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Home / Equine Research Bank / Vaccine / Local and systemic isotype-specific antibody responses to eq...
Vaccine1998; 16(13); 1306-1313; doi: 10.1016/s0264-410x(98)00009-7

Local and systemic isotype-specific antibody responses to equine influenza virus infection versus conventional vaccination.

Abstract: Inactivated alum-adjuvanted conventional equine influenza virus vaccines are of poor efficacy and offer limited short-term protection against infection. In sharp contrast, natural infection with equine influenza virus confers long-term protective immunity. In order to identify the protective immune responses to equine influenza virus, the influenza virus-specific IgA, IgGa, IgGb, IgGc and IgG(T) antibody responses in nasal secretions and serum induced by natural infection and a commercial vaccine were studied by ELISA. Two groups of four influenza-naive ponies were established. In the natural infection group, ponies received 10(8.5) EID50 of A/equine/Ky/1/81 by intranasal instillation, were allowed to recover, and then were rechallenged 100 days later. All four ponies exhibited clinical signs of influenza virus infection and viral shedding following primary infection, but were completely protected from challenge infection. Antibody responses to primary infection were characterized by nasal IgA and serum IgGa and IgGb responses. Ponies in the conventional vaccine group received a commercially available vaccine by intramuscular injection followed by a booster injection 3 weeks later. Challenge infection 100 days after vaccination resulted in clinical signs of infection and viral shedding. Antibody responses to vaccination were restricted to serum IgG(T) responses only. These results demonstrate that the protective immunity generated by natural equine influenza virus infection is associated with a mucosal IgA immune response and humoral IgGa and IgGb sub-isotype responses, and that this pattern of response is not generated by conventional vaccines.
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Publication Date: 1998-07-31 PubMed ID: 9682395DOI: 10.1016/s0264-410x(98)00009-7Google Scholar: Lookup
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  • Clinical Trial
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Equine influenza vaccines don’t offer long-term protection against the virus, unlike the immunity developed from a naturally occurring infection. This research scrutinizes the differences in immune responses between horses recovering from an infection and those receiving typical vaccines. It suggests that key factors of post-infection protection may be the antibodies IgA, IgGa and IgGb, which are notably absent from the immune response to conventional vaccines.

Methodology

  • Researchers set up two groups of four ponies each—naive to the influenza infection—to evaluate their bodily responses. The first group was naturally infected via an intranasal instillation of A/equine/Ky/1/81 viral strain, then the horses were allowed to recover before facing a secondary challenge 100 days later.
  • The second group, however, was instead administered with a commercial vaccine via an injection in the muscles, followed by a booster shot three weeks afterwards. They were then exposed to the virus 100 days post-vaccination for comparison.

Findings

  • During the primary infection, all four ponies exhibited typical clinical signs of an influenza virus infection and viral shedding. However, they were wholly protected from the secondary exposure to the virus, showing no illness.
  • The ponies naturally infected had immune responses defined by nasal IgA and serum IgGa and IgGb antibodies, showing a distinctive pattern of response that was absent in the vaccinated group.
  • Conversely, the vaccinated ponies still displayed signs of an infection upon exposure to the virus after the vaccination, signifying that the immunization did not provide comprehensive protection.
  • The vaccine elicited only serum IgG(T) antibody responses, and did not stimulate the mucosal IgA, IgGa, and IgGb antibodies induced by natural infection, which seem crucial for protection.

Conclusion

  • This research concludes that the protective immunity generated by natural equine influenza virus infection is associated with a distinct mucosal IgA immune response and humoral IgGa and IgGb sub-isotype responses—a pattern that is not produced by conventional vaccines. This suggests that future equine influenza vaccines need to account for this to improve their efficacy and produce long-term immunity.

Cite This Article

APA
Nelson KM, Schram BR, McGregor MW, Sheoran AS, Olsen CW, Lunn DP. (1998). Local and systemic isotype-specific antibody responses to equine influenza virus infection versus conventional vaccination. Vaccine, 16(13), 1306-1313. https://doi.org/10.1016/s0264-410x(98)00009-7

Publication

Vaccine
ISSN: 0264-410X
NlmUniqueID: 8406899
Country: Netherlands
Language: English
Volume: 16
Issue: 13
Pages: 1306-1313

Researcher Affiliations

Nelson, K M
  • Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
Schram, B R
    McGregor, M W
      Sheoran, A S
        Olsen, C W
          Lunn, D P

            MeSH Terms

            • Animals
            • Antibodies, Viral / biosynthesis
            • Horse Diseases / immunology
            • Horse Diseases / prevention & control
            • Horses
            • Immunity, Mucosal
            • Immunoglobulin A / biosynthesis
            • Immunoglobulin A, Secretory / biosynthesis
            • Immunoglobulin G / biosynthesis
            • Influenza A virus / immunology
            • Influenza Vaccines / immunology
            • Nasal Mucosa / immunology
            • Orthomyxoviridae Infections / immunology
            • Orthomyxoviridae Infections / prevention & control
            • Orthomyxoviridae Infections / veterinary
            • Recurrence
            • Vaccination / veterinary
            • Virus Shedding / immunology

            Citations

            This article has been cited 20 times.
            1. Carnet F, Perrin-Cocon L, Paillot R, Lotteau V, Pronost S, Vidalain PO. An inventory of adjuvants used for vaccination in horses: the past, the present and the future. Vet Res 2023 Mar 2;54(1):18.
              doi: 10.1186/s13567-023-01151-3pubmed: 36864517google scholar: lookup
            2. Anna M, Łukasz M, Adam O, Chełmońska-Soyta A. Effectiveness of immunization with multi-component bacterial immunomodulator in foals at 35th day of life. Sci Rep 2022 Sep 22;12(1):15795.
              doi: 10.1038/s41598-022-17532-1pubmed: 36138050google scholar: lookup
            3. El-Hage C, Hartley C, Savage C, Watson J, Gilkerson J, Paillot R. Assessment of Humoral and Long-Term Cell-Mediated Immune Responses to Recombinant Canarypox-Vectored Equine Influenza Virus Vaccination in Horses Using Conventional and Accelerated Regimens Respectively. Vaccines (Basel) 2022 May 26;10(6).
              doi: 10.3390/vaccines10060855pubmed: 35746463google scholar: lookup
            4. Oladunni FS, Oseni SO, Martinez-Sobrido L, Chambers TM. Equine Influenza Virus and Vaccines. Viruses 2021 Aug 20;13(8).
              doi: 10.3390/v13081657pubmed: 34452521google scholar: lookup
            5. Zarski LM, Vaala WE, Barnett DC, Bain FT, Soboll Hussey G. A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1. Front Vet Sci 2021;8:674850.
              doi: 10.3389/fvets.2021.674850pubmed: 34179166google scholar: lookup
            6. Thorsteinsdóttir L, Jónsdóttir S, Stefánsdóttir SB, Andrésdóttir V, Wagner B, Marti E, Torsteinsdóttir S, Svansson V. The effect of maternal immunity on the equine gammaherpesvirus type 2 and 5 viral load and antibody response. PLoS One 2019;14(6):e0218576.
              doi: 10.1371/journal.pone.0218576pubmed: 31226153google scholar: lookup
            7. Rocha JN, Dangott LJ, Mwangi W, Alaniz RC, Bordin AI, Cywes-Bentley C, Lawhon SD, Pillai SD, Bray JM, Pier GB, Cohen ND. PNAG-specific equine IgG(1) mediates significantly greater opsonization and killing of Prescottella equi (formerly Rhodococcus equi) than does IgG(4/7). Vaccine 2019 Feb 21;37(9):1142-1150.
              doi: 10.1016/j.vaccine.2019.01.028pubmed: 30691984google scholar: lookup
            8. Singh RK, Dhama K, Karthik K, Khandia R, Munjal A, Khurana SK, Chakraborty S, Malik YS, Virmani N, Singh R, Tripathi BN, Munir M, van der Kolk JH. A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies. Front Microbiol 2018;9:1941.
              doi: 10.3389/fmicb.2018.01941pubmed: 30237788google scholar: lookup
            9. Sandbulte MR, Spickler AR, Zaabel PK, Roth JA. Optimal Use of Vaccines for Control of Influenza A Virus in Swine. Vaccines (Basel) 2015 Jan 30;3(1):22-73.
              doi: 10.3390/vaccines3010022pubmed: 26344946google scholar: lookup
            10. Ngunjiri JM, Ali A, Boyaka P, Marcus PI, Lee CW. In vivo assessment of NS1-truncated influenza virus with a novel SLSYSINWRH motif as a self-adjuvanting live attenuated vaccine. PLoS One 2015;10(3):e0118934.
              doi: 10.1371/journal.pone.0118934pubmed: 25790187google scholar: lookup
            11. Bordin AI, Pillai SD, Brake C, Bagley KB, Bourquin JR, Coleman M, Oliveira FN, Mwangi W, McMurray DN, Love CC, Felippe MJ, Cohen ND. Immunogenicity of an electron beam inactivated Rhodococcus equi vaccine in neonatal foals. PLoS One 2014;9(8):e105367.
              doi: 10.1371/journal.pone.0105367pubmed: 25153708google scholar: lookup
            12. Ault A, Zajac AM, Kong WP, Gorres JP, Royals M, Wei CJ, Bao S, Yang ZY, Reedy SE, Sturgill TL, Page AE, Donofrio-Newman J, Adams AA, Balasuriya UB, Horohov DW, Chambers TM, Nabel GJ, Rao SS. Immunogenicity and clinical protection against equine influenza by DNA vaccination of ponies. Vaccine 2012 Jun 6;30(26):3965-74.
              doi: 10.1016/j.vaccine.2012.03.026pubmed: 22449425google scholar: lookup
            13. Marcus PI, Ngunjiri JM, Sekellick MJ, Wang L, Lee CW. In vitro analysis of virus particle subpopulations in candidate live-attenuated influenza vaccines distinguishes effective from ineffective vaccines. J Virol 2010 Nov;84(21):10974-81.
              doi: 10.1128/JVI.00502-10pubmed: 20739541google scholar: lookup
            14. Wang L, Suarez DL, Pantin-Jackwood M, Mibayashi M, García-Sastre A, Saif YM, Lee CW. Characterization of influenza virus variants with different sizes of the non-structural (NS) genes and their potential as a live influenza vaccine in poultry. Vaccine 2008 Jul 4;26(29-30):3580-6.
              doi: 10.1016/j.vaccine.2008.05.001pubmed: 18539366google scholar: lookup
            15. Lewis MJ, Wagner B, Woof JM. The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies. Mol Immunol 2008 Feb;45(3):818-27.
              doi: 10.1016/j.molimm.2007.06.158pubmed: 17669496google scholar: lookup
            16. Quinlivan M, Zamarin D, García-Sastre A, Cullinane A, Chambers T, Palese P. Attenuation of equine influenza viruses through truncations of the NS1 protein. J Virol 2005 Jul;79(13):8431-9.
              doi: 10.1128/JVI.79.13.8431-8439.2005pubmed: 15956587google scholar: lookup
            17. Morton HC, Pleass RJ, Storset AK, Brandtzaeg P, Woof JM. Cloning and characterization of equine CD89 and identification of the CD89 gene in chimpanzees and rhesus macaques. Immunology 2005 May;115(1):74-84.
              doi: 10.1111/j.1365-2567.2005.02129.xpubmed: 15819699google scholar: lookup
            18. Hooper-McGrevy KE, Wilkie BN, Prescott JF. Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins. Clin Diagn Lab Immunol 2003 May;10(3):345-51.
              doi: 10.1128/cdli.10.3.345-351.2003pubmed: 12738629google scholar: lookup
            19. Khatibzadeh SM, Gold CB, Keggan AE, Perkins GA, Glaser AL, Dubovi EJ, Wagner B. West Nile virus-specific immunoglobulin isotype responses in vaccinated and infected horses. Am J Vet Res 2015 Jan;76(1):92-100.
              doi: 10.2460/ajvr.76.1.92pubmed: 25535666google scholar: lookup
            20. Keggan A, Freer H, Rollins A, Wagner B. Production of seven monoclonal equine immunoglobulins isotyped by multiplex analysis. Vet Immunol Immunopathol 2013 Jun 15;153(3-4):187-93.
              doi: 10.1016/j.vetimm.2013.02.010pubmed: 23541920google scholar: lookup
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