Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae.
Abstract: Glanders, caused by (.) is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against are required. To date, the complement fixation test (CFT) is the mandatory test to issue these health certificates. The CFT is difficult to standardize and, due to its poor specificity, often leads to false-positive reactions resulting in trade restrictions with considerable financial consequences. In the present study, the new ID Screen Glanders Double Antigen Multispecies ELISA (GLANDA- ELISA) (IDvet, Grabels, France) was evaluated using 400 negative and 370 glanders positive field samples of equidae. The GLANDA-ELISA was significantly more specific (99.8%) than the CFT (97.0%). Considering the comparable sensitivities of CFT (96.5%) and ELISA (98.1%), this new GLANDA-ELISA test appears a suitable confirmatory test and a realistic alternative for serological testing of horses for trade or movement.
Copyright © 2021 Elschner, Melzer, Singha, Muhammad, Gardner and Neubauer.
Publication Date: 2021-02-16 PubMed ID: 33665218PubMed Central: PMC7920956DOI: 10.3389/fvets.2021.628389Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Antibodies
- Burkholderia mallei
- Complement Fixation
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equids
- Equine Diseases
- Equine Health
- Glanders
- Infectious Disease
- Public Health
- Serology
- Veterinary Medicine
- Veterinary Procedure
- Veterinary Research
- Veterinary Science
- Zoonotic Diseases
Summary
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The research study focuses on validating a commercial glanders ELISA test as an effective alternative to the traditional CFT in controlling the international trade of equids. The new ELISA test exhibits higher specificity than the CFT, potentially reducing false-positives and associated financial consequences.
Objective of the Research
- The primary aim of this research is to evaluate and validate the use of a new commercial glanders ELISA (GLANDA-ELISA) developed by IDvet, over the traditional CFT for the international trade and movement of equids.
- The researchers endeavor to test the ELISA’s accuracy and effectiveness, focusing on its specificity and sensitivity compared to the CFT.
About Glanders and its Diagnostic Issues
- Glanders is a contagious zoonotic disease affecting equidae caused by the bacterium Burkholderia mallei. It is notifiable, meaning any instance of this disease has to be reported to veterinary authorities.
- For the international trade and movement of equids, negative results for antibodies against B. mallei are required. Currently, these results are obtained through the complement fixation test (CFT).
- However, the CFT test has been found to be difficult to standardize and often leads to poor specificity, which leads to false-positive reactions. The false positives result in trade restrictions leading to financial losses.
Evaluation of the GLANDA-ELISA Test
- The validation process of the GLANDA-ELISA involved testing 400 negative and 370 glanders positive field samples of equidae.
- Results showed that the new ELISA test was significantly more specific (99.8%) than the CFT (97.0%). This high specificity can reduce the number of false positive reactions, thereby decreasing unnecessary trade restrictions and associated financial implications.
Sensitivity and Suitability of GLANDA-ELISA
- The research also reported comparable sensitivities for the CFT (96.5%) and the ELISA (98.1%), i.e., they both effectively identified true positive samples.
- This equivalency in sensitivity, combined with ELISA’s significantly better specificity, makes the GLANDA-ELISA a viable alternative for serological testing of horses for trade or movement.
Cite This Article
APA
Elschner MC, Melzer F, Singha H, Muhammad S, Gardner I, Neubauer H.
(2021).
Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae.
Front Vet Sci, 8, 628389.
https://doi.org/10.3389/fvets.2021.628389 Publication
Researcher Affiliations
- Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Jena, Germany.
- Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Jena, Germany.
- Indian Council of Agricultural Research, National Research Centre on Equines, Hisar, India.
- Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan.
- Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island (UPEI), Charlottetown, PE, Canada.
- Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Jena, Germany.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
This article includes 27 references
- Ghori MT, Khan MS, Khan JA, Rabbani M, Shabbir MZ, Chaudhry HR, Ali MA, Muhammad J, Elschner MC, Jayarao BM. Seroprevalence and risk factors of glanders in working equines - Findings of a cross-sectional study in Punjab province of Pakistan.. Acta Trop 2017 Dec;176:134-139.
- Arun S, Neubauer H, Gu00fcrel A, Ayyildiz G, Kusu00e7u B, Yesildere T, Meyer H, Hermanns W. Equine glanders in Turkey.. Vet Rec 1999 Mar 6;144(10):255-8.
- Mota RA, da Fonseca Oliveira AA, da Silva AM, Junior JW, da Silva LB, de Farias Brito M, Rabelo SS. Glanders in donkeys (Equus Asinus) in the state of pernambuco, Brazil: A case report.. Braz J Microbiol 2010 Jan;41(1):146-9.
- Malik P, Singha H, Goyal SK, Khurana SK, Tripathi BN, Dutt A, Singh D, Sharma N, Jain S. Incidence of Burkholderia mallei infection among indigenous equines in India.. Vet Rec Open 2015;2(2):e000129.
- Singha H, Shanmugasundaram K, Tripathi BN, Saini S, Khurana SK, Kanani A, Shah N, Mital A, Kanwar P, Bhatt L, Limaye V, Khasa V, Arora R, Gupta S, Sangha S, Sharma H, Agarwal SK, Tapase J, Parnam S, Dubey P, Baalasundaram SK, Mandal BN, Virmani N, Gulati BR, Malik P. Serological surveillance and clinical investigation of glanders among indigenous equines in India from 2015 to 2018.. Transbound Emerg Dis 2020 May;67(3):1336-1348.
- Erdemsurakh O, Ochirbat K, Gombosuren U, Tserendorj B, Purevdorj B, Vanaabaatar B, Aoshima K, Kobayashi A, Kimura T. Seroprevalence of equine glanders in horses in the central and eastern parts of Mongolia.. J Vet Med Sci 2020 Sep 24;82(9):1247-1252.
- Scholz HC, Pearson T, Hornstra H, Projahn M, Terzioglu R, Wernery R, Georgi E, Riehm JM, Wagner DM, Keim PS, Joseph M, Johnson B, Kinne J, Jose S, Hepp CM, Witte A, Wernery U. Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events.. PLoS Negl Trop Dis 2014 Sep;8(9):e3195.
- OIE . Chapter 2.5.11: Glanders and melioidosis. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. (2018). Available online at: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.05.11_GLANDERS.pdf (accessed January 25, 2021).
- de Carvalho Filho MB, Ramos RM, Fonseca AA Jr, de Lima Orzil L, Sales ML, de Assis Santana VL, de Souza MM, Dos Reis Machado E, Filho PR, Leite RC, Dos Reis JK. Development and validation of a method for purification of mallein for the diagnosis of glanders in equines.. BMC Vet Res 2012 Sep 2;8:154.
- da Silva KP, de Campos Takaki GM, da Silva LB, Saukas TN, Santos AS, Mota RA. Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules.. Braz J Microbiol 2013;44(1):179-81.
- Adhikari N, Acharya KP, Wilson RT. The potential for an outbreak of glanders in Nepal.. Trop Med Health 2019;47:57.
- Neubauer H, Sprague LD, Zacharia R, Tomaso H, Al Dahouk S, Wernery R, Wernery U, Scholz HC. Serodiagnosis of Burkholderia mallei infections in horses: state-of-the-art and perspectives.. J Vet Med B Infect Dis Vet Public Health 2005 Jun;52(5):201-5.
- Elschner MC, Scholz HC, Melzer F, Saqib M, Marten P, Rassbach A, Dietzsch M, Schmoock G, de Assis Santana VL, de Souza MM, Wernery R, Wernery U, Neubauer H. Use of a Western blot technique for the serodiagnosis of glanders.. BMC Vet Res 2011 Jan 19;7:4.
- Elschner MC, Laroucau K, Singha H, Tripathi BN, Saqib M, Gardner I, Saini S, Kumar S, El-Adawy H, Melzer F, Khan I, Malik P, Sauter-Louis C, Neubauer H. Evaluation of the comparative accuracy of the complement fixation test, Western blot and five enzyme-linked immunosorbent assays for serodiagnosis of glanders.. PLoS One 2019;14(4):e0214963.
- Khan I, Wieler LH, Melzer F, Gwida M, Santana VL, de Souza MM, Saqib M, Elschner MC, Neubauer H. Comparative evaluation of three commercially available complement fixation test antigens for the diagnosis of glanders.. Vet Rec 2011 Nov 5;169(19):495.
- Khan I, Wieler LH, Saqib M, Melzer F, Santana VL, Neubauer H, Elschner MC. Effect of incubation temperature on the diagnostic sensitivity of the glanders complement fixation test.. Rev Sci Tech 2014 Dec;33(3):869-75.
- Laroucau K, Colaneri C, Jau00ff M, Corde Y, Drapeau A, Durand B, Zientara S, Beck C. Interlaboratory ring trial to evaluate CFT proficiency of European laboratories for diagnosis of glanders in equids.. Vet Rec 2016 Jun 18;178(25):632.
- Abreu DC, Gomes AS, Tessler DK, Chiebao DP, Fava CD, Romaldini AHCN, Araujo MC, Pompei J, Marques GF, Harakava R, Pituco EM, Nassar AFC. Systematic monitoring of glanders-infected horses by complement fixation test, bacterial isolation, and PCR.. Vet Anim Sci 2020 Dec;10:100147.
- Laroucau K, Saqib M, Martin B, Deshayes T, Bertin C, Wernery U, Joseph S, Singha H, Tripathi BN, Beck C. Development of a microsphere-based immunoassay for the serological detection of glanders in equids.. Acta Trop 2020 Jul;207:105463.
- Singha H, Malik P, Goyal SK, Khurana SK, Mukhopadhyay C, Eshwara VK, Singh RK. Optimization and validation of indirect ELISA using truncated TssB protein for the serodiagnosis of glanders amongst equines.. ScientificWorldJournal 2014;2014:469407.
- Khan I, Elschner MC, Melzer F, Gwida M, Wieler LH, Ali R, Saqib M, Neubauer H. Performance of complement fixation test and confirmatory immunoblot as two-cascade testing approach for serodiagnosis of glanders in an endemic region of South East Asia.. Berl Munch Tierarztl Wochenschr 2012 Mar-Apr;125(3-4):117-21.
- Dohoo I MW, Stryhn H. Veterinary Epidemiologic Research. Charlottetown, PE: VER Inc. (2009).
- Povitzky OR. Prompt macroscopic agglutination in the diagnosis of glanders. J Immunol. (1918) 3:463u201379.
- Yazdansetad S, Mosavari N, Tadayon K, Mehregan I. Development of an immunoblotting assay for serodiagnosis of Burkholderia mallei infection: the whole-cell proteome-based paradigm.. Iran J Microbiol 2019 Jun;11(3):232-238.
- Singh S, Dohre SK, Kamthan A, Pal V, Karothia BS, Singha HS, Kumar S. Improvement of recombinant-truncated Burkholderia motility protein A (BimA)-based indirect ELISA for equine glanders.. J Immunoassay Immunochem 2018;39(5):565-575.
- Pal V, Kumar S, Malik P, Rai GP. Evaluation of recombinant proteins of Burkholderia mallei for serodiagnosis of glanders.. Clin Vaccine Immunol 2012 Aug;19(8):1193-8.
- Jacobson RH. Validation of serological assays for diagnosis of infectious diseases.. Rev Sci Tech 1998 Aug;17(2):469-526.
Citations
This article has been cited 3 times.- Wagner GE, Berner A, Lipp M, Kohler C, Assig K, Lichtenegger S, Saqib M, Mu00fcller E, Trinh TT, Gad AM, Su00f6ffing HH, Ehricht R, Laroucau K, Steinmetz I. Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics.. J Clin Microbiol 2023 Jan 26;61(1):e0123422.
- P K, M M, S M, Kr P, T D, G W, Ma V, K L. First glanders cases detected in Nepal underscore the need for surveillance and border controls.. BMC Vet Res 2022 Apr 6;18(1):132.
- Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Schmidt CG, Herskin M, Michel V, Miranda Chueca Mu00c1, Padalino B, Pasquali P, Spoolder H, Stu00e5hl K, Velarde A, Viltrop A, Winckler C, Gubbins S, Laroucau K, Antoniou SE, Aznar I, Broglia A, Lima E, Van der Stede Y, Zancanaro G, Roberts HC. Assessment of the control measures of the category A diseases of Animal Health Law: Burkholderia mallei (Glanders).. EFSA J 2022 Jan;20(1):e07069.