Virology.
Publisher:
Academic Press.
Frequency: Twenty six no. a year
Country: United States
Language: English
Start Year:1955 -
ISSN:
0042-6822 (Print)
1096-0341 (Electronic)
0042-6822 (Linking)
1096-0341 (Electronic)
0042-6822 (Linking)
Impact Factor
3.7
2022
| NLM ID: | 0110674 |
| (DNLM): | V07900000(s) |
| (OCoLC): | 01769213 |
| Coden: | VIRLAX |
| LCCN: | a 57005753 |
| Classification: | W1 VI828 |
Purification and characterization of equine herpesvirus-induced DNA. Infection of cells with equine herpesvirus type 1 (EHV-1) or type 3 (EHV-3) resulted in the induction of a DNA polymerase activity distinguishable from host cell DNA polymerases by its high salt requirement for maximal activity. By column chromatography on DEAE-cellulose, DNA-cellulose, phosphocellulose, and hydroxyapatite, the EHV-1-induced polymerase was purified 500-fold with 1–2% recovery of total activity from the nuclei of infected hamster livers. The final enzyme preparation was homogeneous as judged by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. Calculations based ...
The nucleic acid content of equine abortion virus. Equine abortion virus was purified from the plasma of infected golden Syrian hamsters by differential centrifugation and nuclease treatment. The preparations were essentially free of nonviral elements on electron microscopic examination, and sedimentation in sucrose and potassium tartrate density gradients resulted in a single visible band. Electron microscopy of this band showed it to be composed of viral particles, and injection into hamsters resulted in infection and death of the animals. The viral particles had a sedimentation coefficient of approximately 2200 S and a hydrated density of 1...