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Home / Equine Research Bank / J Virol / RNA-dependent DNA polymerase associated with equine infectio...
Journal of virology1977; 22(1); 16-22; doi: 10.1128/JVI.22.1.16-22.1977

RNA-dependent DNA polymerase associated with equine infectious anemia virus.

Abstract: Equine infectious anemia (EIAV) is shown to have an associated RNA-instructed DNA polymerase similar in its cofactor requirements and reaction conditions to the RNA tumor virus DNA polymerases. Demonstrating this DNA polymerase activity requires a critical concentration of a nonionic detergent, all four deoxyribonucleoside triphosphates, and a divalent metal ion. The reaction is sensitive to RNase, and a substantial fraction of the FNA synthesized is complementary to viral RNA. The detection of a complex of tritium-labeled polymerase product DNA-template RNA, which sedimented at 60S to 70S, provided evidence that EIAV contains high-molecular-weight RNA. These results, obtained with both virus propagated in cell culture and virus from the serum of an experimentally infected horse, indicate that EIAV may properly be considered a member of the family Retroviridae. They may also be pertinent to the mechanism(s) of viral persistence and periodic recrudescence of disease in chronically infected horses.
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Publication Date: 1977-04-01 PubMed ID: 67219PubMed Central: PMC515681DOI: 10.1128/JVI.22.1.16-22.1977Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the existence of an RNA-directed DNA polymerase associated with equine infectious anemia virus (EIAV), indicating that the EIAV might belong to the family Retroviridae. The research also discusses how this could be important for understanding viral persistence and recurring disease in horses with chronic infections.

Research Summary

  • The presented research aims to associate equine infectious anemia virus (EIAV) to RNA-directed DNA polymerase. This polymerase is crucial in the life cycle of retroviruses, allowing RNA to be converted into DNA for integration into a host’s genome.
  • The research has identified similar cofactor requirements and reaction conditions between this RNA tumor virus DNA polymerase and the one associated with EIAV, such as the need for a specific concentration of a nonionic detergent, all four deoxyribonucleoside triphosphates, and a divalent metal ion.

Methodology

  • The study used both lab-cultured virus as well as virus obtained from an experimentally infected horse’s serum to investigate RNA-dependent DNA polymerase. The reaction was measured to be sensitive to RNases, indicating the incorporation of viral RNA.
  • Steps were also taken to demonstrate the creation of DNA that is complementary to viral RNA.

Results and Conclusion

  • A complex of tritium-labeled polymerase product DNA-template RNA was discovered, sedimenting at 60S to 70S. This served as evidence for EIAV containing high-molecular-weight RNA.
  • From these findings, the study concludes that EIAV could be classified as a member of the Retroviridae family.
  • The results could potentially shed light on the mechanisms responsible for viral persistence and recurring disease in chronically infected horses.

Cite This Article

APA
Archer BG, Crawford TB, McGuire TC, Frazier ME. (1977). RNA-dependent DNA polymerase associated with equine infectious anemia virus. J Virol, 22(1), 16-22. https://doi.org/10.1128/JVI.22.1.16-22.1977

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 22
Issue: 1
Pages: 16-22

Researcher Affiliations

Archer, B G
    Crawford, T B
      McGuire, T C
        Frazier, M E

          MeSH Terms

          • DNA, Viral / biosynthesis
          • Dactinomycin / pharmacology
          • Infectious Anemia Virus, Equine / analysis
          • Infectious Anemia Virus, Equine / enzymology
          • Infectious Anemia Virus, Equine / metabolism
          • Magnesium / metabolism
          • Manganese / metabolism
          • Nucleic Acid Hybridization
          • Nucleotides / metabolism
          • RNA, Viral / analysis
          • RNA, Viral / metabolism
          • RNA-Directed DNA Polymerase / metabolism
          • Ribonucleases / metabolism
          • Surface-Active Agents / pharmacology
          • Templates, Genetic

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