Isolation and Evaluation of Probiotic Potential of Lactic Acid Strains From Healthy Equines for Potential Use in Salmonella Infection.
Abstract: The objective of the present study was to evaluate the probiotic properties, security and antibacterial ability in vivo of isolated strains from healthy equine. In the present study, two Pediococcus acidilactici (P1 and P2) and two Lactobacillus equi (L1 and L2) were isolated. All isolates were died when exposed to pH 2.0 for 3 hours but survived at pH 3.0 and pH 4.0 with differential survival rate, and there is a higher survival rate at pH 4.0. Similarly, the isolates showed different tolerance to bile. The viable bacteria count was sustained at high levels in a tolerance test with artificial gastrointestinal fluid. The isolates survived and grew at temperatures between 37 and 55°C but died at 65°C. Four strains exhibited inhibitory activity against pathogens, including Salmonella typhimurium (CVCC542), Escherichia coli (C83902), Staphylococcus aureus (BNCC186335), and Pasteurella multocida (clinical isolate). These isolates exhibited differential antibiotic susceptibility. In safety trials, all isolates were γ-hemolytic, and the oral toxicity of strains P1 (gavaged with 1 × 109 CFU/day) and L1 (gavaged with 1 × 109 CFU/day) were analyzed in mice. There were no effects on the overall health status of mice. There were no prominent differences in the incidence of bacteria translocation to blood, liver, and spleen. Mice gavaged with Pediococcus acidilactici P1 (1 × 108 CFU/day) or Lactobacillus equi L1 (1 × 108 CFU/day) as prevention showed lower rates of diarrhea and mortality after being challenged with Salmonella typhimurium (4 × 106 CFU signal dose, 0.1 mL by intragastric gavage). The results indicate that the isolated strains could act as potential probiotics, providing a new way to reduce salmonella infection, which merit future application studies.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication Date: 2020-11-01 PubMed ID: 33349402DOI: 10.1016/j.jevs.2020.103312Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research aims to examine the probiotic properties of isolated strains from healthy horses for potential use against Salmonella infections, with promising results indicating these strains could present a new method for reducing such infections.
Research Methodology and Results
- The study investigated two strains each of Pediococcus acidilactici (P1 and P2) and Lactobacillus equi (L1 and L2), all derived from healthy horses. These strains showed varying survival rates under different acidities, with all dying at a pH of 2.0 but surviving under milder acidities (pH 3.0 and 4.0).
- All isolates also showed varying tolerance levels to bile and remained viable in high numbers during a tolerance test with artificial gastrointestinal fluid.
- They were able to survive and grow within temperature ranges of 37 to 55°C but perished at 65°C.
- Interestingly, these isolated strains demonstrated inhibitory activity against pathogenic organisms such as Salmonella typhimurium, Escherichia coli, Staphylococcus aureus, and Pasteurella multocida.
- These isolates also exhibited different levels of antibiotic susceptibility; however, the study didn’t delve deeper into what types of antibiotics or the exact degree of susceptibility.
Safety Trials and Potential Probiotic Action
- In safety trials, all of the mentioned isolates were γ-hemolytic (they didn’t break down red blood cells), and no negative effects were noticed in mice that received a daily dose of strains P1 or L1.
- Further, there were no significant differences in bacterial translocation to the blood, liver, or spleen of the tested mice, suggesting that the tested strains didn’t cause infection or inflammation in these organs.
- Importantly, mice that were given these strains as preventive treatment showcased lower rates of diarrhea and mortality when later challenged with Salmonella typhimurium.
- This finding suggests these isolates might have significant potential as probiotics, which are beneficial bacteria that promote gut health and can protect against certain diseases.
To conclude, this study isolates certain lactic acid strains from healthy horses that could prove beneficial in preventing or reducing Salmonella infections. Further studies are, however, required to validate these results and explore specific application methods.
Cite This Article
APA
Pei L, Yang H, Qin S, Yan Z, Zhang H, Lan Y, Li A, Iqbal M, Shen Y.
(2020).
Isolation and Evaluation of Probiotic Potential of Lactic Acid Strains From Healthy Equines for Potential Use in Salmonella Infection.
J Equine Vet Sci, 96, 103312.
https://doi.org/10.1016/j.jevs.2020.103312 Publication
Researcher Affiliations
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
- Wuhan Business University, Wuhan, Hubei, China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. Electronic address: yshen@mail.hzau.edu.cn.
MeSH Terms
- Animals
- Health Status
- Horse Diseases
- Horses
- Lactic Acid
- Lactobacillus
- Mice
- Probiotics / therapeutic use
- Salmonella Infections
Citations
This article has been cited 5 times.- Shaker RAE, Hashem RA, Hassan M, Ibrahim AM, Ragab YM, Khattab RA. Probiotic, antimicrobial and anticancer properties of Lysinibacillus macroides, Kurthia huakuii, and Enterococcus faecium isolated from freshwater snail gut microbiota. BMC Biotechnol 2025 Sep 3;25(1):98.
- Xu M, Hu M, Han J, Wang L, He Y, Kulyar MF, Zhang X, Lu Y, Mu S, Su H, Cao J, Li J. The Therapeutic Effects of Lactic Acid Bacteria Isolated from Spotted Hyena on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice. Nutrients 2024 Oct 29;16(21).
- Evangelista AG, Matté EHC, Corrêa JAF, Gonçalves FDR, Dos Santos JVG, Biauki GC, Milek MM, Costa LB, Luciano FB. Bioprotective potential of lactic acid bacteria for Salmonella biocontrol in vitro. Vet Res Commun 2023 Sep;47(3):1357-1368.
- Li XB, Huang XX, Li Q, Li XY, Li JH, Li C, He LJ, Jing HX, Yang KL. Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach. BMC Vet Res 2022 Nov 17;18(1):407.
- Pei L, Liu J, Huang Z, Iqbal M, Shen Y. Effects of Lactic Acid Bacteria Isolated from Equine on Salmonella-Infected Gut Mouse Model. Probiotics Antimicrob Proteins 2023 Jun;15(3):469-478.
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