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Intrinsic innervation of the horse ileum.
Abstract: This paper describes the morphology and distribution of the enteric nervous system (ENS) cells and fibres immunoreactive for choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), substance P (SP), calcitonin gene-related peptide (CGRP), NF200kDa (NF200), and S100 protein. The percentages of subclasses of enteric neurons in the total neuronal population were investigated by the use of anti-PGP 9.5 or anti-NSE antibodies. ChAT-IR myenteric plexus (MP) and submucosal plexus (SMP) neurons were 66+/-7% and 74+/-15%, respectively, whereas those cells expressing nNOS-IR were 38+/-7% and 5+/-1%, respectively. MP and SMP neurons expressing both phenotypes were also present. SP-IR was expressed by 14+/-13% of MP and 66+/-8% of SMP neurons whereas CGRP-IR was observed only in the SMP (43+/-6%). NF200-IR was expressed by 61+/-15% and 91+/-10% of the MP and SMP neurons, respectively. The majority of the CGRP-IR SMP neurons expressed also SP-IR. Almost all SP-IR neurons in both the plexuses were cholinergic. The present study quantifies the main neuronal subpopulations of the ENS of the horse ileum; these data might be utilized to understand the neuronal modifications which occur in several gastrointestinal tract disorders.
Publication Date: 2009-04-19 PubMed ID: 19380154DOI: 10.1016/j.rvsc.2009.03.011Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article explores the structure and distribution of specific cells and fibers in the enteric nervous system (ENS) of the horse’s ileum. The researchers measure the quantity of subsets of enteric neurons in relation to the total neuronal population and talk about their potential use in understanding changes in the nervous system in various gastrointestinal tract disorders.
Structure and Distribution of the Enteric Nervous System (ENS)
The paper focuses on the presentation of distinct cells and fibers in the ileum’s ENS, which are crucial for its functioning. The cells and fibers’ morphology and distribution were critically examined. The research paid attention to cells and fibres that reacted to the following:
- Choline acetyltransferase (ChAT)
- Neuronal Nitric Oxide Synthase (nNOS)
- Substance P (SP)
- Calcitonin gene-related peptide (CGRP)
- NF200kDa (NF200)
- S100 protein
Quantifying Subclasses of Enteric Neurons
The research utilized anti-PGP 9.5 or anti-NSE antibodies to investigate the percentages of subclasses of enteric neurons in the total neuronal population. These subpopulations serve different functions within the ENS. The study provides detailed percentages for different types of neurons expressing markers like ChAT, nNOS, SP, CGRP, NF200-IR in two significant sections of the ENS: Myenteric Plexus (MP) and Submucosal Plexus (SMP).
Phenotypes and Co-Expression
MP and SMP neurons demonstrating both ChAT and nNOS phenotypes were found. The study also noted the percentage of neurons expressing SP-IR and CGRP-IR, with differing distributions in the MP and SMP. The majority of the CGRP-IR SMP neurons also expressed SP-IR, and nearly all of the SP-IR neurons in both plexuses were found to be cholinergic.
Implications for Gastrointestinal Tract Disorders
The study concludes by highlighting the potential applications of these quantitative data in understanding the neuronal modifications that occur in a variety of gastrointestinal tract disorders. This could potentially lead to enhanced approaches to the treatment and management of such conditions.
Cite This Article
APA
Chiocchetti R, Bombardi C, Mongardi-Fantaguzzi C, Venturelli E, Russo D, Spadari A, Montoneri C, Romagnoli N, Grandis A.
(2009).
Intrinsic innervation of the horse ileum.
Res Vet Sci, 87(2), 177-185.
https://doi.org/10.1016/j.rvsc.2009.03.011 Publication
Researcher Affiliations
- Department of Veterinary Morphophysiology and Animal Productions, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy. roberto.chiocchetti@unibo.it
MeSH Terms
- Animals
- Antibody Specificity
- Enteric Nervous System / cytology
- Enteric Nervous System / physiology
- Female
- Horses
- Ileum / innervation
- Immunohistochemistry
- Intestinal Mucosa / innervation
- Microscopy, Fluorescence
- Myenteric Plexus / cytology
- Myenteric Plexus / physiology
- Neurons / cytology
- Neurons / enzymology
- Neurons / physiology
- Nitric Oxide Synthase Type I / analysis
Citations
This article has been cited 6 times.- Diana A, Freccero F, Giancola F, Linta N, Pietra M, Luca V, Salamanca G, Cipone M, Chiocchetti R. Ex vivo ultrasonographic and histological morphometry of small intestinal wall layers in horses. Vet Radiol Ultrasound 2022 May;63(3):353-363.
- Brehmer A. Classification of human enteric neurons. Histochem Cell Biol 2021 Aug;156(2):95-108.
- Bombardi C, Rambaldi AM, Galiazzo G, Giancola F, Graïc JM, Salamanca G, Cozzi B, Chiocchetti R. Nitrergic and Substance P Immunoreactive Neurons in the Enteric Nervous System of the Bottlenose Dolphin (Tursiops truncatus) Intestine. Animals (Basel) 2021 Apr 8;11(4).
- Toschi A, Galiazzo G, Piva A, Tagliavia C, Mazzuoli-Weber G, Chiocchetti R, Grilli E. Cannabinoid and Cannabinoid-Related Receptors in the Myenteric Plexus of the Porcine Ileum. Animals (Basel) 2021 Jan 21;11(2).
- Chiocchetti R, Giancola F, Mazzoni M, Sorteni C, Romagnoli N, Pietra M. Excitatory and inhibitory enteric innervation of horse lower esophageal sphincter. Histochem Cell Biol 2015 Jun;143(6):625-35.
- Forrest SL, Osborne PB, Keast JR. Characterization of axons expressing the artemin receptor in the female rat urinary bladder: a comparison with other major neuronal populations. J Comp Neurol 2014 Dec 1;522(17):3900-27.
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