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The distribution of nerve fibres immunoreactive for vasoactive intestinal peptide, calcitonin gene-related peptide, substance P and dopamine beta-hydroxylase in the normal equine larynx.
Abstract: The autonomic innervation of the mammalian respiratory system is complex, and involves a wide variety of peptide and non-peptide neurotransmitters which will have an important role in normal laryngeal function and the response to disease. This innervation has been partially described in the horse airway and lung, but there is no information on the equine larynx. This paper describes the expression and distribution of nerve fibres immunoreactive for vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), substance P (SP) and the adrenergic enzymatic marker dopamine beta-hydroxylase (DBetaH) in the mucosa of the equine larynx. The overall relative density of nerve fibres immunoreactive for the different antigens was VIP>>CGRP>SP>>DBetaH. There were differences in the distribution of nerve fibre types, although each antigen was found in nerve fibres adjacent to blood vessels and mucous glands. VIP -like immunoreactivity (VIP -Li) was particularly extensive in association with mucous glands. SP - and CGRP -like immunoreactivity (SP -Li, CGRP -Li) were also seen close to the epithelium, with occasional nerve fibres coursing beneath and between the epithelial cells. Fragments of SP -Li and CGRP -Li fibres were also present in large nerve fibre bundles and ganglionic cell clusters, but not in the neurons themselves. The density of nerve fibres immunoreactive for DBetaH was very low and restricted to blood vessels and mucous glands. There was marked variation in the density of nerve fibres at the different sites, with the greatest density, particularly for VIP, over the arytenoid cartilage. Immunoreactive nerve fibres were less plentiful over the epiglottis, and the density of all types of nerve fibres was low over the cricoid cartilage. Overall VIP -Li nerve fibres were the most plentiful.
Copyright 1999 Harcourt Publishers Limited.
Publication Date: 1999-12-23 PubMed ID: 10607505DOI: 10.1053/rvsc.1999.0325Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study examines the distribution of nerve fibers in the equine larynx that produce certain neurotransmitters, in order to improve our understanding of the functioning and diseases of the respiratory system. The neurotransmitters studied are vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), substance P (SP) and dopamine beta-hydroxylase (DBetaH).
Research Methodology
- The researchers examined the equine larynx and collected samples of the mucosa, which were then examined and analysed to identify and count nerve fibers reactive with each of the neurotransmitters – VIP, CGRP, SP, and DBetaH.
- Their density was determined and the nerve fibers were charted to determine their distribution.
Results and Findings
- The study found that the highest density of nerve fibers was immunoreactive for VIP, followed by CGRP and SP, and the lowest for DBetaH.
- Despite the different densities, each of these neurotransmitters was found in the nerve fibers of blood vessels and mucous glands in the larynx.
- VIP showed a particularly extensive immunoreactivity associated with mucous glands, while SP and CGRP immunoreactivity were also noted near the epithelium.
- SP and CGRP positive nerve fibers were also identified between and beneath the epithelial cells.
- However, DBetaH immunoreactive nerve fibers were rarer and restricted to blood vessels and mucous glands.
Distribution of Nerve Fibers
- The greatest nerve fiber density, particularly for VIP, was found over the arytenoid cartilage of the larynx.
- Immunoreactive nerve fibers were less abundant on the epiglottis, and the overall nerve fiber density was low over the cricoid cartilage.
- Overall, the most abundant nerve fibers were VIP immunoreactive.
In conclusion, this study provides insights into the autonomic innervation of the equine larynx and its role in laryngeal function and disease. This distribution and density of nerve fibers gives us a better understanding of the role of these neurotransmitters in normal function and disease processes.
Cite This Article
APA
Corcoran BM, Jarvis S, Hahn CN, Mayhew IG.
(1999).
The distribution of nerve fibres immunoreactive for vasoactive intestinal peptide, calcitonin gene-related peptide, substance P and dopamine beta-hydroxylase in the normal equine larynx.
Res Vet Sci, 67(3), 251-259.
https://doi.org/10.1053/rvsc.1999.0325 Publication
Researcher Affiliations
- Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Summerhall, Edinburgh, EH9 1QH. BMC@ed.ac.uk
MeSH Terms
- Animals
- Calcitonin Gene-Related Peptide / analysis
- Dopamine beta-Hydroxylase / analysis
- Horses / anatomy & histology
- Horses / metabolism
- Intestinal Mucosa / chemistry
- Laryngeal Nerves / chemistry
- Substance P / analysis
- Vasoactive Intestinal Peptide / analysis
Citations
This article has been cited 3 times.- Isaka M, Ueno H. Vasoactive intestinal peptide in canine portosystemic shunt in the absence of portal hypertension. Open Vet J 2021 Jan-Mar;11(1):39-41.
- Foote AG, Thibeault SL. Sensory Innervation of the Larynx and the Search for Mucosal Mechanoreceptors. J Speech Lang Hear Res 2021 Feb 17;64(2):371-391.
- Yamazaki M, Sato I. Distribution of substance P and the calcitonin gene-related peptide in the human tensor tympani muscle. Eur Arch Otorhinolaryngol 2014 May;271(5):905-11.
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