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Home / Equine Research Bank / Front Vet Sci / The Effect of Intense Exercise on Equine Serum Proteoglycan-...
Frontiers in veterinary science2020; 7; 599287; doi: 10.3389/fvets.2020.599287

The Effect of Intense Exercise on Equine Serum Proteoglycan-4/Lubricin.

Abstract: Local biological and biomechanical-stimuli modulate proteoglycan-4 secretion within synovial joints. For the horse, changes to proteoglycan-4 concentration and function are notable in acute joint injury and osteoarthritis. Proteoglycan-4 (also known as Lubricin) is present in the blood, however the effect of exercise on equine serum levels is unknown. The overall objective of this study was, therefore, to investigate the effect of intense exercise on serum proteoglycan-4 in thoroughbred horses. Samples of blood were taken from thoroughbreds ( = 12) during a chuckwagon racing event (Alberta, Canada). The chuckwagon race is a sprint racing event where teams of horses pull a combined 1,325 lbs (601 kg) of wagon and driver around a 5/8th mile (1 km) of dirt track, racing at full gallop to the finish. Blood samples were collected 30-min before the race start, and several timepoints post-race: 5-min, 90-min, 3-h, 12-h, and 23-h. Proteoglycan-4 concentrations in serum were quantified by enzyme-linked-immunosorbent-assay using recombinant-human proteoglycan-4 standards and anti-proteoglycan-4 mAb 9G3. The molecular weight of immunoreactive proteoglycan-4 in serum was assessed by western blot. Proteoglyan-4 in serum demonstrated the expected high MW immunoreactivity to mAb 9G3, consistent with that of full length PRG4. Serum proteoglycan-4 decreased five-minutes post-race from baseline concentration (0.815 ± 0.175 to 0.466 ± 0.090 μg/mL, μ ± SEM, < 0.01). The concentration of serum proteoglycan-4 in horses decreased significantly five min post-exercise. A potential explanation for this finding could be increased proteoglycan-4 clearance from the circulation. Further investigations could extend to complete the detailed characterization of proteoglycan-4 structure and its potential function within the blood as it relates to joint health and exercise.
Copyright © 2020 Matheson, Regmi, Jay, Schmidt and Scott.
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Publication Date: 2020-12-16 PubMed ID: 33392293PubMed Central: PMC7772952DOI: 10.3389/fvets.2020.599287Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the impact of intense exercise on serum proteoglycan-4 (also known as Lubricin), a substance present in the blood of thoroughbred horses, by examining the changes in the concentration of this substance before and after a racing event.

Objective of the Research

  • The main goal of this study was to investigate the effect of intense exercise on the concentration of serum proteoglycan-4 in thoroughbred horses.
  • The researchers focused specifically on how the levels of this substance vary within a set period encompassing the pre-race timeframe and various post-race timepoints: 5-minutes, 90-minutes, 3-hours, 12-hours, and 23-hours after the race.

Methodology

  • Using 12 thoroughbred horses during a chuckwagon racing event in Alberta, Canada, the researchers took blood samples at the time points indicated above.
  • This racing event involved horses pulling a very heavy load of wagon and driver across a fairly short dirt track at a full gallop, representing a context of intense physical exertion.
  • The researchers used an enzyme-linked-immunosorbent-assay to quantify the proteoglycan-4 concentrations, using recombinant-human proteoglycan-4 standards and an anti-proteoglycan-4 mAb 9G3.
  • A Western blot test was used to assess the molecular weight of the immunoreactive proteoglycan-4 present in the serum samples.

Results and Findings

  • The concentration of proteoglycan-4 in the serum samples taken five minutes post-race showed a significant decrease from baseline level (prior to the race).
  • The fall in proteoglycan-4 level is thought to possibly be due to heightened clearance of the substance from the horses’ bloodstream following this intensive exercise activity.

Implications and Future Research

  • The findings of this study present a potential aspect of joint health and exercise in horses, opening the door for further exploration and research into the importance of proteoglycan-4 and its role within the blood system in relation to joint health.
  • Future studies could provide more detailed characterizations of proteoglycan-4’s structure as well as investigate its specific function or handling in the bloodstream as it pertains to exercise and joint health in horses.

Cite This Article

APA
Matheson A, Regmi SC, Jay GD, Schmidt TA, Scott WM. (2020). The Effect of Intense Exercise on Equine Serum Proteoglycan-4/Lubricin. Front Vet Sci, 7, 599287. https://doi.org/10.3389/fvets.2020.599287

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 7
Pages: 599287
PII: 599287

Researcher Affiliations

Matheson, Austyn
  • Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.
Regmi, Suresh C
  • Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.
Jay, Gregory D
  • Department of Emergency Medicine, Warren Alpert Medical School & School of Engineering, Brown University, Providence, RI, United States.
Schmidt, Tannin A
  • Biomedical Engineering Department, University of Connecticut Health Center, Farmington, CT, United States.
Scott, W Michael
  • Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.
  • Department of Veterinary Clinical and Diagnostic Sciences, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.

Conflict of Interest Statement

TS and GJ own equity in Lμbris BioPharma and have licensed patents related to the use of rhPRG4. TS also consults for Lμbris BioPharma. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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