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The Canadian veterinary journal = La revue veterinaire canadienne2015; 56(8); 831-838;

Short- and long-term effects of platelet-rich plasma upon healthy equine joints: Clinical and laboratory aspects.

Abstract: This study aimed to verify whether transient inflammatory reactions incited by the administration of intra-articular platelet-rich plasma (PRP) affected joint components through short- and long-term in vivo evaluation of inflammatory biomarkers and extracellular matrix degradation products in synovial fluid. The effects of PRP were analyzed in a short phase protocol (SPP) and in a prolonged phase protocol (PPP), using saline-injected joints as controls. In the SPP, higher white blood cell counts and prostaglandin E2 and total protein concentrations were observed in the synovial fluid of PRP-treated joints (P < 0.05). There were no differences between the interleukin-1β, interleukin-1 receptor antagonist protein, tumor necrosis factor-α, chondroitin sulfate, or hyaluronic acid concentrations between PRP and saline injected joints. In the PPP, there were no differences in evaluated parameters between groups. PRP injection elicits a mild and self-limiting inflammatory response shortly after administration, without long-term deleterious effects on joint homeostasis. Effets à court et à long terme de le plasma riche en plaquettes sur les articulations sains chez les équines : aspects cliniques et laboratoiren. Cette étude a pour but de vérifier si les réactions inflammatoires passagères induites par l’administration intra articulaire de Plasma Enrichi en Plaquettes (PRP) affectent les composants articulaires. Les bio-marqueurs de l’inflammation et les produits de dégradation de la matrice extracellulaire ont été évalués in vivo, dans le liquide synovial, à court et long terme. Les effets du PRP ont été analysés lors d’un protocole court terme et lors d’un protocole long terme et les articulations contrôles ont été injectées avec du liquide physiologique. Le protocole court terme a révélé des comptages de globules blancs et de prostaglandine E2, ainsi que des concentrations en protéines totales plus élevés dans le liquide synovial des articulations traitées au PRP (P < 0,05). Cependant, aucune différence de concentration en interleukine-1 bêta, en protéine antagoniste des récepteurs à l’interleukine-1, en facteur de nécrose tumorale alpha, en chondroïtine sulfate et en acide hyaluronique n’a été notée entre les articulations injectées au PRP et les articulations contrôles. Le protocole long terme n’a démontré aucune différence des paramètres évalués entre les deux groupes. L’injection de PRP provoque une réponse inflammatoire légère et auto-limitante rapidement après l’administration, sans effet délétère sur l’homéostasie de l’articulation à long terme.(Traduit par les auteurs).
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Publication Date: 2015-08-08 PubMed ID: 26246629PubMed Central: PMC4502851
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  • Clinical Trial
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on the impact of intra-articular platelet-rich plasma (PRP) injections on joint health in horses, both in the short-term and long-term. It revealed that while PRP injections initially cause a mild inflammatory response, there are no lasting negative effects on the joint.

Research Objective

  • The study aimed to investigate the short and long term impacts of platelet-rich plasma (PRP) injections on joint health, specifically focusing on potential inflammatory reactions and the impact on joint components.

Methodology

  • The effects of PRP were analyzed using two different protocols: a short phase protocol (SPP) and a prolonged phase protocol (PPP).
  • The SPP focused on the immediate after-effects of the PRP treatment, while the PPP analyzed longer-term impacts.
  • Saline-injected joints were used as controls for comparison purposes.

Results

  • In the SPP, the synovial fluid in PRP-treated joints showed elevated levels of white blood cells, prostaglandin E2, and total protein concentrations.
  • However, there were no significant differences in interleukin-1β, interleukin-1 receptor antagonist protein, tumor necrosis factor-α, chondroitin sulfate, and hyaluronic acid levels between PRP-treated joints and the control group.
  • In the PPP, no differences were observed in the studied parameters between PRP-treated joints and the control group.

Conclusion

  • PRP injections cause a mild and self-limiting inflammatory response in the short term.
  • Importantly, no long-term harmful effects on joint health were observed after the PRP treatment.

These findings suggest that the therapeutic use of PRP injections for joint treatment can be considered safe, pertaining to its short term mild inflammatory response and absence of adverse long-term effects.

Cite This Article

APA
Moraes AP, Moreira JJ, Brossi PM, Machado TS, Michelacci YM, Baccarin RY. (2015). Short- and long-term effects of platelet-rich plasma upon healthy equine joints: Clinical and laboratory aspects. Can Vet J, 56(8), 831-838.

Publication

The Canadian veterinary journal = La revue veterinaire canadienne
ISSN: 0008-5286
NlmUniqueID: 0004653
Country: Canada
Language: English
Volume: 56
Issue: 8
Pages: 831-838

Researcher Affiliations

Moraes, Ana Paula L
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
Moreira, Juliana J
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
Brossi, Patrícia M
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
Machado, Thaís S L
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
Michelacci, Yara M
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.
Baccarin, Raquel Y A
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia (Moraes, Moreira, Brossi, Machado, Baccarin), Departamento de Bioquímica (Michelacci), Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cep 05508 270, São Paulo SP, Brasil.

MeSH Terms

  • Animals
  • Biomarkers / metabolism
  • Cytokines / chemistry
  • Cytokines / genetics
  • Cytokines / metabolism
  • Female
  • Gene Expression Regulation / drug effects
  • Horses / physiology
  • Inflammation / metabolism
  • Inflammation / veterinary
  • Injections, Intra-Articular
  • Male
  • Platelet-Rich Plasma
  • Synovial Fluid / chemistry

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Citations

This article has been cited 13 times.
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