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Reproduction in domestic animals = Zuchthygiene2003; 38(4); 305-311; doi: 10.1046/j.1439-0531.2003.00451.x

The predictive value of semen analysis in the evaluation of stallion fertility.

Abstract: Pregnancy rates in managed horse populations depend on the innate fertility of the mares and stallions involved and on the quality of breeding management. Of course, because a single stallion usually mates many mares, stallion fertility is a critical factor in the overall success of a breeding program. Unfortunately, accurate evaluation of stallion fertility per se requires a large number of normal mares to be mated and is necessarily retrospective. Rather, the ideal is to predict fertility in advance of the stallion's breeding career, and this is currently attempted by way of a thorough physical examination and a routine analysis of semen quality. However, while such a 'breeding soundness examination' identifies stallions that clearly lack the capacity for adequate fertility, it is of limited use for predicting the level of fertility and fails to identify some seriously sub-fertile animals. Similarly, while various sperm function tests (e.g., sperm head morphometry, the hypoosmotic swelling test, glass wool-sephadex filtration, progesterone receptor exposure) have been shown to correlate fairly well with fertility in the field, most examine only a single or a narrow range of the attributes that a sperm must possess if it is to fertilize an oocyte in vivo, and are thus more useful for identifying specific causes of sub-fertility than for predicting the level of fertility. On the other hand, combining the results of the various sperm function tests does improve the reliability of fertility estimation and current research is therefore concentrated on identifying a range of tests that covers as many important sperm attributes as possible but that can be performed rapidly and cheaply. In this respect, flow-cytometry has proven to be an ideal tool because it allows the objective, rapid and simultaneous analysis of a number of properties in a large number of sperm. Moreover, stains are available for an increasing range of sperm characteristics including viability, capacitation and acrosome status, mitochondrial activity and chromatin integrity. Flow-cytometric analysis of sperm with appropriate probes thus offers considerable promise for the prediction of stallion fertility.
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Publication Date: 2003-07-31 PubMed ID: 12887569DOI: 10.1046/j.1439-0531.2003.00451.xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper explores methods of predicting stallion fertility, primarily focusing on semen analysis and noting that although current tests can identify fertility issues, they aren’t as effective at defining levels of fertility. The overall aim of the research is to develop a more reliable and cost-effective method of predicting fertility in stallions. Current research is now centered on identifying a range of tests that covers a multitude of important sperm attributes. The paper suggests that flow-cytometry, which allows rapid and concurrent analysis of multiple sperm properties, offers significant potential in predicting stallion fertility.

Understanding the Importance of Stallion Fertility Testing

  • In managed horse populations, the overall success rate of breeding programs primarily depends on the fertility of mares and stallions. Stallions are especially critical due to the higher number of potential mates.
  • The research highlights the necessity for a prospective rather than retrospective fertility evaluation. A proactive approach would allow breeding programs to assess the future reproductive potential of a stallion and not just reflect on past performance.

Current Methods of Fertility Estimation

  • At present, stallion fertility assessment relies on a thorough physical examination and routine semen analysis. This procedure, often called a ‘breeding soundness examination‘, can identify stallions with obvious fertility issues. However, these methods are less effective when it comes to determining the level of fertility and can overlook some sub-fertile animals.
  • Sperm function tests, like sperm head morphometry, the hypoosmotic swelling test, glass wool-sephadex filtration, and progesterone receptor exposure, have demonstrated reasonable correlations with field fertility. However, these tests usually focus on a single or narrow range of sperm attributes and are better at indicating specific sub-fertility causes rather than predicting fertility levels.

Improving Fertility Estimation

  • The researchers suggest that combining the results of various sperm function tests can improve fertility estimation reliability. The challenge, however, lies in identifying a suite of tests that can rapidly and inexpensively examine multiple vital sperm attributes.
  • Flow-cytometry emerges as a promising tool in this respect. This method allows the objective, quick, and simultaneous analysis of numerous sperm properties.
  • Flow-cytometry can examine a growing range of sperm features, including viability, capacitation and acrosome status, mitochondrial function, and chromatin integrity.
  • The paper concludes by emphasizing the significant potential of flow-cytometric analysis in predicting stallion fertility when it is used in conjunction with the appropriate probes.

Cite This Article

APA
Colenbrander B, Gadella BM, Stout TA. (2003). The predictive value of semen analysis in the evaluation of stallion fertility. Reprod Domest Anim, 38(4), 305-311. https://doi.org/10.1046/j.1439-0531.2003.00451.x

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 0936-6768
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 38
Issue: 4
Pages: 305-311

Researcher Affiliations

Colenbrander, B
  • Departments of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. b.colenbrander@vet.uu.nl
Gadella, B M
    Stout, T A E

      MeSH Terms

      • Animals
      • Female
      • Fertility
      • Flow Cytometry / standards
      • Horses / physiology
      • Male
      • Predictive Value of Tests
      • Pregnancy
      • Pregnancy Rate
      • Semen / cytology
      • Semen / physiology
      • Semen Preservation
      • Sperm Count
      • Sperm Motility

      References

      This article includes 64 references

      Citations

      This article has been cited 31 times.
      1. de Figueiredo MM, Bueno VC, Royes ICL, Mattos RC, Bastos HBA, Rechsteiner SF. Protamine1, 2 and Catsper1: sperm quality and fertility indicators in Stallions. Anim Reprod 2025;22(4):e20250040.
        doi: 10.1590/1984-3143-AR2025-0040pubmed: 41393904google scholar: lookup
      2. Najjar A, Dhaouadi A, Ezzar S, Benaoun B, Khaldi S. Sexual arousal intensity modulates copulatory behavior and semen quality in Arabian stallions: Effects of age, origin, and collection method. Vet World 2025 Sep;18(9):2615-2625.
        doi: 10.14202/vetworld.2025.2615-2625pubmed: 41113212google scholar: lookup
      3. Wen X, Bou G, He Q, Liu Q, Yi M, Ren H. Comprehensive Integrated Analyses of Proteins and Metabolites in Equine Seminal Plasma (Horses and Donkeys). Proteomes 2025 Jul 4;13(3).
        doi: 10.3390/proteomes13030033pubmed: 40700277google scholar: lookup
      4. Medica AJ, Gibb Z, Aitken RJ. Optimizing equine sperm quality: an alternative to single layer centrifugation for sperm isolation. Reprod Fertil 2024 Oct 1;5(4).
        doi: 10.1530/RAF-23-0081pubmed: 39437190google scholar: lookup
      5. Ren H, Wen X, He Q, Yi M, Dugarjaviin M, Bou G. Comparative Study on the Sperm Proteomes of Horses and Donkeys. Animals (Basel) 2024 Jul 31;14(15).
        doi: 10.3390/ani14152237pubmed: 39123763google scholar: lookup
      6. Dordas-Perpinyà M, Yánez-Ortiz I, Sergeant N, Mevel V, Catalán J, Bruyas JF, Miró J, Briand-Amirat L. ProAKAP4 as Indicator of Long-Lasting Motility Marker in Post-Thaw Conditions in Stallions. Animals (Basel) 2024 Apr 23;14(9).
        doi: 10.3390/ani14091264pubmed: 38731267google scholar: lookup
      7. Bueno VC, Bastos HBA, Centeno LA, Kretzmann NA, Mattos RC, Rechsteiner SF. PLCζ, WBP2NL and TNF-α expression in spermatozoa is associated with stallion fertility and seminal quality?. Anim Reprod 2024;21(1):e20230088.
        doi: 10.1590/1984-3143-AR2023-0088pubmed: 38628496google scholar: lookup
      8. Al-Kass Z, Morrell JM. Freezing Stallion Semen-What Do We Need to Focus on for the Future?. Vet Sci 2024 Feb 2;11(2).
        doi: 10.3390/vetsci11020065pubmed: 38393083google scholar: lookup
      9. Harris IT, Maddock C, Farnworth M, Nankervis K, Perrett J, Pyatt AZ, Blanchard RN. Temporal trends in equine sperm progressive motility: a systematic review and meta-regression. Reproduction 2023 Jun 1;165(6):M1-M10.
        doi: 10.1530/REP-22-0490pubmed: 37000597google scholar: lookup
      10. Dordas-Perpinyà M, Yanez-Ortiz I, Sergeant N, Mevel V, Bruyas JF, Catalán J, Delehedde M, Briand-Amirat L, Miró J. ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen-Thawed Horse Semen. Animals (Basel) 2022 Dec 4;12(23).
        doi: 10.3390/ani12233417pubmed: 36496938google scholar: lookup
      11. Boni R, Gallo A, Tosti E. Electrophysiology and Fluorescence Spectroscopy Approaches for Evaluating Gamete and Embryo Functionality in Animals and Humans. Biomolecules 2022 Nov 14;12(11).
        doi: 10.3390/biom12111685pubmed: 36421699google scholar: lookup
      12. Roach J, Arango Sabogal JC, Smith K, Foote A, Verheyen K, de Mestre AM. Multivariable analysis to determine risk factors associated with abortion in mares. Reprod Fertil 2022 Nov 1;3(4):301-12.
        doi: 10.1530/RAF-22-0087pubmed: 36374277google scholar: lookup
      13. Perrett J, Harris IT, Maddock C, Farnworth M, Pyatt AZ, Sumner RN. Systematic Analysis of Breed, Methodological, and Geographical Impact on Equine Sperm Progressive Motility. Animals (Basel) 2021 Oct 29;11(11).
        doi: 10.3390/ani11113088pubmed: 34827820google scholar: lookup
      14. Umair M, Henning H, Stout TAE, Claes A. A Modified Flotation Density Gradient Centrifugation Technique Improves the Semen Quality of Stallions with a High DNA Fragmentation Index. Animals (Basel) 2021 Jul 1;11(7).
        doi: 10.3390/ani11071973pubmed: 34359101google scholar: lookup
      15. Tanga BM, Qamar AY, Raza S, Bang S, Fang X, Yoon K, Cho J. Semen evaluation: methodological advancements in sperm quality-specific fertility assessment - A review. Anim Biosci 2021 Aug;34(8):1253-1270.
        doi: 10.5713/ab.21.0072pubmed: 33902175google scholar: lookup
      16. Arif AA, Maulana T, Kaiin EM, Purwantara B, Arifiantini RI, Memili E. Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen. Vet World 2020 Nov;13(11):2422-2428.
        doi: 10.14202/vetworld.2020.2422-2428pubmed: 33363336google scholar: lookup
      17. Ortiz I, Dorado J, Morrell JM, Diaz-Jimenez MA, Pereira B, Consuegra C, Hidalgo M. Comparison of sperm selection techniques in donkeys: motile subpopulations from a practical point of view. Anim Reprod 2019 Oct 23;16(2):282-289.
        doi: 10.21451/1984-3143-AR2018-0133pubmed: 33224288google scholar: lookup
      18. Gacem S, Catalán J, Valverde A, Soler C, Miró J. Optimization of CASA-Mot Analysis of Donkey Sperm: Optimum Frame Rate and Values of Kinematic Variables for Different Counting Chamber and Fields. Animals (Basel) 2020 Oct 29;10(11).
        doi: 10.3390/ani10111993pubmed: 33138237google scholar: lookup
      19. Cappai MG, Taras A, Cossu I, Cherchi R, Dimauro C, Accioni F, Boatto G, Deroma M, Spanu E, Gatta D, Dall'Aglio C, Pinna W. Effects of Dietary Zn/Se and α-Tocopherol Supplementation on Metabolic Milieu, Haemogram and Semen Traits of Breeding Stallions. Biol Trace Elem Res 2021 Sep;199(9):3287-3296.
        doi: 10.1007/s12011-020-02447-7pubmed: 33098077google scholar: lookup
      20. Ajuogu PK, Ere R, Nodu MB, Nwachukwu CU, Mgbere OO. The influence of graded levels of Cyathula prostrata (Linn.) Blume on semen quality characteristics of adult New Zealand white bucks. Transl Anim Sci 2020 Apr;4(2):txaa060.
        doi: 10.1093/tas/txaa060pubmed: 32705055google scholar: lookup
      21. Kowalczyk A, Czerniawska-Piątkowska E, Kuczaj M. Factors Influencing the Popularity of Artificial Insemination of Mares in Europe. Animals (Basel) 2019 Jul 19;9(7).
        doi: 10.3390/ani9070460pubmed: 31331026google scholar: lookup
      22. García-Molina A, Valverde A, Bompart D, Caldeira C, Vendrell A, Soler C. Updating semen analysis: a subpopulation approach. Asian J Androl 2020 Jan-Feb;22(1):118-119.
        doi: 10.4103/aja.aja_33_19pubmed: 31169137google scholar: lookup
      23. Gamboa S, Quaresma A, Castro F, Bravo P, Rebordão MR, Oom MDM, Rocha A. In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™. Saudi J Biol Sci 2017 Nov;24(7):1489-1496.
        doi: 10.1016/j.sjbs.2016.01.030pubmed: 30294217google scholar: lookup
      24. Jarosz Ł, Grądzki Z, Kalinowski M, Laskowska E. Quality of fresh and chilled-stored raccoon dog semen and its impact on artificial insemination efficiency. BMC Vet Res 2016 Oct 10;12(1):224.
        doi: 10.1186/s12917-016-0858-6pubmed: 27724853google scholar: lookup
      25. Gibb Z, Aitken RJ. The Impact of Sperm Metabolism during In Vitro Storage: The Stallion as a Model. Biomed Res Int 2016;2016:9380609.
        doi: 10.1155/2016/9380609pubmed: 26881234google scholar: lookup
      26. Das PJ, McCarthy F, Vishnoi M, Paria N, Gresham C, Li G, Kachroo P, Sudderth AK, Teague S, Love CC, Varner DD, Chowdhary BP, Raudsepp T. Stallion sperm transcriptome comprises functionally coherent coding and regulatory RNAs as revealed by microarray analysis and RNA-seq. PLoS One 2013;8(2):e56535.
        doi: 10.1371/journal.pone.0056535pubmed: 23409192google scholar: lookup
      27. Gutiérrez-Cepeda L, Fernández A, Crespo F, Ramírez MÁ, Gosálvez J, Serres C. The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA. Acta Vet Scand 2012 Dec 5;54(1):72.
        doi: 10.1186/1751-0147-54-72pubmed: 23217215google scholar: lookup
      28. Carreira JT, Mingoti GZ, Rodrigues LH, Silva C, Perri SH, Koivisto MB. Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa. Acta Vet Scand 2012 Jan 12;54(1):1.
        doi: 10.1186/1751-0147-54-1pubmed: 22240071google scholar: lookup
      29. Morrell JM, Johannisson A, Juntilla L, Rytty K, Bäckgren L, Dalin AM, Rodriguez-Martinez H. Stallion Sperm Viability, as Measured by the Nucleocounter SP-100, Is Affected by Extender and Enhanced by Single Layer Centrifugation. Vet Med Int 2010;2010:659862.
        doi: 10.4061/2010/659862pubmed: 20445788google scholar: lookup
      30. Morrell JM, Johannisson A, Dalin AM, Hammar L, Sandebert T, Rodriguez-Martinez H. Sperm morphology and chromatin integrity in Swedish warmblood stallions and their relationship to pregnancy rates. Acta Vet Scand 2008 Jan 7;50(1):2.
        doi: 10.1186/1751-0147-50-2pubmed: 18179691google scholar: lookup
      31. Kuisma P, Andersson M, Koskinen E, Katila T. Fertility of frozen-thawed stallion semen cannot be predicted by the currently used laboratory methods. Acta Vet Scand 2006 Aug 17;48(1):14.
        doi: 10.1186/1751-0147-48-14pubmed: 16987393google scholar: lookup
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