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Pathogens (Basel, Switzerland)2021; 10(9); 1069; doi: 10.3390/pathogens10091069

Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea.

Abstract: The horse industry has grown rapidly as a leisure industry in the Republic of Korea (ROK) in parallel with an increased demand for equestrian activities. As a result, there has been an increase in horse breeding and equestrian population and potential exposure to ticks and their associated pathogens. To provide a better understanding of the potential disease risks of veterinary and medical importance, a study was conducted to determine the geographical distribution and diversity of ticks collected from horses and vegetation associated with horse racetracks/ranches throughout the ROK. This included a survey of five associated common pathogens, , , spp., , and . A total 9220 ticks were collected from horses and associated pastures. Ticks were identified to species, stage of development, and sex. Two species of ticks, (99.9%) and (0.1%) were identified. Two of the target pathogens, and spp., were detected in 5/1409 tick pools (0.35%) and 4/1409 pools (0.28%) of , respectively, both of which are zoonotic pathogens of medical importance. The results of 16S rRNA phylogenetic analysis of showed a close relationship to strains distributed in China, USA, Germany, Italy, Turkey, and Poland. spp. showed a close relationship, based on 16S rRNA gene, to the strains reported from the USA ( and ) and Japan ( and ). These results provide information about the potential risks of veterinary and medical importance and the development of mitigation strategies for disease prevention.
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Publication Date: 2021-08-24 PubMed ID: 34578102PubMed Central: PMC8472514DOI: 10.3390/pathogens10091069Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study conducted on identifying ticks and their pathogens collected from horses in the Republic of Korea, given the recent growth in the horse industry. This study helps in understanding the potential risks these ticks pose to veterinary and medical fields.

Objective and Scope of the Research

  • The research aimed to identify and analyze the different species of ticks and the common pathogens they carry that may have been collected from horses and associated pastures in the Republic of Korea.
  • The study came about due to the rapid growth of the horse industry in Korea, which has resulted in an increase in horse breeding and equestrian activities, leading to potential exposure to specific ticks and their associated pathogens.
  • In order to better understand the potential health risks these ticks can bring, the study focused on the geographical distribution and variety of ticks in these regions.

Methodology of the Study

  • A total of 9220 ticks were identified regarding their species, stage of development, and sex. They were collected from horses and associated vegetation near horse racetracks and ranches.
  • The study included a survey of five common pathogens detected in ticks which may pose risks to both animal and human health.
  • Ticks found were majorly of two species – 99.9% belonging to one species and the remaining 0.1% to another species.

Findings from the Research

  • Two pathogens of medical importance were detected with the help of pool testing in ticks of one particular species.
  • These pathogens are also known to be zoonotic, indicating that they can be transmitted from animals to humans.
  • To understand the global perspective, the researchers performed a 16S rRNA phylogenetic analysis on the pathogens detected. They found a close relationship between the pathogens distributed in several countries like China, USA, Germany, Italy, Turkey, Poland, and Japan.

Impact of Study

  • The outcomes of this study impart vital information regarding potential risks that ticks and their associated pathogens may present to health in veterinary and medical fields.
  • Based on these findings, appropriate strategies for disease prevention can be developed and implemented.

Cite This Article

APA
Seo HJ, Truong AT, Kim KH, Lim JY, Min S, Kim HC, Yoo MS, Yoon SS, Klein TA, Cho YS. (2021). Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea. Pathogens, 10(9), 1069. https://doi.org/10.3390/pathogens10091069

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 10
Issue: 9
PII: 1069

Researcher Affiliations

Seo, Hyun-Ji
  • Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Truong, A-Tai
  • Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
  • Faculty of Biotechnology, Thai Nguyen University of Sciences, Thai Nguyen 2500000, Vietnam.
Kim, Keun-Ho
  • Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Lim, Ji-Yeon
  • Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Min, Subin
  • Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Kim, Heung-Chul
  • Force Health Protection and Prevention Medicine, Medical Department Activity-Korea, 65th Medical Brigrade, Unit 15281, APO AP 96271-5281, USA.
Yoo, Mi-Sun
  • Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Yoon, Soon-Seek
  • Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Klein, Terry A
  • Force Health Protection and Prevention Medicine, Medical Department Activity-Korea, 65th Medical Brigrade, Unit 15281, APO AP 96271-5281, USA.
Cho, Yun Sang
  • Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.

Grant Funding

  • P-1543084-2019-21-0101 / Animal and Plant Quarantine Agency
  • ProMIS ID P0025_17_ME / Armed Forces Health Surveillance Division, Global Emerging Infections Surveillance (GEIS) Section

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

This article includes 52 references
  1. Horse Industry Status Report (HISR), Ministry of Agriculture, Food and Rural Affairs. 2019. [(accessed on 10 February 2021)]. Available online: www.horsepia.com/industry/stat/horseAllStat.do.
  2. Kim J-Y. The Present Condition and Prospect of Korean Horse Industry. Int. J. Multimed. Ubiquitous Eng. 2015;10:119–124.
    doi: 10.14257/ijmue.2015.10.12.13google scholar: lookup
  3. Laus F, Veronesi F, Passamonti F, Paggi E, Cerquetella M, Hyatt D, Tesei B, Fioretti DP. Prevalence of tick borne pathogens in horses from Italy.. J Vet Med Sci 2013;75(6):715-20.
    doi: 10.1292/jvms.12-0449pubmed: 23328633google scholar: lookup
  4. Ueti MW, Palmer GH, Scoles GA, Kappmeyer LS, Knowles DP. Persistently infected horses are reservoirs for intrastadial tick-borne transmission of the apicomplexan parasite Babesia equi.. Infect Immun 2008 Aug;76(8):3525-9.
    doi: 10.1128/IAI.00251-08pmc: PMC2493223pubmed: 18490466google scholar: lookup
  5. Chen Z, Liu Q, Liu JQ, Xu BL, Lv S, Xia S, Zhou XN. Tick-borne pathogens and associated co-infections in ticks collected from domestic animals in central China.. Parasit Vectors 2014 May 22;7:237.
    doi: 10.1186/1756-3305-7-237pmc: PMC4045914pubmed: 24886497google scholar: lookup
  6. Han YJ, Park J, Lee YS, Chae JS, Yu DH, Park BK, Kim HC, Choi KS. Molecular identification of selected tick-borne pathogens in wild deer and raccoon dogs from the Republic of Korea.. Vet Parasitol Reg Stud Reports 2017 Jan;7:25-31.
    doi: 10.1016/j.vprsr.2016.12.001pubmed: 31014652google scholar: lookup
  7. Orkun Ö, Emir H. Identification of tick-borne pathogens in ticks collected from wild animals in Turkey.. Parasitol Res 2020 Sep;119(9):3083-3091.
    doi: 10.1007/s00436-020-06812-2pubmed: 32683558google scholar: lookup
  8. Labruna MB, Kasai N, Ferreira F, Faccini JL, Gennari SM. Seasonal dynamics of ticks (Acari: Ixodidae) on horses in the state of São Paulo, Brazil.. Vet Parasitol 2002 Apr 19;105(1):65-77.
    doi: 10.1016/S0304-4017(01)00649-5pubmed: 11879967google scholar: lookup
  9. Duell JR, Carmichael R, Herrin BH, Holbrook TC, Talley J, Little SE. Prevalence and species of ticks on horses in central Oklahoma.. J Med Entomol 2013 Nov;50(6):1330-3.
    doi: 10.1603/ME13117pubmed: 24843940google scholar: lookup
  10. Khoury C, Manilla G, Maroli M. [Parasitic horse ticks in Italy. Observations on their distribution and pathogenic role].. Parassitologia 1994 Dec;36(3):273-9.
    pubmed: 7637997
  11. Camacho AT, Guitian FJ, Pallas E, Gestal JJ, Olmeda AS, Habela MA, Telford SR 3rd, Spielman A. Theileria (Babesia) equi and Babesia caballi infections in horses in Galicia, Spain.. Trop Anim Health Prod 2005 May;37(4):293-302.
    doi: 10.1007/s11250-005-5691-zpubmed: 15934637google scholar: lookup
  12. Chan KY, Wang CH, Wu YL. Serological Survey of Equine Piroplasmosis, Equine Granulocytic Anaplasmosis, and Equine Lyme Disease in Taiwan. Taiwan Vet. J. 2010;36:261–267.
  13. Engvall EO, Pettersson B, Persson M, Artursson K, Johansson KE. A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses, and cattle.. J Clin Microbiol 1996 Sep;34(9):2170-4.
    doi: 10.1128/jcm.34.9.2170-2174.1996pmc: PMC229211pubmed: 8862579google scholar: lookup
  14. Teglas M, Matern E, Lein S, Foley P, Mahan SM, Foley J. Ticks and tick-borne disease in Guatemalan cattle and horses.. Vet Parasitol 2005 Jul 15;131(1-2):119-27.
    doi: 10.1016/j.vetpar.2005.04.033pubmed: 15936147google scholar: lookup
  15. Aguero-Rosenfeld ME. Diagnosis of human granulocytic ehrlichiosis: state of the art.. Vector Borne Zoonotic Dis 2002 Winter;2(4):233-9.
    doi: 10.1089/153036602321653815pubmed: 12804164google scholar: lookup
  16. Strle F. Human granulocytic ehrlichiosis in Europe.. Int J Med Microbiol 2004 Apr;293 Suppl 37:27-35.
    doi: 10.1016/S1433-1128(04)80006-8pubmed: 15146982google scholar: lookup
  17. Seo MG, Kwon OD, Kwak D. Diversity and genotypic analysis of tick-borne pathogens carried by ticks infesting horses in Korea.. Med Vet Entomol 2021 Jun;35(2):213-218.
    doi: 10.1111/mve.12483pubmed: 32989737google scholar: lookup
  18. Kim SY, Jeong YE, Yun SM, Lee IY, Han MG, Ju YR. Molecular evidence for tick-borne encephalitis virus in ticks in South Korea.. Med Vet Entomol 2009 Mar;23(1):15-20.
    doi: 10.1111/j.1365-2915.2008.00755.xpubmed: 19239610google scholar: lookup
  19. Lee SO, Na DK, Kim CM, Li YH, Cho YH, Park JH, Lee JH, Eo SK, Klein TA, Chae JS. Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene.. J Vet Sci 2005 Jun;6(2):151-5.
    doi: 10.4142/jvs.2005.6.2.151pubmed: 15933436google scholar: lookup
  20. Seo MG, Kwon OD, Kwak D. Molecular Identification of Borrelia afzelii from Ticks Parasitizing Domestic and Wild Animals in South Korea.. Microorganisms 2020 Apr 29;8(5).
    doi: 10.3390/microorganisms8050649pmc: PMC7284850pubmed: 32365723google scholar: lookup
  21. Im JH, Baek J, Durey A, Kwon HY, Chung MH, Lee JS. Current Status of Tick-Borne Diseases in South Korea.. Vector Borne Zoonotic Dis 2019 Apr;19(4):225-233.
    doi: 10.1089/vbz.2018.2298pubmed: 30328790google scholar: lookup
  22. Lado P, Smith ML, Carstens BC, Klompen H. Population genetic structure and demographic history of the lone star tick, Amblyomma americanum (Ixodida: Ixodidae): New evidence supporting old records.. Mol Ecol 2020 Aug;29(15):2810-2823.
    doi: 10.1111/mec.15524pubmed: 32574413google scholar: lookup
  23. MacDonald AJ. Abiotic and habitat drivers of tick vector abundance, diversity, phenology and human encounter risk in southern California.. PLoS One 2018;13(7):e0201665.
    doi: 10.1371/journal.pone.0201665pmc: PMC6067749pubmed: 30063752google scholar: lookup
  24. Trout Fryxell RT, Moore JE, Collins MD, Kwon Y, Jean-Philippe SR, Schaeffer SM, Odoi A, Kennedy M, Houston AE. Habitat and Vegetation Variables Are Not Enough When Predicting Tick Populations in the Southeastern United States.. PLoS One 2015;10(12):e0144092.
    doi: 10.1371/journal.pone.0144092pmc: PMC4676690pubmed: 26656122google scholar: lookup
  25. Uspensky I. Tick pests and vectors (Acari: Ixodoidea) in European towns: Introduction, persistence and management.. Ticks Tick Borne Dis 2014 Feb;5(1):41-7.
    doi: 10.1016/j.ttbdis.2013.07.011pubmed: 24183576google scholar: lookup
  26. Valcárcel F, González J, González MG, Sánchez M, Tercero JM, Elhachimi L, Carbonell JD, Olmeda AS. Comparative Ecology of Hyalomma lusitanicum and Hyalomma marginatum Koch, 1844 (Acarina: Ixodidae).. Insects 2020 May 13;11(5).
    doi: 10.3390/insects11050303pmc: PMC7290797pubmed: 32414220google scholar: lookup
  27. Kang SW, Doan HT, Choe SE, Noh JH, Yoo MS, Reddy KE, Kim YH, Kweon CH, Jung SC, Chang KY. Molecular investigation of tick-borne pathogens in ticks from grazing cattle in Korea.. Parasitol Int 2013 Jun;62(3):276-82.
    doi: 10.1016/j.parint.2013.02.002pubmed: 23501057google scholar: lookup
  28. Seo H-J, Kim K-H, Lee SK, Min S, Lim J-Y, Yang S-J, Yoo M-S, Jung S, Yoon S-S, Cho YS. Molecular and serological surveillance of equine piroplasmosis in the Republic of Korea between 2016 and 2017. Korean J. Vet. Res. 2021;61:4.
    doi: 10.14405/kjvr.2021.61.e4google scholar: lookup
  29. Sanders DM, Parker JE, Walker WW, Buchholz MW, Blount K, Kiel JL. Field collection and genetic classification of tick-borne Rickettsiae and Rickettsiae-like pathogens from South Texas: Coxiella burnetii isolated from field-collected Amblyomma cajennense.. Ann N Y Acad Sci 2008 Dec;1149:208-11.
    doi: 10.1196/annals.1428.022pubmed: 19120212google scholar: lookup
  30. Noden BH, Martin J, Carrillo Y, Talley JL, Ochoa-Corona FM. Development of a loop-mediated isothermal amplification (LAMP) assay for rapid screening of ticks and fleas for spotted fever group rickettsia.. PLoS One 2018;13(2):e0192331.
    doi: 10.1371/journal.pone.0192331pmc: PMC5794167pubmed: 29390021google scholar: lookup
  31. Boulanger N, Boyer P, Talagrand-Reboul E, Hansmann Y. Ticks and tick-borne diseases.. Med Mal Infect 2019 Mar;49(2):87-97.
    doi: 10.1016/j.medmal.2019.01.007pubmed: 30736991google scholar: lookup
  32. Saleem S, Ijaz M, Farooqi SH, Ghaffar A, Ali A, Iqbal K, Mehmood K, Zhang H. Equine Granulocytic Anaplasmosis 28 years later.. Microb Pathog 2018 Jun;119:1-8.
    doi: 10.1016/j.micpath.2018.04.001pubmed: 29626656google scholar: lookup
  33. Kim CM, Kim MS, Park MS, Park JH, Chae JS. Identification of Ehrlichia chaffeensis, Anaplasma phagocytophilum, and A. bovis in Haemaphysalis longicornis and Ixodes persulcatus ticks from Korea.. Vector Borne Zoonotic Dis 2003 Spring;3(1):17-26.
    doi: 10.1089/153036603765627424pubmed: 12804377google scholar: lookup
  34. Biggs HM, Behravesh CB, Bradley KK, Dahlgren FS, Drexler NA, Dumler JS, Folk SM, Kato CY, Lash RR, Levin ML, Massung RF, Nadelman RB, Nicholson WL, Paddock CD, Pritt BS, Traeger MS. Diagnosis and Management of Tickborne Rickettsial Diseases: Rocky Mountain Spotted Fever and Other Spotted Fever Group Rickettsioses, Ehrlichioses, and Anaplasmosis - United States.. MMWR Recomm Rep 2016 May 13;65(2):1-44.
    doi: 10.15585/mmwr.rr6502a1pubmed: 27172113google scholar: lookup
  35. Lee SH, Yun SH, Choi E, Park YS, Lee SE, Cho GJ, Kwon OD, Kwak D. Serological Detection of Borrelia burgdorferi among Horses in Korea.. Korean J Parasitol 2016 Feb;54(1):97-101.
    doi: 10.3347/kjp.2016.54.1.97pmc: PMC4792323pubmed: 26951987google scholar: lookup
  36. Chomel B. Lyme disease.. Rev Sci Tech 2015 Aug;34(2):569-76.
    doi: 10.20506/rst.34.2.2380pubmed: 26601457google scholar: lookup
  37. Seo MG, Ouh IO, Choi E, Kwon OD, Kwak D. Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Korea.. Korean J Parasitol 2018 Dec;56(6):559-565.
    doi: 10.3347/kjp.2018.56.6.559pmc: PMC6327205pubmed: 30630276google scholar: lookup
  38. Battilani M, De Arcangeli S, Balboni A, Dondi F. Genetic diversity and molecular epidemiology of Anaplasma.. Infect Genet Evol 2017 Apr;49:195-211.
    doi: 10.1016/j.meegid.2017.01.021pubmed: 28122249google scholar: lookup
  39. Margos G, Notter I, Fingerle V. Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods.. Methods Mol Biol 2018;1690:13-33.
    doi: 10.1007/978-1-4939-7383-5_2pubmed: 29032533google scholar: lookup
  40. Urwin R, Maiden MC. Multi-locus sequence typing: a tool for global epidemiology.. Trends Microbiol 2003 Oct;11(10):479-87.
    doi: 10.1016/j.tim.2003.08.006pubmed: 14557031google scholar: lookup
  41. Hoogstraal H, Roberts FH, Kohls GM, Tipton VJ. Review of Haemaphysalis (kaiseriana) Longicornis Neumann (resurrected) of Australia, New Zealand, New Caledonia, Fiji, Japan, Korea, and Northeastern China and USSR, and its parthenogenetic and bisexual populations (Ixodoidea, Ixodidae).. J Parasitol 1968 Dec;54(6):1197-213.
    doi: 10.2307/3276992pubmed: 5757695google scholar: lookup
  42. Hoogstraal H, Wassef HY. The Haemaphysalis ticks (Ixodoidea: Ixodidae) of birds. 3. H. (Ornithophysalis) subgen. n.: definition, species, hosts, and distribution in the Oriental, Palearctic, Malagasy, and Ethiopian faunal regions.. J Parasitol 1973 Dec;59(6):1099-117.
    doi: 10.2307/3278650pubmed: 4760642google scholar: lookup
  43. Yamaguti N, Tipton VJ, Keegan HL, Toshioka S. Ticks of Japan, Korea, and the Ryukyu islands. Brigh. Young Univ. Sci. Bull. Biol. Ser. 1971;15:1–226.
  44. Kramer VL, Randolph MP, Hui LT, Irwin WE, Gutierrez AG, Vugia DJ. Detection of the agents of human ehrlichioses in ixodid ticks from California.. Am J Trop Med Hyg 1999 Jan;60(1):62-5.
    doi: 10.4269/ajtmh.1999.60.62pubmed: 9988324google scholar: lookup
  45. Ott D, Ulrich K, Ginsbach P, Öhme R, Bock-Hensley O, Falk U, Teinert M, Lenhard T. Tick-borne encephalitis virus (TBEV) prevalence in field-collected ticks (Ixodes ricinus) and phylogenetic, structural and virulence analysis in a TBE high-risk endemic area in southwestern Germany.. Parasit Vectors 2020 Jun 11;13(1):303.
    doi: 10.1186/s13071-020-04146-7pmc: PMC7291635pubmed: 32527288google scholar: lookup
  46. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.. Mol Biol Evol 2013 Dec;30(12):2725-9.
    doi: 10.1093/molbev/mst197pmc: PMC3840312pubmed: 24132122google scholar: lookup
  47. Alhassan A, Pumidonming W, Okamura M, Hirata H, Battsetseg B, Fujisaki K, Yokoyama N, Igarashi I. Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood.. Vet Parasitol 2005 Apr 20;129(1-2):43-9.
    doi: 10.1016/j.vetpar.2004.12.018pubmed: 15817201google scholar: lookup
  48. Sparagano OA, Allsopp MT, Mank RA, Rijpkema SG, Figueroa JV, Jongejan F. Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): a review.. Exp Appl Acarol 1999 Dec;23(12):929-60.
    doi: 10.1023/A:1006313803979pubmed: 10737729google scholar: lookup
  49. Hancock SI, Breitschwerdt EB, Pitulle C. Differentiation of Ehrlichia platys and E. equi infections in dogs by using 16S ribosomal DNA-based PCR.. J Clin Microbiol 2001 Dec;39(12):4577-8.
    doi: 10.1128/JCM.39.12.4577-4578.2001pmc: PMC88593pubmed: 11724889google scholar: lookup
  50. Anderson BE, Sumner JW, Dawson JE, Tzianabos T, Greene CR, Olson JG, Fishbein DB, Olsen-Rasmussen M, Holloway BP, George EH. Detection of the etiologic agent of human ehrlichiosis by polymerase chain reaction.. J Clin Microbiol 1992 Apr;30(4):775-80.
    doi: 10.1128/jcm.30.4.775-780.1992pmc: PMC265160pubmed: 1374076google scholar: lookup
  51. Hersh MH, Ostfeld RS, McHenry DJ, Tibbetts M, Brunner JL, Killilea ME, LoGiudice K, Schmidt KA, Keesing F. Co-infection of blacklegged ticks with Babesia microti and Borrelia burgdorferi is higher than expected and acquired from small mammal hosts.. PLoS One 2014;9(6):e99348.
    doi: 10.1371/journal.pone.0099348pmc: PMC4062422pubmed: 24940999google scholar: lookup
  52. Zhai B, Niu Q, Yang J, Liu Z, Liu J, Yin H, Zeng Q. Identification and molecular survey of Borrelia burgdorferi sensu lato in sika deer (Cervus nippon) from Jilin Province, north-eastern China.. Acta Trop 2017 Feb;166:54-57.
    doi: 10.1016/j.actatropica.2016.11.002pubmed: 27818123google scholar: lookup

Citations

This article has been cited 2 times.
  1. Kim YJ, Seo JY, Kim SY, Lee HI. Molecular Detection of Anaplasma phagocytophilum and Ehrlichia Species in Ticks Removed from Humans in the Republic of Korea. Microorganisms 2022 Jun 15;10(6).
    doi: 10.3390/microorganisms10061224pubmed: 35744742google scholar: lookup
  2. Poh KC, Oyen K, Onzere CK, Kappmeyer LS, Bastos RG. Haemaphysalis longicornis ticks are unable to transstadially transmit Theileria haneyi to horses. Front Vet Sci 2025;12:1572944.
    doi: 10.3389/fvets.2025.1572944pubmed: 40241805google scholar: lookup
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