Integrin alpha-v/beta3 expression in equine lungs and jejunum.
Abstract: Integrin alpha-v/beta3 (αvβ3) recognizes arginine-glycine-aspartic acid (RGD) sequences and has important functions in cell adhesion, signaling, and survival. However, the expression of integrin αvβ3 in the equine lungs and jejunum is not well understood. The objective of this study was to explore the hitherto unknown expression of integrin αvβ3 in the lungs and jejuna of the horse using light and electron immunocytochemistry. Immunohistochemistry showed integrin αvβ3 on the epithelium, the immune cells in Peyer's patches, the smooth muscle, and the endothelium of equine jejuna. In equine lungs, we recognized integrin αvβ3 on the endothelium of blood vessels, the alveolar septa, the bronchial lymph nodes, and the cartilages, although the expression of integrin αvβ3 was weak on the epithelium of bronchioles. In conclusion, these are the first data to show the expression of integrin αvβ3 in equine lungs and jejuna. L’intégrine alpha-v/beta3 (αvβ3) reconnaît les séquences arginine-glycine-acide aspartique (RGD) et a d’importantes fonctions dans l’adhésion cellulaire, la signalisation et la survie. Toutefois, l’expression de l’intégrine αvβ3 dans les poumons et le jéjunum équins n’est pas bien comprise. L’objectif de la présente étude était d’explorer l’expression inconnue jusqu’à ce jour de l’intégrine αvβ3 dans les poumons et le jéjunum du cheval en utilisant l’immunohistochimie photonique et électronique. L’immunohistochimie a montré l’intégrine αvβ3 sur l’épithélium, les cellules immunitaires des plaques de Peyer, le muscle lisse et l’endothélium du jéjunum équin. Dans les poumons équins, nous avons reconnu l’intégrine αvβ3 sur l’endothélium des vaisseaux sanguins, les septas alvéolaires, les noeuds lymphatique bronchiaux et les cartilages, quoique l’expression de l’intégrine αvβ3 était faible sur l’épithélium des bronchioles. En conclusion, ceci représente les premiers résultats qui démontrent l’expression de l’intégrine αvβ3 dans les poumons et le jéjunum équins.(Traduit par Docteur Serge Messier).
Copyright and/or publishing rights held by the Canadian Veterinary Medical Association.
Publication Date: 2020-10-06 PubMed ID: 33012972PubMed Central: PMC7491005
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- Journal Article
Summary
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The research article investigates the expression of Integrin alpha-v/beta3 (αvβ3), a protein important for cell adhesion, signaling, and survival, in the lungs and jejunum of horses. This marks the first study to show such expression in these particular equine organs.
About Integrin αvβ3
- Integrin αvβ3 is a protein complex that has key role in cell adhesion, signal transduction across the cell membrane, and cell survival.
- This protein recognizes a specific sequence of amino acids known as arginine-glycine-aspartic acid (RGD).
Objective of the Study
- While the function of integrin αvβ3 is widely studied in various organisms, its expression in certain parts of a horse’s body, specifically the lungs and jejunum, isn’t well understood.
- The objective of this research was hence to investigate the expression of this specific protein in these areas using light and electron immunocytochemistry.
Findings
- Immunohistochemistry, which is a technique used to locate proteins in cells of a tissue section, showed the presence of integrin αvβ3 on a variety of locations including the epithelium, immune cells in Peyer’s patches (areas of the small intestine where immune response is initiated), smooth muscle, and the endothelium of equine jejunum (part of the small intestine).
- Within equine lungs, integrin αvβ3 was identified on the endothelium of blood vessels, the alveolar septa (thin walls between two alveoli), bronchial lymph nodes, and the cartilages. However, the expression of the protein was limited on the epithelium of bronchioles (small branches of bronchi).
Conclusion
- The study concludes with it being the first of its kind to identify the expression of integrin αvβ3 in the lungs and jejunum of horses. This opens up new avenues for further research in the functions of this protein in these specific areas.
Cite This Article
APA
Le NPK, Gerdts V, Singh B.
(2020).
Integrin alpha-v/beta3 expression in equine lungs and jejunum.
Can J Vet Res, 84(4), 245-251.
Publication
Researcher Affiliations
- Western College of Veterinary Medicine (Le, Gerdts, Singh) and Vaccine and Infectious Disease Organization - International Vaccine Centre (Gerdts), University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Faculty of Animal Science and Veterinary Medicine, Nong Lam University, Ho Chi Minh City, Vietnam (Le); Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta T2N 4Z6 (Singh).
- Western College of Veterinary Medicine (Le, Gerdts, Singh) and Vaccine and Infectious Disease Organization - International Vaccine Centre (Gerdts), University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Faculty of Animal Science and Veterinary Medicine, Nong Lam University, Ho Chi Minh City, Vietnam (Le); Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta T2N 4Z6 (Singh).
- Western College of Veterinary Medicine (Le, Gerdts, Singh) and Vaccine and Infectious Disease Organization - International Vaccine Centre (Gerdts), University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4; Faculty of Animal Science and Veterinary Medicine, Nong Lam University, Ho Chi Minh City, Vietnam (Le); Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta T2N 4Z6 (Singh).
MeSH Terms
- Animals
- Gene Expression Regulation / physiology
- Horses / metabolism
- Integrin alphaVbeta3 / genetics
- Integrin alphaVbeta3 / metabolism
- Jejunum / metabolism
- Lung / metabolism
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