Three-Dimensional Coculture of Meniscal Cells and Mesenchymal Stem Cells in Collagen Type I Hydrogel on a Small Intestinal Matrix-A Pilot Study Toward Equine Meniscus Tissue Engineering.
Abstract: Meniscal injuries are the most frequently encountered soft tissue injuries in the equine stifle joint. Due to the inherent limited repair potential of meniscal tissue, meniscal injuries do not only affect the meniscus itself but also lead to impaired joint homeostasis and secondary osteoarthritis. The presented study compares 3D coculture constructs of primary equine mesenchymal stem cells (MSC) and meniscus cells (MC) seeded on three different scaffolds-a cell-laden collagen type I hydrogel (Col I gel), a tissue-derived small intestinal matrix scaffold (SIS-muc) and a combination thereof-for their qualification to be applied for meniscus tissue engineering. To investigate cell attachment of primary MC and MSC on SIS-muc matrix SEM pictures were performed. For molecular analysis, lyophilized samples of coculture constructs with different cell ratios (100% MC, 100% MSC, and 50% MC and 50% MSC, 20% MC, and 80% MSC) were digested and analyzed for DNA and GAG content. Active matrix remodeling of 3D coculture models was indicated by matrix metalloproteinases detection. For comparison of tissue-engineered constructs with the histologic architecture of natural equine menisci, paired lateral and medial menisci of 15 horses representing different age groups were examined. A meniscus phenotype with promising similarity to native meniscus tissue in its GAG/DNA expression in addition to Col I, Col II, and Aggrecan production was achieved using a scaffold composed of Col I gel on SIS-muc combined with a coculture of MC and MSC. The results encourage further development of this scaffold-cell combination for meniscus tissue engineering.
Publication Date: 2017-04-07 PubMed ID: 28095754DOI: 10.1089/ten.TEA.2016.0317Google Scholar: Lookup
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- Journal Article
Summary
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This research focuses on tissue engineering solutions to repair common soft tissue injuries in horses, specifically damage to the meniscus. By comparing different three-dimensional (3D) coculture constructs, the researchers aimed to find the most effective combination of scaffolding and cells for use in restoring damaged equine meniscus tissue.
About the Problem
- Meniscal injuries are typical in equine stifle joints, a crucial part of a horse’s anatomy. The inherent limited repair potential of meniscal tissue means that not only does the specific injury impact the meniscus, but it also disrupts joint homeostasis, leading to secondary osteoarthritis.
Research Methodology
- The researchers conducted a comparative study of 3D coculture constructs. These consist of primary equine mesenchymal stem cells (MSC) and meniscus cells (MC), seeded on three different scaffolds: a collagen type I hydrogel (Col I gel), a tissue-derived small intestinal matrix scaffold (SIS-muc), and a combination of the two.
- The team investigated cell attachment of MC and MSC on the SIS-muc matrix using scanning electron microscopy (SEM) images.
- Molecular analysis was performed on freeze-dried samples of coculture constructs with varying cell ratios. Researchers checked for DNA and proteoglycan (GAG) content, essential components of the extracellular matrix in cartilage.
- Evidence of matrix remodeling in 3D coculture models was sought via the detection of matrix metalloproteinases, a family of enzymes that degrade the matrix.
- The team compared the engineered tissue constructs with the histological architecture of natural equine menisci by examining paired lateral and medial menisci from 15 horses of different ages.
Results and Conclusion
- The researchers found that a specific phenotype of the meniscus that closely resembled the native meniscus tissue in terms of its GAG/DNA expression and production of Col I, Col II, and Aggrecan, was created using a scaffold composed of Col I gel on SIS-muc with a coculture of MC and MSC.
- These findings support further development of this specific scaffold-cell combination for potential use in meniscus tissue engineering to treat meniscal injuries in horses.
Cite This Article
APA
Kremer A, Ribitsch I, Reboredo J, Dürr J, Egerbacher M, Jenner F, Walles H.
(2017).
Three-Dimensional Coculture of Meniscal Cells and Mesenchymal Stem Cells in Collagen Type I Hydrogel on a Small Intestinal Matrix-A Pilot Study Toward Equine Meniscus Tissue Engineering.
Tissue Eng Part A, 23(9-10), 390-402.
https://doi.org/10.1089/ten.TEA.2016.0317 Publication
Researcher Affiliations
- 1 Department of Tissue Engineering and Regenerative Medicine (TERM), University Hospital Wuerzburg , Wuerzburg, Germany .
- 2 Translational Center Wuerzburg 'Regenerative therapies,' Wuerzburg Branch of the Fraunhofer IGB, Wuerzburg, Germany .
- 3 Vienna Equine Tissue Engineering and Regenerative Medicine, Equine Clinic, University of Veterinary Medicine Vienna , Vienna, Austria .
- 4 Department of Companion Animals and Horses, University of Veterinary Medicine Vienna , Vienna, Austria .
- 1 Department of Tissue Engineering and Regenerative Medicine (TERM), University Hospital Wuerzburg , Wuerzburg, Germany .
- 2 Translational Center Wuerzburg 'Regenerative therapies,' Wuerzburg Branch of the Fraunhofer IGB, Wuerzburg, Germany .
- 5 Department of Pathobiology, Institute of Histology & Embryology, University of Veterinary Medicine Vienna , Vienna, Austria .
- 5 Department of Pathobiology, Institute of Histology & Embryology, University of Veterinary Medicine Vienna , Vienna, Austria .
- 3 Vienna Equine Tissue Engineering and Regenerative Medicine, Equine Clinic, University of Veterinary Medicine Vienna , Vienna, Austria .
- 4 Department of Companion Animals and Horses, University of Veterinary Medicine Vienna , Vienna, Austria .
- 1 Department of Tissue Engineering and Regenerative Medicine (TERM), University Hospital Wuerzburg , Wuerzburg, Germany .
- 2 Translational Center Wuerzburg 'Regenerative therapies,' Wuerzburg Branch of the Fraunhofer IGB, Wuerzburg, Germany .
MeSH Terms
- Animals
- Cells, Cultured
- Coculture Techniques
- Collagen Type I / chemistry
- Extracellular Matrix / chemistry
- Horses
- Hydrogels / chemistry
- Intestine, Small / chemistry
- Meniscus / injuries
- Meniscus / metabolism
- Mesenchymal Stem Cells / metabolism
- Pilot Projects
- Tissue Engineering
Citations
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