioprocess integration for human mesenchymal stem cells: From up to downstream processing scale-up to cell proteome characterization.. J Biotechnol 2017 Apr 20;248:87-98.
    doi: 10.1016/j.jbiotec.2017.01.014pubmed: 28174039google scholar: lookup
  8. Rafiq QA, Coopman K, Nienow AW, Hewitt CJ. Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors.. Biotechnol J 2016 Mar;11(4):473-86.
    pmc: PMC4991290pubmed: 26632496doi: 10.1002/biot.201400862google scholar: lookup
  9. Petry F, Smith JR, Leber J, Salzig D, Czermak P, Weiss ML. Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use.. Stem Cells Int 2016;2016:4834616.
    pmc: PMC4761675pubmed: 26977155doi: 10.1155/2016/4834616google scholar: lookup
  10. Schop D, van Dijkhuizen-Radersma R, Borgart E, Janssen FW, Rozemuller H, Prins HJ, de Bruijn JD. Expansion of human mesenchymal stromal cells on microcarriers: growth and metabolism.. J Tissue Eng Regen Med 2010 Feb;4(2):131-40.
    doi: 10.1002/termpubmed: 19842106google scholar: lookup
  11. Goh TK, Zhang ZY, Chen AK, Reuveny S, Choolani M, Chan JK, Oh SK. Microcarrier culture for efficient expansion and osteogenic differentiation of human fetal mesenchymal stem cells.. Biores Open Access 2013 Apr;2(2):84-97.
    pmc: PMC3620494pubmed: 23593561doi: 10.1089/biores.2013.0001google scholar: lookup
  12. Martin Y, Eldardiri M, Lawrence-Watt DJ, Sharpe JR. Microcarriers and their potential in tissue regeneration.. Tissue Eng Part B Rev 2011 Feb;17(1):71-80.
    doi: 10.1089/ten.teb.2010.0559pubmed: 21083436google scholar: lookup
  13. Rafiq QA, Coopman K, Hewitt CJ. Scale-up of human mesenchymal stem cell culture : current technologies and future challenges. Curr Opin Chem Eng 2014;2(1):8–16.
    doi: 10.1016/j.coche.2013.01.005google scholar: lookup
  14. Balint R, Richardson SM, Cartmell SH. Low-density subculture: a technical note on the importance of avoiding cell-to-cell contact during mesenchymal stromal cell expansion.. J Tissue Eng Regen Med 2015 Oct;9(10):1200-3.
    pmc: PMC4858810pubmed: 26153119doi: 10.1002/term.2051google scholar: lookup
  15. Sun LY, Hsieh DK, Syu WS, Li YS, Chiu HT, Chiou TW. Cell proliferation of human bone marrow mesenchymal stem cells on biodegradable microcarriers enhances in vitro differentiation potential.. Cell Prolif 2010 Oct;43(5):445-56.
    doi: 10.1111/j.1365-2184.2010.00694.xpmc: PMC6496285pubmed: 20887551google scholar: lookup
  16. Li P, Liu F, Wu C, Jiang W, Zhao G, Liu L, Bai T, Wang L, Jiang Y, Guo L, Qi X, Kou J, Fan R, Hao D, Lan S, Li Y, Liu JY. Feasibility of human hair follicle-derived mesenchymal stem cells/CultiSpher(®)-G constructs in regenerative medicine.. Cell Tissue Res 2015 Oct;362(1):69-86.
    doi: 10.1007/s00441-015-2182-zpubmed: 25948482google scholar: lookup
  17. Shekaran A, Lam A, Sim E, Jialing L, Jian L, Wen JT, Chan JK, Choolani M, Reuveny S, Birch W, Oh S. Biodegradable ECM-coated PCL microcarriers support scalable human early MSC expansion and in vivo bone formation.. Cytotherapy 2016 Oct;18(10):1332-44.
    doi: 10.1016/j.jcyt.2016.06.016pubmed: 27503763google scholar: lookup
  18. Lam AT, Li J, Toh JP, Sim EJ, Chen AK, Chan JK, Choolani M, Reuveny S, Birch WR, Oh SK. Biodegradable poly-ε-caprolactone microcarriers for efficient production of human mesenchymal stromal cells and secreted cytokines in batch and fed-batch bioreactors.. Cytotherapy 2017 Mar;19(3):419-432.
    doi: 10.1016/j.jcyt.2016.11.009pubmed: 28017598google scholar: lookup
  19. Lin YM, Lee J, Lim JFY, Choolani M, Chan JKY, Reuveny S, Oh SKW. Critical attributes of human early mesenchymal stromal cell-laden microcarrier constructs for improved chondrogenic differentiation.. Stem Cell Res Ther 2017 May 8;8(1):93.
    doi: 10.1186/s13287-017-0538-xpmc: PMC5421335pubmed: 28482913google scholar: lookup
  20. Ibrahim AM, Elgharabawi NM, Makhlouf MM, Ibrahim OY. Chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in vitro.. Microsc Res Tech 2015 Aug;78(8):667-75.
    doi: 10.1002/jemt.22520pubmed: 26096638google scholar: lookup
  21. Van Pham P, Phan NK. Production of good manufacturing practice-grade human umbilical cord blood-derived mesenchymal stem cells for therapeutic use.. Methods Mol Biol 2015;1283:73-85.
    doi: 10.1007/7651_2014_125pubmed: 25239529google scholar: lookup
  22. Peters R, Wolf MJ, van den Broek M, Nuvolone M, Dannenmann S, Stieger B, Rapold R, Konrad D, Rubin A, Bertino JR, Aguzzi A, Heikenwalder M, Knuth AK. Efficient generation of multipotent mesenchymal stem cells from umbilical cord blood in stroma-free liquid culture.. PLoS One 2010 Dec 30;5(12):e15689.
    doi: 10.1371/journal.pone.0015689pmc: PMC3012708pubmed: 21209896google scholar: lookup
  23. Jossen V, Schirmer C, Mostafa Sindi D, Eibl R, Kraume M, Pörtner R, Eibl D. Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes.. Stem Cells Int 2016;2016:4760414.
    pmc: PMC4766353pubmed: 26981131doi: 10.1155/2016/4760414google scholar: lookup
  24. Yuan Y, Kallos MS, Hunter C, Sen A. Improved expansion of human bone marrow-derived mesenchymal stem cells in microcarrier-based suspension culture.. J Tissue Eng Regen Med 2014 Mar;8(3):210-25.
    doi: 10.1002/term.1515pubmed: 22689330google scholar: lookup
  25. Takahashi I, Sato K, Mera H, Wakitani S, Takagi M. Effects of agitation rate on aggregation during beads-to-beads subcultivation of microcarrier culture of human mesenchymal stem cells.. Cytotechnology 2017 Jun;69(3):503-509.
    pmc: PMC5461241pubmed: 27352111doi: 10.1007/s10616-016-9999-5google scholar: lookup
  26. Koch TG, Thomsen PD, Betts DH. Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells.. Cytotherapy 2009;11(4):443-7.
    doi: 10.1080/14653240902887259pubmed: 19513899google scholar: lookup
  27. Co C, Vickaryous MK, Koch TG. Membrane culture and reduced oxygen tension enhances cartilage matrix formation from equine cord blood mesenchymal stromal cells in vitro.. Osteoarthritis Cartilage 2014 Mar;22(3):472-80.
    pubmed: 24418676doi: 10.1016/j.joca.2013.12.021google scholar: lookup
  28. Caruso SR, Orellana MD, Mizukami A, Fernandes TR, Fontes AM, Suazo CA, Oliveira VC, Covas DT, Swiech K. Growth and functional harvesting of human mesenchymal stromal cells cultured on a microcarrier-based system.. Biotechnol Prog 2014 Jul-Aug;30(4):889-95.
    doi: 10.1002/btpr.1886pubmed: 24574042google scholar: lookup
  29. Yang HS, Jeon O, Bhang SH, Lee SH, Kim BS. Suspension culture of mammalian cells using thermosensitive microcarrier that allows cell detachment without proteolytic enzyme treatment.. Cell Transplant 2010;19(9):1123-32.
    pubmed: 20719079doi: 10.3727/096368910x516664google scholar: lookup
  30. Lin YM, Lim JF, Lee J, Choolani M, Chan JK, Reuveny S, Oh SK. Expansion in microcarrier-spinner cultures improves the chondrogenic potential of human early mesenchymal stromal cells.. Cytotherapy 2016 Jun;18(6):740-53.
    doi: 10.1016/j.jcyt.2016.03.293pubmed: 27173750google scholar: lookup
  31. Hewitt CJ, Lee K, Nienow AW, Thomas RJ, Smith M, Thomas CR. Expansion of human mesenchymal stem cells on microcarriers.. Biotechnol Lett 2011 Nov;33(11):2325-35.
    pubmed: 21769648doi: 10.1007/s10529-011-0695-4google scholar: lookup
  32. Ma T, Tsai AC, Liu Y. Biomanufacturing of human mesenchymal stem cells in cell therapy: influence of microenvironment on scalable expansion in bioreactors. Biochem Eng J 2016;108:44–50.
    doi: 10.1016/j.bej.2015.07.014google scholar: lookup
  33. Kamura S, Matsumoto Y, Fukushi JI, Fujiwara T, Iida K, Okada Y, Iwamoto Y. Basic fibroblast growth factor in the bone microenvironment enhances cell motility and invasion of Ewing's sarcoma family of tumours by activating the FGFR1-PI3K-Rac1 pathway.. Br J Cancer 2010 Jul 27;103(3):370-81.
    doi: 10.1038/sj.bjc.6605775pmc: PMC2920026pubmed: 20606682google scholar: lookup
  34. Tsutsumi S, Shimazu A, Miyazaki K, Pan H, Koike C, Yoshida E, Takagishi K, Kato Y. Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF.. Biochem Biophys Res Commun 2001 Oct 26;288(2):413-9.
    doi: 10.1006/bbrc.2001.5777pubmed: 11606058google scholar: lookup
  35. Korc M, Friesel RE. The role of fibroblast growth factors in tumor growth.. Curr Cancer Drug Targets 2009 Aug;9(5):639-51.
    doi: 10.2174/156800909789057006pmc: PMC3664927pubmed: 19508171google scholar: lookup
  36. Santos Fd, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, Campbell AM, Boucher S, Vemuri MC, Silva CL, Cabral JM. Toward a clinical-grade expansion of mesenchymal stem cells from human sources: a microcarrier-based culture system under xeno-free conditions.. Tissue Eng Part C Methods 2011 Dec;17(12):1201-10.
    doi: 10.1089/ten.tec.2011.0255pmc: PMC3226421pubmed: 21895491google scholar: lookup
  37. Schop D, Janssen FW, van Rijn LD, Fernandes H, Bloem RM, de Bruijn JD, van Dijkhuizen-Radersma R. Growth, metabolism, and growth inhibitors of mesenchymal stem cells.. Tissue Eng Part A 2009 Aug;15(8):1877-86.
    pubmed: 19196147doi: 10.1089/ten.tea.2008.0345google scholar: lookup
  38. Pattappa G, Heywood HK, de Bruijn JD, Lee DA. The metabolism of human mesenchymal stem cells during proliferation and differentiation.. J Cell Physiol 2011 Oct;226(10):2562-70.
    doi: 10.1002/jcp.22605pubmed: 21792913google scholar: lookup
  39. Liu Y, Ma T. Metabolic regulation of mesenchymal stem cell in expansion and therapeutic application.. Biotechnol Prog 2015 Mar-Apr;31(2):468-81.
    doi: 10.1002/btpr.2034pubmed: 25504836google scholar: lookup
  40. Hu C, Fan L, Cen P, Chen E, Jiang Z, Li L. Energy Metabolism Plays a Critical Role in Stem Cell Maintenance and Differentiation.. Int J Mol Sci 2016 Feb 18;17(2):253.
    pmc: PMC4783982pubmed: 26901195doi: 10.3390/ijms17020253google scholar: lookup
  41. White CR, Frangos JA. The shear stress of it all: the cell membrane and mechanochemical transduction.. Philos Trans R Soc Lond B Biol Sci 2007 Aug 29;362(1484):1459-67.
    pmc: PMC2440408pubmed: 17569643doi: 10.1098/rstb.2007.2128google scholar: lookup
  42. Stolberg S, McCloskey KE. Can shear stress direct stem cell fate?. Biotechnol Prog 2009 Jan-Feb;25(1):10-9.
    pubmed: 19197983doi: 10.1002/btpr.124google scholar: lookup
  43. Fok EY, Zandstra PW. Shear-controlled single-step mouse embryonic stem cell expansion and embryoid body-based differentiation.. Stem Cells 2005 Oct;23(9):1333-42.
    doi: 10.1634/stemcells.2005-0112pubmed: 16081660google scholar: lookup
  44. Yourek G, McCormick SM, Mao JJ, Reilly GC. Shear stress induces osteogenic differentiation of human mesenchymal stem cells.. Regen Med 2010 Sep;5(5):713-24.
    doi: 10.2217/rme.10.60pmc: PMC4093787pubmed: 20868327google scholar: lookup
  45. Bassaneze V, Barauna VG, Lavini-Ramos C, Kalil J, Schettert IT, Miyakawa AA, Krieger JE. Shear stress induces nitric oxide-mediated vascular endothelial growth factor production in human adipose tissue mesenchymal stem cells.. Stem Cells Dev 2010 Mar;19(3):371-8.
    doi: 10.1089/scd.2009.0195pubmed: 19754225google scholar: lookup
  46. Weber C, Pohl S, Pörtner R, Wallrapp C, Kassem M, Geigle P, Czermak P. Expansion and Harvesting of hMSC-TERT.. Open Biomed Eng J 2007 Sep 7;1:38-46.
    pmc: PMC2701074pubmed: 19662126doi: 10.2174/1874120700701010038google scholar: lookup
  47. Bonab MM, Alimoghaddam K, Talebian F, Ghaffari SH, Ghavamzadeh A, Nikbin B. Aging of mesenchymal stem cell in vitro. 2006. pp. 1–7.
  48. Heathman TRJ, Rafiq QA, Chan AKC. Characterization of human mesenchymal stem cells from multiple donors and the implications for large scale bioprocess development. Biochem Eng J 2016;108:14–23.
    doi: 10.1016/j.bej.2015.06.018google scholar: lookup
  49. Phinney DG, Kopen G, Righter W, Webster S, Tremain N, Prockop DJ. Donor variation in the growth properties and osteogenic potential of human marrow stromal cells.. J Cell Biochem 1999 Dec 1;75(3):424-36.
    pubmed: 10536366
  50. Carter-Arnold JL, Neilsen NL, Amelse LL, Odoi A, Dhar MS. In vitro analysis of equine, bone marrow-derived mesenchymal stem cells demonstrates differences within age- and gender-matched horses.. Equine Vet J 2014 Sep;46(5):589-95.
    pubmed: 23855680doi: 10.1111/evj.12142google scholar: lookup
  51. Lepage SIM. Generation of equine osteochondral constructs using mesenchyaml stromal cells. 2016.
  52. Lawson T, Kehoe DE, Schnitzler AC. Process development for expansion of human mesenchymal stromal cells in a 50 L single-use stirred tank bioreactor. Biochem Eng J 2017;120:49–62.
    doi: 10.1016/J.BEJ.2016.11.020google scholar: lookup
  53. Tessier L, Bienzle D, Williams LB, Koch TG. Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.. PLoS One 2015;10(4):e0122954.
    doi: 10.1371/journal.pone.0122954pmc: PMC4406608pubmed: 25902064google scholar: lookup
  54. Ranera B, Lyahyai J, Romero A, Vázquez FJ, Remacha AR, Bernal ML, Zaragoza P, Rodellar C, Martín-Burriel I. Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue.. Vet Immunol Immunopathol 2011 Nov 15;144(1-2):147-54.
    doi: 10.1016/j.vetimm.2011.06.033pubmed: 21782255google scholar: lookup
  55. De Schauwer C, Piepers S, Van de Walle GR, Demeyere K, Hoogewijs MK, Govaere JL, Braeckmans K, Van Soom A, Meyer E. In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.. Cytometry A 2012 Apr;81(4):312-23.
    doi: 10.1002/cyto.a.22026pubmed: 22411893google scholar: lookup
  56. De Schauwer C, van de Walle GR, Piepers S, Hoogewijs MK, Govaere JL, Meyer E, van Soom A. Successful isolation of equine mesenchymal stromal cells from cryopreserved umbilical cord blood-derived mononuclear cell fractions.. Equine Vet J 2013 Jul;45(4):518-22.
    doi: 10.1111/evj.12003pubmed: 23206252google scholar: lookup

Citations

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