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Home / Equine Research Bank / Mem Inst Oswaldo Cruz / Serologic survey of West Nile virus in horses from Central-W...
Memorias do Instituto Oswaldo Cruz2013; 108(7); 921-923; doi: 10.1590/S0074-02762013005000001

Serologic survey of West Nile virus in horses from Central-West, Northeast and Southeast Brazil.

Abstract: Since the emergence of West Nile virus (WNV) in North America in 1999, there have been several reports of WNV activity in Central and South American countries. To detect WNV in Brazil, we performed a serological survey of horses from different regions of Brazil using recombinant peptides from domain III of WNV. Positive samples were validated with the neutralisation test. Our results showed that of 79 ELISA-positive horses, nine expressed WNV-specific neutralising antibodies. Eight of the infected horses were from the state of Mato Grosso do Sul and one was from the state of Paraíba. Our results provide additional evidence for the emergence of WNV in Brazil and for its circulation in multiple regions of the country.
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Publication Date: 2013-09-17 PubMed ID: 24037110PubMed Central: PMC3970643DOI: 10.1590/S0074-02762013005000001Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the presence of West Nile virus (WNV) in various regions of Brazil by testing horses for the virus with recombinant peptides. Out of 79 horses that tested positive via ELISA, further neutralisation tests confirmed nine expressed WNV-specific neutralising antibodies, indicating the virus’s presence in Brazil.

Study Methodology

  • The researchers carried out a serological (antibody) survey on horses from different regions of Brazil to detect the presence of West Nile virus.
  • They utilised recombinant peptides from the domain III of the virus for the survey. Recombinant peptides are synthetic proteins that mimic the properties of natural ones and are often used in the medical field for diagnosing disease.
  • Those horses that tested positive in the ELISA (Enzyme-Linked Immunosorbent Assay – a common antibody test) then underwent a neutralisation test to confirm the findings. A neutralisation test checks for the presence of specific antibodies that can neutralise a virus, in this case, the WNV.

Study Findings

  • The study found that out of 79 ELISA-positive horses, nine expressed WNV-specific neutralising antibodies, confirming the presence of the virus.
  • The majority of the infected horses (eight) were from the state of Mato Grosso do Sul, with one horse from the state of Paraíba also testing positive.

Implications of the Research

  • The results of this study provide further evidence for the emergence of the West Nile virus in Brazil, indicating its presence and circulation within different regions of the country.
  • The findings highlight the need for increased surveillance of WNV in horses, as they serve as key indicators for the presence of the virus, due to their susceptibility and geographical distribution.
  • Further research is required to determine the extent of WNV infection in other animals and possible vectors, to proactively manage and prevent the spread of this virus within Brazil.

Cite This Article

APA
Silva JR, Medeiros LC, Reis VP, Chavez JH, Munhoz TD, Borges GP, Soares OA, Campos CH, Machado RZ, Baldani CD, Silva ML, Faria JL, Silva EE, Figueiredo LT. (2013). Serologic survey of West Nile virus in horses from Central-West, Northeast and Southeast Brazil. Mem Inst Oswaldo Cruz, 108(7), 921-923. https://doi.org/10.1590/S0074-02762013005000001

Publication

Memorias do Instituto Oswaldo Cruz
ISSN: 1678-8060
NlmUniqueID: 7502619
Country: Brazil
Language: English
Volume: 108
Issue: 7
Pages: 921-923

Researcher Affiliations

Silva, Jaqueline Raymondi
    Medeiros, Larissa Campos de
      Reis, Vinicius Pinho dos
        Chavez, Juliana Helena
          Munhoz, Thiago Demarchi
            Borges, Gustavo Puia
              Soares, Otavio Augusto Brioschi
                Campos, Carlos Henrique Coelho de
                  Machado, Rosangela Zacarias
                    Baldani, Cristiane Divan
                      Silva, Maria Luana Cristiny Rodrigues
                        Faria, Joice Lara Maia
                          Silva, Edson Elias da
                            Figueiredo, Luiz Tadeu Moraes

                              MeSH Terms

                              • Animals
                              • Antibodies, Viral / blood
                              • Brazil / epidemiology
                              • Enzyme-Linked Immunosorbent Assay / veterinary
                              • Horse Diseases / diagnosis
                              • Horse Diseases / epidemiology
                              • Horse Diseases / virology
                              • Horses
                              • Neutralization Tests
                              • Seroepidemiologic Studies
                              • West Nile Fever / diagnosis
                              • West Nile Fever / epidemiology
                              • West Nile Fever / veterinary
                              • West Nile virus / immunology
                              • West Nile virus / isolation & purification

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