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Home / Equine Research Bank / Ir Vet J / Tamoxifen inhibits chemokinesis in equine neutrophils.
Irish veterinary journal2018; 71; 22; doi: 10.1186/s13620-018-0133-1

Tamoxifen inhibits chemokinesis in equine neutrophils.

Abstract: Neutrophils are terminally differentiated innate effector cells at the first line of host defense. Neutrophil migration within tissues is complex and involves several steps, during which these cells must be able to interpret a variety of chemical and physical signals. Exacerbated neutrophil activity can be harmful to surrounding tissues; this is important in a range of diseases, including equine asthma. Tamoxifen (TX) is a non-steroidal estrogen receptor modulator with effects on cell growth and survival. Previous studies showed that TX treatment in horses with induced acute pulmonary inflammation promoted early apoptosis of blood and bronchoalveolar lavage fluid (BALF) neutrophils, reduction of BALF neutrophil content, and improvement in animals' clinical status. Further, TX dampens chemotactic index and respiratory burst production in vitro. The aim of this study was to provide information on the effect of TX on chemokinesis in peripheral blood neutrophils from five healthy horses. Results showed that neutrophils increased migration and travelled distance in response to IL-8; but in the presence of TX, IL-8 did not produce neutrophil migration. This suggests that TX has an inhibitory effect on the kinesis of equine peripheral blood neutrophils stimulated with IL-8. However, further studies are required to fully understand the signaling pathways of TX on neutrophil chemokinesis.
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Publication Date: 2018-10-23 PubMed ID: 30386589PubMed Central: PMC6199699DOI: 10.1186/s13620-018-0133-1Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates how Tamoxifen (TX), a non-steroidal estrogen receptor modulator, affects the movement of neutrophils, a type of immune cell, in horses. The results indicate that TX can inhibit these immune cells’ activity, which may have implications for equine asthma treatment.

Background

  • Neutrophils are critical components of the immune system, representing the first line of defense against infections.
  • However, excessive neutrophil activity can damage surrounding tissues, leading to various diseases such as equine asthma.
  • Tamoxifen (TX), typically used as a hormone therapy for breast cancer in humans, is known to affect cell growth and survival.
  • Past studies have indicated that TX can promote early apoptosis (programmed cell death) in neutrophils, reduce neutrophil content in the lung (as measured in bronchoalveolar lavage fluid, BALF), and improve the clinical condition of horses with induced acute pulmonary inflammation.
  • Additionally, TX has shown potential to reduce neutrophil chemotactic index (a measure of cell movement towards a chemical stimulus) and respiratory burst production (a crucial immune response of neutrophils).

Aim and Methodology

  • The study aimed to analyze TX’s effect on chemokinesis (random or non-directional cell movement) of neutrophils in the blood of healthy horses.
  • The authors collected peripheral blood neutrophils from five healthy horses and measured their response to IL-8, a potent chemoattractant, in the presence and absence of TX.

Results and Interpretation

  • The study found that neutrophils noticeably increased their migration and travelled distance in response to IL-8 signaling.
  • However, when TX was present, IL-8 did not induce neutrophil migration, indicating that TX has an inhibitory effect on neutrophil chemokinesis.
  • This suggests that TX might be useful in controlling harmful neutrophil activities in equine asthma and other diseases involving excessive neutrophil activity.

Further Studies

  • While these results are promising, further research is needed to better understand the specific signaling pathways by which TX affects neutrophil chemokinesis. This will help establish TX’s full potential in equine disease management.

Cite This Article

APA
Morales N, Henriquez C, Sarmiento J, Uberti B, Moran G. (2018). Tamoxifen inhibits chemokinesis in equine neutrophils. Ir Vet J, 71, 22. https://doi.org/10.1186/s13620-018-0133-1

Publication

Irish veterinary journal
ISSN: 0368-0762
NlmUniqueID: 0100762
Country: Ireland
Language: English
Volume: 71
Pages: 22
PII: 22

Researcher Affiliations

Morales, Natalia
  • 1Department of Pharmacology, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile.
Henriquez, Claudio
  • 1Department of Pharmacology, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile.
Sarmiento, Jose
  • 2Department of Physiology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile.
Uberti, Benjamin
  • 3Department of Veterinary Clinical Sciences, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile.
Moran, Gabriel
  • 1Department of Pharmacology, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile.

Conflict of Interest Statement

All experimental procedures were approved by the Universidad Austral de Chile Bioethics Committee for the Use of Animals in Biomedical Research.Not applicable.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Citations

This article has been cited 3 times.
  1. Salinas C, Barriga K, Albornoz A, Alarcon P, Quiroga J, Uberti B, Sarmiento J, Henriquez C, Ehrenfeld P, Burgos RA, Moran G. Tamoxifen triggers the in vitro release of neutrophil extracellular traps in healthy horses.. Front Vet Sci 2022;9:1025249.
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  2. Albornoz A, Morales N, Uberti B, Henriquez C, Burgos RA, Alarcon P, Moran G. Tamoxifen and its metabolites induce mitochondrial membrane depolarization and caspase-3 activation in equine neutrophils.. Vet Med Sci 2020 Nov;6(4):673-678.
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  3. Gajardo G, López-Muñoz R, Plaza A, Uberti B, Sarmiento J, Morán G, Henríquez C. Tamoxifen in horses: pharmacokinetics and safety study.. Ir Vet J 2019;72:5.
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