Zinc Status of Horses and Ponies: Relevance of Health, Horse Type, Sex, Age, and Test Material.
Abstract: Little is known about the animal- and diet-related factors that could interfere with the plasma zinc (Zn) concentrations of equines. Additionally, the adequacy of plasma to reflect changes in the Zn intake is unclear. In the first part of this study, the plasma Zn concentrations of hospitalized horses and ponies ( = 538) were measured and evaluated for the impact of the age, sex, horse type, and internal diseases of the animals. In the second part, the effects of increasing dietary Zn chloride hydroxide and Zn methionine supplementations were assessed on the plasma and mane hair Zn concentrations of healthy horses ( = 2) and ponies ( = 8). Part 1: The age, sex, and horse type did not influence the plasma Zn concentrations. No effect of internal diseases was observed, with the exception of higher plasma Zn concentrations in animals with metabolic disorders compared to the control group ( < 0.05). Part 2: Both Zn supplements dose-dependently increased the Zn concentrations in the mane hair ( = 0.003), but not in the plasma of the horses and ponies. In conclusion, the plasma Zn concentrations were widely unaffected by nutritional and non-nutritional factors in equines, while mane hair samples better reflected the dietary Zn supply.
Publication Date: 2023-04-16 PubMed ID: 37104450PubMed Central: PMC10144230DOI: 10.3390/vetsci10040295Google Scholar: Lookup
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Summary
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The study examines the factors that influence zinc (Zn) levels in horses and ponies, and finds that hair samples rather than plasma samples effectively reflect dietary zinc intake.
Study Purpose and Methodology
- The study aims to explore the factors, both diet and non-diet related, that might influence the concentration of zinc in the plasma of equines (horses and ponies). It also attempts to ascertain whether plasma reflects changes in zinc intake.
- In the first part of the study, the researchers examine plasma zinc concentrations in a large sample size of 538 hospitalized horses and ponies. They then assess the influences of variables such as age, sex, type of horse, and internal diseases on these concentrations.
- In the second part of the study, the researchers investigate the effect of increasing dietary supplements of zinc chloride hydroxide and zinc methionine on the zinc concentrations in the plasma and mane hair of 2 healthy horses and 8 healthy ponies.
Study Findings
- The first part of the study finds no significant correlation between age, sex, type of horse and plasma zinc concentrations. The plasma zinc concentration levels remain unaffected even in the presence of internal diseases, with the exception of metabolic disorders that caused an increase in the plasma zinc concentrations when compared to the control group.
- The results of the second part of the study show that both zinc supplements tested had a dose-dependent positive effect on the zinc concentrations in the mane hair, albeit with no significant effect on the plasma concentrations of the horses and ponies.
Conclusion
- The study concludes that plasma zinc concentrations in horses and ponies remain widely unaffected by both nutritional and non-nutritional factors.
- The research suggests that mane hair samples provide a more accurate measure of dietary zinc supply in equines, as they reflect the changes in zinc intake far better than plasma samples do.
Cite This Article
APA
van Bömmel-Wegmann S, Gehlen H, Barton AK, Büttner K, Zentek J, Paßlack N.
(2023).
Zinc Status of Horses and Ponies: Relevance of Health, Horse Type, Sex, Age, and Test Material.
Vet Sci, 10(4), 295.
https://doi.org/10.3390/vetsci10040295 Publication
Researcher Affiliations
- Institute of Animal Nutrition, Freie Universität Berlin, 14195 Berlin, Germany.
- Equine Clinic, Freie Universität Berlin, 14163 Berlin, Germany.
- Equine Clinic, Freie Universität Berlin, 14163 Berlin, Germany.
- Unit for Biomathematics and Data Processing, Justus-Liebig-University Giessen, 35392 Giessen, Germany.
- Institute of Animal Nutrition, Freie Universität Berlin, 14195 Berlin, Germany.
- Institute of Animal Nutrition, Freie Universität Berlin, 14195 Berlin, Germany.
Grant Funding
- This research was funded by almapharm GmbH & Co. KG (Wildpoldsried, Germany). / This research was funded by almapharm GmbH & Co. KG (Wildpoldsried, Germany).
Conflict of Interest Statement
Sarah van Bömmel-Wegmann received a scholarship funded by almapharm GmbH & Co. KG (Wildpoldsried, Germany). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
References
This article includes 50 references
- Frassinetti S, Bronzetti G, Caltavuturo L, Cini M, Croce CD. The role of zinc in life: a review.. J Environ Pathol Toxicol Oncol 2006;25(3):597-610.
- Baltaci AK, Mogulkoc R, Baltaci SB. Review: The role of zinc in the endocrine system.. Pak J Pharm Sci 2019 Jan;32(1):231-239.
- National Research Council (NRC) Nutrient Requirements of Horses. 6th ed. The National Academic Press; Washington, DC, USA: 2007.
- King JC. Assessment of zinc status.. J Nutr 1990 Nov;120 Suppl 11:1474-9.
- Hartoma R. Serum testosterone compared with serum zinc in man.. Acta Physiol Scand 1977 Nov;101(3):336-41.
- Buxaderas SC, Farré-Rovira R. Whole blood and serum zinc levels in relation to sex and age.. Rev Esp Fisiol 1985 Dec;41(4):463-70.
- Schuhmacher M, Domingo JL, Corbella J. Zinc and copper levels in serum and urine: relationship to biological, habitual and environmental factors.. Sci Total Environ 1994 May 30;148(1):67-72.
- Arnaud J, Touvier M, Galan P, Andriollo-Sanchez M, Ruffieux D, Roussel AM, Hercberg S, Favier A. Determinants of serum zinc concentrations in a population of French middle-age subjects (SU.VI.MAX cohort).. Eur J Clin Nutr 2010 Oct;64(10):1057-64.
- Cymbaluk NF, Bristol FM, Christensen DA. Influence of age and breed of equid on plasma copper and zinc concentrations.. Am J Vet Res 1986 Jan;47(1):192-5.
- Stark G, Schneider B, Gemeiner M. Zinc and copper plasma levels in Icelandic horses with Culicoides hypersensitivity.. Equine Vet J 2001 Sep;33(5):506-9.
- Maia L, de Souza M.V, Bragança Alves Fernandes R, Ferreira Fontes M.P, de Souza Vianna M.W, Vercelli Luz W. Heavy Metals in Horse Blood, Serum, and Feed in Minas Gerais, Brazil. J. Equine Vet. Sci. 2006;26:578–583.
- Kolm G, Helsberg A, Gemeiner M. Variations in the concentration of zinc in the blood of Icelandic horses.. Vet Rec 2005 Oct 29;157(18):549-51.
- Murase H, Sakai S, Kusano K, Hobo S, Nambo Y. Serum zinc levels and their relationship with diseases in racehorses.. J Vet Med Sci 2013 Jan 31;75(1):37-41.
- Cymbaluk NF, Christensen DA. Copper, zinc and manganese concentrations in equine liver, kidney and plasma.. Can Vet J 1986 May;27(5):206-10.
- Brummer-Holder M, Cassill BD, Hayes SH. Interrelationships Between Age and Trace Element Concentration in Horse Mane Hair and Whole Blood.. J Equine Vet Sci 2020 Apr;87:102922.
- Dunnett M, Lees P. Trace element, toxin and drug elimination in hair with particular reference to the horse.. Res Vet Sci 2003 Oct;75(2):89-101.
- Combs DK. Hair analysis as an indicator of mineral status of livestock.. J Anim Sci 1987 Dec;65(6):1753-8.
- Wells L.A, LeRoy R, Ralston S.L. Mineral intake and hair analysis of horses in Arizona. J. Equine Vet. Sci. 1990;10:412–416.
- Ghorbani A, Mohit A, Kuhi H.D. Effects of dietary mineral intake on hair and serum mineral contents of horses. J. Equine Vet. Sci. 2015;35:295–300.
- Kalashnikov VV, Zajcev AM, Atroshchenko MM, Miroshnikov SA, Zavyalov OA, Frolov AN, Skalny AV. Assessment of Gender Effects and Reference Values of Mane Hair Trace Element Content in English Thoroughbred Horses (North Caucasus, Russia) Using ICP-DRC-MS.. Biol Trace Elem Res 2019 Oct;191(2):382-388.
- Asano K, Suzuki K, Chiba M, Sera K, Matsumoto T, Asano R, Sakai T. Influence of the coat color on the trace elemental status measured by particle-induced X-ray emission in horse hair.. Biol Trace Elem Res 2005 Feb;103(2):169-76.
- Asano R, Suzuki K, Otsuka T, Otsuka M, Sakurai H. Concentrations of toxic metals and essential minerals in the mane hair of healthy racing horses and their relation to age.. J Vet Med Sci 2002 Jul;64(7):607-10.
- Asano K, Suzuki K, Chiba M, Sera K, Asano R, Sakai T. Twenty-eight element concentrations in mane hair samples of adult riding horses determined by particle-induced X-ray emission.. Biol Trace Elem Res 2005 Nov;107(2):135-40.
- Armelin M.J.A, Ávila R.L, Piasentin R.M, Saiki M. Effect of chelated mineral supplementation on the absorption of Cu, Fe, K, Mn and Zn in horse hair. J. Radioanal. Nucl. Chem. 2003;258:449–451.
- van der Merwe D, van den Wollenberg L, van Hees-Valkenborg J, de Haan T, van der Drift S, Vandendriessche V. Evaluation of hair analysis for determination of trace mineral status and exposure to toxic heavy metals in horses in the Netherlands.. J Vet Diagn Invest 2022 Nov;34(6):1000-1005.
- Ozougwu J.C. Physiology of the liver. Int. J. Res. Pharm. Biosci. 2017;4:13–24.
- Wahl L, Vervuert I. Commercial Hair Analysis in Horses: A Tool to Assess Mineral Intake?. J Equine Vet Sci 2022 Dec;119:104145.
- Paßlack N, van Bömmel-Wegmann S, Vahjen W, Zentek J. Impact of Dietary Zinc Chloride Hydroxide and Zinc Methionine on the Faecal Microbiota of Healthy Adult Horses and Ponies.. J Equine Vet Sci 2022 Mar;110:103804.
- Gesellschaft für Ernährungsphysiologie (GfE) [Society for Nutritional Physiology] Flachowsky G., Kamphues J., Rodehutscord M., Schenkel H., Staudacher W., Südekum K.H., Susenbeth A., Windisch W. Empfehlungen zur Energie- und Nährstoffversorgung von Pferden [Recommendations for the Energy and Nutrient Supply of Horses] 1st ed. DLG-Verlag; Frankfurt am Main, Germany: 2014.
- Kaneko J.J., Harvey J.W., Bruss M.L. Clinical Biochemistry of Domestic Animals. 6th ed. Academic Press; San Diego, CA, USA: 2008.
- Jablan J, Grdić Rajković M, Inić S, Petlevski R, Domijan A.-M. Impact of Anticoagulants on Assessment of Zinc in Plasma. Croat. Chem. Acta. 2018;91:317–321.
- Hussein HA, Müller AE, Staufenbiel R. Comparative evaluation of mineral profiles in different blood specimens of dairy cows at different production phases.. Front Vet Sci 2022;9:905249.
- Katayev A, Balciza C, Seccombe DW. Establishing reference intervals for clinical laboratory test results: is there a better way?. Am J Clin Pathol 2010 Feb;133(2):180-6.
- Friedrichs KR, Harr KE, Freeman KP, Szladovits B, Walton RM, Barnhart KF, Blanco-Chavez J. ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics.. Vet Clin Pathol 2012 Dec;41(4):441-53.
- Helgeland K, Haider T, Jonsen J. Copper and zinc in human serum in Norway. Relationship to geography, sex and age.. Scand J Clin Lab Invest 1982 Feb;42(1):35-9.
- Gil-Extremera B, Maldonado A, Ruiz-Martínez M, Rubio MA. Zinc in adult human serum in Spain.. Rev Esp Fisiol 1989 Sep;45(3):217-9.
- Paz de Moncada N, Chacín de Bonilla L, Villasmil JJ, Estévez J, Bonilla E. [Blood levels of zinc in normal subjects].. Invest Clin 1989;30(2):101-9.
- Díaz Romero C, Henríquez Sánchez P, López Blanco F, Rodríguez Rodríguez E, Serra Majem L. Serum copper and zinc concentrations in a representative sample of the Canarian population.. J Trace Elem Med Biol 2002;16(2):75-81.
- Farzin L, Moassesi ME, Sajadi F, Amiri M, Shams H. Serum levels of antioxidants (Zn, Cu, Se) in healthy volunteers living in Tehran.. Biol Trace Elem Res 2009 Summer;129(1-3):36-45.
- Dabbaghmanesh MH, Taheri Boshrooyeh H, Kalantarhormozi MR, Ranjbar Omrani GH. Assessment of zinc concentration in random samples of the adult population in shiraz, iran.. Iran Red Crescent Med J 2011 Apr;13(4):249-55.
- Hotz C, Peerson JM, Brown KH. Suggested lower cutoffs of serum zinc concentrations for assessing zinc status: reanalysis of the second National Health and Nutrition Examination Survey data (1976-1980).. Am J Clin Nutr 2003 Oct;78(4):756-64.
- Pinna K, Woodhouse LR, Sutherland B, Shames DM, King JC. Exchangeable zinc pool masses and turnover are maintained in healthy men with low zinc intakes.. J Nutr 2001 Sep;131(9):2288-94.
- Auer DE, Ng JC, Thompson HL, Inglis S, Seawright AA. Acute phase response in horses: changes in plasma cation concentrations after localised tissue injury.. Vet Rec 1989 Mar 11;124(10):235-9.
- Kienzle E, Bockhorni T. [Nutrition of horses with equine pituitary pars intermedia dysfunction ("Cushing's syndrome") treated with pergolid - A field study].. Tierarztl Prax Ausg G Grosstiere Nutztiere 2018 Aug;46(4):249-256.
- Gromadzka-Ostrowska J, Zalewska B, Jakubów K, Gozlinski H. Three-year study on trace mineral concentration in the blood plasma of Shetland pony mares.. Comp Biochem Physiol A Comp Physiol 1985;82(3):651-60.
- Ali F, Lodhi L.A, Qureshi Z.I, Ahmad I, Hussain R. Serum mineral profile in various reproductive phases of mares. Pak. Vet. J. 2013;33:296–299.
- Whittem T, Davis C, Beresford GD, Gourdie T. Detection of morphine in mane hair of horses.. Aust Vet J 1998 Jun;76(6):426-7.
- Nitrayova S, Windisch W, von Heimendahl E, Müller A, Bartelt J. Bioavailability of zinc from different sources in pigs.. J Anim Sci 2012 Dec;90 Suppl 4:185-7.
- Huang Y.L, Lu L, Xi J.J, Li S.F, Li X.L, Liu S.B, Zhang L.Y, Xi L, Luo X.G. Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed diets with low or high phytate content. Anim. Feed Sci. Technol. 2013;179:144–148.
- van Bömmel-Wegmann S, Zentek J, Gehlen H, Barton AK, Paßlack N. Effects of dietary zinc chloride hydroxide and zinc methionine on the immune system and blood profile of healthy adult horses and ponies.. Arch Anim Nutr 2023 Feb;77(1):17-41.
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