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Experimental cell research.
Publisher:
Academic Press.. Orlando Fl : Academic Press
Frequency: Nineteen a year, 2001-
Country: United States
Language: English
Author(s):
International Society for Cell Biology.
Start Year:1950 -
ISSN:
0014-4827 (Print)
1090-2422 (Electronic)
0014-4827 (Linking)
1090-2422 (Electronic)
0014-4827 (Linking)
Impact Factor
3.7
2022
| NLM ID: | 0373226 |
| (DNLM): | E19320000(s) |
| (OCoLC): | 01568634 |
| Coden: | ECREA |
| Classification: | W1 EX501 |
Serum-free medium modulates the immunomodulatory and anabolic function of equine bone marrow-derived mesenchymal stromal cells. Adult bone marrow-derived mesenchymal stromal cells (BM-MSCs) hold significant therapeutic potential in human and veterinary medicine. Traditionally, BM-MSC cultures use fetal bovine serum (FBS) to promote growth, but this practice introduces batch variability and xenogeneic contamination that complicate clinical translation. While serum-free medium (SFM) alternatives have been optimized for human MSCs, limited options have been explored for alternative or veterinary species. Here, we compared a chemically defined, customizable SFM formulation with conventional serum-containing medium (SCM) fo...
Energy metabolism, replicative ability, intracellular calcium concentration, and ionic channels of horse articular chondrocytes. Some aspects of the physiology of chondrocytes from horse articular cartilage were studied, since this animal model can be helpful in understanding arthritic processes. The replicative ability of articular chondrocytes, measured by the incorporation of [3H]thymidine, and their capacity of proteoglycan production, evaluated from the incorporation of [35S] sulfate, are very low. In addition, these cells do not differentiate in vitro as shown by the constant specific activity of alkaline phosphatase measured at different times in culture. Two types of potassium channels were identified by patch c...
