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Home / Equine Research Bank / Vet Sci / The Equine Dental Pulp: Histomorphometric Analysis of the Eq...
Veterinary sciences2022; 9(6); 261; doi: 10.3390/vetsci9060261

The Equine Dental Pulp: Histomorphometric Analysis of the Equine Dental Pulp in Incisors and Cheek Teeth.

Abstract: To maintain a healthy and functional status, equine hypsodont teeth have to produce lifelong large amounts of subocclusal dentin to prevent occlusal pulp exposure, which is caused by occlusal wear. To examine the cyto- and histological components that guarantee the lifelong high productivity of equine pulp, a limited number of ten incisors and ten cheek teeth from seven adult horses (aged 5 to 24 years) and five foals were sampled for preliminary histomorphometric and histomorphological evaluations. Independently of age, the equine dental pulp featured constant layers of predentin and odontoblastic cells, as well as soft connective tissue, composed of a cellular fibrous matrix, in which blood vessels and nerve fibers were embedded. As a result of the progressive deposition of newly formed dentin, the layer of dentin became thicker with age, and the size of the pulp chamber decreased. In contrast to the brachydont teeth, the morphological characteristics of the odontoblastic layer and the width of the predentin layer did not change with age. Therefore, it is assumed that the equine pulp tissue retained their juvenile status, which explains its unchanged ability to produce high amounts of subocclusal dentin. These preliminary, but clinically significant, findings are worthy of further investigation in order to identify strategies for equine-specific endodontic therapies.
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Publication Date: 2022-05-30 PubMed ID: 35737313PubMed Central: PMC9227316DOI: 10.3390/vetsci9060261Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the structure of equine teeth, specifically the layers of predentin and odontoblastic cells in the dental pulp, and their ability to constantly produce subocclusal dentin throughout a horse’s life. This continuous growth prevents pulp exposure caused by wear, which maintains the health and function of the teeth.

Method and Sample

  • The researchers sampled incisors and cheek teeth from seven adult horses aged between 5 to 24 years as well as from five foals.
  • This sample size was intended to be indicative but not exhaustive, providing preliminary data for future larger studies.
  • They performed histomorphometric and histomorphological evaluations to analyze the structural components of the equine dental pulp.

Findings

  • Independent of the age of the horse, the dental pulp was found to have constant layers of predentin and odontoblastic cells, along with soft connective tissue. This tissue was made up of a cellular fibrous matrix, with blood vessels and nerve fibers embedded.
  • As a result of the continuous deposition of newly formed dentin, the layer of dentin became thicker as the horse aged and the size of the pulp chamber decreased over time.
  • In contrast to the teeth structure in brachydont (or short-crowned) animals, the morphological characteristics of the odontoblastic layer and the width of the predentin layer in hypsodont (or long-crowned) horses remained constant throughout their lives.

Implications of Research

  • The study indicates that the equine pulp tissue retains its juvenile status, which explains its long-term ability to generate high amounts of subocclusal dentin.
  • This ultimately helps in maintaining the health and functionality of the horse’s teeth by preventing occlusal pulp exposure.
  • The researchers suggest that these findings could be clinically significant in identifying strategies for equine-specific endodontic therapies, meaning that this preliminary data could lead to better dental care for horses in the future.

Cite This Article

APA
Roßgardt J, Heilen LB, Büttner K, Dern-Wieloch J, Vogelsberg J, Staszyk C. (2022). The Equine Dental Pulp: Histomorphometric Analysis of the Equine Dental Pulp in Incisors and Cheek Teeth. Vet Sci, 9(6), 261. https://doi.org/10.3390/vetsci9060261

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 9
Issue: 6
PII: 261

Researcher Affiliations

Roßgardt, Jessica
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35390 Giessen, Germany.
Heilen, Laura Beate
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35390 Giessen, Germany.
Büttner, Kathrin
  • Unit for Biomathematics and Data Processing, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 95, 35390 Giessen, Germany.
Dern-Wieloch, Jutta
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35390 Giessen, Germany.
Vogelsberg, Jörg
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35390 Giessen, Germany.
Staszyk, Carsten
  • Institute of Veterinary-Anatomy, -Histology and -Embryology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Frankfurter Strasse 98, 35390 Giessen, Germany.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 46 references
  1. Staszyk C. Anatomie. In: Vogt C., editor. Lehrbuch der Zahnheilkunde beim Pferd. Schattauer; Stuttgart, Germany: 2011. pp. 1–29.
  2. Thomé H. Zähne und Gebiss. In: Nickel R., Schummer A., Seiferle E., editors. Lehrbuch der Anatomie der Haustiere: Band 2: Eingeweide. Parey; Berlin, Germany: 2004. pp. 79–102.
  3. Kilic S, Dixon PM, Kempson SA. A light microscopic and ultrastructural examination of calcified dental tissues of horses: 3. Dentine.. Equine Vet J 1997 May;29(3):206-12.
    doi: 10.1111/j.2042-3306.1997.tb01670.xpubmed: 9234013google scholar: lookup
  4. Dixon P.M. The Gross, Histological, and Ultrastructural Anatomy of Equine Teeth and Their Relationship to Disease. Proceedings of the Annual Convention of the AAEP; Orlando, FL, USA. 4–8 December 2002; pp. 421–437.
  5. Kilic S, Dixon PM, Kempson SA. A light microscopic and ultrastructural examination of calcified dental tissues of horses: 1. The occlusal surface and enamel thickness.. Equine Vet J 1997 May;29(3):190-7.
    doi: 10.1111/j.2042-3306.1997.tb01668.xpubmed: 9234011google scholar: lookup
  6. Dacre IT, Shaw DJ, Dixon PM. Pathological studies of cheek teeth apical infections in the horse: 3. Quantitative measurements of dentine in apically infected cheek teeth.. Vet J 2008 Dec;178(3):333-40.
    doi: 10.1016/j.tvjl.2008.09.014pubmed: 18977157google scholar: lookup
  7. Du Toit N, Kempson SA, Dixon PM. Donkey dental anatomy. Part 2: Histological and scanning electron microscopic examinations.. Vet J 2008 Jun;176(3):345-53.
    doi: 10.1016/j.tvjl.2008.03.004pubmed: 18396075google scholar: lookup
  8. Dixon PM, du Toit N, Staszyk C. A fresh look at the anatomy and physiology of equine mastication.. Vet Clin North Am Equine Pract 2013 Aug;29(2):257-72, v.
    doi: 10.1016/j.cveq.2013.04.006pubmed: 23915660google scholar: lookup
  9. Dixon P.M. Zahnanatomie. In: Baker G.J., Easley J., editors. Zahnheilkunde in der Pferdepraxis. 2nd ed. Schlütersche; Hannover, Germany: 2007. pp. 27–52.
  10. White C, Dixon PM. A study of the thickness of cheek teeth subocclusal secondary dentine in horses of different ages.. Equine Vet J 2010 Mar;42(2):119-23.
    doi: 10.2746/042516409X475409pubmed: 20156246google scholar: lookup
  11. Liebich H.-G. Funktionelle Histologie der Haussäugetiere und Vögel: Lehrbuch und Farbatlas für Studium und Praxis. 5th ed. Schattauer; Stuttgart, Germany: 2010.
  12. König H.E., Sótonyi P., Schöpper H., Liebich H.-G. Kauapparat. In: König H.E., Liebich H.-G., editors. Anatomie der Haustiere: Lehrbuch und Farbatlas für Studium und Praxis. Thieme; Stuttgart, Germany: 2019. 7. Aktualisierte und Erweiterte Auflage.
  13. Schroeder H.E. Orale Strukturbiologie: Entwicklungsgeschichte, Struktur und Funktion Normaler Hart- und Weichgewebe der Mundhöhle und des Kiefergelenks. 5th ed. Thieme; Stuttgart, Germany: 2000.
  14. Nanci A. Ten Cate’s Oral Histology: Development, Structure, and Function. 8th ed. Elsevier Mosby; St. Louis, MI, USA: 2014.
  15. Klugh D.O. Principles of Equine Dentistry. Manson Publishing; London, UK: 2010.
  16. Berkovitz B.K.B., Holland G.R., Moxham B.J. Oral Anatomy, Histology and Embryology. 5th ed. Elsevier; Edinburgh, UK: London, UK: New York, NY, USA: Oxford, UK: Philadelphia, PA, USA: St. Louis, MO, USA: Sydney, Australia: Toronto, ON, Canada: 2018.
  17. Goldberg M. The Dental Pulp: Biology, Pathology, and Regenerative Therapies. Springer; Berlin/Heidelberg, Germany: 2014. Pulp Anatomy and Characterization of Pulp Cells; pp. 13–33.
  18. Kirkland KD, Baker GJ, Manfra Marretta S, Eurell JA, Losonsky JM. Effects of aging on the endodontic system, reserve crown, and roots of equine mandibular cheek teeth.. Am J Vet Res 1996 Jan;57(1):31-8.
    pubmed: 8720234
  19. Lundström T., Wattle O. Description of a technique for orthograde endodontic treatment of equine cheek teeth with apical infections. Equine Vet. Educ. 2016;28:641–652.
    doi: 10.1111/eve.12540google scholar: lookup
  20. Casey M. A new understanding of oral and dental pathology of the equine cheek teeth.. Vet Clin North Am Equine Pract 2013 Aug;29(2):301-24, v.
    doi: 10.1016/j.cveq.2013.04.010pubmed: 23915662google scholar: lookup
  21. Dixon PM, Dacre I. A review of equine dental disorders.. Vet J 2005 Mar;169(2):165-87.
    doi: 10.1016/j.tvjl.2004.03.022pubmed: 15727909google scholar: lookup
  22. Muylle S, Simoens P, Lauwers H. Age-related morphometry of equine incisors.. Zentralbl Veterinarmed A 1999 Dec;46(10):633-43.
    doi: 10.1046/j.1439-0442.1999.00261.xpubmed: 10638301google scholar: lookup
  23. Murray PE, Stanley HR, Matthews JB, Sloan AJ, Smith AJ. Age-related odontometric changes of human teeth.. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002 Apr;93(4):474-82.
    doi: 10.1067/moe.2002.120974pubmed: 12029288google scholar: lookup
  24. Staszyk C., Suske A., Pöschke A. Equine dental and periodontal anatomy: A tutorial review. Equine Vet. Educ. 2015;27:474–481.
    doi: 10.1111/eve.12317google scholar: lookup
  25. Kopke S, Angrisani N, Staszyk C. The dental cavities of equine cheek teeth: three-dimensional reconstructions based on high resolution micro-computed tomography.. BMC Vet Res 2012 Sep 25;8:173.
    doi: 10.1186/1746-6148-8-173pmc: PMC3514392pubmed: 23006500google scholar: lookup
  26. Illenberger N., Brehm W., Ludewig E., Gerlach K. Darstellung altersabhängiger Veränderungen der Zahnpulpen ausgewählter Oberkieferbackenzähne des Pferdes mittels Magnetresonanztomographie. Pferdeheilkunde 2013;29:183–188.
  27. Englisch LM, Rott P, Lüpke M, Seifert H, Staszyk C. Anatomy of equine incisors: Pulp horns and subocclusal dentine thickness.. Equine Vet J 2018 Nov;50(6):854-860.
    doi: 10.1111/evj.12841pubmed: 29654603google scholar: lookup
  28. Gasse H., Westenberger E., Staszyk C. The endodontic system of equine cheek teeth: A reexamination of pulp horns and root canals in view of age-related physiological differences. PHK 2004;20:13–18.
    doi: 10.21836/PEM20040102google scholar: lookup
  29. Floyd MR. The modified Triadan system: nomenclature for veterinary dentistry.. J Vet Dent 1991 Dec;8(4):18-9.
    doi: 10.1177/089875649100800402pubmed: 1815632google scholar: lookup
  30. Dacre I, Kempson S, Dixon PM. Pathological studies of cheek teeth apical infections in the horse: 5. Aetiopathological findings in 57 apically infected maxillary cheek teeth and histological and ultrastructural findings.. Vet J 2008 Dec;178(3):352-63.
    doi: 10.1016/j.tvjl.2008.09.024pubmed: 19022689google scholar: lookup
  31. Schoppe C, Hellige M, Rohn K, Ohnesorge B, Bienert-Zeit A. Comparison of computed tomography and high-field (3.0 T) magnetic resonance imaging of age-related variances in selected equine maxillary cheek teeth and adjacent tissues.. BMC Vet Res 2017 Sep 6;13(1):280.
    doi: 10.1186/s12917-017-1200-7pmc: PMC5585999pubmed: 28874149google scholar: lookup
  32. Windley Z, Weller R, Tremaine WH, Perkins JD. Two- and three-dimensional computed tomographic anatomy of the enamel, infundibulae and pulp of 126 equine cheek teeth. Part 1: Findings in teeth without macroscopic occlusal or computed tomographic lesions.. Equine Vet J 2009 May;41(5):433-40.
    doi: 10.2746/042516409X390214pubmed: 19642402google scholar: lookup
  33. Sue M, Oda T, Sasaki Y, Ogura I. Age-related changes in the pulp chamber of maxillary and mandibular molars on cone-beam computed tomography images.. Oral Radiol 2018 Sep;34(3):219-223.
    doi: 10.1007/s11282-017-0300-1pubmed: 30484030google scholar: lookup
  34. Maddalone M, Citterio C, Pellegatta A, Gagliani M, Karanxha L, Del Fabbro M. Cone-beam computed tomography accuracy in pulp chamber size evaluation: An ex vivo study.. Aust Endod J 2020 Apr;46(1):88-93.
    doi: 10.1111/aej.12378pubmed: 31617650google scholar: lookup
  35. Baratt R.M. Challenges in developing valid techniques for equine endodontic treatment of apically infected cheek teeth. Equine Vet. Educ. 2016;28:609–613.
    doi: 10.1111/eve.12583google scholar: lookup
  36. Mazzi-Chaves J.F., Camargo R.V., Borges A.F., Silva R.G., Pauwels R., Silva-Sousa Y.T.C., Sousa-Neto M.D. Cone-Beam Computed Tomography in Endodontics—State of the Art. Curr. Oral Health Rep. 2021;8:9–22.
    doi: 10.1007/s40496-021-00292-8google scholar: lookup
  37. Maeda H. Aging and Senescence of Dental Pulp and Hard Tissues of the Tooth.. Front Cell Dev Biol 2020;8:605996.
    doi: 10.3389/fcell.2020.605996pmc: PMC7734349pubmed: 33330507google scholar: lookup
  38. Couve E, Schmachtenberg O. Autophagic activity and aging in human odontoblasts.. J Dent Res 2011 Apr;90(4):523-8.
    doi: 10.1177/0022034510393347pubmed: 21212314google scholar: lookup
  39. Burke FM, Samarawickrama DY. Progressive changes in the pulpo-dentinal complex and their clinical consequences.. Gerodontology 1995 Dec;12(12):57-66.
    doi: 10.1111/j.1741-2358.1995.tb00132.xpubmed: 9084291google scholar: lookup
  40. Baker A, Karpagaselvi K, Kumaraswamy J, Ranjini MR, Gowher J. Role of dental pulp in age estimation: A quantitative and morphometric study.. J Forensic Dent Sci 2019 May-Aug;11(2):95-102.
    doi: 10.4103/jfo.jfds_57_19pmc: PMC7006301pubmed: 32082045google scholar: lookup
  41. Daud S, Nambiar P, Hossain MZ, Rahman MR, Bakri MM. Changes in cell density and morphology of selected cells of the ageing human dental pulp.. Gerodontology 2016 Sep;33(3):315-21.
    doi: 10.1111/ger.12154pubmed: 25266855google scholar: lookup
  42. Couve E, Osorio R, Schmachtenberg O. The amazing odontoblast: activity, autophagy, and aging.. J Dent Res 2013 Sep;92(9):765-72.
    doi: 10.1177/0022034513495874pubmed: 23803461google scholar: lookup
  43. Couve E. Ultrastructural changes during the life cycle of human odontoblasts.. Arch Oral Biol 1986;31(10):643-51.
    doi: 10.1016/0003-9969(86)90093-2pubmed: 3477208google scholar: lookup
  44. Steiniger B., Schwarzbach H., Stachniss V. Mikroskopische Anatomie der Zähne und des Parodonts. Thieme; Stuttgart, Germany: 2010.
  45. Basandi PS, Madammal RM, Adi RP, Donoghue M, Nayak S, Manickam S. Predentin thickness analysis in developing and developed permanent teeth.. J Nat Sci Biol Med 2015 Jul-Dec;6(2):310-3.
    doi: 10.4103/0976-9668.159987pmc: PMC4518399pubmed: 26283819google scholar: lookup
  46. Couve E. Changes in the predentin thickness and mineralization front configuration in developing human premolars.. Acta Anat (Basel) 1987;130(4):324-8.
    doi: 10.1159/000146464pubmed: 3434185google scholar: lookup

Citations

This article has been cited 5 times.
  1. Heilen LB, Roßgardt J, Dern-Wieloch J, Vogelsberg J, Staszyk C. Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament. Front Vet Sci 2023;10:1116671.
    doi: 10.3389/fvets.2023.1116671pubmed: 36968463google scholar: lookup
  2. Roßgardt J, Heilen LB, Büttner K, Dern-Wieloch J, Vogelsberg J, Staszyk C. The Equine Dental Pulp: Analysis of the Stratigraphic Arrangement of the Equine Dental Pulp in Incisors and Cheek Teeth. Vet Sci 2022 Oct 30;9(11).
    doi: 10.3390/vetsci9110602pubmed: 36356079google scholar: lookup
  3. Heilen LB, Roßgardt J, Dern-Wieloch J, Vogelsberg J, Staszyk C. Odontoblasts in Equine Hypsodont Teeth-How They Cope with Permanent Occlusal Wear. Animals (Basel) 2026 Jan 22;16(2).
    doi: 10.3390/ani16020341pubmed: 41594529google scholar: lookup
  4. Korsós SA, Staszyk C, Boone M, Josipovic I, Vogelsberg J, Vlaminck L. Micro-CT and histological examination of accessory canals in 34 equine cheek teeth. Front Vet Sci 2024;11:1396871.
    doi: 10.3389/fvets.2024.1396871pubmed: 38659446google scholar: lookup
  5. Hertel S, Basche S, Schmidt V, Staszyk C, Hannig C, Sterzenbach T, Hannig M. Erosion behaviour of human, bovine and equine dental hard tissues. Sci Rep 2023 Nov 10;13(1):19617.
    doi: 10.1038/s41598-023-46759-9pubmed: 37949920google scholar: lookup
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