The Effect of a Seaweed-Derived Calcium Supplement on Gastric Juice pH in the Horse.

This study investigates the effects of a seaweed-based calcium supplement on the pH level of gastric juice in horses, finding that the supplementation increases the buffering capacities two hours after feeding.

Research Context and Objective

• The primary focus of this study was to understand if the introduction of a seaweed-derived calcium supplement could be beneficial in managing gastric acid levels in horses. Extended periods of low gastric pH (acidic conditions) can increase the risk of gastric ulcerations in horses, so nutritional interventions like this could help mitigate this risk.
• The specific calcium supplement used in this study was derived from two types of red marine algae, Lithothamnion corallioides and Phymatolithon calcareum, commercially known as Calmin.

Experimental Design and Method

• Nine mature, Thoroughbred-cross horses were the sample group for this study. They were fed a specific diet which was comprised of a texturized concentrate and grass hay.
• The horses were subjected to three different dietary treatments in a randomized, crossover design. This methodology has the advantage of controlling the individual characteristics of the horses, reducing variance and enhancing reliability of the results.
• In the first treatment, the horses’ normal diet was supplemented with no added Calmin (CON), in the second treatment it was supplemented with a 1x concentration of Calmin (MIN1x), and in the third treatment it was supplemented with two times the normal concentration of Calmin (MIN2x).
• Gastroscopy, a procedure using an endoscope to visualize the horse’s stomach and collect gastric juice samples, was performed prior to the addition of the treatments, as well as 2 hours and 4 hours after feeding. The pH of the gastric juice was then measured using a benchtop pH meter.

Research Findings

• The results showed a significant increase in gastric juice pH (indicating a decrease in acidity) from the starting time to the 2 and 4-hour marks after the horses were fed.
• More importantly, it was noted that 2 hours after feeding, the gastric juice pH in the MIN1x and MIN2x treatment groups was significantly higher (less acidic) than the control group.
• These results imply that the inclusion of Calmin in the horse’s meal increases the buffering capacity of the gastric juice post feeding, potentially helping to reduce the risk of ulceration.