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Scientific reports2021; 11(1); 14278; doi: 10.1038/s41598-021-93468-2

Integrative biology defines novel biomarkers of resistance to strongylid infection in horses.

Abstract: The widespread failure of anthelmintic drugs against nematodes of veterinary interest requires novel control strategies. Selective treatment of the most susceptible individuals could reduce drug selection pressure but requires appropriate biomarkers of the intrinsic susceptibility potential. To date, this has been missing in livestock species. Here, we selected Welsh ponies with divergent intrinsic susceptibility (measured by their egg excretion levels) to cyathostomin infection and found that their divergence was sustained across a 10-year time window. Using this unique set of individuals, we monitored variations in their blood cell populations, plasma metabolites and faecal microbiota over a grazing season to isolate core differences between their respective responses under worm-free or natural infection conditions. Our analyses identified the concomitant rise in plasma phenylalanine level and faecal Prevotella abundance and the reduction in circulating monocyte counts as biomarkers of the need for drug treatment (egg excretion above 200 eggs/g). This biological signal was replicated in other independent populations. We also unravelled an immunometabolic network encompassing plasma beta-hydroxybutyrate level, short-chain fatty acid producing bacteria and circulating neutrophils that forms the discriminant baseline between susceptible and resistant individuals. Altogether our observations open new perspectives on the susceptibility of equids to strongylid infection and leave scope for both new biomarkers of infection and nutritional intervention.
© 2021. The Author(s).
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Publication Date: 2021-07-12 PubMed ID: 34253752PubMed Central: PMC8275762DOI: 10.1038/s41598-021-93468-2Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates new biological markers, specific changes in blood cells, plasma metabolite levels, and fecal microbiota, that could potentially predict horses’ resistance or susceptibility to a type of worm infection. These findings offer new insights and suggest potential novel interventions for managing worm infection in horses.

Introduction and Objectives

  • This research aims to find biomarkers that indicate innate susceptibility to strongylid worm infections in horses. These worms are a common and significant health issue in horses, and a growing resistance to commonly applied treatments has triggered the need for new intervention strategies.
  • The key objective is to reduce the use of anthelmintic drugs, which currently play a key role in managing these infections, but widespread usage has led to drug resistance amongst worms.

Methodology

  • The researchers selectively isolated Welsh ponies with evident differences in susceptibility to cyathostomin infection, determined by their egg excretion levels and monitored over a 10-year period.
  • The variations between these selected horses, in terms of blood cell populations, plasma metabolites, and faecal microbiota, were monitored over a grazing season. The aim was to observe core differences in response when exposed to worm-free or naturally infected conditions.

Key Findings

  • The study identified three key indicators for the need for drug treatment in horses: a simultaneous increase in plasma phenylalanine level and faecal Prevotella abundance, and a decrease in circulating monocyte counts. These findings were apparent when egg excretion reached above 200 eggs/g, suggesting that these markers significantly correlate with the severity of infection.
  • The scientists discovered an immunometabolic network connecting plasma beta-hydroxybutyrate level, short-chain fatty acid producing bacteria and circulating neutrophils. This network was found to strongly discriminate between susceptible and resistant individuals.

Implications and Future Directions

  • The findings add a new dimension to knowledge on equine susceptibility to strongylid infection. Simultaneously, they provide potential new biomarkers to monitor and manage infection levels and explore the potential of nutritional interventions.
  • The scope of these findings implies that understanding the host’s responses and identifying biomarkers can lead to more targeted treatments, promoting animal health and reducing the imposition or overuse of anthelmintic drugs, thus combating drug resistance issues in nematodes.

Cite This Article

APA
Sallé G, Canlet C, Cortet J, Koch C, Malsa J, Reigner F, Riou M, Perrot N, Blanchard A, Mach N. (2021). Integrative biology defines novel biomarkers of resistance to strongylid infection in horses. Sci Rep, 11(1), 14278. https://doi.org/10.1038/s41598-021-93468-2

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 11
Issue: 1
Pages: 14278
PII: 14278

Researcher Affiliations

Sallé, Guillaume
  • INRAE, Université de Tours, UMR 1282 Infectiologie et Santé Publique, 37380, Nouzilly, France. Guillaume.Salle@inrae.fr.
Canlet, Cécile
  • INRAE, Université de Toulouse, ENVT, INP-Purpan, UPS, UMR 1331 Toxalim, 31027, Toulouse, France.
Cortet, Jacques
  • INRAE, Université de Tours, UMR 1282 Infectiologie et Santé Publique, 37380, Nouzilly, France.
Koch, Christine
  • INRAE, Université de Tours, UMR 1282 Infectiologie et Santé Publique, 37380, Nouzilly, France.
Malsa, Joshua
  • INRAE, Université de Tours, UMR 1282 Infectiologie et Santé Publique, 37380, Nouzilly, France.
Reigner, Fabrice
  • INRAE, UE 1297 Physiologie Animale de l'Orfrasière, 37380, Nouzilly, France.
Riou, Mickaël
  • INRAE, UE 1277 Plateforme d'Infectiologie Expérimentale, 37380, Nouzilly, France.
Perrot, Noémie
  • INRAE, UE 1277 Plateforme d'Infectiologie Expérimentale, 37380, Nouzilly, France.
Blanchard, Alexandra
  • Pancosma, Rolle, Switzerland.
Mach, Núria
  • INRAE, Université Paris-Saclay, AgroParisTech, Génétique Animale et Biologie Intégrative, 78350, Jouy-en-Josas, France.

MeSH Terms

  • 3-Hydroxybutyric Acid / blood
  • Animals
  • Anthelmintics / therapeutic use
  • Biology / methods
  • Biomarkers / metabolism
  • Discriminant Analysis
  • Feces
  • Horse Diseases / parasitology
  • Horses
  • Magnetic Resonance Spectroscopy
  • Metabolomics
  • Nematoda
  • Parasite Egg Count / veterinary
  • Phenylalanine / blood
  • Seasons

Conflict of Interest Statement

The authors declare no competing interests.

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Citations

This article has been cited 3 times.
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