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Home / Equine Research Bank / J Vet Sci / Isolation and characterization of equine amniotic membrane-d...
Journal of veterinary science2013; 14(2); 151-159; doi: 10.4142/jvs.2013.14.2.151

Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells.

Abstract: Recent studies have shown that mesenchymal stem cells (MSCs) are able to differentiate into multi-lineage cells such as adipocytes, chondroblasts, and osteoblasts. Amniotic membrane from whole placenta is a good source of stem cells in humans. This membrane can potentially be used for wound healing and corneal surface reconstruction. Moreover, it can be easily obtained after delivery and is usually discarded as classified waste. In the present study, we successfully isolated and characterized equine amniotic membrane-derived mesenchymal stem cells (eAM-MSCs) that were cultured and maintained in low glucose Dulbecco's modified Eagle's medium. The proliferation of eAM-MSCs was measured based on the cumulative population doubling level (CPDL). Immunophenotyping of eAM-MSCs by flow cytometry showed that the major population was of mesenchymal origin. To confirm differentiation potential, a multi-lineage differentiation assay was conducted. We found that under appropriate conditions, eAM-MSCs are capable of multi-lineage differentiation. Our results indicated that eAM-MSCs may be a good source of stem cells, making them potentially useful for veterinary regenerative medicine and cell-based therapy.
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Publication Date: 2013-02-05 PubMed ID: 23388430PubMed Central: PMC3694186DOI: 10.4142/jvs.2013.14.2.151Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the potential use of mesenchymal stem cells (MSCs) from horse amniotic membrane for use in regenerative veterinary medicine. The study successfully extracted and characterized these equine amniotic membrane-derived mesenchymal stem cells (eAM-MSCs), finding them capable of differentiating into multiple cell lines.

Study Procedure and Results

  • The scientists obtained amniotic membranes from equine placenta, typically discarded as waste after delivery. This provided a readily available and non-invasive source of cellular material.
  • The extracted eAM-MSCs were cultured and maintained in low glucose Dulbecco’s modified Eagle’s medium, a common nutritive bath used in cell culture.
  • The growth and proliferation of eAM-MSCs were measured using the Cumulative Population Doubling Level (CPDL), a technique that keeps track of the total number of times the cells have doubled since their initial population stage.
  • Immunophenotyping, a process to study the proteins on the surface of cells, helped characterize the eAM-MSCs. The majority population of cells were found to be of mesenchymal origin, which indicates their ability to differentiate into various cell types.
  • The potential for eAM-MSCs to differentiate into other cells was confirmed with a multi-lineage differentiation assay. This process exposed cells to particular conditions to spur transformation into other cell forms, such as fat cells or bone cells.

Significance of the Research

  • The study confirms that eAM-MSCs are capable of multi-lineage differentiation, suggesting their possible use in regenerative veterinary medicine. With their remarkable ability to transform into various cell types, these stem cells could potentially contribute to healing and regenerating damaged tissues in animals.
  • The research also highlights the role of eAM-MSCs as a potential resource for cell-based therapies – disease treatments that use cellular material to resolve medical conditions.

Future Implications

  • Applying the results of this research could lead to advanced veterinary procedures. With further investigation, eAM-MSCs could be a valuable tool in treating diseases and injuries in equine medicine.
  • This research could also inspire similar investigations in other animal species, potentially expanding therapeutic intra-species or cross-species applications.

Cite This Article

APA
Seo MS, Park SB, Kim HS, Kang JG, Chae JS, Kang KS. (2013). Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells. J Vet Sci, 14(2), 151-159. https://doi.org/10.4142/jvs.2013.14.2.151

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 14
Issue: 2
Pages: 151-159

Researcher Affiliations

Seo, Min-Soo
  • Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Park, Sang-Bum
    Kim, Hyung-Sik
      Kang, Jun-gu
        Chae, Joon-Seok
          Kang, Kyung-Sun

            MeSH Terms

            • Adipogenesis
            • Amnion / cytology
            • Amnion / physiology
            • Animals
            • Cell Differentiation
            • Cell Lineage
            • Cell Proliferation
            • Chondrogenesis
            • Female
            • Flow Cytometry / veterinary
            • Horses
            • Immunophenotyping / veterinary
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / physiology
            • Osteogenesis

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            Citations

            This article has been cited 9 times.
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