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Home / Equine Research Bank / Vet Res Commun / Increased apoptosis of CD4 and CD8 T lymphocytes in the airw...
Veterinary research communications2011; 35(7); 447-456; doi: 10.1007/s11259-011-9482-x

Increased apoptosis of CD4 and CD8 T lymphocytes in the airways of horses with recurrent airway obstruction.

Abstract: Recurrent airway obstruction (RAO, also known as equine heaves) is an inflammatory condition similar to human asthma caused by exposure of susceptible horses to poorly ventilated stable environments. The disease is characterized by neutrophilic airway inflammation, mucus hypersecretion and reversible bronchoconstriction. This inflammatory process is mediated by several factors, including antibodies, cytokines, resident cells of the airway and inflammatory cellular components that arrive in the respiratory tract. An increasing body of evidence has lent support to the concept that a dysregulation of T cell apoptosis may play a central role in the development of airway inflammation and the associated asthma. Therefore, the aim of this study was to investigate early and late apoptosis of CD4 and CD8 T cell subpopulations obtained from the airways of acute RAO-positive animals after exposure to hay/straw. The percentages of CD4 and CD8 T cells and their associated frequencies of apoptosis were quantified using flow cytometry. Hay/straw exposure induced clinical airway obstruction, airway neutrophilia and increased airway mucus production in RAO-positive horses. In addition, allergen exposure increased the percentage of CD4 T cells in RAO-positive horses as well as the frequency of early and late apoptosis in both CD4 and CD8 lymphocyte subpopulations. These results suggest that the higher frequency of lymphocyte apoptosis may play a role in disease progression of horses afflicted with RAO and may partially explain the characteristic remission of this pathological condition once the allergen source is removed. However, further studies are needed to clarify the role of T cell apoptosis in RAO-affected horses.
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Publication Date: 2011-05-19 PubMed ID: 21594641DOI: 10.1007/s11259-011-9482-xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study focused on understanding the role of increased apoptosis of CD4 and CD8 T lymphocytes or T cells in horses with recurrent airway obstruction (RAO). The study has seen increased apoptosis in these T cells after exposure to hay/straw, suggesting this could play a role in the disease progression of RAO.

Understanding Recurrent Airway Obstruction (RAO)

  • RAO, also referred to as equine heaves, is an inflammatory condition in horses, similar to human asthma. It mainly occurs due to exposure to poorly ventilated stable environments.
  • The disease results in neutrophilic airway inflammation, mucus hypersecretion, and reversible bronchoconstriction. Several factors contribute to this inflammatory process, including antibodies, cytokines, airway resident cells, and inflammatory cellular components that arrive in the respiratory tract.

Role of T Cell Apoptosis in RAO

  • An excessive apoptosis or programmed cell death of T cells is thought to be a central factor in airway inflammation and thus, in conditions like RAO and asthma. The study aimed to explore this concept further.

Study Observations

  • The study used flow cytometry to quantify the percentages of CD4 and CD8 T cells and the associated frequencies of their apoptosis. This was done on T cell subpopulations from the airways of acute RAO-positive animals following exposure to hay/straw.
  • Exposure to hay/straw resulted in clinical airway obstruction, airway neutrophilia (a high number of neutrophils, a type of white blood cell), and increased mucus production in the airways of RAO-positive horses.
  • Following allergen exposure, there was an increase in the percentage of CD4 T cells as well as an increase in the frequency of early and late apoptosis in both CD4 and CD8 lymphocyte subpopulations.

Implications of the Findings

  • The above results suggest that greater lymphocyte apoptosis might contribute to the progression of RAO in horses. This might also partially explain the characteristic remission of RAO once the source of the allergen is eliminated.
  • Nonetheless, further research is required to fully understand the role of T cell apoptosis in RAO-affected horses.

Cite This Article

APA
Moran G, Buechner-Maxwell VA, Folch H, Henriquez C, Galecio JS, Perez B, Carrasco C, Barria M. (2011). Increased apoptosis of CD4 and CD8 T lymphocytes in the airways of horses with recurrent airway obstruction. Vet Res Commun, 35(7), 447-456. https://doi.org/10.1007/s11259-011-9482-x

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 35
Issue: 7
Pages: 447-456

Researcher Affiliations

Moran, Gabriel
  • Department of Pharmacology, Faculty of Veterinary Science, Universidad Austral de Chile, 567, Valdivia, Chile. gmoran@uach.cl
Buechner-Maxwell, Virginia A
    Folch, Hugo
      Henriquez, Claudio
        Galecio, Juan S
          Perez, Barbara
            Carrasco, Cristian
              Barria, Miguel

                MeSH Terms

                • Airway Obstruction / immunology
                • Airway Obstruction / pathology
                • Airway Obstruction / veterinary
                • Animals
                • Apoptosis
                • Bronchoalveolar Lavage Fluid / immunology
                • CD4-Positive T-Lymphocytes / cytology
                • CD4-Positive T-Lymphocytes / immunology
                • CD8-Positive T-Lymphocytes / cytology
                • CD8-Positive T-Lymphocytes / immunology
                • Female
                • Flow Cytometry / veterinary
                • Horse Diseases / immunology
                • Horse Diseases / pathology
                • Horses
                • Poaceae
                • Respiratory System / immunology

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                Citations

                This article has been cited 6 times.
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