Preliminary studies on long distance, retrograde transport of horseradish peroxidase in equine peripheral nerves.
Abstract: As a prelude to studies on retrograde axonal transport of neurotoxin (ie, so-called suicide transport) as a means to prevent post neurectomy neuroma formation, preliminary studies were conducted with an innocuous enzymatic marker, horseradish peroxidase (HRP). The proximal stumps of resected medial and lateral palmar digital nerves in six ponies were injected via a tuberculin syringe and needle with 50 micron 1 of a 30 per cent solution of HRP in order to assess long distance retrograde axonal transport. The dorsal root ganglion of the cervical spinal enlargement (ie, C6, C7, C8, T1, T2) were removed at post injection intervals of two, four, six, eight, 10 and 12 days. These were sectioned serially and reacted by the tetramethylbenzidine method to demonstrate transported enzyme in the ganglionic cell bodies which give rise to sensory fibres of the palmar digital nerves. Enzyme, retrogradely transported over axon lengths of 115 cm, was first demonstrated in spinal ganglia four days after injections of the palmar digital nerves. The calculated transport velocity of 287 mm/day, although almost certainly an underestimate, greatly exceeded rates of 72 to 120 mm/day recorded previously with HRP in the peripheral nerves of small laboratory animals. The intensity of the HRP reaction product in ganglionic neurons was strong at four days and it remained unabated in ganglia examined at six, eight, 10 and 12 days post injection. The major sources of the sensory fibres of the palmar digital nerves appeared to be the ganglia of the C8 and T1 spinal segments which contained more than 90 per cent of all labelled neurons.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1985-11-01 PubMed ID: 4076160DOI: 10.1111/j.2042-3306.1985.tb02554.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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This research was a preliminary study on the long-distance transfer of horseradish peroxidase (HRP) in horse peripheral nerves as an initial step towards studying neuron destruction as a means to prevent nerve tumor formation post-surgical removal.
Overview of the Study
- The study involved injecting a 30% solution of horseradish peroxidase into the stumps of resected medial and lateral palmar digital nerves of six ponies. HRP is an enzyme that can be detected and tracked to gain an understanding of the movement along the nerves.
- The objective was to study the phenomenon of retrograde axonal transport, a cellular process in which neurons transport molecules from the nerve terminal towards the cell body.
- After the injection, the dorsal root ganglion (clumps of nerve cell bodies) in the cervical spinal enlargement were removed at various intervals ranging from two to twelve days.
Results Summary
- The HRP enzyme was first detected in the spinal ganglia four days after injections in the palmar digital nerves. This indicates that the enzyme was transported over axon lengths of 115 cm.
- The calculated transport velocity of the enzyme was approximately 287 mm/day. This is a higher rate than previous studies with HRP (72-120 mm/day) in small laboratory animals’ peripheral nerves, suggesting that the neuron transport system varies between species or size of organisms.
- The intensity of the HRP enzyme was strong at four days and remained stable in ganglia tested at six, eight, ten, and twelve days post injection. This suggests the enzyme could be steadily and reliably tracked over time.
- Most of the sensory nerve fibers for the palmar digital nerves seemed to come from the C8 and T1 spinal segments, which contained over 90% of all labeled neurons.
Research Implications
- The results of this preliminary study pave the way for future research into using neurotoxin for “suicide transport” as a way to prevent the formation of neuromas (nerve tumors) after neurectomy (nerve removal surgery).
- Additionally, the study also provides valuable insights into understanding the neuronal cell transport system in larger animals.
Cite This Article
APA
Fubini SL, Cummings JF, Todhunter RJ.
(1985).
Preliminary studies on long distance, retrograde transport of horseradish peroxidase in equine peripheral nerves.
Equine Vet J, 17(6), 453-458.
https://doi.org/10.1111/j.2042-3306.1985.tb02554.x Publication
Researcher Affiliations
MeSH Terms
- Animals
- Axons / metabolism
- Biological Transport
- Female
- Ganglia, Spinal / metabolism
- Horseradish Peroxidase / metabolism
- Horses
- Male
- Neurons / metabolism
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