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Animals : an open access journal from MDPI2024; 14(9); doi: 10.3390/ani14091258

Single-Cell Transcriptome Sequencing Reveals Molecular Expression Differences and Marker Genes in Testes during the Sexual Maturation of Mongolian Horses.

Abstract: This study aimed to investigate differences in testicular tissue morphology, gene expression, and marker genes between sexually immature (1-year-old) and sexually mature (10-year-old) Mongolian horses. The purposes of our research were to provide insights into the reproductive physiology of male Mongolian horses and to identify potential markers for sexual maturity. The methods we applied included the transcriptomic profiling of testicular cells using single-cell sequencing techniques. Our results revealed significant differences in tissue morphology and gene expression patterns between the two age groups. Specifically, 25 cell clusters and 10 cell types were identified, including spermatogonial and somatic cells. Differential gene expression analysis highlighted distinct patterns related to cellular infrastructure in sexually immature horses and spermatogenesis in sexually mature horses. Marker genes specific to each stage were also identified, including APOA1, AMH, TAC3, INHA, SPARC, and SOX9 for the sexually immature stage, and PRM1, PRM2, LOC100051500, PRSS37, HMGB4, and H1-9 for the sexually mature stage. These findings contribute to a deeper understanding of testicular development and spermatogenesis in Mongolian horses and have potential applications in equine reproductive biology and breeding programs. In conclusion, this study provides valuable insights into the molecular mechanisms underlying sexual maturity in Mongolian horses.
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Publication Date: 2024-04-23 PubMed ID: 38731262PubMed Central: PMC11082968DOI: 10.3390/ani14091258Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers conducted a study to understand the differences in the testicular tissue structure and gene expression in sexually mature and immature Mongolian horses. Their ultimate goal was to learn more about the reproductive biology of these horses and pinpoint markers of sexual maturity.

Research Objectives

  • The main aim of this research was to differentiate the testicular tissue morphology, gene expression, and marker genes between sexually mature (10-year-old) and sexually immature (1-year-old) Mongolian horses.
  • The study aimed to gain a better understanding of the reproductive physiology of male Mongolian horses.
  • The researchers also sought to identify potential markers indicating sexual maturity in Mongolian horses.

Research Methodology

  • The research process involved transcriptomic profiling of testicular cells, a technique that was accomplished through single-cell sequencing.

Key Findings

  • Significant differences were observed in tissue morphology and gene expression patterns between the two age groups under study.
  • In particular, 25 cell clusters and 10 different cell types were identified, which included spermatogonial and somatic cells.
  • Differential gene expression analysis revealed distinct patterns related to cellular infrastructure in sexually immature horses and spermatogenesis (the process of male gamete formation) in sexually mature horses.
  • The research team was able to identify marker genes specific to each stage of maturation. For the sexually immature stage, these included six different specific genes. Similarly, six distinct genes were identified for the sexually mature stage.

Conclusion and Implications

  • Overall, the study added critical insights into the molecular mechanisms that underpin sexual maturity in Mongolian horses. This information might be valuable in the broader field of equine reproductive biology, potentially impacting breeding programs.
  • The findings allow for a deeper understanding of testicular development and spermatogenesis in these horses. The distinct genes identified at each stage of maturation could potentially serve as markers for sexual maturity.

Cite This Article

APA
Liu Y, Du M, Li X, Zhang L, Zhao B, Wang N, Dugarjaviin M. (2024). Single-Cell Transcriptome Sequencing Reveals Molecular Expression Differences and Marker Genes in Testes during the Sexual Maturation of Mongolian Horses. Animals (Basel), 14(9). https://doi.org/10.3390/ani14091258

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 9

Researcher Affiliations

Liu, Yuanyi
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Du, Ming
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Li, Xinyu
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhang, Lei
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhao, Bilig
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Wang, Na
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.
Dugarjaviin, Manglai
  • Key Laboratory of Equus Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Equus Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.

Grant Funding

  • QT202215 / High level Achievement Cultivation Project of College of Animal Science, Inner Mongolia Agricultural University

Conflict of Interest Statement

The authors declare no conflicts of interest.

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