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Veterinary journal (London, England : 1997)2017; 220; 91-94; doi: 10.1016/j.tvjl.2017.01.007

Seroprevalence and risk factors for infection with equine coronavirus in healthy horses in the USA.

Abstract: Equine coronavirus (ECoV) is considered an enteric pathogen of foals and has only recently been associated with infections in adult horses. Seroprevalence data is needed to better understand the epidemiology of ECoV in adult horses, evaluate diagnostic modalities and develop preventive measures. The objective of this study was to investigate the seroprevalence and selective risk factors for ECoV in 5247 healthy adult horses in the USA, using a recently established and validated IgG enzyme-linked immunosorbent assay. Prevalence factors analysed in this study included geographic region, age, breed, sex and use. A total of 504/5247 horses (9.6%) horses tested seropositive. Geographic region (Mid-West; P = 0.008), breed (Draft horses; P = 0.003) and specific uses of horses (ranch/farm, P = 0.034; breeding use, P = 0.016) were all statistically significant risk factors for seropositivity.
Copyright © 2017. Published by Elsevier Ltd.
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Publication Date: 2017-01-12 PubMed ID: 28190504PubMed Central: PMC7110631DOI: 10.1016/j.tvjl.2017.01.007Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research looks into the occurrence and risk factors associated with equine coronavirus, a typically foal-affecting disease, in healthy adult horses in the USA. The findings point out certain factors such as geographical location, breed, and specific usage of the horses that influence the likelihood of seropositivity.

Objective of the Study

  • The main goal of this research was to investigate the prevalence of Equine Coronavirus (ECoV) and distinct risk factors in over 5000 healthy adult horses in the USA. To achieve this, researchers used a recently created and validated testing method – the IgG enzyme-linked immunosorbent assay.

Reasons for the Study

  • ECoV is generally known as an enteric pathogen that primarily affects foals. It has however been recently linked to infections in adult horses, which necessitates the gathering of seroprevalence data.
  • Seroprevalence data is required to aid in better understanding the epidemiology of ECoV in adult horses, evaluating diagnostic procedures and formulating preventive measures.

Methodology

  • In this research, the severity or the frequency of the ECoV in adult horses was analyzed based on different factors such as geographic location, age, breed, sex, and use of the horses.
  • A total of 5247 horses were tested and among them, 504 horses (9.6%) tested positive for the ECoV antibodies. This shows their past or ongoing infection with the virus.

Results and Findings

  • The study found that the geographical region, specifically the Mid-West, was a significant risk factor for ECoV with a significance level of P = 0.008.
  • Breed was another determining factor. Particularly, Draft horses were associated with a higher risk (P = 0.003).
  • The specific uses of horses were also found to be statistical risk factors for ECoV. Horses used for ranch/farm purposes had a significance level of P = 0.034, while those used for breeding purposes had a P value of 0.016.

Conclusion

  • This study contributes to a better understanding of the epidemiology of ECoV in adult horses in the USA. The discovery of risk factors such as geographic location, breed, and specific uses of horses can aid in evaluating the diagnostic modalities of ECoV and in the formulation of effective preventive measures.

Cite This Article

APA
Kooijman LJ, James K, Mapes SM, Theelen MJ, Pusterla N. (2017). Seroprevalence and risk factors for infection with equine coronavirus in healthy horses in the USA. Vet J, 220, 91-94. https://doi.org/10.1016/j.tvjl.2017.01.007

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 220
Pages: 91-94
PII: S1090-0233(17)30021-7

Researcher Affiliations

Kooijman, L J
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 114, Utrecht 3584CM, The Netherlands.
James, K
  • Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, California 95616, USA.
Mapes, S M
  • Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, California 95616, USA.
Theelen, M J P
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 114, Utrecht 3584CM, The Netherlands.
Pusterla, N
  • Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, California 95616, USA. Electronic address: npusterla@ucdavis.edu.

MeSH Terms

  • Animals
  • Coronavirus / physiology
  • Coronavirus Infections / epidemiology
  • Coronavirus Infections / veterinary
  • Coronavirus Infections / virology
  • Coronavirus, Bovine
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Female
  • Horse Diseases / epidemiology
  • Horse Diseases / virology
  • Horses
  • Male
  • Risk Factors
  • Seroepidemiologic Studies
  • United States / epidemiology

References

This article includes 15 references
  1. Chan KH, Peiris JS, Lam SY, Poon LL, Yuen KY, Seto WH. The Effects of Temperature and Relative Humidity on the Viability of the SARS Coronavirus.. Adv Virol 2011;2011:734690.
    pmc: PMC3265313pubmed: 22312351doi: 10.1155/2011/734690google scholar: lookup
  2. Fielding CL, Higgins JK, Higgins JC, McIntosh S, Scott E, Giannitti F, Mete A, Pusterla N. Disease associated with equine coronavirus infection and high case fatality rate.. J Vet Intern Med 2015 Jan;29(1):307-10.
    pmc: PMC4858071pubmed: 25319406doi: 10.1111/jvim.12480google scholar: lookup
  3. Giannitti F, Diab S, Mete A, Stanton JB, Fielding L, Crossley B, Sverlow K, Fish S, Mapes S, Scott L, Pusterla N. Necrotizing Enteritis and Hyperammonemic Encephalopathy Associated With Equine Coronavirus Infection in Equids.. Vet Pathol 2015 Nov;52(6):1148-56.
    pubmed: 25648965doi: 10.1177/0300985814568683google scholar: lookup
  4. Guy JS, Breslin JJ, Breuhaus B, Vivrette S, Smith LG. Characterization of a coronavirus isolated from a diarrheic foal.. J Clin Microbiol 2000 Dec;38(12):4523-6.
    pmc: PMC87631pubmed: 11101590doi: 10.1128/JCM.38.12.4523-4526.2000google scholar: lookup
  5. Kooijman LJ, Mapes SM, Pusterla N. Development of an equine coronavirus-specific enzyme-linked immunosorbent assay to determine serologic responses in naturally infected horses.. J Vet Diagn Invest 2016 Jul;28(4):414-8.
    pubmed: 27216723doi: 10.1177/1040638716649643google scholar: lookup
  6. Miszczak F, Tesson V, Kin N, Dina J, Balasuriya UB, Pronost S, Vabret A. First detection of equine coronavirus (ECoV) in Europe.. Vet Microbiol 2014 Jun 25;171(1-2):206-9.
    pmc: PMC7117151pubmed: 24768449doi: 10.1016/j.vetmic.2014.03.031google scholar: lookup
  7. Ohlson A, Heuer C, Lockhart C, Tråvén M, Emanuelson U, Alenius S. Risk factors for seropositivity to bovine coronavirus and bovine respiratory syncytial virus in dairy herds.. Vet Rec 2010 Aug 7;167(6):201-6.
    pubmed: 20693503doi: 10.1136/vr.c4119google scholar: lookup
  8. Oue Y, Ishihara R, Edamatsu H, Morita Y, Yoshida M, Yoshima M, Hatama S, Murakami K, Kanno T. Isolation of an equine coronavirus from adult horses with pyrogenic and enteric disease and its antigenic and genomic characterization in comparison with the NC99 strain.. Vet Microbiol 2011 May 12;150(1-2):41-8.
    pmc: PMC7117184pubmed: 21273011doi: 10.1016/j.vetmic.2011.01.004google scholar: lookup
  9. Oue Y, Morita Y, Kondo T, Nemoto M. Epidemic of equine coronavirus at Obihiro Racecourse, Hokkaido, Japan in 2012.. J Vet Med Sci 2013;75(9):1261-5.
    pubmed: 23648375doi: 10.1292/jvms.13-0056google scholar: lookup
  10. Pusterla N, Mapes S, Wademan C, White A, Ball R, Sapp K, Burns P, Ormond C, Butterworth K, Bartol J, Magdesian KG. Emerging outbreaks associated with equine coronavirus in adult horses.. Vet Microbiol 2013 Feb 22;162(1):228-31.
    pmc: PMC7117461pubmed: 23123176doi: 10.1016/j.vetmic.2012.10.014google scholar: lookup
  11. Slovis NM, Elam J, Estrada M, Leutenegger CM. Infectious agents associated with diarrhoea in neonatal foals in central Kentucky: a comprehensive molecular study.. Equine Vet J 2014 May;46(3):311-6.
    pmc: PMC7163618pubmed: 23773143doi: 10.1111/evj.12119google scholar: lookup
  12. Tråvén M, Näslund K, Linde N, Linde B, Silván A, Fossum C, Hedlund KO, Larsson B. Experimental reproduction of winter dysentery in lactating cows using BCV -- comparison with BCV infection in milk-fed calves.. Vet Microbiol 2001 Jul 26;81(2):127-51.
    pmc: PMC7117383pubmed: 11376958doi: 10.1016/s0378-1135(01)00337-6google scholar: lookup
  13. Woo PC, Lau SK, Huang Y, Yuen KY. Coronavirus diversity, phylogeny and interspecies jumping.. Exp Biol Med (Maywood) 2009 Oct;234(10):1117-27.
    pubmed: 19546349doi: 10.3181/0903-MR-94google scholar: lookup
  14. Woo PC, Lau SK, Lam CS, Lau CC, Tsang AK, Lau JH, Bai R, Teng JL, Tsang CC, Wang M, Zheng BJ, Chan KH, Yuen KY. Discovery of seven novel Mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus.. J Virol 2012 Apr;86(7):3995-4008.
    pmc: PMC3302495pubmed: 22278237doi: 10.1128/JVI.06540-11google scholar: lookup
  15. Zhang J, Guy JS, Snijder EJ, Denniston DA, Timoney PJ, Balasuriya UB. Genomic characterization of equine coronavirus.. Virology 2007 Dec 5;369(1):92-104.
    pmc: PMC7103287pubmed: 17706262doi: 10.1016/j.virol.2007.06.035google scholar: lookup

Citations

This article has been cited 21 times.
  1. Liguori G, Costagliola A, Lombardi R, Paciello O, Giordano A. Human-Animal Interaction in Animal-Assisted Interventions (AAI)s: Zoonosis Risks, Benefits, and Future Directions-A One Health Approach. Animals (Basel) 2023 May 9;13(10).
    doi: 10.3390/ani13101592pubmed: 37238022google scholar: lookup
  2. Bannai H, Kambayashi Y, Ohta M, Nemoto M, Tsujimura K. Prevalence of serum and salivary virus-neutralizing antibodies against equine coronavirus in four riding stables in Japan. J Equine Sci 2023 Mar;34(1):13-18.
    doi: 10.1294/jes.34.13pubmed: 37155492google scholar: lookup
  3. Hepworth-Warren KL, Erwin SJ, Moore CB, Talbot JR, Young KAS, Neault MJ, Haugland JC, Robertson JB, Blikslager AT. Risk factors associated with an outbreak of equine coronavirus at a large farm in North Carolina. Front Vet Sci 2023;10:1060759.
    doi: 10.3389/fvets.2023.1060759pubmed: 36937023google scholar: lookup
  4. Shehata AA, Attia YA, Rahman MT, Basiouni S, El-Seedi HR, Azhar EI, Khafaga AF, Hafez HM. Diversity of Coronaviruses with Particular Attention to the Interspecies Transmission of SARS-CoV-2. Animals (Basel) 2022 Feb 4;12(3).
    doi: 10.3390/ani12030378pubmed: 35158701google scholar: lookup
  5. Uzal FA, Arroyo LG, Navarro MA, Gomez DE, Asín J, Henderson E. Bacterial and viral enterocolitis in horses: a review. J Vet Diagn Invest 2022 May;34(3):354-375.
    doi: 10.1177/10406387211057469pubmed: 34763560google scholar: lookup
  6. Schvartz G, Tirosh-Levy S, Barnum S, David D, Sol A, Pusterla N, Steinman A. Seroprevalence and Risk Factors for Exposure to Equine Coronavirus in Apparently Healthy Horses in Israel. Animals (Basel) 2021 Mar 21;11(3).
    doi: 10.3390/ani11030894pubmed: 33800990google scholar: lookup
  7. Colina SE, Serena MS, Echeverría MG, Metz GE. Clinical and molecular aspects of veterinary coronaviruses. Virus Res 2021 May;297:198382.
    doi: 10.1016/j.virusres.2021.198382pubmed: 33705799google scholar: lookup
  8. Khamassi Khbou M, Daaloul Jedidi M, Bouaicha Zaafouri F, Benzarti M. Coronaviruses in farm animals: Epidemiology and public health implications. Vet Med Sci 2021 Mar;7(2):322-347.
    doi: 10.1002/vms3.359pubmed: 32976707google scholar: lookup
  9. Wright S. Highlights of recent clinically relevant papers. Equine Vet Educ 2017 Apr;29(4):178-179.
    doi: 10.1111/eve.12743pubmed: 32313393google scholar: lookup
  10. Mattei DN, Kopper JJ, Sanz MG. Equine Coronavirus-Associated Colitis in Horses: A Retrospective Study. J Equine Vet Sci 2020 Apr;87:102906.
    doi: 10.1016/j.jevs.2019.102906pubmed: 32172908google scholar: lookup
  11. Lönker NS, Fechner K, Wahed AAE. Horses as a Crucial Part of One Health. Vet Sci 2020 Feb 29;7(1).
    doi: 10.3390/vetsci7010028pubmed: 32121327google scholar: lookup
  12. Zhao S, Smits C, Schuurman N, Barnum S, Pusterla N, Kuppeveld FV, Bosch BJ, Maanen KV, Egberink H. Development and Validation of a S1 Protein-Based ELISA for the Specific Detection of Antibodies against Equine Coronavirus. Viruses 2019 Nov 30;11(12).
    doi: 10.3390/v11121109pubmed: 31801275google scholar: lookup
  13. Nemoto M, Schofield W, Cullinane A. The First Detection of Equine Coronavirus in Adult Horses and Foals in Ireland. Viruses 2019 Oct 14;11(10).
    doi: 10.3390/v11100946pubmed: 31615132google scholar: lookup
  14. Berryhill EH, Magdesian KG, Aleman M, Pusterla N. Clinical presentation, diagnostic findings, and outcome of adult horses with equine coronavirus infection at a veterinary teaching hospital: 33 cases (2012-2018). Vet J 2019 Jun;248:95-100.
    doi: 10.1016/j.tvjl.2019.05.001pubmed: 31113572google scholar: lookup
  15. Sanz MG, Kwon S, Pusterla N, Gold JR, Bain F, Evermann J. Evaluation of equine coronavirus fecal shedding among hospitalized horses. J Vet Intern Med 2019 Mar;33(2):918-922.
    doi: 10.1111/jvim.15449pubmed: 30788861google scholar: lookup
  16. Pusterla N, Vin R, Leutenegger CM, Mittel LD, Divers TJ. Enteric coronavirus infection in adult horses. Vet J 2018 Jan;231:13-18.
    doi: 10.1016/j.tvjl.2017.11.004pubmed: 29429482google scholar: lookup
  17. Kouadria W, Poder SL, van Maanen K, Seuberlich T, Dawson KLD, Zientara S, Laabassi F. First serological evidence of equine coronavirus and severe acute respiratory syndrome coronavirus 2 in horses in North Africa. Vet Res Commun 2025 Oct 4;49(6):347.
    doi: 10.1007/s11259-025-10928-0pubmed: 41045398google scholar: lookup
  18. Ricci I, Rosone F, Pacchiarotti G, Manna G, Cersini A, Carvelli A, La Rocca D, Cammalleri E, Giordani R, Tofani S, Conti R, Rombolà P, Nardini R, Minniti CA, Caforio R, Linardi B, Scicluna MT. Pegiviruses and Coronavirus: Biomolecular Prevalence and Phylogenetic Analysis of Strains Detected in Italian Horse Populations. Viruses 2025 Aug 2;17(8).
    doi: 10.3390/v17081076pubmed: 40872790google scholar: lookup
  19. Sun Y, Yu YT, Castillo XO, Anderson R, Wang M, Sun Q, Tallmadge R, Sams K, Reboul G, Zehr J, Brown J, Wang X, Marra N, Stanhope B, Grenier J, Pusterla N, Divers T, Mittel L, Goodman LB. Investigation of the Blood Microbiome in Horses With Fever of Unknown Origin. Vet Med Sci 2025 Mar;11(2):e70272.
    doi: 10.1002/vms3.70272pubmed: 40065594google scholar: lookup
  20. Haywood LMB, Sheahan BJ. A Review of Epithelial Ion Transporters and Their Roles in Equine Infectious Colitis. Vet Sci 2024 Oct 7;11(10).
    doi: 10.3390/vetsci11100480pubmed: 39453072google scholar: lookup
  21. Pusterla N, Lawton K, Barnum S. Investigation of the seroprevalence to equine coronavirus and SARS-CoV-2 in healthy adult horses recently imported to the United States. Vet Q 2024 Dec;44(1):1-6.
    doi: 10.1080/01652176.2023.2288876pubmed: 38010292google scholar: lookup
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