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Veterinary research communications2006; 30(6); 623-635; doi: 10.1007/s11259-006-3346-9

The effect of aerosolized and intravenously administered clenbuterol and aerosolized fluticasone propionate on horses challenged with Aspergillus fumigatus antigen.

Abstract: Beta-agonists have been shown to display anti-inflammatory properties in several experimental models. The aim of this study was to investigate the anti-inflammatory properties of clenbuterol (CB), administered either intravenously or by aerosol, in comparison with fluticasone propionate (FP) in recurrent airway obstruction (RAO)-susceptible horses. Eight horses, of which five were known to be susceptible to RAO, underwent an inhalation challenge with Aspergillus fumigatus (AF) antigen and were treated with CB intravenously, CB by aerosol, or FP by aerosol. Twenty-four hours after the challenge, bronchoalveolar lavage was performed, the total and differential cell counts were assessed, and cytokines were measured in isolated alveolar macrophages. After challenge with AF, RAO-susceptible horses showed an increase in total cell count, based on an increase in macrophages and lymphocytes, which was inhibited by treatment with intravenous CB, aerosolized CB and aerosolized FP. Neutrophil ratios were decreased when treated with aerosolized CB and FP. Expression of interleukin (IL)-1beta and IL -8 was significantly increased after AF challenge . Interleukin -1beta was significantly decreased following treatment with intravenous CB, aerosolized CB and aerosolized FP, whereas only FP decreased the expression of IL-8. These data suggest that the anti-inflammatory property of CB provide new opportunities in the therapeutic intervention of early inflammation in RAO.
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Publication Date: 2006-07-14 PubMed ID: 16838204DOI: 10.1007/s11259-006-3346-9Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the effects of clenbuterol administered through inhalation or intravenously, comparing it with fluticasone propionate, in horses vulnerable to recurrent airway obstruction (RAO), with Aspergillus fumigatus as the eliciting factor.

Research Purpose and Method

  • This study was aimed at investigating the anti-inflammatory properties of clenbuterol, an adrenergic(beta-agonist) drug, in comparison to fluticasone propionate, a corticosteroid, in horses that are susceptible to recurrent airway obstruction (RAO).
  • A group of eight horses was chosen for the experiment, five of which were already known to be susceptible to recurrent airway obstruction.
  • An inhalation challenge with Aspergillus fumigatus, a commonplace fungus, was set up for all the horses.
  • The horses were subsequently treated with either clenbuterol via intravenous injection or aerosol inhalation, or fluticasone propionate via aerosol inhalation.

Research Procedure and Results

  • 24 hours into the treatment, bronchoalveolar lavage – a procedure where bronchioles and parts of lungs are washed and the fluid(Goal: examine the inflammatory response) is examined – was conducted.
  • Total and differential cell count analysis was conducted, along with an assessment of cytokines (cell-signalling protein)
  • Upon inhalation challenge with the Aspergillus fumigatus (AF) antigen, there was a noticeable increase in total cell count in the RAO-susceptible horses. This notably included an increase in macrophages and lymphocytes, and it was observed that this increase can be offset through treatment with clenbuterol, either via the intravenous route or aerosol, or fluticasone propionate.
  • The ratios of neutrophils, a type of white blood cell, were found to be decreased in samples treated with aerosol clenbuterol and fluticasone propionate
  • The expression of interleukin (IL)-1beta and IL -8, members of cytokines family, increased significantly after the AF challenge. Intravenous or aerosolized clenbuterol and fluticasone propionate were found to significantly decrease the expression of IL-1beta. However, it was only fluticasone propionate that reduced IL-8 expression.

Conclusion

  • The study concludes that the anti-inflammatory property of clenbuterol, particularly its ability to decrease the expression of IL-1beta, presents a potential for additional therapeutic intervention in early inflammation in recurrent airway obstruction in horses.

Cite This Article

APA
Laan TT, Bull S, van Nieuwstadt RA, Fink-Gremmels J. (2006). The effect of aerosolized and intravenously administered clenbuterol and aerosolized fluticasone propionate on horses challenged with Aspergillus fumigatus antigen. Vet Res Commun, 30(6), 623-635. https://doi.org/10.1007/s11259-006-3346-9

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 30
Issue: 6
Pages: 623-635

Researcher Affiliations

Laan, T T J M
  • Department of Equine Sciences, Internal Medicine Section, Utrecht University, Utrecht, The Netherlands. t.laan@vet.uu.nl
Bull, S
    van Nieuwstadt, R A
      Fink-Gremmels, J

        MeSH Terms

        • Administration, Inhalation
        • Androstadienes / administration & dosage
        • Androstadienes / therapeutic use
        • Animals
        • Antigens, Fungal / therapeutic use
        • Aspergillus fumigatus / immunology
        • Aspergillus fumigatus / pathogenicity
        • Bronchoalveolar Lavage Fluid / cytology
        • Bronchodilator Agents / administration & dosage
        • Bronchodilator Agents / therapeutic use
        • Clenbuterol / administration & dosage
        • Clenbuterol / therapeutic use
        • Cytokines / metabolism
        • Female
        • Fluticasone
        • Horse Diseases / drug therapy
        • Horse Diseases / immunology
        • Horses
        • Injections, Intravenous / veterinary
        • Lung Diseases, Obstructive / drug therapy
        • Lung Diseases, Obstructive / immunology
        • Lung Diseases, Obstructive / veterinary
        • Macrophages, Alveolar / metabolism
        • Random Allocation
        • Treatment Outcome

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        Citations

        This article has been cited 2 times.
        1. Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.. Animals (Basel) 2022 Mar 16;12(6).
          doi: 10.3390/ani12060744pubmed: 35327141google scholar: lookup
        2. Mainguy-Seers S, Lavoie JP. Glucocorticoid treatment in horses with asthma: A narrative review.. J Vet Intern Med 2021 Jul;35(4):2045-2057.
          doi: 10.1111/jvim.16189pubmed: 34085342google scholar: lookup
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