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TheScientificWorldJournal2013; 2013; 734923; doi: 10.1155/2013/734923

The segmental morphometric properties of the horse cervical spinal cord: a study of cadaver.

Abstract: Although the cervical spinal cord (CSC) of the horse has particular importance in diseases of CNS, there is very little information about its segmental morphometry. The objective of the present study was to determine the morphometric features of the CSC segments in the horse and possible relationships among the morphometric features. The segmented CSC from five mature animals was used. Length, weight, diameter, and volume measurements of the segments were performed macroscopically. Lengths and diameters of segments were measured histologically, and area and volume measurements were performed using stereological methods. The length, weight, and volume of the CSC were 61.6±3.2 cm, 107.2±10.4 g, and 95.5±8.3 cm3, respectively. The length of the segments was increased from C1 to C3, while it decreased from C3 to C8. The gross section (GS), white matter (WM), grey matter (GM), dorsal horn (DH), and ventral horn (VH) had the largest cross-section areas at C8. The highest volume was found for the total segment and WM at C4, GM, DH, and VH at C7, and the central canal (CC) at C3. The data obtained not only contribute to the knowledge of the normal anatomy of the CSC but may also provide reference data for veterinary pathologists and clinicians.
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Publication Date: 2013-02-07 PubMed ID: 23476145PubMed Central: PMC3582170DOI: 10.1155/2013/734923Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about an investigation into the specific morphometric properties of the cervical spinal cord (CSC) in horses. The study sought to gather new insights on the relationships between these properties, and how this information can be useful for animal health.

Objective of the Study

The main aim of the study was to gain more detailed knowledge about the morphometric features of the horse’s cervical spinal cord, due particular importance it carries in central nervous system diseases. The findings from this research could provide significant insights for veterinary pathologists and clinicians.

  • The cervical spinal cord was obtained from five mature horses after their death.
  • Several properties such as length, weight, diameter, and volume of the segmented CSC were examined in detail.

Methodology

Certain experimental techniques were used during the study:

  • The measurements of lengths and diameters of the CSC segments were conducted using histology, a study of the microscopic structure of tissues.
  • The area and volume measurements of the parts of the cord were done using stereological methods, a set of mathematical techniques used for three-dimensional interpretation of two-dimensional data.

Results of the Study

Several significant findings emerged from the research:

  • The observed length, weight, and volume of the cervical spinal cord were found to be 61.6±3.2 cm, 107.2±10.4 g, and 95.5±8.3 cm3 respectively.
  • The length of the segments was discovered to increase from the first to the third cervical vertebrae (C1 to C3), and decrease from the third to the eighth (C3 to C8).
  • The largest cross-section areas for the gross section (GS), white matter (WM), grey matter (GM), dorsal horn (DH), and ventral horn (VH) were all found at the eight cervical vertebrae (C8).
  • The highest volume was discovered for total segment and white matter at the fourth cervical vertebrae (C4), grey matter, dorsal horn, and ventral horn at the seventh (C7), and the central canal (CC) at the third (C3).

Significance of the Study

The research provides valuable insights to the CSC’s normal anatomy and can serve as a useful reference for veterinary pathologists and clinicians dealing with equine neurology. The results might help in diagnosing and treating diseases related to the central nervous system in horses. These findings also extend the limited existing data about the morphometry of the horse’s cervical spinal cord.

Cite This Article

APA
Bahar S, Bolat D, Selcuk ML. (2013). The segmental morphometric properties of the horse cervical spinal cord: a study of cadaver. ScientificWorldJournal, 2013, 734923. https://doi.org/10.1155/2013/734923

Publication

TheScientificWorldJournal
ISSN: 1537-744X
NlmUniqueID: 101131163
Country: United States
Language: English
Volume: 2013
Pages: 734923
PII: 734923

Researcher Affiliations

Bahar, Sadullah
  • Department of Anatomy, College of Veterinary Medicine, University of Selcuk, Selcuklu, 42075 Konya, Turkey.
Bolat, Durmus
    Selcuk, Muhammet Lutfi

      MeSH Terms

      • Anatomy, Cross-Sectional
      • Animals
      • Brain / anatomy & histology
      • Cervical Vertebrae / anatomy & histology
      • Horses / anatomy & histology
      • Microscopy
      • Organ Size
      • Reproducibility of Results
      • Spinal Cord / anatomy & histology

      References

      This article includes 50 references
      1. Ko HY, Park JH, Shin YB, Baek SY. Gross quantitative measurements of spinal cord segments in human.. Spinal Cord 2004 Jan;42(1):35-40.
        pubmed: 14713942doi: 10.1038/sj.sc.3101538google scholar: lookup
      2. THOMAS CE, COMBS CM. SPINAL CORD SEGMENTS. B. GROSS STRUCTURE IN THE ADULT MONKEY.. Am J Anat 1965 Jan;116:205-16.
        pubmed: 14283282doi: 10.1002/aja.1001160110google scholar: lookup
      3. BRAUN A. [Segmental histology of the equine spinal cord].. Acta Anat Suppl (Basel) 1950;12:1-76.
        pubmed: 24538334
      4. Turgut M, Turkkani Tunc A, Aslan H, Yazici AC, Kaplan S. Effect of pinealectomy on the morphology of the chick cervical spinal cord: a stereological and histopathological study.. Brain Res 2007 Jan 19;1129(1):166-73.
        pubmed: 17157276doi: 10.1016/j.brainres.2006.10.029google scholar: lookup
      5. da Costa RC, Parent JM, Partlow G, Dobson H, Holmberg DL, Lamarre J. Morphologic and morphometric magnetic resonance imaging features of Doberman Pinschers with and without clinical signs of cervical spondylomyelopathy.. Am J Vet Res 2006 Sep;67(9):1601-12.
        pubmed: 16948609doi: 10.2460/ajvr.67.9.1601google scholar: lookup
      6. Fujiwara K, Yonenobu K, Hiroshima K, Ebara S, Yamashita K, Ono K. Morphometry of the cervical spinal cord and its relation to pathology in cases with compression myelopathy.. Spine (Phila Pa 1976) 1988 Nov;13(11):1212-6.
        pubmed: 3206280doi: 10.1097/00007632-198811000-00002google scholar: lookup
      7. Sherman JL, Nassaux PY, Citrin CM. Measurements of the normal cervical spinal cord on MR imaging.. AJNR Am J Neuroradiol 1990 Mar-Apr;11(2):369-72.
        pubmed: 2107721
      8. Mitchel CW. The MRI findings in horses with spinal cord ataxia.. 2009.
      9. van Uitert R, Bitter I, Butman JA. Semi-automatic spinal cord segmentation and quantification.. International Congress Series 2005;1281:224–229.
      10. Howard V, Reed MG. Unbiased Stereology: Three-Dimensional Measurement in Microscopy.. 2005.
      11. Mouton PR. Principles and Practices of Unbiased Stereology.. 2002.
      12. Mayhew TM, Mwamengele GL, Dantzer V. Comparative morphometry of the mammalian brain: estimates of cerebral volumes and cortical surface areas obtained from macroscopic slices.. J Anat 1990 Oct;172:191-200.
        pmc: PMC1257214pubmed: 2272902
      13. Zarow C, Kim TS, Singh M, Chui HC. A standardized method for brain-cutting suitable for both stereology and MRI-brain co-registration.. J Neurosci Methods 2004 Oct 30;139(2):209-15.
        pubmed: 15488234doi: 10.1016/j.jneumeth.2004.04.034google scholar: lookup
      14. Bjugn R, Gundersen HJ. Estimate of the total number of neurons and glial and endothelial cells in the rat spinal cord by means of the optical disector.. J Comp Neurol 1993 Feb 15;328(3):406-14.
        pubmed: 8440788doi: 10.1002/cne.903280307google scholar: lookup
      15. Bjugn R, Bøe R, Haugland HK. A stereological study of the ependyma of the mouse spinal cord. With a comparative note on the choroid plexus ependyma.. J Anat 1989 Oct;166:171-8.
        pmc: PMC1256750pubmed: 2621136
      16. Laugier C, Tapprest J, Foucher N, Sevin C. A necropsy survey of neurologic diseases in 4,319 horses examined in normandy (France) from 1986 to 2006.. Journal of Equine Veterinary Science 2009;29(7):561–568.
      17. Levine JM, Scrivani PV, Divers TJ, Furr M, Mayhew IJ, Reed S, Levine GJ, Foreman JH, Boudreau C, Credille BC, Tennent-Brown B, Cohen ND. Multicenter case-control study of signalment, diagnostic features, and outcome associated with cervical vertebral malformation-malarticulation in horses.. J Am Vet Med Assoc 2010 Oct 1;237(7):812-22.
        pubmed: 20919847doi: 10.2460/javma.237.7.812google scholar: lookup
      18. Claridge HA, Piercy RJ, Parry A, Weller R. The 3D anatomy of the cervical articular process joints in the horse and their topographical relationship to the spinal cord.. Equine Vet J 2010 Nov;42(8):726-31.
        pubmed: 21039803doi: 10.1111/j.2042-3306.2010.00114.xgoogle scholar: lookup
      19. Ellingson BM, Ulmer JL, Kurpad SN, Schmit BD. Diffusion tensor MR imaging of the neurologically intact human spinal cord.. AJNR Am J Neuroradiol 2008 Aug;29(7):1279-84.
        pubmed: 18417607doi: 10.3174/ajnr.a1064google scholar: lookup
      20. Hudson NPH, Mayhew IG. Radiographic and myelographic assessment of the equine cervical vertebral column and spinal cord.. Equine Veterinary Education 2005;17(1):34–38.
      21. Taylor PM. Effects of surgery on endocrine and metabolic responses to anaesthesia in horses and ponies.. Res Vet Sci 1998 Mar-Apr;64(2):133-40.
        pubmed: 9625469doi: 10.1016/s0034-5288(98)90008-xgoogle scholar: lookup
      22. Dorph-Petersen KA, Lewis DA. Stereological approaches to identifying neuropathology in psychosis.. Biol Psychiatry 2011 Jan 15;69(2):113-26.
        pmc: PMC2974031pubmed: 20678756doi: 10.1016/j.biopsych.2010.04.030google scholar: lookup
      23. Nyengaard JR. Stereologic methods and their application in kidney research.. J Am Soc Nephrol 1999 May;10(5):1100-23.
        pubmed: 10232698doi: 10.1681/asn.v1051100google scholar: lookup
      24. Gundersen HJ, Jensen EB. The efficiency of systematic sampling in stereology and its prediction.. J Microsc 1987 Sep;147(Pt 3):229-63.
        pubmed: 3430576doi: 10.1111/j.1365-2818.1987.tb02837.xgoogle scholar: lookup
      25. Bolat D, Bahar S, Sur E, Selcuk ML, Tipirdamaz S. Selective gray and white matter staining of the horse spinal cord.. Kafkas Universitesi Veteriner Fakültesi Dergisi 2012;18(2):249–254.
      26. Sahin B, Ergur H. Assessment of the optimum section thickness for the estimation of liver volume using magnetic resonance images: a stereological gold standard study.. Eur J Radiol 2006 Jan;57(1):96-101.
        pubmed: 16112829doi: 10.1016/j.ejrad.2005.07.006google scholar: lookup
      27. Nickel R, Schummer A, Seiferle E. Lehrbuch der Anatomie der Haustiere: Nervensystem, Sinnesorgane, endokrine Drüsen.. 2004.
      28. Ocal MK, Haziroglu RM. Comparative morphological studies on the spinal cord of the donkey: I. The cross sectional areas of the spinal cord segments.. Ankara Üniversitesi Veteriner Fakültesi Dergisi 1988;35(1):55–68.
      29. Kahvecioglu K, Ozcan S, Cakır M. Anatomic studies on the medulla spinalis of the Angora goat (Excluding the coccygeal segments). Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 1995;6(1-2):76–80.
      30. Lima FC, Santos ALQ, Lima BC, Vieira LG, Hirano LQL. Topographic anatomy of the spinal cord and vertebromedullary relationships in Mazama gouazoubira Fisher, 1814 (Artiodactyla; Cervidae). Acta Scientiarum—Biological Sciences 2010;32(2):189–194.
      31. Barson AJ, Sands J. Regional and segmental characteristics of the human adult spinal cord.. J Anat 1977 Jul;123(Pt 3):797-803.
        pmc: PMC1234736pubmed: 885791
      32. Fletcher TF, Kitchell RL. Anatomical studies on the spinal cord segments of the dog.. Am J Vet Res 1966 Nov;27(121):1759-67.
        pubmed: 5971619
      33. Haziroglu RM, Ocal MK. Comparative morphological studies on the spinal cord of the donkey: II. The topography of the segments.. Ankara Üniversitesi Veteriner Fakültesi Dergisi 1988;35(2-3):476–487.
      34. Rao GS. Anatomical studies on the ovine spinal cord.. Anat Anz 1990;171(4):261-4.
        pubmed: 2080816
      35. Rao GS, Kalt DJ, Koch M, Majok AA. Anatomical studies on the spinal cord segments of the impala (Aepyceros melampus).. Anat Histol Embryol 1993 Sep;22(3):273-8.
        pubmed: 8238955doi: 10.1111/j.1439-0264.1993.tb00365.xgoogle scholar: lookup
      36. Kameyama T, Hashizume Y, Sobue G. Morphologic features of the normal human cadaveric spinal cord.. Spine (Phila Pa 1976) 1996 Jun 1;21(11):1285-90.
        pubmed: 8725917doi: 10.1097/00007632-199606010-00001google scholar: lookup
      37. Choi D, Carroll N, Abrahams P. Spinal cord diameters in cadaveric specimens and magnetic resonance scans, to assess embalming artefacts.. Surg Radiol Anat 1996;18(2):133-5.
        pubmed: 8782319doi: 10.1007/bf01795233google scholar: lookup
      38. Ishikawa M, Matsumoto M, Fujimura Y, Chiba K, Toyama Y. Changes of cervical spinal cord and cervical spinal canal with age in asymptomatic subjects.. Spinal Cord 2003 Mar;41(3):159-63.
        pubmed: 12612618doi: 10.1038/sj.sc.3101375google scholar: lookup
      39. Kameyama T, Hashizume Y, Ando T, Takahashi A. Morphometry of the normal cadaveric cervical spinal cord.. Spine (Phila Pa 1976) 1994 Sep 15;19(18):2077-81.
        pubmed: 7825049doi: 10.1097/00007632-199409150-00013google scholar: lookup
      40. Inoue H, Ohmori K, Takatsu T, Teramoto T, Ishida Y, Suzuki K. Morphological analysis of the cervical spinal canal, dural tube and spinal cord in normal individuals using CT myelography.. Neuroradiology 1996 Feb;38(2):148-51.
        pubmed: 8692426doi: 10.1007/bf00604802google scholar: lookup
      41. Yu YL, du Boulay GH, Stevens JM, Kendall BE. Morphology and measurements of the cervical spinal cord in computer-assisted myelography.. Neuroradiology 1985;27(5):399-402.
        pubmed: 4058732doi: 10.1007/bf00327602google scholar: lookup
      42. Bucciero A, Vizioli L, Tedeschi G. Cord diameters and their significance in prognostication and decisions about management of cervical spondylotic myelopathy.. J Neurosurg Sci 1993 Dec;37(4):223-8.
        pubmed: 7931646
      43. Milhorat TH, Kotzen RM, Anzil AP. Stenosis of central canal of spinal cord in man: incidence and pathological findings in 232 autopsy cases.. J Neurosurg 1994 Apr;80(4):716-22.
        pubmed: 8151352doi: 10.3171/jns.1994.80.4.0716google scholar: lookup
      44. Yasui K, Hashizume Y, Yoshida M, Kameyama T, Sobue G. Age-related morphologic changes of the central canal of the human spinal cord.. Acta Neuropathol 1999 Mar;97(3):253-9.
        pubmed: 10090672doi: 10.1007/s004010050982google scholar: lookup
      45. Marín-García P, González-Soriano J, Martinez-Sainz P, Contreras-Rodríguez J, Del Corral-Gros C, Rodríguez-Veiga E. Spinal cord central canal of the German shepherd dog: morphological, histological, and ultrastructural considerations.. J Morphol 1995 May;224(2):205-12.
        pubmed: 7745605doi: 10.1002/jmor.1052240209google scholar: lookup
      46. Trostle SS, Dubielzig RR, Beck KA. Examination of frozen cross sections of cervical spinal intersegments in nine horses with cervical vertebral malformation: lesions associated with spinal cord compression.. J Vet Diagn Invest 1993 Jul;5(3):423-31.
        pubmed: 8373859doi: 10.1177/104063879300500321google scholar: lookup
      47. Portiansky EL, Barbeito CG, Goya RG, Gimeno EJ, Zuccolilli GO. Morphometry of cervical segments grey matter in the male rat spinal cord.. J Neurosci Methods 2004 Oct 30;139(2):217-29.
        pubmed: 15488235doi: 10.1016/j.jneumeth.2004.04.031google scholar: lookup
      48. Kretschmann HJ, Tafesse U, Herrmann A. Different volume changes of cerebral cortex and white matter during histological preparation.. Microsc Acta 1982 May;86(1):13-24.
        pubmed: 7048029
      49. Quester R, Schröder R. The shrinkage of the human brain stem during formalin fixation and embedding in paraffin.. J Neurosci Methods 1997 Jul 18;75(1):81-9.
        pubmed: 9262148doi: 10.1016/s0165-0270(97)00050-2google scholar: lookup
      50. Mouritzen Dam A. Shrinkage of the brain during histological procedures with fixation in formaldehyde solutions of different concentrations.. J Hirnforsch 1979;20(2):115-9.
        pubmed: 556570

      Citations

      This article has been cited 2 times.
      1. Kondo T, Sato F, Tsuzuki N, Yamada K. Sex differences in cervical spinal cord and spinal canal development in Thoroughbred horses. J Vet Med Sci 2022 Sep 21;84(10):1363-1367.
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      2. Selcuk ML, Kayikci F. Anatomical and Embryological Development of the Chick Cerebrum in Different Embryonic Periods. Vet Med Sci 2025 Jan;11(1):e70124.
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