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Home / Equine Research Bank / J Tissue Eng Regen Med / Novel nanostructured scaffold for osteochondral regeneration...
Journal of tissue engineering and regenerative medicine2010; 4(4); 300-308; doi: 10.1002/term.243

Novel nanostructured scaffold for osteochondral regeneration: pilot study in horses.

Abstract: The present in vivo preliminary experiment is aimed at testing mechanical and biological behaviour of a new nano-structured composite multilayer biomimetic scaffold for the treatment of chondral and osteochondral defects. The three-dimensional biomimetic scaffold (Fin-Ceramica Faenza S.p.A., Faenza-Italy) was obtained by nucleating collagen fibrils with hydroxyapatite nanoparticles, in two configurations, bi- and tri-layered, to reproduce, respectively, chondral and osteochondral anatomy. Chondral defects (lateral condyle) and deep osteochondral defects (medial condyle) were made in the distal epiphysis of the third metacarpal bone of both forelimbs of two adult horses and treated respectively with the chondral and osteochondral grafts. Both animals were euthanised six months follow up. The images obtained at the second look arthroscopy evaluation, performed two months after surgery, demonstrated good filling of the chondral and osteo-chondral defects without any inflammatory reaction around and inside the lesions. At the histological analysis the growth of trabecular bone in the osteochondral lesion was evident. Only in one case, the whole thickness of the osteochondral lesion was filled by fibrocartilaginous tissue. The formation of a tidemark line was evident at the interface with the newly formed bone. Newly formed fibrocartilaginous tissue was present in the area of the chondral defect. Initial alignment of the collagen fibres was recognisable with polarised light in both groups. The results of the present pilot study showed that this novel osteochondral and chondral scaffold may act as a suitable matrix to facilitate orderly regeneration of bone and hyaline-like cartilage.
Copyright 2010 John Wiley & Sons, Ltd.
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Publication Date: 2010-01-06 PubMed ID: 20049745DOI: 10.1002/term.243Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers conducted a study on a new nano-structured scaffold intended to heal bone and cartilage defects. The study, conducted on horses, disclosed promising results with the scaffold showing potential to facilitate orderly regeneration of bone and cartilage.

Objective of the Research

  • The aim of this study was to assess the mechanical and biological efficacy of a novel multilayer biomimetic scaffold designed for the treatment of chondral (cartilage) and osteochondral (bone and cartilage) defects.

Methods

  • The scaffold product, obtained from Fin-Ceramica Faenza S.p.A., was created using collagen fibrils and hydroxyapatite nanoparticles. It was constructed in two different configurations: bi-layered for chondral anatomy and tri-layered for osteochondral anatomy.
  • Both chondral and osteochondral defects were artificially created on the distal epiphysis of the third metacarpal bone in the forelimbs of two adult horses. These defects were then treated with the respective grafts.
  • Post-operative assessments including arthroscopy evaluations and histological analyses were performed at different time intervals. The horses were euthanised after six months for the purpose of this study.

Results

  • Arthroscopy evaluations two months post-surgery showed satisfactory filling of the chondral and osteochondral defects. No inflammatory reaction was observed around or inside the affected areas.
  • Upon histological analysis, there was apparent growth of trabecular bone in the osteochondral lesion. Only in one case was the whole thickness of the osteochondral lesion filled with fibrocartilaginous tissue.
  • A distinct tidemark line (a line that marks the boundary between calcified and uncalcified cartilage) was evident at the interface with the regenerated bone. Newly formed fibrocartilaginous tissue was also present in the chondral defect zone.
  • The initial alignment of collagen fibres in both defect types was recognisable under polarised light.

Conclusion

  • This pilot study suggested that the novel osteochondral and chondral scaffold may serve as a proper matrix for orderly bone and cartilage regeneration. Therefore, this novel scaffold offers potential benefits in treating bone and cartilage defects.

Cite This Article

APA
Kon E, Muttini A, Arcangeli E, Delcogliano M, Filardo G, Nicoli Aldini N, Pressato D, Quarto R, Zaffagnini S, Marcacci M. (2010). Novel nanostructured scaffold for osteochondral regeneration: pilot study in horses. J Tissue Eng Regen Med, 4(4), 300-308. https://doi.org/10.1002/term.243

Publication

Journal of tissue engineering and regenerative medicine
ISSN: 1932-7005
NlmUniqueID: 101308490
Country: England
Language: English
Volume: 4
Issue: 4
Pages: 300-308

Researcher Affiliations

Kon, E
  • Rizzoli Orthopaedic Instituite, Bologna, Italy.
Muttini, A
    Arcangeli, E
      Delcogliano, M
        Filardo, G
          Nicoli Aldini, N
            Pressato, D
              Quarto, R
                Zaffagnini, S
                  Marcacci, M

                    MeSH Terms

                    • Animals
                    • Arthroscopy
                    • Bone and Bones / cytology
                    • Bone and Bones / pathology
                    • Bone and Bones / physiology
                    • Bone and Bones / surgery
                    • Chondrocytes / cytology
                    • Chondrocytes / pathology
                    • Follow-Up Studies
                    • Horses
                    • Joints / pathology
                    • Joints / surgery
                    • Nanostructures / chemistry
                    • Pilot Projects
                    • Regeneration
                    • Tissue Scaffolds / chemistry

                    Citations

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