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Home / Equine Research Bank / Front Vet Sci / Presence of Myeloperoxidase in Lamellar Tissue of Horses Ind...
Frontiers in veterinary science2022; 9; 846835; doi: 10.3389/fvets.2022.846835

Presence of Myeloperoxidase in Lamellar Tissue of Horses Induced by an Euglycemic Hyperinsulinemic Clamp.

Abstract: Laminitis is a pathology of the equine digit leading to a failure of the dermo-epidermal interface. Neutrophil activation is recognized as a major factor in SIRS-associated laminitis. Less is known about the role of neutrophil activation in laminitis associated with metabolic disorders. The aim of this descriptive study was to observe whether myeloperoxidase is increased in the laminae during early stage laminitis in three horses subjected to a prolonged euglycemic hyperinsulinemic clamp (pEHC). After 48 h of pEHC-treatment, horses were subjected to euthanasia. Two healthy horses are used as control. Histological sections of lamellar tissue from all horses were immunohistochemically stained for myeloperoxidase and counterstained with hematoxylin-eosin. Histopathological changes that characterize insulin-induced laminitis and increased presence of myeloperoxidase, especially in the dermal lamellae, were increased in histologic sections of pEHC-treated horses. Neutrophil myeloperoxidase release may contribute to the pathophysiology of endocrinopathic laminitis.
Copyright © 2022 Storms, Medina Torres, Franck, Sole Guitart, de la Rebière and Serteyn.
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Publication Date: 2022-03-11 PubMed ID: 35359667PubMed Central: PMC8962398DOI: 10.3389/fvets.2022.846835Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the role of a substance called myeloperoxidase in a condition affecting horses’ hooves, known as laminitis. It observes an increase of myeloperoxidase during the early stage of laminitis when horses were subjected to a prolonged insulin-induced condition.

Objective of the Research

  • The study primarily aims to examine if there is an increase of myeloperoxidase, an enzyme stored in a type of white blood cell called neutrophil, in the laminae during the early stage of laminitis – a painful disease in horses that affects hoof tissues. This study was necessitated due to limited data related to neutrophil activation in laminitis linked to metabolic disorders.

Methodology

  • Three horses were subjected to a prolonged euglycemic hyperinsulinemic clamp (pEHC), a method that maintains blood sugar levels while increasing insulin. This was done to induce an insulin-related metabolic condition linked to laminitis in horses.
  • After 48 hours of this treatment, the horses were euthanized, and the lamellar tissue or the internal tissues of the horses’ hooves were harvested for testing.
  • These samples were then immunohistochemically stained to identify the presence of myeloperoxidase. This type of staining involves specific antibodies that can bind only to the enzyme of interest, thereby highlighting where it is located within the tissue.
  • The tissues were also counterstained with hematoxylin and eosin, a process that provides contrast to the tissue samples, enabling inspection of tissue structure and potential changes.
  • Two healthy horses were also subjected to similar procedure and used as control in the study.

Findings

  • Through histological inspection or microscopic examination of the hoof tissues, certain pathological changes that are considered traits of insulin-induced laminitis were identified.
  • An increased presence of myeloperoxidase, specifically in the dermal lamellae (a particular part of the tissue within the hoof), confirms the involvement of myeloperoxidase in horses subjected to pEHC.

Conclusion

  • The research, thus, concludes that the release of myeloperoxidase by neutrophils may potentially contribute to the pathophysiology of endocrinopathic laminitis, implying that it could play a role in the development of this painful hoof condition. This understanding lays the foundation for further studies around therapeutic strategies involving the management of myeloperoxidase levels in horses suffering from laminitis.

Cite This Article

APA
Storms N, Medina Torres C, Franck T, Sole Guitart A, de la Rebière G, Serteyn D. (2022). Presence of Myeloperoxidase in Lamellar Tissue of Horses Induced by an Euglycemic Hyperinsulinemic Clamp. Front Vet Sci, 9, 846835. https://doi.org/10.3389/fvets.2022.846835

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 9
Pages: 846835
PII: 846835

Researcher Affiliations

Storms, Nazare
  • Department of Equine Surgery, University of Liège, Liège, Belgium.
Medina Torres, Carlos
  • School of Veterinary Science, The University of Queensland, Gatton, QLD, Australia.
Franck, Thierry
  • Center for Oxygen Research and Development, FARAH, University of Liège, Liège, Belgium.
Sole Guitart, Albert
  • School of Veterinary Science, The University of Queensland, Gatton, QLD, Australia.
de la Rebière, Geoffroy
  • Department of Equine Surgery, University of Liège, Liège, Belgium.
Serteyn, Didier
  • Department of Equine Surgery, University of Liège, Liège, Belgium.
  • Center for Oxygen Research and Development, FARAH, University of Liège, Liège, Belgium.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Citations

This article has been cited 3 times.
  1. Parrilla Hernández S, Franck T, Munaut C, Feyereisen É, Piret J, Farnir F, Reigner F, Barrière P, Deleuze S. Characterization of Myeloperoxidase in the Healthy Equine Endometrium. Animals (Basel) 2023 Jan 21;13(3).
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  2. Sheahan BJ, Schubert AG, Schubert W, Sheats MK, Schnabel LV, Gilbertie JM. Equine neutrophils selectively release neutrophil extracellular traps in response to chemical and bacterial agonists. Front Vet Sci 2025;12:1512343.
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  3. Duysens J, Graide H, Niesten A, Mouithys-Mickalad A, Deby-Dupont G, Franck T, Ceusters J, Serteyn D. Culture and Immunomodulation of Equine Muscle-Derived Mesenchymal Stromal Cells: A Comparative Study of Innovative 2D versus 3D Models Using Equine Platelet Lysate. Cells 2024 Jul 31;13(15).
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