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Veterinary research communications2009; 33(8); 811-821; doi: 10.1007/s11259-009-9229-0

Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor.

Abstract: Mesenchymal stem cells (MSC) are increasingly used as therapeutical aid for the orthopaedic injuries in the horse. MSC populate different tissues but the most commonly used for clinical purposes are isolated from bone marrow or adipose tissue. The first objective of this study was to investigate if the donor animal, the tissue of origin and the technique of isolation could influence the number of MSC available for transplantation after a short-term expansion. The second aim was to devise a culture system capable of increasing MSC lifespan and we tested the effect of basic fibroblast growth factor (bFGF). Results indicate that MSC can be efficiently isolated from both sources and supplementation of bFGF enhances proliferation rate maintaining differentiation potential. In addition, this study shows that collection, expansion and storage of frozen MSC can be performed for later therapeutic use.
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Publication Date: 2009-05-28 PubMed ID: 19472068DOI: 10.1007/s11259-009-9229-0Google Scholar: Lookup
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  • Journal Article
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the effect of donor characteristics, source tissue, and isolation technique on the availability of mesenchymal stem cells (MSC) for transplantation. The study also investigates the impact of basic fibroblast growth factor (bFGF) on MSC lifespan and differentiation. The findings suggest that MSC can be efficiently isolated from both bone marrow and adipose tissue, and that the addition of bFGF can boost cell proliferation while maintaining their potential for differentiation.

Goal and Objectives of the Research

  • The study aimed to understand the influence of particular variables on the availability of MSC for transplantation. These variables included the characteristics of the donor animal, the tissue from which the cells are extracted (bone marrow or adipose tissue), and the technique used for cell isolation.
  • A secondary objective was to develop a culture system that could enhance the lifespan of MSC. The researchers tested this by supplementing the system with basic fibroblast growth factor (bFGF), examining its effect on cell proliferation and differentiation.

Findings and Implications

  • The results show that efficient isolation of MSC can be achieved from both bone marrow and adipose tissue. This is significant as it suggests multiple viable sources for these stem cells, which are increasingly being used as a therapeutic aid for orthopaedic injuries in horses.
  • Supplementation with bFGF was shown to increase the rate of MSC proliferation, while still maintaining their differentiation potential. This could have implications for extending the usable lifespan of MSC, potentially enhancing their therapeutic potential.
  • The researchers also demonstrated that it is feasible to collect, expand, and store MSC for future therapeutic use. This could offer benefits in terms of ease and convenience for both the donor animals and those receiving the transplants.

Cite This Article

APA
Colleoni S, Bottani E, Tessaro I, Mari G, Merlo B, Romagnoli N, Spadari A, Galli C, Lazzari G. (2009). Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor. Vet Res Commun, 33(8), 811-821. https://doi.org/10.1007/s11259-009-9229-0

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 33
Issue: 8
Pages: 811-821

Researcher Affiliations

Colleoni, Silvia
  • Laboratorio di Tecnologie della Riproduzione, Avantea srl, Via Porcellasco 7/f, 26100 Cremona, Italy. silviacolleoni@avantea.it
Bottani, Emanuela
    Tessaro, Irene
      Mari, Gaetano
        Merlo, Barbara
          Romagnoli, Noemi
            Spadari, Alessandro
              Galli, Cesare
                Lazzari, Giovanna

                  MeSH Terms

                  • Adipose Tissue / cytology
                  • Animals
                  • Bone Marrow Cells / physiology
                  • Cell Differentiation / physiology
                  • Cell Proliferation
                  • Cells, Cultured
                  • Fibroblast Growth Factor 2 / physiology
                  • Horses
                  • Mesenchymal Stem Cell Transplantation / veterinary
                  • Mesenchymal Stem Cells / physiology

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