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Home / Equine Research Bank / Biology (Basel) / Structure and Strength of Bovine and Equine Amniotic Membran...
Biology2022; 11(8); 1096; doi: 10.3390/biology11081096

Structure and Strength of Bovine and Equine Amniotic Membrane.

Abstract: Thin, strong scaffold materials are needed for surgical applications. New materials are required, particularly those readily available, such as from non-human sources. Bovine amniotic membrane (antepartum) and equine amniotic membrane (postpartum) were characterized with tear and tensile tests. The structural arrangement of the collagen fibrils was determined by small-angle X-ray scattering, scanning electron microscopy, and ultrasonic imaging. Bovine amnion had a thickness-normalized tear strength of 12.6 (3.8) N/mm, while equine amnion was 14.8 (5.3) N/mm. SAXS analysis of the collagen fibril arrangement yielded an orientation index of 0.587 (0.06) and 0.681 (0.05) for bovine and equine, respectively. This may indicate a relationship between more highly aligned collagen fibrils and greater strength, as seen in other materials. Amnion from bovine or equine sources are strong, thin, elastic materials, although weaker than other collagen tissue materials commonly used, that may find application in surgery as an alternative to material from human donors.
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Publication Date: 2022-07-23 PubMed ID: 35892952PubMed Central: PMC9329871DOI: 10.3390/biology11081096Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated the structure and strength of bovine (cow) and equine (horse) amniotic membranes with potential surgical applications. These thin, strong materials from non-human sources could prove useful in creating new supplies for various medical procedures.

Analysis and Testing Methods

  • The study tested the tear and tensile strength of both bovine (antepartum) and equine (postpartum) amniotic membranes. Tear and tensile tests measure the force required to pull a material apart, indicating its resistance to tearing and its overall strength respectively.
  • The researchers used small-angle X-ray scattering (SAXS), scanning electron microscopy, and ultrasonic imaging to determine the structural arrangement of collagen fibrils. Collagen fibrils, a necessary component of connective tissues in essentially all multi-cellular organisms, are the primary constituent of these membranes and their arrangement is fundamentally related to their functional properties.

Findings from the Study

  • The bovine amnion showed a thickness-normalized tear strength of 12.6 (3.8) N/mm, while the equine amnion measured at 14.8 (5.3) N/mm. These values suggest that these membranes are quite resilient and capable of withstanding substantial forces.
  • SAXS analysis showed that the collagen fibril arrangement in both bovine and equine amniotic membranes were highly aligned. Bovine amniotic membrane had an orientation index of 0.587 (0.06), while the equine amniotic membrane measured at 0.681 (0.05). This high degree of alignment would generally imply that the material would exhibit strong tensile strength.
  • The findings suggest a possible relationship between the greater alignment of collagen fibrils and increased strength in these membranes, a trend identified in other material studies.

Implications for Surgical Applications

  • Amnion from bovine or equine sources are strong, thin, elastic materials, even though they are weaker than other commonly used collagen tissue materials.
  • These properties make them potential candidates for use in surgical procedures as an alternative to materials from human donors. Grafting these tissues into humans may provide a significant advantage in their availability as they can be regularly collected from animals without ethical or supply concerns associated with human donors.

Cite This Article

APA
Wells HC, Sizeland KH, Kirby N, Haverkamp RG. (2022). Structure and Strength of Bovine and Equine Amniotic Membrane. Biology (Basel), 11(8), 1096. https://doi.org/10.3390/biology11081096

Publication

Biology
ISSN: 2079-7737
NlmUniqueID: 101587988
Country: Switzerland
Language: English
Volume: 11
Issue: 8
PII: 1096

Researcher Affiliations

Wells, Hannah C
  • School of Food and Advanced Technology, Massey University, Palmerston North 4442, New Zealand.
Sizeland, Katie H
  • ANSTO, Lucas Heights, NSW 2234, Australia.
  • ANSTO, Clayton, VIC 3168, Australia.
Kirby, Nigel
  • ANSTO, Clayton, VIC 3168, Australia.
Haverkamp, Richard G
  • School of Food and Advanced Technology, Massey University, Palmerston North 4442, New Zealand.

Grant Funding

  • M10652 / Australian Nuclear Science and Technology Organisation

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 46 references
  1. Calvin SE, Oyen ML. Microstructure and mechanics of the chorioamnion membrane with an emphasis on fracture properties.. Ann N Y Acad Sci 2007 Apr;1101:166-85.
    doi: 10.1196/annals.1389.009pubmed: 17332077google scholar: lookup
  2. Koob TJ, Lim JJ, Massee M, Zabek N, Denozière G. Properties of dehydrated human amnion/chorion composite grafts: Implications for wound repair and soft tissue regeneration.. J Biomed Mater Res B Appl Biomater 2014 Aug;102(6):1353-62.
    doi: 10.1002/jbm.b.33141pubmed: 24664953google scholar: lookup
  3. Leal-Marin S, Kern T, Hofmann N, Pogozhykh O, Framme C, Börgel M, Figueiredo C, Glasmacher B, Gryshkov O. Human Amniotic Membrane: A review on tissue engineering, application, and storage.. J Biomed Mater Res B Appl Biomater 2021 Aug;109(8):1198-1215.
    doi: 10.1002/jbm.b.34782pubmed: 33319484google scholar: lookup
  4. Arrizabalaga JH, Nollert MU. Human Amniotic Membrane: A Versatile Scaffold for Tissue Engineering.. ACS Biomater Sci Eng 2018 Jul 9;4(7):2226-2236.
    doi: 10.1021/acsbiomaterials.8b00015pubmed: 33435098google scholar: lookup
  5. von Versen-Höynck F, Hesselbarth U, Möller DE. Application of sterilised human amnion for reconstruction of the ocular surface.. Cell Tissue Bank 2004;5(1):57-65.
    doi: 10.1023/B:CATB.0000022222.41304.depubmed: 15256840google scholar: lookup
  6. Jirsova K, Jones GLA. Amniotic membrane in ophthalmology: properties, preparation, storage and indications for grafting-a review.. Cell Tissue Bank 2017 Jun;18(2):193-204.
    doi: 10.1007/s10561-017-9618-5pubmed: 28255771google scholar: lookup
  7. Riordan NH, George BA, Chandler TB, McKenna RW. Case report of non-healing surgical wound treated with dehydrated human amniotic membrane.. J Transl Med 2015 Jul 24;13:242.
    doi: 10.1186/s12967-015-0608-8pmc: PMC4513638pubmed: 26205894google scholar: lookup
  8. Monavarian M, Kader S, Moeinzadeh S, Jabbari E. Regenerative Scar-Free Skin Wound Healing.. Tissue Eng Part B Rev 2019 Aug;25(4):294-311.
    doi: 10.1089/ten.teb.2018.0350pmc: PMC6686695pubmed: 30938269google scholar: lookup
  9. Castellanos G, Bernabé-García Á, Moraleda JM, Nicolás FJ. Amniotic membrane application for the healing of chronic wounds and ulcers.. Placenta 2017 Nov;59:146-153.
    doi: 10.1016/j.placenta.2017.04.005pubmed: 28413063google scholar: lookup
  10. Li W, Ma G, Brazile B, Li N, Dai W, Butler JR, Claude AA, Wertheim JA, Liao J, Wang B. Investigating the Potential of Amnion-Based Scaffolds as a Barrier Membrane for Guided Bone Regeneration.. Langmuir 2015 Aug 11;31(31):8642-53.
    doi: 10.1021/acs.langmuir.5b02362pubmed: 26158559google scholar: lookup
  11. Elahi A, Taib H, Berahim Z, Ahmad A, Hamid SSA, Mocktar NA. Amniotic membrane as a scaffold for periodontal tissue engineering.. J. Health Sci. Med. Res. 2021;39:169–180.
    doi: 10.31584/jhsmr.2020768google scholar: lookup
  12. Gulameabasse S, Gindraux F, Catros S, Fricain JC, Fenelon M. Chorion and amnion/chorion membranes in oral and periodontal surgery: A systematic review.. J Biomed Mater Res B Appl Biomater 2021 Aug;109(8):1216-1229.
    doi: 10.1002/jbm.b.34783pubmed: 33354857google scholar: lookup
  13. Ramuta TŽ, Kreft ME. Human Amniotic Membrane and Amniotic Membrane-Derived Cells: How Far Are We from Their Use in Regenerative and Reconstructive Urology?. Cell Transplant 2018 Jan;27(1):77-92.
    doi: 10.1177/0963689717725528pmc: PMC6434475pubmed: 29562770google scholar: lookup
  14. Maan ZN, Rennert RC, Koob TJ, Januszyk M, Li WW, Gurtner GC. Cell recruitment by amnion chorion grafts promotes neovascularization.. J Surg Res 2015 Feb;193(2):953-962.
    doi: 10.1016/j.jss.2014.08.045pmc: PMC4425288pubmed: 25266600google scholar: lookup
  15. Song M, Wang W, Ye Q, Bu S, Shen Z, Zhu Y. The repairing of full-thickness skin deficiency and its biological mechanism using decellularized human amniotic membrane as the wound dressing.. Mater Sci Eng C Mater Biol Appl 2017 Aug 1;77:739-747.
    doi: 10.1016/j.msec.2017.03.232pubmed: 28532087google scholar: lookup
  16. Cui H, Chai Y, Yu Y. Progress in developing decellularized bioscaffolds for enhancing skin construction.. J Biomed Mater Res A 2019 Aug;107(8):1849-1859.
    doi: 10.1002/jbm.a.36688pubmed: 30942934google scholar: lookup
  17. Heath DE. A Review of Decellularized Extracellular Matrix Biomaterials for Regenerative Engineering Applications.. Regen. Eng. Transl. Med. 2019;5:155–166.
    doi: 10.1007/s40883-018-0080-0google scholar: lookup
  18. Wells HC, Sizeland KH, Kirby N, Hawley A, Mudie S, Haverkamp RG. Collagen Fibril Structure and Strength in Acellular Dermal Matrix Materials of Bovine, Porcine, and Human Origin.. ACS Biomater Sci Eng 2015 Oct 12;1(10):1026-1038.
    doi: 10.1021/acsbiomaterials.5b00310pubmed: 33429533google scholar: lookup
  19. Patel S, Ziai K, Lighthall JG, Walen SG. Biologics and acellular dermal matrices in head and neck reconstruction: A comprehensive review.. Am J Otolaryngol 2022 Jan-Feb;43(1):103233.
    doi: 10.1016/j.amjoto.2021.103233pubmed: 34537508google scholar: lookup
  20. Kim JY, Choi YM, Jeong SW, Williams DL. Effect of bovine freeze-dried amniotic membrane (Amnisite-BA) on uncomplicated canine corneal erosion.. Vet Ophthalmol 2009 Jan-Feb;12(1):36-42.
    doi: 10.1111/j.1463-5224.2009.00671.xpubmed: 19152596google scholar: lookup
  21. Ollivier FJ, Kallberg ME, Plummer CE, Barrie KP, O'Reilly S, Taylor DP, Gelatt KN, Brooks DE. Amniotic membrane transplantation for corneal surface reconstruction after excision of corneolimbal squamous cell carcinomas in nine horses.. Vet Ophthalmol 2006 Nov-Dec;9(6):404-13.
    doi: 10.1111/j.1463-5224.2006.00480.xpubmed: 17076873google scholar: lookup
  22. Tsuzuki K, Yamashita K, Izumisawa Y, Kotani T. Microstructure and glycosaminoglycan ratio of canine cornea after reconstructive transplantation with glycerin-preserved porcine amniotic membranes.. Vet Ophthalmol 2008 Jul-Aug;11(4):222-7.
    doi: 10.1111/j.1463-5224.2008.00629.xpubmed: 18638347google scholar: lookup
  23. Barros PS, Safatle AM, Godoy CA, Souza MS, Barros LF, Brooks DE. Amniotic membrane transplantation for the reconstruction of the ocular surface in three cases.. Vet Ophthalmol 2005 May-Jun;8(3):189-92.
    doi: 10.1111/j.1463-5224.2005.00391.xpubmed: 15910372google scholar: lookup
  24. Withavatpongtorn N, Tuntivanich N. Characterization of Cryopreserved Canine Amniotic Membrane.. Membranes (Basel) 2021 Oct 27;11(11).
    doi: 10.3390/membranes11110824pmc: PMC8624976pubmed: 34832052google scholar: lookup
  25. Oxlund H, Helmig R, Halaburt JT, Uldbjerg N. Biomechanical analysis of human chorioamniotic membranes.. Eur J Obstet Gynecol Reprod Biol 1990 Mar;34(3):247-55.
    doi: 10.1016/0028-2243(90)90078-Fpubmed: 2311812google scholar: lookup
  26. Moore RM, Mansour JM, Redline RW, Mercer BM, Moore JJ. The physiology of fetal membrane rupture: insight gained from the determination of physical properties.. Placenta 2006 Nov-Dec;27(11-12):1037-51.
    doi: 10.1016/j.placenta.2006.01.002pubmed: 16516962google scholar: lookup
  27. Bircher K, Ehret AE, Spiess D, Ehrbar M, Simões-Wüst AP, Ochsenbein-Kölble N, Zimmermann R, Mazza E. On the defect tolerance of fetal membranes.. Interface Focus 2019 Oct 6;9(5):20190010.
    doi: 10.1098/rsfs.2019.0010pmc: PMC6710661pubmed: 31485307google scholar: lookup
  28. Pressman EK, Cavanaugh JL, Woods JR. Physical properties of the chorioamnion throughout gestation.. Am J Obstet Gynecol 2002 Sep;187(3):672-5.
    doi: 10.1067/mob.2002.125742pubmed: 12237646google scholar: lookup
  29. Helmig R, Oxlund H, Petersen LK, Uldbjerg N. Different biomechanical properties of human fetal membranes obtained before and after delivery.. Eur J Obstet Gynecol Reprod Biol 1993 Mar;48(3):183-9.
    doi: 10.1016/0028-2243(93)90086-Rpubmed: 8335136google scholar: lookup
  30. Benson-Martin J, Zammaretti P, Bilic G, Schweizer T, Portmann-Lanz B, Burkhardt T, Zimmermann R, Ochsenbein-Kölble N. The Young's modulus of fetal preterm and term amniotic membranes.. Eur J Obstet Gynecol Reprod Biol 2006 Sep-Oct;128(1-2):103-7.
    doi: 10.1016/j.ejogrb.2005.12.011pubmed: 16442204google scholar: lookup
  31. Baird PN, Machin H, Brown KD. Corneal supply and the use of technology to reduce its demand: A review.. Clin Exp Ophthalmol 2021 Dec;49(9):1078-1090.
    doi: 10.1111/ceo.13978pubmed: 34310836google scholar: lookup
  32. Sacks MS, Smith DB, Hiester ED. A small angle light scattering device for planar connective tissue microstructural analysis.. Ann Biomed Eng 1997 Jul-Aug;25(4):678-89.
    doi: 10.1007/BF02684845pubmed: 9236980google scholar: lookup
  33. Cookson D, Kirby N, Knott R, Lee M, Schultz D. Strategies for data collection and calibration with a pinhole-geometry SAXS instrument on a synchrotron beamline.. J Synchrotron Radiat 2006 Nov;13(Pt 6):440-4.
    doi: 10.1107/S0909049506030184pubmed: 17057319google scholar: lookup
  34. Chua WK, Oyen ML. Do we know the strength of the chorioamnion? A critical review and analysis.. Eur J Obstet Gynecol Reprod Biol 2009 May;144 Suppl 1:S128-33.
    doi: 10.1016/j.ejogrb.2009.02.029pubmed: 19286299google scholar: lookup
  35. Manabe Y, Himeno N, Fukumoto M. Tensile strength and collagen content of amniotic membrane do not change after the second trimester or during delivery.. Obstet Gynecol 1991 Jul;78(1):24-7.
    pubmed: 2047062
  36. Sizeland KH, Wells HC, Higgins J, Cunanan CM, Kirby N, Hawley A, Mudie ST, Haverkamp RG. Age dependent differences in collagen alignment of glutaraldehyde fixed bovine pericardium.. Biomed Res Int 2014;2014:189197.
    doi: 10.1155/2014/189197pmc: PMC4180201pubmed: 25295250google scholar: lookup
  37. Kayed HR, Sizeland KH, Wells HC, Kirby N, Hawley A, Mudie S, Haverkamp RG. Age differences with glutaraldehyde treatment in collagen fibril orientation of bovine pericardium.. J. Biomater. Tissue Eng. 2016;6:992–997.
    doi: 10.1166/jbt.2016.1532google scholar: lookup
  38. Kayed HR, Sizeland KH, Kirby N, Hawley A, Mudie ST, Haverkamp RG. Collagen cross linking and fibril alignment in pericardium.. RSC Adv. 2015;5:3611–3618.
    doi: 10.1039/C4RA10658Jgoogle scholar: lookup
  39. Seto J, Gupta HS, Zaslansky P, Wagner HD, Fratzl P. Tough lessons from bone: Extreme mechanical anisotropy at the mesoscale.. Adv. Funct. Mater. 2008;18:1905–1911.
    doi: 10.1002/adfm.200800214google scholar: lookup
  40. Wells HC, Sizeland KH, Kayed HR, Kirby N, Hawley A, Mudie ST, Haverkamp RG. Poisson’s ratio of collagen fibrils measured by SAXS of strained bovine pericardium.. J. Appl. Phys. 2015;117:044701.
    doi: 10.1063/1.4906325google scholar: lookup
  41. Sizeland KH, Basil-Jones MM, Edmonds RL, Cooper SM, Kirby N, Hawley A, Haverkamp RG. Collagen orientation and leather strength for selected mammals.. J Agric Food Chem 2013 Jan 30;61(4):887-92.
    doi: 10.1021/jf3043067pubmed: 23298142google scholar: lookup
  42. Basil-Jones MM, Edmonds RL, Norris GE, Haverkamp RG. Collagen fibril alignment and deformation during tensile strain of leather: a small-angle X-ray scattering study.. J Agric Food Chem 2012 Feb 8;60(5):1201-8.
    doi: 10.1021/jf2039586pubmed: 22233427google scholar: lookup
  43. Yang W, Sherman VR, Gludovatz B, Schaible E, Stewart P, Ritchie RO, Meyers MA. On the tear resistance of skin.. Nat Commun 2015 Mar 27;6:6649.
    doi: 10.1038/ncomms7649pmc: PMC4389263pubmed: 25812485google scholar: lookup
  44. Haverkamp RG, Williams MA, Scott JE. Stretching single molecules of connective tissue glycans to characterize their shape-maintaining elasticity.. Biomacromolecules 2005 May-Jun;6(3):1816-8.
    doi: 10.1021/bm0500392pubmed: 15877410google scholar: lookup
  45. Haugh MG, Jaasma MJ, O'Brien FJ. The effect of dehydrothermal treatment on the mechanical and structural properties of collagen-GAG scaffolds.. J Biomed Mater Res A 2009 May;89(2):363-9.
    doi: 10.1002/jbm.a.31955pubmed: 18431763google scholar: lookup
  46. Oxlund H, Manschot J, Viidik A. The role of elastin in the mechanical properties of skin.. J Biomech 1988;21(3):213-8.
    doi: 10.1016/0021-9290(88)90172-8pubmed: 3379082google scholar: lookup

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