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Home / Equine Research Bank / PLoS One / Oral Administration of Electron-Beam Inactivated Rhodococcus...
PloS one2016; 11(2); e0148111; doi: 10.1371/journal.pone.0148111

Oral Administration of Electron-Beam Inactivated Rhodococcus equi Failed to Protect Foals against Intrabronchial Infection with Live, Virulent R. equi.

Abstract: There is currently no licensed vaccine that protects foals against Rhodococcus equi-induced pneumonia. Oral administration of live, virulent R. equi to neonatal foals has been demonstrated to protect against subsequent intrabronchial challenge with virulent R. equi. Electron beam (eBeam)-inactivated R. equi are structurally intact and have been demonstrated to be immunogenic when administered orally to neonatal foals. Thus, we investigated whether eBeam inactivated R. equi could protect foals against developing pneumonia after experimental infection with live, virulent R. equi. Foals (n = 8) were vaccinated by gavaging with eBeam-inactivated R. equi at ages 2, 7, and 14 days, or gavaged with equal volume of saline solution (n = 4), and subsequently infected intrabronchially with live, virulent R. equi at age 21 days. The proportion of vaccinated foals that developed pneumonia following challenge was similar among the vaccinated (7/8; 88%) and unvaccinated foals (3/4; 75%). This vaccination regimen did not appear to be strongly immunogenic in foals. Alternative dosing regimens or routes of administration need further investigation and may prove to be immunogenic and protective.
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Publication Date: 2016-02-01 PubMed ID: 26828865PubMed Central: PMC4735123DOI: 10.1371/journal.pone.0148111Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This article presents a study which investigates the effectiveness of a vaccination method against Rhodococcus equi-induced pneumonia in foals. However, the researchers found that the treatment, which involved orally administering inactive R. equi, failed to offer substantial protection against infection with live, virulent R. equi.

Introduction and Objective of the Study

  • The study aimed to evaluate the effectiveness of a potential vaccination against Rhodococcus equi, a bacterium causing serious pneumonia in foals.
  • There is no existing licensed vaccine for this disease, and the researchers explored the efficacy of orally administering an electron beam (eBeam)-inactivated form of R. equi, shown to provoke an immune response in previous studies.

Methodology and Results

  • Eight foals were given the eBeam-inactivated R. equi through a method known as gavage (orally) at 2, 7, and 14 days old. Additionally, four foals were given saline solution by the same method as a control group.
  • At 21 days old, all foals were infected intrabronchially (directly into the bronchi of the lungs) with live, virulent R. equi to test the broadly preventable effect of the earlier vaccine regimen.
  • The results showed a high percentage of vaccinated foals developing pneumonia (7 out of 8, or 88%) while 75% (3 out of 4) unvaccinated foals also developed pneumonia.

Interpretation and Conclusion

  • The fact that both vaccinated and unvaccinated foals notably developed pneumonia after being infected suggests the vaccination regimen may not be significantly immunogenic, meaning it does not sufficiently stimulate an immune response. Therefore, it appears to be ineffective in preventing R. equi pneumonia in these circumstances.
  • The authors advise further investigation into other potential vaccination dosing plans or administration methods that could prove more successful in inducing immunity and protection against Rhodococcus equi-induced pneumonia in foals.

Cite This Article

APA
Rocha JN, Cohen ND, Bordin AI, Brake CN, Giguère S, Coleman MC, Alaniz RC, Lawhon SD, Mwangi W, Pillai SD. (2016). Oral Administration of Electron-Beam Inactivated Rhodococcus equi Failed to Protect Foals against Intrabronchial Infection with Live, Virulent R. equi. PLoS One, 11(2), e0148111. https://doi.org/10.1371/journal.pone.0148111

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 11
Issue: 2
Pages: e0148111
PII: e0148111

Researcher Affiliations

Rocha, Joana N
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, 77843-4475, United States of America.
Cohen, Noah D
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, 77843-4475, United States of America.
Bordin, Angela I
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, 77843-4475, United States of America.
Brake, Courtney N
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, 77843-4475, United States of America.
Giguère, Steeve
  • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, 30602-7385, United States of America.
Coleman, Michelle C
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, 77843-4475, United States of America.
Alaniz, Robert C
  • Department of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, College Station, Texas, 77843, United States of America.
Lawhon, Sara D
  • Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, 77843-4467, United States of America.
Mwangi, Waithaka
  • Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, 77843-4467, United States of America.
Pillai, Suresh D
  • National Center for Electron Beam Research-IAEA Collaborative Centre for Electron Beam Technology, Texas A&M University, College Station, Texas, 77843, United States of America.

MeSH Terms

  • Actinomycetales Infections / diagnostic imaging
  • Actinomycetales Infections / veterinary
  • Administration, Oral
  • Animals
  • Antibody Formation / immunology
  • Antibody Specificity / immunology
  • Bronchi / microbiology
  • Electrons
  • Horse Diseases / diagnostic imaging
  • Horse Diseases / immunology
  • Horses
  • Immunity, Cellular
  • Immunoglobulin G / metabolism
  • Interferon-gamma / biosynthesis
  • Leukocytes, Mononuclear / metabolism
  • Nose / immunology
  • Rhodococcus equi / physiology
  • Treatment Outcome
  • Ultrasonography
  • Vaccination / veterinary
  • Virulence

Conflict of Interest Statement

The authors have declared that no competing interests exist.

References

This article includes 57 references
  1. Takai S. Epidemiology of Rhodococcus equi infections: a review.. Vet Microbiol 1997 Jun 16;56(3-4):167-76.
    pubmed: 9226831doi: 10.1016/s0378-1135(97)00085-0google scholar: lookup
  2. Giguère S, Prescott JF. Clinical manifestations, diagnosis, treatment, and prevention of Rhodococcus equi infections in foals.. Vet Microbiol 1997 Jun 16;56(3-4):313-34.
    pubmed: 9226845doi: 10.1016/s0378-1135(97)00099-0google scholar: lookup
  3. Giguère S, Hondalus MK, Yager JA, Darrah P, Mosser DM, Prescott JF. Role of the 85-kilobase plasmid and plasmid-encoded virulence-associated protein A in intracellular survival and virulence of Rhodococcus equi.. Infect Immun 1999 Jul;67(7):3548-57.
    pmc: PMC116543pubmed: 10377138doi: 10.1128/iai.67.7.3548-3557.1999google scholar: lookup
  4. Takai S, Sekizaki T, Ozawa T, Sugawara T, Watanabe Y, Tsubaki S. Association between a large plasmid and 15- to 17-kilodalton antigens in virulent Rhodococcus equi.. Infect Immun 1991 Nov;59(11):4056-60.
    pmc: PMC258996pubmed: 1937765doi: 10.1128/iai.59.11.4056-4060.1991google scholar: lookup
  5. Tkachuk-Saad O, Prescott J. Rhodococcus equi plasmids: isolation and partial characterization.. J Clin Microbiol 1991 Dec;29(12):2696-700.
    pmc: PMC270416pubmed: 1757535doi: 10.1128/jcm.29.12.2696-2700.1991google scholar: lookup
  6. Prescott JF. Rhodococcus equi: an animal and human pathogen.. Clin Microbiol Rev 1991 Jan;4(1):20-34.
    pmc: PMC358176pubmed: 2004346doi: 10.1128/cmr.4.1.20google scholar: lookup
  7. Witonsky S. CVID in a horse with chronic peritonitis: Case review and overview of other immunodeficiencies. Equine Veterinary Education 22: 400–402.
  8. Giguère S, Cohen ND, Chaffin MK, Hines SA, Hondalus MK, Prescott JF, Slovis NM. Rhodococcus equi: clinical manifestations, virulence, and immunity.. J Vet Intern Med 2011 Nov-Dec;25(6):1221-30.
    doi: 10.1111/j.1939-1676.2011.00804.xpubmed: 22092609google scholar: lookup
  9. Venner M, Meyer-Hamme B, Verspohl J, Hatori F, Shimizu N, Sasaki Y, Kakuda T, Tsubaki S, Takai S. Genotypic characterization of VapA positive Rhodococcus equi in foals with pulmonary affection and their soil environment on a warmblood horse breeding farm in Germany.. Res Vet Sci 2007 Dec;83(3):311-7.
    pubmed: 17360011doi: 10.1016/j.rvsc.2007.01.009google scholar: lookup
  10. Muscatello G. Rhodococcus equi pneumonia in the foal--part 1: pathogenesis and epidemiology.. Vet J 2012 Apr;192(1):20-6.
    doi: 10.1016/j.tvjl.2011.08.014pubmed: 22015138google scholar: lookup
  11. Woolcock JB, Mutimer MD, Bowles PM. The immunological response of foals to Rhodococcus equi: a review.. Vet Microbiol 1987 Aug;14(3):215-24.
    pubmed: 3314107doi: 10.1016/0378-1135(87)90108-8google scholar: lookup
  12. Giguère S, Cohen ND, Chaffin MK, Hines SA, Hondalus MK, Prescott JF, Slovis NM. Rhodococcus equi: clinical manifestations, virulence, and immunity.. J Vet Intern Med 2011 Nov-Dec;25(6):1221-30.
    doi: 10.1111/j.1939-1676.2011.00804.xpubmed: 22092609google scholar: lookup
  13. Giguère S, Hernandez J, Gaskin J, Miller C, Bowman JL. Evaluation of white blood cell concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion test for early identification of foals with Rhodococcus equi pneumonia.. J Am Vet Med Assoc 2003 Mar 15;222(6):775-81.
    pubmed: 12675301doi: 10.2460/javma.2003.222.775google scholar: lookup
  14. Chaffin MK. Evaluation of hematologic screening methods for predicting subsequent onset of clinically-apparent Rhodococcus equi pneumonia in foals. In: 59th Annual Convention of the American Association of Equine Practitioners, Nashville, Tennessee, USA, p 267.
  15. Takai S, Sasaki Y, Tsubaki S. Rhodococcus equi infection in foals–current concepts and implications for future research. J Equine Sci 4: 105–119.
  16. Cohen ND, Smith KE, Ficht TA, Takai S, Libal MC, West BR, DelRosario LS, Becu T, Leadon DP, Buckley T, Chaffin MK, Martens RJ. Epidemiologic study of results of pulsed-field gel electrophoresis of isolates of Rhodococcus equi obtained from horses and horse farms.. Am J Vet Res 2003 Feb;64(2):153-61.
    pubmed: 12602583doi: 10.2460/ajvr.2003.64.153google scholar: lookup
  17. Donecker JM, Holland RE. Efficacy of the Immunomodulator Zylexis in horses challenged with equine herpes-virus under field conditions. Pfizer Animal Health.
  18. Chaffin MK, Cohen ND, Martens RJ. Chemoprophylactic effects of azithromycin against Rhodococcus equi-induced pneumonia among foals at equine breeding farms with endemic infections.. J Am Vet Med Assoc 2008 Apr 1;232(7):1035-47.
    doi: 10.2460/javma.232.7.1035pubmed: 18380623google scholar: lookup
  19. Madigan JE, Hietala S, Muller N. Protection against naturally acquired Rhodococcus equi pneumonia in foals by administration of hyperimmune plasma.. J Reprod Fertil Suppl 1991;44:571-8.
    pubmed: 1795301
  20. Caston SS, McClure SR, Martens RJ, Chaffin MK, Miles KG, Griffith RW, Cohen ND. Effect of hyperimmune plasma on the severity of pneumonia caused by Rhodococcus equi in experimentally infected foals.. Vet Ther 2006 Winter;7(4):361-75.
    pubmed: 17216591
  21. Martens RJ, Martens JG, Fiske RA, Hietala SK. Rhodococcus equi foal pneumonia: protective effects of immune plasma in experimentally infected foals.. Equine Vet J 1989 Jul;21(4):249-55.
    pubmed: 2767025doi: 10.1111/j.2042-3306.1989.tb02161.xgoogle scholar: lookup
  22. Wilson EM, Holcombe SJ, Lamar A, Hauptman JG, Brooks MB. Incidence of transfusion reactions and retention of procoagulant and anticoagulant factor activities in equine plasma.. J Vet Intern Med 2009 Mar-Apr;23(2):323-8.
    pubmed: 19192141doi: 10.1111/j.1939-1676.2008.0254.xgoogle scholar: lookup
  23. Giguère S, Gaskin JM, Miller C, Bowman JL. Evaluation of a commercially available hyperimmune plasma product for prevention of naturally acquired pneumonia caused by Rhodococcus equi in foals.. J Am Vet Med Assoc 2002 Jan 1;220(1):59-63.
    pubmed: 12680449doi: 10.2460/javma.2002.220.59google scholar: lookup
  24. Martens RJ, Martens JG, Fiske RA. Failure of passive immunization by colostrum from immunized mares to protect foals against Rhodococcus equi pneumonia. Equine Vet J 23: 19–22.
  25. Cauchard J, Sevin C, Ballet JJ, Taouji S. Foal IgG and opsonizing anti-Rhodococcus equi antibodies after immunization of pregnant mares with a protective VapA candidate vaccine.. Vet Microbiol 2004 Nov 30;104(1-2):73-81.
    pubmed: 15530741doi: 10.1016/j.vetmic.2004.09.006google scholar: lookup
  26. Becú T, Polledo G, Gaskin JM. Immunoprophylaxis of Rhodococcus equi pneumonia in foals.. Vet Microbiol 1997 Jun 16;56(3-4):193-204.
    pubmed: 9226834doi: 10.1016/s0378-1135(97)00088-6google scholar: lookup
  27. Prescott JF, Nicholson VM, Patterson MC, Zandona Meleiro MC, Caterino de Araujo A, Yager JA, Holmes MA. Use of Rhodococcus equi virulence-associated protein for immunization of foals against R equi pneumonia.. Am J Vet Res 1997 Apr;58(4):356-9.
    pubmed: 9099378
  28. Kohler AK, Stone DM, Hines MT, Byrne BA, Alperin DC, Norton LK, Hines SA. Rhodococcus equi secreted antigens are immunogenic and stimulate a type 1 recall response in the lungs of horses immune to R. equi infection.. Infect Immun 2003 Nov;71(11):6329-37.
    pmc: PMC219552pubmed: 14573652doi: 10.1128/iai.71.11.6329-6337.2003google scholar: lookup
  29. Ashour J, Hondalus MK. Phenotypic mutants of the intracellular actinomycete Rhodococcus equi created by in vivo Himar1 transposon mutagenesis.. J Bacteriol 2003 Apr;185(8):2644-52.
    pmc: PMC152612pubmed: 12670990doi: 10.1128/jb.185.8.2644-2652.2003google scholar: lookup
  30. Pei Y, Nicholson V, Woods K, Prescott JF. Immunization by intrabronchial administration to 1-week-old foals of an unmarked double gene disruption strain of Rhodococcus equi strain 103+.. Vet Microbiol 2007 Nov 15;125(1-2):100-10.
    pubmed: 17560744doi: 10.1016/j.vetmic.2007.05.007google scholar: lookup
  31. Mealey RH, Stone DM, Hines MT, Alperin DC, Littke MH, Leib SR, Leach SE, Hines SA. Experimental Rhodococcus equi and equine infectious anemia virus DNA vaccination in adult and neonatal horses: effect of IL-12, dose, and route.. Vaccine 2007 Oct 23;25(43):7582-97.
    pmc: PMC3342688pubmed: 17889970doi: 10.1016/j.vaccine.2007.07.055google scholar: lookup
  32. Phumoonna T, Barton MD, Vanniasinkam T, Heuzenroeder MW. Chimeric vapA/groEL2 DNA vaccines enhance clearance of Rhodococcus equi in aerosol challenged C3H/He mice.. Vaccine 2008 May 12;26(20):2457-65.
    doi: 10.1016/j.vaccine.2008.03.015pubmed: 18423949google scholar: lookup
  33. Giles C, Vanniasinkam T, Ndi S, Barton MD. Rhodococcus equi (Prescottella equi) vaccines; the future of vaccine development.. Equine Vet J 2015 Sep;47(5):510-8.
    doi: 10.1111/evj.12310pubmed: 24945608google scholar: lookup
  34. Chirino-Trejo JM, Prescott JF, Yager JA. Protection of foals against experimental Rhodococcus equi pneumonia by oral immunization.. Can J Vet Res 1987 Oct;51(4):444-7.
    pmc: PMC1255362pubmed: 3453264
  35. Hooper-McGrevy KE, Wilkie BN, Prescott JF. Virulence-associated protein-specific serum immunoglobulin G-isotype expression in young foals protected against Rhodococcus equi pneumonia by oral immunization with virulent R. equi.. Vaccine 2005 Dec 30;23(50):5760-7.
    pubmed: 16112256doi: 10.1016/j.vaccine.2005.07.050google scholar: lookup
  36. Bordin AI, Pillai SD, Brake C, Bagley KB, Bourquin JR, Coleman M, Oliveira FN, Mwangi W, McMurray DN, Love CC, Felippe MJ, Cohen ND. Immunogenicity of an electron beam inactivated Rhodococcus equi vaccine in neonatal foals.. PLoS One 2014;9(8):e105367.
    doi: 10.1371/journal.pone.0105367pmc: PMC4143214pubmed: 25153708google scholar: lookup
  37. Chauhan OP, Unni LE. Pulsed electric field (PEF) processing of foods and its combination with electron beam processing. In: Pillai S, Shayanfar S, editors. Electron beam pasteurization and complementary food processing technologies. Cambridge: Woodland Publishing; 2015. p. 157–168.
  38. Burton AJ, Giguère S, Arnold RD. Pharmacokinetics, pulmonary disposition and tolerability of liposomal gentamicin and free gentamicin in foals.. Equine Vet J 2015 Jul;47(4):467-72.
    doi: 10.1111/evj.12309pubmed: 24943347google scholar: lookup
  39. Lopez AM, Hines MT, Palmer GH, Alperin DC, Hines SA. Identification of pulmonary T-lymphocyte and serum antibody isotype responses associated with protection against Rhodococcus equi.. Clin Diagn Lab Immunol 2002 Nov;9(6):1270-6.
    pmc: PMC130094pubmed: 12414760doi: 10.1128/cdli.9.6.1270-1276.2002google scholar: lookup
  40. Sanz M, Loynachan A, Sun L, Oliveira A, Breheny P, Horohov DW. The effect of bacterial dose and foal age at challenge on Rhodococcus equi infection.. Vet Microbiol 2013 Dec 27;167(3-4):623-31.
    doi: 10.1016/j.vetmic.2013.09.018pubmed: 24139178google scholar: lookup
  41. Jacks S, Giguère S, Crawford PC, Castleman WL. Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.. Clin Vaccine Immunol 2007 Jun;14(6):669-77.
    pmc: PMC1951072pubmed: 17409222doi: 10.1128/cvi.00042-07google scholar: lookup
  42. Venner M, Rödiger A, Laemmer M, Giguère S. Failure of antimicrobial therapy to accelerate spontaneous healing of subclinical pulmonary abscesses on a farm with endemic infections caused by Rhodococcus equi.. Vet J 2012 Jun;192(3):293-8.
    doi: 10.1016/j.tvjl.2011.07.004pubmed: 21924651google scholar: lookup
  43. McQueen CM, Doan R, Dindot SV, Bourquin JR, Zlatev ZZ, Chaffin MK, Blodgett GP, Ivanov I, Cohen ND. Identification of genomic loci associated with Rhodococcus equi susceptibility in foals.. PLoS One 2014;9(6):e98710.
    doi: 10.1371/journal.pone.0098710pmc: PMC4043894pubmed: 24892408google scholar: lookup
  44. Sedlinská M, Krejčí J, Vyskočil M, Kudláčková H. Postnatal development of blood serum concentrations of immunoglobulin IgG, IgA and IgM isotypes in suckling foal. Acta Vet Brno 75:175–182.
  45. Sanz MG, Oliveira AF, Loynachan A, Page A, Svansson V, Giguère S, Horohov DW. Validation and evaluation of VapA-specific IgG and IgG subclass enzyme-linked immunosorbent assays (ELISAs) to identify foals with Rhodococcus equi pneumonia.. Equine Vet J 2016 Jan;48(1):103-8.
    doi: 10.1111/evj.12363pubmed: 25257622google scholar: lookup
  46. Lewis MJ, Wagner B, Woof JM. The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.. Mol Immunol 2008 Feb;45(3):818-27.
    pmc: PMC2075531pubmed: 17669496doi: 10.1016/j.molimm.2007.06.158google scholar: lookup
  47. Sanz MG, Villarino N, Ferreira-Oliveira A, Horohov DW. VapA-specific IgG and IgG subclasses responses after natural infection and experimental challenge of foals with Rhodococcus equi.. Vet Immunol Immunopathol 2015 Mar 15;164(1-2):10-5.
    pubmed: 25681111doi: 10.1016/j.vetimm.2015.01.004google scholar: lookup
  48. Perkins GA, Wagner B. The development of equine immunity: Current knowledge on immunology in the young horse.. Equine Vet J 2015 May;47(3):267-74.
    doi: 10.1111/evj.12387pubmed: 25405920google scholar: lookup
  49. Joller N, Weber SS, Müller AJ, Spörri R, Selchow P, Sander P, Hilbi H, Oxenius A. Antibodies protect against intracellular bacteria by Fc receptor-mediated lysosomal targeting.. Proc Natl Acad Sci U S A 2010 Nov 23;107(47):20441-6.
    doi: 10.1073/pnas.1013827107pmc: PMC2996673pubmed: 21048081google scholar: lookup
  50. Hooper-McGrevy KE, Wilkie BN, Prescott JF. Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins.. Clin Diagn Lab Immunol 2003 May;10(3):345-51.
    pmc: PMC154967pubmed: 12738629doi: 10.1128/cdli.10.3.345-351.2003google scholar: lookup
  51. Bordin AI, Liu M, Nerren JR, Buntain SL, Brake CN, Kogut MH, Cohen ND. Neutrophil function of neonatal foals is enhanced in vitro by CpG oligodeoxynucleotide stimulation.. Vet Immunol Immunopathol 2012 Jan 15;145(1-2):290-7.
    pubmed: 22197007doi: 10.1016/j.vetimm.2011.11.012google scholar: lookup
  52. Boyd NK, Cohen ND, Lim WS, Martens RJ, Chaffin MK, Ball JM. Temporal changes in cytokine expression of foals during the first month of life.. Vet Immunol Immunopathol 2003 Mar 20;92(1-2):75-85.
    pubmed: 12628765doi: 10.1016/s0165-2427(03)00021-7google scholar: lookup
  53. Sturgill TL, Giguère S, Berghaus LJ, Hurley DJ, Hondalus MK. Comparison of antibody and cell-mediated immune responses of foals and adult horses after vaccination with live Mycobacterium bovis BCG.. Vaccine 2014 Mar 10;32(12):1362-7.
    doi: 10.1016/j.vaccine.2014.01.032pubmed: 24486362google scholar: lookup
  54. Breathnach CC, Sturgill-Wright T, Stiltner JL, Adams AA, Lunn DP, Horohov DW. Foals are interferon gamma-deficient at birth.. Vet Immunol Immunopathol 2006 Aug 15;112(3-4):199-209.
    pubmed: 16621024doi: 10.1016/j.vetimm.2006.02.010google scholar: lookup
  55. Sturgill TL, Strong D, Rashid C, Betancourt A, Horohov DW. Effect of Propionibacterium acnes-containing immunostimulant on interferon-gamma (IFNγ) production in the neonatal foal.. Vet Immunol Immunopathol 2011 May 15;141(1-2):124-7.
    doi: 10.1016/j.vetimm.2011.01.015pubmed: 21382640google scholar: lookup
  56. Ryan C, Giguère S. Equine neonates have attenuated humoral and cell-mediated immune responses to a killed adjuvanted vaccine compared to adult horses.. Clin Vaccine Immunol 2010 Dec;17(12):1896-902.
    doi: 10.1128/CVI.00328-10pmc: PMC3008191pubmed: 20943883google scholar: lookup
  57. Hurley JR, Begg AP. Failure of hyperimmune plasma to prevent pneumonia caused by Rhodococcus equi in foals.. Aust Vet J 1995 Nov;72(11):418-20.
    pubmed: 8929188doi: 10.1111/j.1751-0813.1995.tb06192.xgoogle scholar: lookup

Citations

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  1. Liu L, Yu W, Cai K, Ma S, Wang Y, Ma Y, Zhao H. Identification of vaccine candidates against rhodococcus equi by combining pangenome analysis with a reverse vaccinology approach.. Heliyon 2023 Aug;9(8):e18623.
    doi: 10.1016/j.heliyon.2023.e18623pubmed: 37576287google scholar: lookup
  2. Cohen ND, Kahn SK, Bordin AI, Gonzales GM, da Silveira BP, Bray JM, Legere RM, Ramirez-Cortez SC. Association of pneumonia with concentrations of virulent Rhodococcus equi in fecal swabs of foals before and after intrabronchial infection with virulent R. equi.. J Vet Intern Med 2022 May;36(3):1139-1145.
    doi: 10.1111/jvim.16409pubmed: 35322902google scholar: lookup
  3. Bhatia SS, Pillai SD. Ionizing Radiation Technologies for Vaccine Development - A Mini Review.. Front Immunol 2022;13:845514.
    doi: 10.3389/fimmu.2022.845514pubmed: 35222438google scholar: lookup
  4. Kahn SK, Cywes-Bentley C, Blodgett GP, Canaday NM, Turner-Garcia CE, Flores-Ahlschwede P, Metcalfe LL, Nevill M, Vinacur M, Sutter PJ, Meyer SC, Bordin AI, Pier GB, Cohen ND. Randomized, controlled trial comparing Rhodococcus equi and poly-N-acetyl glucosamine hyperimmune plasma to prevent R equi pneumonia in foals.. J Vet Intern Med 2021 Nov;35(6):2912-2919.
    doi: 10.1111/jvim.16294pubmed: 34738651google scholar: lookup
  5. Vail KJ, da Silveira BP, Bell SL, Cohen ND, Bordin AI, Patrick KL, Watson RO. The opportunistic intracellular bacterial pathogen Rhodococcus equi elicits type I interferon by engaging cytosolic DNA sensing in macrophages.. PLoS Pathog 2021 Sep;17(9):e1009888.
    doi: 10.1371/journal.ppat.1009888pubmed: 34473814google scholar: lookup
  6. Kahn SK, Cywes-Bentley C, Blodgett GP, Canaday NM, Turner-Garcia CE, Vinacur M, Cortez-Ramirez SC, Sutter PJ, Meyer SC, Bordin AI, Vlock DR, Pier GB, Cohen ND. Antibody activities in hyperimmune plasma against the Rhodococcus equi virulence -associated protein A or poly-N-acetyl glucosamine are associated with protection of foals against rhodococcal pneumonia.. PLoS One 2021;16(8):e0250133.
    doi: 10.1371/journal.pone.0250133pubmed: 34437551google scholar: lookup
  7. Cohen ND, Kahn SK, Cywes-Bentley C, Ramirez-Cortez S, Schuckert AE, Vinacur M, Bordin AI, Pier GB. Serum Antibody Activity against Poly-N-Acetyl Glucosamine (PNAG), but Not PNAG Vaccination Status, Is Associated with Protecting Newborn Foals against Intrabronchial Infection with Rhodococcus equi.. Microbiol Spectr 2021 Sep 3;9(1):e0063821.
    doi: 10.1128/Spectrum.00638-21pubmed: 34319137google scholar: lookup
  8. Bordin AI, Cohen ND, Giguère S, Bray JM, Berghaus LJ, Scott B, Johnson R, Hook M. Host-directed therapy in foals can enhance functional innate immunity and reduce severity of Rhodococcus equi pneumonia.. Sci Rep 2021 Jan 28;11(1):2483.
    doi: 10.1038/s41598-021-82049-ypubmed: 33510265google scholar: lookup
  9. Rakowska A, Cywinska A, Witkowski L. Current Trends in Understanding and Managing Equine Rhodococcosis.. Animals (Basel) 2020 Oct 18;10(10).
    doi: 10.3390/ani10101910pubmed: 33081047google scholar: lookup
  10. Folmar CN, Cywes-Bentley C, Bordin AI, Rocha JN, Bray JM, Kahn SK, Schuckert AE, Pier GB, Cohen ND. In vitro evaluation of complement deposition and opsonophagocytic killing of Rhodococcus equi mediated by poly-N-acetyl glucosamine hyperimmune plasma compared to commercial plasma products.. J Vet Intern Med 2019 May;33(3):1493-1499.
    doi: 10.1111/jvim.15511pubmed: 31034109google scholar: lookup
  11. Cywes-Bentley C, Rocha JN, Bordin AI, Vinacur M, Rehman S, Zaidi TS, Meyer M, Anthony S, Lambert M, Vlock DR, Giguère S, Cohen ND, Pier GB. Antibody to Poly-N-acetyl glucosamine provides protection against intracellular pathogens: Mechanism of action and validation in horse foals challenged with Rhodococcus equi.. PLoS Pathog 2018 Jul;14(7):e1007160.
    doi: 10.1371/journal.ppat.1007160pubmed: 30024986google scholar: lookup
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