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Home / Equine Research Bank / PLoS One / Muc5b is the major polymeric mucin in mucus from thoroughbre...
PloS one2011; 6(5); e19678; doi: 10.1371/journal.pone.0019678

Muc5b is the major polymeric mucin in mucus from thoroughbred horses with and without airway mucus accumulation.

Abstract: Mucus accumulation is a feature of inflammatory airway disease in the horse and has been associated with reduced performance in racehorses. In this study, we have analysed the two major airways gel-forming mucins Muc5b and Muc5ac in respect of their site of synthesis, their biochemical properties, and their amounts in mucus from healthy horses and from horses with signs of airway mucus accumulation. Polyclonal antisera directed against equine Muc5b and Muc5ac were raised and characterised. Immunohistochemical staining of normal equine trachea showed that Muc5ac and Muc5b are produced by cells in the submucosal glands, as well as surface epithelial goblet cells. Western blotting after agarose gel electrophoresis of airway mucus from healthy horses, and horses with mucus accumulation, was used to determine the amounts of these two mucins in tracheal wash samples. The results showed that in healthy horses Muc5b was the predominant mucin with small amounts of Muc5ac. The amounts of Muc5b and Muc5ac were both dramatically increased in samples collected from horses with high mucus scores as determined visually at the time of endoscopy and that this increase also correlated with increase number of bacteria present in the sample. The change in amount of Muc5b and Muc5ac indicates that Muc5b remains the most abundant mucin in mucus. In summary, we have developed mucin specific polyclonal antibodies, which have allowed us to show that there is a significant increase in Muc5b and Muc5ac in mucus accumulated in equine airways and these increases correlated with the numbers of bacteria.
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Publication Date: 2011-05-13 PubMed ID: 21602926PubMed Central: PMC3094342DOI: 10.1371/journal.pone.0019678Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the association between the presence of two major airway mucins (Muc5b and Muc5ac) and the accumulation of mucus in thoroughbred horses, particularly in relation to inflammatory airway disease. The research shows a significant increase in both types of mucins in horses showing symptoms of mucus accumulation, with Muc5b still being the most prevalent.

Research Methodology

  • The study analyzed the two major airways gel-forming mucins, Muc5b and Muc5ac, in associations to their site of synthesis, biochemical properties, and quantities in mucus from both healthy horses and horses with signs of airway mucus accumulation.
  • To conduct the analysis, polyclonal antisera directed against equine Muc5b and Muc5ac were created and characterized.
  • Immunohistochemical staining of normal equine trachea was performed, which showed that both Muc5ac and Muc5b are produced by cells in the submucosal glands as well as surface epithelial goblet cells.

Key Findings

  • Western blotting after agarose gel electrophoresis of airway mucus from both healthy horses and horses with mucus accumulation was used to determine the amounts of these two mucins in tracheal wash samples.
  • The results indicated that in healthy horses, Muc5b was the dominant mucin present with small amounts of Muc5ac.
  • However, in samples collected from horses with high mucus scores as determined visually at the time of endoscopy, the amounts of both Muc5b and Muc5ac dramatically increases. This increase also correlated with the number of bacteria present in the sample.
  • This change indicates that although the amount of Muc5ac increases, Muc5b remains the most abundant mucin in mucus.

Conclusion

  • Through the use of specifically developed mucin specific polyclonal antibodies, the researchers were able to illustrate that there is a significant increase in both Muc5b and Muc5ac in mucus accumulating in equine airways.
  • This increase in the two types of mucins directly corresponds with the number of bacteria present in a given sample, thus serving as a potential indicator for inflammatory airway disease in racehorses.

Cite This Article

APA
Rousseau K, Cardwell JM, Humphrey E, Newton R, Knight D, Clegg P, Thornton DJ. (2011). Muc5b is the major polymeric mucin in mucus from thoroughbred horses with and without airway mucus accumulation. PLoS One, 6(5), e19678. https://doi.org/10.1371/journal.pone.0019678

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 6
Issue: 5
Pages: e19678
PII: e19678

Researcher Affiliations

Rousseau, Karine
  • Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.
Cardwell, Jacqueline M
    Humphrey, Emma
      Newton, Richard
        Knight, David
          Clegg, Peter
            Thornton, David J

              MeSH Terms

              • Airway Obstruction / microbiology
              • Airway Obstruction / veterinary
              • Animals
              • Antibodies
              • Bacteria
              • Horse Diseases / diagnosis
              • Horses
              • Immune Sera
              • Inflammation / microbiology
              • Inflammation / veterinary
              • Mucin-5B / analysis
              • Mucin-5B / immunology
              • Mucins / analysis
              • Mucins / immunology
              • Mucus / chemistry

              Conflict of Interest Statement

              The authors have declared that no competing interests exist.

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              Citations

              This article has been cited 1 times.
              1. Ridley C, Kirkham S, Williamson SJ, Davis CW, Woodman P, Thornton DJ. Biosynthesis of the polymeric gel-forming mucin MUC5B.. Am J Physiol Lung Cell Mol Physiol 2016 May 15;310(10):L993-L1002.
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