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Home / Equine Research Bank / Stem Cell Rev Rep / SIRT1+ Adipose Derived Mesenchymal Stromal Stem Cells (ASCs)...
Stem cell reviews and reports2020; 16(6); 1328-1334; doi: 10.1007/s12015-020-10025-6

SIRT1+ Adipose Derived Mesenchymal Stromal Stem Cells (ASCs) Suspended in Alginate Hydrogel for the Treatment of Subchondral Bone Cyst in Medial Femoral Condyle in the Horse. Clinical Report.

Abstract: Stem cell based therapy are now commonly applied in human and veterinary medical practice especially in orthopaedics. Mesenchymal stromal stem cells isolated from adipose tissue (ASC) are first choice option due to relatively non-invasive and safe procedure of tissue harvesting. However, ASC therapeutic potential strongly rely on patients general health condition, age and life-style. For that reason, to enhance therapeutic potential of cells, they are modified in vitro using different approaches. Previous studies have shown, that ASC treated with resveratrol, herein called SIRT+, are characterised by decreased senescence, increased proliferation rate and improved clinical outcome in autologous therapies. Herein, SIRT + cells in alginate hydrogel were applied to 5 years old warm breed mare was clinically evaluated due to the left hind lameness due to subchondral bone cyst. The therapeutic effect was assessed by the analysis of lameness score and radiological evaluation. This case report demonstrates the therapeutic potential of SIRT + cells in the treatment of orthopaedics disorders in horses as complete bone remodelling occurred after therapy and horse came back to training.
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Publication Date: 2020-08-18 PubMed ID: 32803696PubMed Central: PMC7667135DOI: 10.1007/s12015-020-10025-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research reports on the successful use of a stem cell therapy technique, where stem cells are extracted from fat tissue, enhanced in vitro, and suspended in alginate hydrogel for the treatment of a subchondral bone cyst in a horse’s knee.

Study Overview:

  • Stem cell therapy is increasingly used in medical practice, especially in orthopedics. Among various types of stem cells, Mesenchymal Stromal Stem Cells (ASCs) derived from adipose (fat) tissue are preferred due to the relatively non-invasive and safe process of tissue harvesting.
  • Although the therapeutic efficacy of ASCs depends on factors such as a patient’s overall health, age, and lifestyle, these cells can be enhanced in vitro through various techniques.
  • The subject of this particular case report is a 5-year-old mare suffering from lameness due to a subchondral bone cyst in the left hind knee. The mare was treated with ASCs that had been enhanced with resveratrol (termed SIRT+ cells) and suspended in alginate hydrogel.

Findings:

  • Prior studies have demonstrated that ASCs treated with resveratrol (i.e., SIRT+ cells) exhibit reduced senescence (aging), an increased rate of proliferation, and improved clinical outcomes in autologous therapies (those using a patient’s own cells).
  • In this case report, the therapeutic effect of the treatment was assessed using a lameness score and radiological evaluation.
  • The results indicate that the treatment with SIRT+ cells in alginate hydrogel was successful: the horse showed complete bone remodeling following the therapy, and was able to return to training.

Implications:

  • This study reinforces the therapeutic potential of SIRT+ cells for treating orthopedic disorders in horses, highlighting the use of the alginate hydrogel as a delivery method.
  • The findings open a path for more in-depth exploration and applications of ASCs for healing bone lesions in horses and potentially other animals.
  • While this study is focused on veterinary medicine, the reported success of the stem cell therapy technique could be potentially influential in human orthopedic treatments as well.

Cite This Article

APA
Golonka P, Kornicka-Garbowska K, Marycz K. (2020). SIRT1+ Adipose Derived Mesenchymal Stromal Stem Cells (ASCs) Suspended in Alginate Hydrogel for the Treatment of Subchondral Bone Cyst in Medial Femoral Condyle in the Horse. Clinical Report. Stem Cell Rev Rep, 16(6), 1328-1334. https://doi.org/10.1007/s12015-020-10025-6

Publication

Stem cell reviews and reports
ISSN: 2629-3277
NlmUniqueID: 101752767
Country: United States
Language: English
Volume: 16
Issue: 6
Pages: 1328-1334

Researcher Affiliations

Golonka, Paweł
  • Equine Clinic Equivet, Gęsice 8, Domaniów, 55-216, Poland.
Kornicka-Garbowska, Katarzyna
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Norwida 27B Street, A7 building, Wroclaw, 50-375, Poland.
  • International Institute of Translational Medicine, Malin, Jesionowa 11, Wisznia Mała, 55-114, Poland.
Marycz, Krzysztof
  • Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Norwida 27B Street, A7 building, Wroclaw, 50-375, Poland. krzysztofmarycz@interia.pl.
  • International Institute of Translational Medicine, Malin, Jesionowa 11, Wisznia Mała, 55-114, Poland. krzysztofmarycz@interia.pl.
  • Faculty of Veterinary Medicine, Equine Clinic-Equine Surgery, Justus-Liebig-University, Giessen, 35392, Germany. krzysztofmarycz@interia.pl.

MeSH Terms

  • Alginates / pharmacology
  • Animals
  • Arthroscopy
  • Bone Cysts / diagnostic imaging
  • Bone Cysts / therapy
  • Bone Cysts / veterinary
  • Cell Proliferation
  • Cell Shape
  • Femur / drug effects
  • Femur / pathology
  • Horses
  • Hydrogels / pharmacology
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology
  • Sirtuin 1 / metabolism
  • Treatment Outcome

Conflict of Interest Statement

Authors declare that there is no conflict of interest.

References

This article includes 33 references
  1. Jeffcott L, Savage CJ. Nutrition and the development of Osteochondrosis (Dyschondroplasia). Pferdeheilkunde 1996;12:338–342.
    doi: 10.21836/PEM19960337google scholar: lookup
  2. Jeffcott LB. Osteochondrosis — An international problem for the horse industry. Journal of Equine Veterinary Science 1996;16(1):32–37.
    doi: 10.1016/S0737-0806(96)80063-3google scholar: lookup
  3. Bourebaba L, Röcken M, Marycz K. Osteochondritis dissecans (OCD) in Horses – Molecular Background of its Pathogenesis and Perspectives for Progenitor Stem Cell Therapy. Stem Cell Reviews and Reports 2019.
    doi: 10.1007/s12015-019-09875-6pmc: PMC6534522pubmed: 30796679google scholar: lookup
  4. Pettersson H, Sevelius F. Subchondral Bone Cysts in the Horse: a Clinical Study. Equine Veterinary Journal 1968;1(2):75–82.
    doi: 10.1111/j.2042-3306.1968.tb03350.xgoogle scholar: lookup
  5. von Rechenberg B, Guenther H, McIlwraith CW, Leutenegger C, Frisbie DD, Akens MK, Auer JA. Fibrous tissue of subchondral cystic lesions in horses produce local mediators and neutral metalloproteinases and cause bone resorption in vitro. Veterinary surgery: VS 2000;29(5):420–429.
    doi: 10.1053/jvet.2000.7538pubmed: 10999456google scholar: lookup
  6. von Rechenberg B, Leutenegger C, Zlinsky K, McIlwraith CW, Akens MK, Auer JA. Upregulation of mRNA of interleukin-1 and – 6 in subchondral cystic lesions of four horses. Equine Veterinary Journal 2001;33(2):143–149.
    doi: 10.1111/j.2042-3306.2001.tb00592.xpubmed: 11266063google scholar: lookup
  7. Manolagas SC, Jilka RL, Girasole G, Passeri G, Bellido T. Estrogen, cytokines, and the control of osteoclast formation and bone resorption in vitro and in vivo. Osteoporosis international: a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 1993;3(Suppl 1):114–116.
    doi: 10.1007/BF01621882pubmed: 8461536google scholar: lookup
  8. Kold SE, Hickman J, Melsen F. An experimental study of the healing process of equine chondral and osteochondral defects. Equine Veterinary Journal 1986;18(1):18–24.
    doi: 10.1111/j.2042-3306.1986.tb03529.xpubmed: 3948824google scholar: lookup
  9. Howard RD, McIlwraith CW, Trotter GW. Arthroscopic surgery for subchondral cystic lesions of the medial femoral condyle in horses: 41 cases (1988–1991). Journal of the American Veterinary Medical Association 1995;206(6):842–850.
    pubmed: 7759338
  10. Akyurekli C, Le Y, Richardson RB, Fergusson D, Tay J, Allan DS. A Systematic Review of Preclinical Studies on the Therapeutic Potential of Mesenchymal Stromal Cell-Derived Microvesicles. Stem Cell Reviews and Reports 2015;11(1):150–160.
    doi: 10.1007/s12015-014-9545-9pubmed: 25091427google scholar: lookup
  11. McIlwraith CW, Frisbie DD, Kawcak CE. The horse as a model of naturally occurring osteoarthritis. Bone & Joint Research 2012;1(11):297–309.
    doi: 10.1302/2046-3758.111.2000132pmc: PMC3626203pubmed: 23610661google scholar: lookup
  12. Cislo-Pakuluk A, Marycz K. A Promising Tool in Retina Regeneration: Current Perspectives and Challenges When Using Mesenchymal Progenitor Stem Cells in Veterinary and Human Ophthalmological Applications. Stem Cell Reviews 2017;13(5):598–602.
    doi: 10.1007/s12015-017-9750-4pmc: PMC5602072pubmed: 28643176google scholar: lookup
  13. Ghaderi A, Abtahi S. Mesenchymal Stem Cells: Miraculous Healers or Dormant Killers?. Stem Cell Reviews and Reports 2018;14(5):722–733.
    doi: 10.1007/s12015-018-9824-ypubmed: 29799101google scholar: lookup
  14. Gazdic M, Volarevic V, Arsenijevic N, Stojkovic M. Mesenchymal Stem Cells: A Friend or Foe in Immune-Mediated Diseases. Stem Cell Reviews and Reports 2015;11(2):280–287.
    doi: 10.1007/s12015-014-9583-3pubmed: 25592610google scholar: lookup
  15. Kornicka K, Houston J, Marycz K. Dysfunction of Mesenchymal Stem Cells Isolated from Metabolic Syndrome and Type 2 Diabetic Patients as Result of Oxidative Stress and Autophagy may Limit Their Potential Therapeutic Use. Stem Cell Reviews and Reports 2018;1–9.
    pmc: PMC5960487pubmed: 29611042doi: 10.1007/s12015-018-9809-xgoogle scholar: lookup
  16. Kornicka K, Marycz K, Tomaszewski KA, Marędziak M, Śmieszek A. The Effect of Age on Osteogenic and Adipogenic Differentiation Potential of Human Adipose Derived Stromal Stem Cells (hASCs) and the Impact of Stress Factors in the Course of the Differentiation Process. Oxidative Medicine and Cellular Longevity 2015;2015:309169.
    pmc: PMC4515302pubmed: 26246868doi: 10.1155/2015/309169google scholar: lookup
  17. Marycz K, Kornicka K, Basinska K, Czyrek A. Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging. Research article 2016.
    pmc: PMC4670679pubmed: 26682006doi: 10.1155/2016/4710326google scholar: lookup
  18. Kornicka K, Szłapka-Kosarzewska J, Śmieszek A, Marycz K. 5‐Azacytydine and resveratrol reverse senescence and ageing of adipose stem cells via modulation of mitochondrial dynamics and autophagy. Journal of Cellular and Molecular Medicine 2019;23(1):237–259.
    doi: 10.1111/jcmm.13914pmc: PMC6307768pubmed: 30370650google scholar: lookup
  19. Marycz K, Szłapka-Kosarzewska J, Geburek F, Kornicka-Garbowska K. Systemic Administration of Rejuvenated Adipose-Derived Mesenchymal Stem Cells Improves Liver Metabolism in Equine Metabolic Syndrome (EMS)- New Approach in Veterinary Regenerative Medicine. Stem Cell Reviews and Reports 2019.
    doi: 10.1007/s12015-019-09913-3pmc: PMC6925066pubmed: 31620992google scholar: lookup
  20. Bhullar KS, Hubbard BP. Lifespan and healthspan extension by resveratrol. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2015;1852(6):1209–1218.
    doi: 10.1016/j.bbadis.2015.01.012pubmed: 25640851google scholar: lookup
  21. Sun W, Qiao W, Zhou B, Hu Z, Yan Q, Wu J, Miao D. Overexpression of Sirt1 in mesenchymal stem cellsprotects against bone loss in mice by FOXO3a deacetylation and oxidative stressinhibition. Metabolism: Clinical and Experimental 2018;88:61–71.
    pubmed: 30318050doi: 10.1016/j.metabol.2018.06.006google scholar: lookup
  22. Golonka P, Szklarz M, Kusz M, Marędziak M, Irwin Houston JM, Marycz K. Subchondral bone cyst surgical treatment using the application of stem progenitor cells combined with alginate hydrogel in small joints in horses. Polish Journal of Veterinary Sciences 2018;21(2):307–316.
    doi: 10.24425/122598pubmed: 30450870google scholar: lookup
  23. Marycz K, Kornicka K, Irwin-Houston JM, Weiss C. Combination of resveratrol and 5-azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells. Journal of Cellular and Molecular Medicine 2018;22(10):4771–4793.
    doi: 10.1111/jcmm.13731pmc: PMC6156237pubmed: 29999247google scholar: lookup
  24. Oreffo ROC, Cooper C, Mason C, Clements M. Mesenchymal stem cells. Stem Cell Reviews 2005;1(2):169–178.
    doi: 10.1385/SCR:1:2:169pubmed: 17142852google scholar: lookup
  25. Piryaei A, Valojerdi MR, Shahsavani M, Baharvand H. Differentiation of Bone Marrow-derived Mesenchymal Stem Cells into Hepatocyte-like Cells on Nanofibers and Their Transplantation into a Carbon Tetrachloride-Induced Liver Fibrosis Model. Stem Cell Reviews and Reports 2011;7(1):103–118.
    doi: 10.1007/s12015-010-9126-5pubmed: 20182823google scholar: lookup
  26. Karamini A, Bakopoulou A, Andreadis D, Gkiouras K, Kritis A. Therapeutic Potential of Mesenchymal Stromal Stem Cells in Rheumatoid Arthritis: a Systematic Review of In Vivo Studies. Stem Cell Reviews and Reports 2020.
    doi: 10.1007/s12015-020-09954-zpubmed: 31950339google scholar: lookup
  27. Marędziak M, Śmieszek A, Chrząstek K, Basinska K, Marycz K. Physical Activity Increases the Total Number of Bone-Marrow-Derived Mesenchymal Stem Cells, Enhances Their Osteogenic Potential, and Inhibits Their Adipogenic Properties. Stem Cells International 2015.
    doi: 10.1155/2015/379093pmc: PMC4488015pubmed: 26167185google scholar: lookup
  28. Nawrocka D, Kornicka K, Śmieszek A, Marycz K. Spirulina platensis Improves Mitochondrial Function Impaired by Elevated Oxidative Stress in Adipose-Derived Mesenchymal Stromal Cells (ASCs) and Intestinal Epithelial Cells (IECs), and Enhances Insulin Sensitivity in Equine Metabolic Syndrome (EMS) Horses. Marine Drugs 2017;15(8).
    pmc: PMC5577592pubmed: 28771165doi: 10.3390/md15080237google scholar: lookup
  29. Marycz K, Toker N, Grzesiak J, Wrzeszcz K, Golonka P. The Therapeutic Effect of Autogenic Adipose Derived Stem Cells Combined with Autogenic Platelet Rich Plasma in Tendons Disorders in Horses in vitro and in vivo Research. Journal of Animal and Veterinary Advances 2012;11:4324–4331.
    doi: 10.3923/javaa.2012.4324.4331google scholar: lookup
  30. Marycz K, Kornicka K, Grzesiak J, Śmieszek A, Szłapka J. Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation. Oxidative Medicine and Cellular Longevity 2016.
    doi: 10.1155/2016/3718468pmc: PMC5178365pubmed: 28053691google scholar: lookup
  31. Kornicka K, Śmieszek A, Węgrzyn AS, Röcken M, Marycz K. Immunomodulatory Properties of Adipose-Derived Stem Cells Treated with 5-Azacytydine and Resveratrol on Peripheral Blood Mononuclear Cells and Macrophages in Metabolic Syndrome Animals. Journal of Clinical Medicine 2018;7(11):383.
    doi: 10.3390/jcm7110383pmc: PMC6262510pubmed: 30356025google scholar: lookup
  32. Choi Y, Yoon DS, Lee KM, Choi SM, Lee MH, Park KH, Lee JW. Enhancement of Mesenchymal StemCell-Driven Bone Regeneration by Resveratrol-Mediated SOX2 Regulation. Aging and Disease 2019;10(4):818–833.
    pmc: PMC6675538pubmed: 31440387doi: 10.14336/ad.2018.0802google scholar: lookup
  33. Yan X, Ehnert S, Culmes M, Bachmann A, Seeliger C, Schyschka L, Nussler AK. 5-azacytidine improvesthe osteogenic differentiation potential of aged human adipose-derivedmesenchymal stem cells by DNA demethylation. PloS One 2014;9(6):e90846.
    pmc: PMC3946260pubmed: 24603866doi: 10.1371/journal.pone.0090846google scholar: lookup

Citations

This article has been cited 2 times.
  1. Suresh N, Lekhavadhani S, Selvamurugan N. Advances in polymer-based hydrogel systems for adipose-derived mesenchymal stem cells toward bone regeneration. World J Orthop 2026 Jan 18;17(1):113228.
    doi: 10.5312/wjo.v17.i1.113228pubmed: 41608499google scholar: lookup
  2. Pielok A, Kępska M, Steczkiewicz Z, Grobosz S, Bourebaba L, Marycz K. Equine Hoof Progenitor Cells Display Increased Mitochondrial Metabolism and Adaptive Potential to a Highly Pro-Inflammatory Microenvironment. Int J Mol Sci 2023 Jul 14;24(14).
    doi: 10.3390/ijms241411446pubmed: 37511204google scholar: lookup
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