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Cell proliferation2011; 44(4); 330-342; doi: 10.1111/j.1365-2184.2011.00759.x

Size-sieved subpopulations of mesenchymal stem cells from intervascular and perivascular equine umbilical cord matrix.

Abstract: Umbilical cord matrix (UCM) has been recently proposed as an alternative source of mesenchymal stem cells (MSCs). The aim of this study was to isolate and characterize presumptive stem cells from intervascular and perivascular equine UCM and to obtain homogeneous subpopulations from both sites. Methods: Umbilical cords were processed for retrieval of MSCs. Unsieved cells from intervascular and perivascular portions were evaluated for cell cycle analysis and for immunophenotyping by flow cytometry. Cells from each site were separated into larger and smaller sieved populations using multi-dishes with 8-μm pore transwell inserts. Each cell population was characterized in terms of renewal capability, specific marker expression and differentiation potential. Cryopreservation was performed on sieved cells only. Results: Cells from both areas expressed MSC and pluripotential specific markers and were able to differentiate into mesodermic and ectodermic lineages. The sieving procedure yielded two relatively homogeneous subpopulations with comparable characteristics. Surprisingly, after sieving, large intervascular and small perivascular cells were the most rapidly replicating cells [20.53 and 19.49 cell population doublings (PD) after 31 days respectively] and also showed higher fibroblast colony forming unit frequency. Unsieved cell populations were used as controls, and showed PD of 9.42(intervascular cells) and 8.54 (perivascular cells) after 31 days. Conclusions: Here, cells from UCM represented an intermediate stage between pluripotent embryonic and adult stem cells. Size-sieving can be used to isolate more rapidly proliferating cell populations.
© 2011 Blackwell Publishing Ltd.
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Publication Date: 2011-06-06 PubMed ID: 21645152PubMed Central: PMC6496711DOI: 10.1111/j.1365-2184.2011.00759.xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on isolating and characterizing stem cells from intervascular and perivascular regions of the equine umbilical cord matrix (UCM). It compares the features of size-sieved populations from both locations, establishing that size-sieving can identify sub-populations that proliferate more rapidly.

Methods

  • Umbilical cords were processed to extract mesenchymal stem cells (MSCs).
  • Unsieved cells from intervascular and perivascular regions were subjected to cell cycle analysis and immunophenotyping using flow cytometry.
  • Cells were sorted into larger and smaller populations using multi-dishes with 8-μm pore transwell inserts.
  • All sub-populations were studied for renewal ability, specific marker expression and differentiation potential. Cryopreservation was exclusively conducted on sieved cells.

Results

  • Cells from both regions expressed markers of MSC and pluripotential cells, and could differentiate into mesodermic and ectodermic lineages.
  • The sieving method produced two relatively consistent sub-populations with analogous characteristics.
  • After sieving, large intervascular and small perivascular cells demonstrated the fastest replication — 20.53 and 19.49 cell population doublings (PD) respectively, after 31 days.
  • These cells also displayed higher fibroblast colony forming unit frequency.
  • Control unsieved cell populations showed PD of 9.42 (intervascular cells) and 8.54 (perivascular cells) after similar time span.

Conclusions

  • The cells derived from UCM represented an intermediate stage between pluripotent embryonic and adult stem cells.
  • Size sieving method can be employed to isolate populations that demonstrate higher proliferative capabilities.

Cite This Article

APA
Corradetti B, Lange-Consiglio A, Barucca M, Cremonesi F, Bizzaro D. (2011). Size-sieved subpopulations of mesenchymal stem cells from intervascular and perivascular equine umbilical cord matrix. Cell Prolif, 44(4), 330-342. https://doi.org/10.1111/j.1365-2184.2011.00759.x

Publication

Cell proliferation
ISSN: 1365-2184
NlmUniqueID: 9105195
Country: England
Language: English
Volume: 44
Issue: 4
Pages: 330-342

Researcher Affiliations

Corradetti, B
  • Università Politecnica delle Marche, Department of Biochemistry, Biology and Genetics, Ancona, Italy.
Lange-Consiglio, A
    Barucca, M
      Cremonesi, F
        Bizzaro, D

          MeSH Terms

          • Animals
          • Cell Proliferation
          • Cell Separation / methods
          • Cell Size
          • Female
          • Flow Cytometry
          • Horses
          • Mesenchymal Stem Cells / cytology
          • Umbilical Cord / cytology

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