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Home / Equine Research Bank / Animals (Basel) / Three Manual Noncommercial Methods to Prepare Equine Platele...
Animals : an open access journal from MDPI2021; 11(6); doi: 10.3390/ani11061478

Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma.

Abstract: In light of PRP's increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (n = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (p < 0.05), and the latter also had greater contamination with WBC than the others (p < 0.001). Platelet viability was similar across treatments (p > 0.05). Cooling for 24 h did not affect platelet counts in all methods (p > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (p = 0.04), and agglutination increased over time in all methods (p < 0.001). The three methods increased (1.8-5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP's clinical efficacy.
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Publication Date: 2021-05-21 PubMed ID: 34063777PubMed Central: PMC8223772DOI: 10.3390/ani11061478Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates three manual noncommercial ways to prepare equine Platelet-rich Plasma (PRP) and evaluates their effectiveness by looking at factors like platelet concentration, cell contamination, and platelet viability after cooling.

Research Methodologies

  • The study involved blood samples from 18 clinically healthy mares. Three preparation methods were used. Method 1 used a blood transfusion bag with double centrifugation. Method 2 involved blood tubes with one centrifugation. Method 3 used a syringe, allowing it to sediment for 4 hours while held upright.
  • The PRP and platelet-poor plasma (PPP) derived from these methods were then evaluated for red and white blood cell counts, platelet counts, and their viability.
  • For a subset of six mares, samples prepared through all three methods were cooled at 5°C and evaluated after 6 and 24 hours.

Research Findings

  • The method using the blood transfusion bag (Method 1) resulted in the highest platelet concentration, while the method using the syringe (Method 3) resulted in the lowest.
  • Method 3 also had higher contamination with white blood cells (WBC) compared to the other two methods.
  • The viability of the platelets was found to be similar across all three methods.
  • Cooling the PRP for 24 hours did not affect the platelet counts in any of the methods. However, platelet viability was reduced after cooling the PRP produced by the syringe method (Method 3).
  • All three methods were capable of raising the platelet concentration in the PRP when compared to whole blood, without compromising the viability of the platelets.

Conclusion

  • The research concludes that all three manual methods effectively concentrated platelets, but cooling affected their viability. However, it remains unclear if the differences in methods and the cooling process would impact the clinical effectiveness of the PRP.

Cite This Article

APA
Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. (2021). Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals (Basel), 11(6). https://doi.org/10.3390/ani11061478

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 6

Researcher Affiliations

Segabinazzi, Lorenzo G T M
  • Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA.
  • School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618681, SP, Brazil.
Podico, Giorgia
  • Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA.
  • Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA.
Rosser, Michael F
  • Department of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA.
Nanjappa, Som G
  • Department of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA.
Alvarenga, Marco A
  • School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618681, SP, Brazil.
Canisso, Igor F
  • Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA.
  • Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 5 times.
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